Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,464,489 G→A *317* (TAG→TAA
D7N (GAC→AAC) 
paaX →
paaY →
transcriptional repressor of phenylacetic acid degradation paa operon, phenylacetyl‑CoA inducer
thioesterase required for phenylacetic acid degradation, trimeric, phenylacetate regulatory and detoxification protein, hexapeptide repeat protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,464,4890GA100.0% 114.7 / NA 37*317* (TAG→TAA
D7N (GAC→AAC) 
paaX
paaY
transcriptional repressor of phenylacetic acid degradation paa operon, phenylacetyl‑CoA inducer
thioesterase required for phenylacetic acid degradation, trimeric, phenylacetate regulatory and detoxification protein, hexapeptide repeat protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (18/19);  total (18/19)

CTGCTGCTTTAGCGTTCGTTAGTGAAAAAGGTGAAACCTCGGTCGGTGAACTGCCTGCGCCGGGAAGCCTGTATTTTCAACGTTTTGGCGGCTTGAATATTGAACAGGAGGCGTTATGCCAATTTATCAGATAGACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACAGCGGTATTGATCGGCGATGTTATTCTCGGCAAGGGCGTTTACGTTGGGCCAAATGCCAGCCTGCGTGGCGATTTTGGTCGTATCG  >  NC_000913/1464356‑1464621
                                                                                                                                     |                                                                                                                                    
ctgctgCTTTAGCGTTCGTTAGTGAAAAAGGTGAAACCTCGGTCGGTGAACTGCCTGCGCCGGGAAGCCTGTATTTTCAACGTTTTGGCGGCTTGAATATTGAACAGGAGGCGTTATGCCAATTTATCAGATAAACGGt                                                                                                                                 <  2:427118/139‑1 (MQ=255)
                cgttAGTGAAAAAGGTGAAACCTCGGTCGGTGAACTGCCTGCGCCGGGAAGCCTGTATTTTCAACGTTTTGGCGGCTTGAATATTGAACAGGAGGCGTTATGCCAATTTATCAGATAAACGGTCTGACTCCGGTTGTGc                                                                                                                 <  2:591458/139‑1 (MQ=255)
                             ggTGAAACCTCGGTCGGTGAACTGCCTGCGCCGGGAAGCCTGTATTTTCAACGTTTTGGCGGCTTGAATATTGAACAGGAGGCGTTATGCCAATTTATCAGATAAACGGTCTGACTCCGGTTGTGCCAGAAGAGAGttt                                                                                                    >  1:918543/1‑139 (MQ=255)
                                        ggtcggtGAACTGCCTGCGCCGGGAAGCCTGTATTTTCAACGTTTTGGCGGCTTGAATATTGAACAGGAGGCGTTATGCCAATTTATCAGATAAACGGTCTGACt                                                                                                                           <  2:77614/105‑1 (MQ=255)
                                        ggtcggtGAACTGCCTGCGCCGGGAAGCCTGTATTTTCAACGTTTTGGCGGCTTGAATATTGAACAGGAGGCGTTATGCCAATTTATCAGATAAACGGTCTGACt                                                                                                                           >  1:77614/1‑105 (MQ=255)
                                                    gcctgcGCCGGGAAGCCTGTATTTTCAACGTTTTGGCGGCTTGAATATTGAACAGGAGGCGTTATGCCAATTTATCAGATAAACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACAg                                                                                      <  1:682648/130‑1 (MQ=255)
                                                    gcctgcGCCGGGAAGCCTGTATTTTCAACGTTTTGGCGGCTTGAATATTGAACAGGAGGCGTTATGCCAATTTATCAGATAAACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACAg                                                                                      >  2:682648/1‑130 (MQ=255)
                                                    gcctgcGCCGGGAAGCCTGTATTTTCAACGTTTTGGCGGCTTGAATATTGAACAGGAGGCGTTATGCCAATTTATCAGATAAACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACAGCGGTATTGa                                                                             >  2:67154/1‑139 (MQ=255)
                                                    gcctgcGCCGGGAAGCCTGTATTTTCAACGTTTTGGCGGCTTGAATATTGAACAGGAGGCGTTATGCCAATTTATCAGATAAACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACAGCGGTATTGa                                                                             >  1:913934/1‑139 (MQ=255)
                                                     cctgcGCCGGGAAGCCTGTATTTTCAACGTTTTGGCGGCTTGAATATTGAACAGGAGGCGTTATGCCAATTTATCAGATAAACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACAGCGGTATTGAt                                                                            <  2:918543/139‑1 (MQ=255)
                                                                     tGTATTTTCAACGTTTTGGCGGCTTGAATATTGAACAGGAGGCGTTATGCCAATTTATCAGATAAACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACAGCGGTATTGATCGGCGATGTTATtctc                                                            <  2:913934/139‑1 (MQ=255)
                                                                            tCAACGTTTTGGCGGCTTGAATATTGAACAGGAGGCGTTATGCCAATTTATCAGATAAACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACAGCGGTATTGATCGGCGATGTTATTCTCGGCAAgg                                                     >  1:60572/1‑139 (MQ=255)
                                                                              aaCGTTTTGGCGGCTTGAATATTGAACAGGAGGCGTTATGCCAATTTATCAGATAAACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACAGCGGTATTGATCgg                                                                         >  1:169278/1‑117 (MQ=255)
                                                                              aaCGTTTTGGCGGCTTGAATATTGAACAGGAGGCGTTATGCCAATTTATCAGATAAACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACAGCGGTATTGATCgg                                                                         <  2:169278/117‑1 (MQ=255)
                                                                                    ttGGCGGCTTGAATATTGAACAGGAGGCGTTATGCCAATTTATCAGATAAACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACAGCGGTATTGATCGGCGATGTTATTCTCGGCAAGGGCGTTTAc                                             >  2:101556/1‑139 (MQ=255)
                                                                                                      aaCAGGAGGCGTTATGCCAATTTATCAGATAAACGGTCTGACTCGGGTTGTGCCagaag                                                                                                           >  1:712785/1‑59 (MQ=255)
                                                                                                      aaCAGGAGGCGTTATGCCAATTTATCAGATAAACGGTCTGACTCCGGTTGTGCCagaag                                                                                                           <  2:712785/59‑1 (MQ=255)
                                                                                                           gaggCGTTATGCCAATTTATCAGATAAACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCAt                                                                                             >  1:815195/1‑68 (MQ=255)
                                                                                                           gaggCGTTATGCCAATTTATCAGATAAACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCAt                                                                                             <  2:815195/68‑1 (MQ=255)
                                                                                                           gaggCGTTATGCCAATTTATCAGATAAACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACAGCGGTATTGATCgg                                                                         >  2:939977/1‑88 (MQ=255)
                                                                                                           gaggCGTTATGCCAATTTATCAGATAAACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACAGCGGTATTGATCgg                                                                         <  2:840816/88‑1 (MQ=255)
                                                                                                           gaggCGTTATGCCAATTTATCAGATAAACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACAGCGGTATTGATCgg                                                                         >  1:840816/1‑88 (MQ=255)
                                                                                                           gaggCGTTATGCCAATTTATCAGATAAACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACAGCGGTATTGATCgg                                                                         <  1:939977/88‑1 (MQ=255)
                                                                                                           gaggCGTTATGCCAATTTATCAGATAAACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACAGCGGTATTGATCGGCGATGTTATTCTCGGCAAGGGCGTTTACGTTGGGCCAAATGCCAGCCTGCg                      >  1:28359/1‑139 (MQ=255)
                                                                                                             ggCGTTATGCCAATTTATCAGATAAACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACAGCGGTATTGATCGGCg                                                                       >  2:100626/1‑88 (MQ=255)
                                                                                                             ggCGTTATGCCAATTTATCAGATAAACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACAGCGGTATTGATCGGCg                                                                       <  1:100626/88‑1 (MQ=255)
                                                                                                             ggCGTTATGCCAATTTATCAGATAAACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACAGCGGTATTGATCGGCGATGTTATTCTCGGCa                                                        <  1:738491/103‑1 (MQ=255)
                                                                                                             ggCGTTATGCCAATTTATCAGATAAACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACAGCGGTATTGATCGGCGATGTTATTCTCGGCa                                                        >  2:738491/1‑103 (MQ=255)
                                                                                                              gCGTTATGCCAATTTATCAGATAAACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACAGCGGTATTGATCGGCGATGTTATTCTCGGCAAGGGCGTTTACGTTGGGCCAAATGCCAGCCTGCGTgg                   >  1:983314/1‑139 (MQ=255)
                                                                                                                       cAATTTATCAGATAAACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACAGCGGTATTGATCGGCGATGTTATTCTCGGCAAGGGCGTTTACGTTGGGCCAAATGCCAGCCTGCGTGGCGATTTTgg          <  2:69900/139‑1 (MQ=255)
                                                                                                                         aTTTATCAGATAAACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACAGCGGTATTGATCGGCGATGTTATTCTCGGCAAGGGCGt                                                 <  2:900462/98‑1 (MQ=255)
                                                                                                                         aTTTATCAGATAAACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACAGCGGTATTGATCGGCGATGTTATTCTCGGCAAGGGCGt                                                 >  1:900462/1‑98 (MQ=255)
                                                                                                                              tCAGATAAACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACAGCGGTATTGATCGGCGATGTTATTCTCGGCAAGGGCGTTTACGTTGGGCCAAATGCCAGCCTGCGTGGCGATTTTGGTCGTATc   <  2:408739/139‑1 (MQ=255)
                                                                                                                               cAGATAAACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACAGCGGTATTGATCGGCGATGTTATTCTCGGCAAGGGCGTTTACGTTGGGCCAAATGCCAGCCTGCGTGGCGATTTTGGTCGTATCg  <  2:983314/139‑1 (MQ=255)
                                                                                                                               cAGATAAACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTGTGTCCATCCGACAGCGGTATTTATCGGCGATGTTATTCTCGGCATGGGCTTTTACGTTGGGCCAAATGCCAGTCTGCGTGGCGATTTTGGTCGTATCg  <  1:67154/139‑1 (MQ=255)
                                                                                                                                aGATAAACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACAGCGGTATTGATCGGCGATGTTATTCTCGGCAAGGGCGTTTACGTTGGGCCAAATGCCAGCCTGCGTGGCGATTTTGGTCGTATc   <  2:184211/137‑1 (MQ=255)
                                                                                                                                aGATAAACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACAGCGGTATTGATCGGCGATGTTATTCTCGGCAAGGGCGTTTACGTTGGGCCAAATGCCAGCCTGCGTGGCGATTTTGGTCGTATc   >  1:184211/1‑137 (MQ=255)
                                                                                                                                     |                                                                                                                                    
CTGCTGCTTTAGCGTTCGTTAGTGAAAAAGGTGAAACCTCGGTCGGTGAACTGCCTGCGCCGGGAAGCCTGTATTTTCAACGTTTTGGCGGCTTGAATATTGAACAGGAGGCGTTATGCCAATTTATCAGATAGACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACAGCGGTATTGATCGGCGATGTTATTCTCGGCAAGGGCGTTTACGTTGGGCCAAATGCCAGCCTGCGTGGCGATTTTGGTCGTATCG  >  NC_000913/1464356‑1464621

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: