Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 125,227 T→C R737R (CGT→CGC)  aceE → pyruvate dehydrogenase, decarboxylase component E1, thiamine triphosphate‑binding

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913125,2270TC100.0% 121.3 / NA 34R737R (CGT→CGC) aceEpyruvate dehydrogenase, decarboxylase component E1, thiamine triphosphate‑binding
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (18/16);  total (18/16)

CGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTGAAGGTAGCAAAGGTAAAGTTCAGCTGCTCGGCTCCGGTTCTATCCTGCGTCACGTCCGTGAAGCAGCTGAGATCCTGGCGAAAGATTACGGCGTAGGTTCTGACGTTTATAGCGTGACCTCCTTCACCGAGCTGGCGCGTGATGGTCAGGATTGTGAACGCTGGAACATGCTGCAC  >  NC_000913/125091‑125353
                                                                                                                                        |                                                                                                                              
cGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTGAAGGTAGCAAAGGTAAAGTTCAGCTGCTCGGCTCCGGTTCTATCCTGCGCCa                                                                                                                              <  2:531510/139‑1 (MQ=255)
     aaCGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTGAAGGTAGCAAAGGTAAAGTTCAGCTGCTCGGCTCCGGTTCTATCCTGCGCCACGTcc                                                                                                                         >  2:5489/1‑139 (MQ=255)
           aaCTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTGAAGGTAGCAAAGGTAAAGTTCAGCTGCTCGGCTCCGGTTCTATCCTGCGCCACGTCCGTGAag                                                                                                                   <  2:783931/139‑1 (MQ=255)
            aCTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTGAAGGTAGCAAAGGTAAAGTTCAGCTGCTCGGCTCCGGTTCTATCCTGCGCCACGTCCGTGAagc                                                                                                                  <  2:814800/139‑1 (MQ=255)
                     tGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTGAAGGTAGCAAAGGTAAAGTTCAGCTGCTCGGCTCCGGTTCTATCCTGCGCCACGTCCGTGAAGCAGCTGAGAt                                                                                                         <  2:308430/139‑1 (MQ=255)
                              tGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTGAAGGTAGCAAAGGTAAAGTTCAGCTGCTCGGCTCCGGTTCTATCCTGCGCCACGTCCGTGAAGCAGCTGAGATCCTGGCGaa                                                                                                >  2:975000/1‑139 (MQ=255)
                                          cTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTGAAGGTAGCAAAGGTAAAGTTCAGCTGCTCGGCTCCGGTTCTATCCTGCGCCACGTCCGTGAAGCAGCTGAGATCCTGGCGAAAGATTACGGCGt                                                                                    <  2:792888/139‑1 (MQ=255)
                                                  ggTATCCGTAAAGGTATCTACAAACTCGAAACTATTGAAGGTAGCAAAGGTAAAGTTCAGCTGCTCGGCTCCGGTTCTATCCTGCGCCACGTCCGTGAAGCAGCTGAGATCCTGGCGAAAGATTACGGCg                                                                                     <  2:7621/130‑1 (MQ=255)
                                                  ggTATCCGTAAAGGTATCTACAAACTCGAAACTATTGAAGGTAGCAAAGGTAAAGTTCAGCTGCTCGGCTCCGGTTCTATCCTGCGCCACGTCCGTGAAGCAGCTGAGATCCTGGCGAAAGATTACGGCg                                                                                     >  1:7621/1‑130 (MQ=255)
                                                  ggTATCCGTAAAGGTATCTACAAACTCGAAACTATTGAAGGTAGCAAAGGTAAAGTTCAGCTGCACGGCTCCGGTTCTATCCTGCGCCACGTCCGTGAAGCAGCTGAGATCCTGGCGAAAGATTACGGCGTAGGTTCTg                                                                            >  2:789033/1‑139 (MQ=255)
                                                     aTCCGTAAAGGTATCTACAAACTCGAAACTATTGAAGGTAGCAAAGGTAAAGTTCAGCTGCTCGGCTCCGGTTCTATCCTGCGCCACGTCCGTGAAGCAGCTGAGATc                                                                                                        <  1:116971/108‑1 (MQ=255)
                                                     aTCCGTAAAGGTATCTACAAACTCGAAACTATTGAAGGTAGCAAAGGTAAAGTTCAGCTGCTCGGCTCCGGTTCTATCCTGCGCCACGTCCGTGAAGCAGCTGAGATc                                                                                                        >  2:116971/1‑108 (MQ=255)
                                                       ccGTAAAGGTATCTACAAACTCGAAACTATTGAAGGTAGCAAAGGTAAAGTTCAGCTGCTCGGCTCCGGTTCTATCCTGCGCCACGTCCGTGAAGCAGCTGAGATCCTGGCGa                                                                                                 <  1:702810/113‑1 (MQ=255)
                                                       ccGTAAAGGTATCTACAAACTCGAAACTATTGAAGGTAGCAAAGGTAAAGTTCAGCTGCTCGGCTCCGGTTCTATCCTGCGCCACGTCCGTGAAGCAGCTGAGATCCTGGCGa                                                                                                 >  2:702810/1‑113 (MQ=255)
                                                         gTAAAGGTATCTACAAACTCGAAACTATTGAAGGTAGCAAAGGTAAAGTTCAGCTGCTCGGCTCCGGTTCTATCCTGCGCCACGTCCGTGAAGCAGCTGAGATCCTGGCGAAAGATTACGGCGTAGGtt                                                                               <  1:34886/129‑1 (MQ=255)
                                                         gTAAAGGTATCTACAAACTCGAAACTATTGAAGGTAGCAAAGGTAAAGTTCAGCTGCTCGGCTCCGGTTCTATCCTGCGCCACGTCCGTGAAGCAGCTGAGATCCTGGCGAAAGATTACGGCGTAGGtt                                                                               >  2:34886/1‑129 (MQ=255)
                                                          tAAAGGTATCTACAAACTCGAAACTATTGAAGGTAGCAAAGGTAAAGTTCAGCTGCTCGGCTCCGGTTCTATCCTGCGCCACGTCCGTGAAGCAGCTGAGATCCTGGCGAAAGATTACGGCGTAGGTTCTGACGTTtat                                                                    >  2:462032/1‑139 (MQ=255)
                                                                tATCTACAAACTCGAAACTATTGAAGGTAGCAAAGGTAAAGTTCAGCTGCTCGGCTCCGGTTCTATCCTGCGCCACGTCCGTGAAGCAGCTGAGATCCTGGCGAAAGATTACGGCGTAGGTTCTGACGTTTATAGCGTg                                                              >  1:933859/1‑139 (MQ=255)
                                                                tATCTACAAACTCGAAACTATTGAAGGTAGCAAAGGTAAAGTTCAGCTGCTCGGCTCCGGTTCTATCCTGCGCCACGTCCGTGAAGCAGCTGAGATCCTGGCGAAAGATTACGGCGTAGGTTCTGACGTTTATAGCGTg                                                              >  2:741837/1‑139 (MQ=255)
                                                                tATCTACAAACTCGAAACTATTGAAGGTAGCAAAGGTAAAGTTCAGCTGCTCGGCTCCGGTTCTATCCTGCGCCACGTCCGTGAAGCAGCTGAGATCCTGGCGAAAGATTACGGCGTAGGTTCTGACGTTTATAGCGTg                                                              >  2:66949/1‑139 (MQ=255)
                                                                  tCTACAAACTCGAAACTATTGAAGGTAGCAAAGGTAAAGTTCAGCTGCTCGGCTCCGGTTCTATCCTGCGCCACGTCCGTGAAGCAGCTGAGATCCTGGCGAAAGATTACGGCGTAGGTTCTGACGTTTATAGCg                                                                <  1:43071/135‑1 (MQ=255)
                                                                  tCTACAAACTCGAAACTATTGAAGGTAGCAAAGGTAAAGTTCAGCTGCTCGGCTCCGGTTCTATCCTGCGCCACGTCCGTGAAGCAGCTGAGATCCTGGCGAAAGATTACGGCGTAGGTTCTGACGTTTATAGCg                                                                >  2:43071/1‑135 (MQ=255)
                                                                          cTCGAAACTATTGAAGGTAGCAAAGGTAAAGTTCAGCTGCTCGGCTCCGGTTCTATCCTGCGCCACGTCCGTGAAGCAGCTGAGATCCTGGCGAAAGATTACGGCGTAGGTTCTGACGTTTATAGCGTGACCTCCTTCa                                                    >  2:972853/1‑139 (MQ=255)
                                                                               aaCTATTGAAGGTAGCAAAGGTAAAGTTCAGCTGCTCGGCTCCGGTTCTATCCTGCGCCACGTCCGTGAAGCAGCTGAGATCCTGGCGAAAGATTACGGCGTAGGTTCTGACGTTTATAGCGTGACCTCCTTCACCGAg                                               <  1:972853/139‑1 (MQ=255)
                                                                               aaCTATTGAAGGTAGCAAAGGTAAAGTTCAGCTGCTCGGCTCCGGTTCTATCCTGCGCCACGTCCGTGAAGCAGCTGAGATCCTGGCGAAAGATTACGGCGTAGGTTCTGACGTTTATAGCGTGACCTCCTTCACCGAg                                               <  2:13985/139‑1 (MQ=255)
                                                                                         ggTAGCAAAGGTAAAGTTCAGCTGCTCGGCTCCGGTTCTATCCTGCGCCACGTCCGTGAAGCAGCTGAGATCCTGGCGAAAGATTACGGCGTAGGTTCTGACGTTTATAGCGTGACCTCCTTCACCGAGCTGGCGCGTg                                     <  1:66949/139‑1 (MQ=255)
                                                                                              cAAAGGTAAAGTTCAGCTGCTCGGCTCCGGTTCTATCCTGCGCCACGTCCGTGAAGCAGCTGAGATCCTGGCGAAAGATTACGGCGTAGGTTCTGACGTTTATAGCGTGACCTCCTTCACCGAGCTGGCGCGTGATGGt                                >  2:893950/1‑139 (MQ=255)
                                                                                              cAAAGGTAAAGTTCAGCTGCTCGGCTCCGGTTCTATCCTGCGCCACGTCCGTGAAGCAGCTGAGATCCTGGCGAAAGATTACGGCGTAGGTTCTGACGTTTATAGCGTGACCTCCTTCACCGAGCTGGCGCGTGATGGt                                >  1:791014/1‑139 (MQ=255)
                                                                                              cAAAGGTAAAGTTCAGCTGCTCGGCTCCGGTTCTATCCTGCGCCACGTCCGTGAAGCAGCTGAGATCCTGGCGAAAGATTACGGCGTAGGTTATGACGTTTATAGCGTGACCTCCTTCACCGAGCTGGCGCGTGATGGt                                <  2:139067/139‑1 (MQ=255)
                                                                                                 aGGTAAAGTTCAGCTGCTCGGCTCCGGTTCTATCCTGCGCCACGTCCGTGAAGCAGCTGAGATCCTGGCGAAAGATTACGGCGTAGGTTCTGACGTTTATAGCGTGACCTCCTTCACCGAGCTGGCGCGTGATGGTCAg                             >  1:1001446/1‑139 (MQ=255)
                                                                                                           cAGCTGCTCGGCTCCGGTTCTATCCTGCGCCACGTCCGTGAAGCAGCTGAGATCCTGGCGAAAGATTACGGCGTAGGTTCt                                                                             >  1:296188/1‑81 (MQ=255)
                                                                                                           cAGCTGCTCGGCTCCGGTTCTATCCTGCGCCACGTCCGTGAAGCAGCTGAGATCCTGGCGAAAGATTACGGCGTAGGTTCt                                                                             <  2:296188/81‑1 (MQ=255)
                                                                                                             gctgctCGGCTCCGGTTCTATCCTGCGCCACGTCCGTGAAGCAGCTGAGATCCTGGCGAAAGATTACGGCGTAGGTTCTGACGTTTATAGCGTGACCTCCTTCACTGAGCTGGCGCGTGATGGTCAGGATTGTGAACGc                 >  2:703038/1‑139 (MQ=255)
                                                                                                                            ttCTATCCTGCGCCACGTCCGTGAAGCAGCTGAGATCCTGGCGAAAGATTACGGCGTAGGTTCTGACGTTTATAGCGTGACCTCCTTCACCGAGCTGGCGCGTGATGGTCAGGATTGTGAACGCTGGAACATGCTGCAc  <  2:933859/139‑1 (MQ=255)
                                                                                                                                     gcgcCACGTCCGTGAAGCAGCTGAGATCCTGGCGAAAGATTACGGCGTAGGTTCt                                                                             <  1:406849/55‑1 (MQ=255)
                                                                                                                                        |                                                                                                                              
CGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTGAAGGTAGCAAAGGTAAAGTTCAGCTGCTCGGCTCCGGTTCTATCCTGCGTCACGTCCGTGAAGCAGCTGAGATCCTGGCGAAAGATTACGGCGTAGGTTCTGACGTTTATAGCGTGACCTCCTTCACCGAGCTGGCGCGTGATGGTCAGGATTGTGAACGCTGGAACATGCTGCAC  >  NC_000913/125091‑125353

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑