Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	150,947	T→C	T3A (ACT→GCT)	yadC ←	putative fimbrial‑like adhesin protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	150,947	0	T	C	100.0%	202.8 / NA	59	T3A (ACT→GCT)	yadC	putative fimbrial‑like adhesin protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (33/26);  total (33/26)

AAGGTCATCTGTGCGGCAGTGGAGGGGCCTCGATAGTTATCAACTCCTGCATTATTTCCCACAATAAAACTTGTATATGCACTGACTGTATTACAACAAGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGTCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGTTTTTGTTTGTTATTCGTAGTAAATATCCACTAACACATGGCTCATAAGTTGACCAGATGTAACACTCAATGCAGGATCGATTTTT  >  NC_000913/150811‑151078                                                                                                                                         |                                                                                                                                    aaGGTCATCTGTGCGGCAGTGGAGGGGCCTCGATAGTTATCAACTCCTGCATTATTTCCCACAATAAAACTTGTATATGCACTGACTGTATTACAACAAGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCt                                                                                                                                   >  1:830776/1‑139 (MQ=255)       aTCTGTGCGGCAGTGGAGGGGCCTCGATAGTTATCAACTCCTGCATTATTTCCCACAATAAAACTTGTATATGCACTGACTGTATTACAACAAGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCAttt                                                                                                                             >  1:749641/1‑139 (MQ=255)                          ggCCTCGATAGTTATCAACTCCTGCATTATTTCCCACAATAAAACTTGTATATGCACTGACTGTATTACAACAAGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTa                                                                                                          <  2:830776/139‑1 (MQ=255)                                     ttATCAACTCCTGCATTATTTCCCACAATAAAACTTGTATATGCACTGACTGTATTACAACAAGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGtt                                                                                               >  1:915232/1‑139 (MQ=255)                                       aTCAACTCCTGCATTATTTCCCACAATAAAACTTGTATATGCACTGACTGTATTACAACAAGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATg                                                                                                                  >  2:319842/1‑118 (MQ=255)                                       aTCAACTCCTGCATTATTTCCCACAATAAAACTTGTATATGCACTGACTGTATTACAACAAGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATg                                                                                                                  <  1:319842/118‑1 (MQ=255)                                            cTCCTGCATTATTTCCCACAATAAAACTTGTATATGCACTGACTGTATTACAACAAGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCttt                                                                                                           >  2:397590/1‑120 (MQ=255)                                            cTCCTGCATTATTTCCCACAATAAAACTTGTATATGCACTGACTGTATTACAACAAGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCttt                                                                                                           <  1:397590/120‑1 (MQ=255)                                                tGCATTATTTCCCACAATAAAACTTGTATATGCACTGACTGTATTACAACAAGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGtttt                                                                                    <  1:785351/139‑1 (MQ=255)                                                          cccACAATAAAACTTGTATATGCACTGACTGTATTACAACAAGAATACAGTGCTAAAAAAAGAATGTACCTGAATATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGTTTTTGTTTGTTAt                                                                          <  2:237263/139‑1 (MQ=255)                                                               aaTAAAACTTGTATATGCACTGACTGTATTACAACAAGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGtt                                                                                                     <  1:182220/107‑1 (MQ=255)                                                               aaTAAAACTTGTATATGCACTGACTGTATTACAACAAGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGtt                                                                                                     >  2:182220/1‑107 (MQ=255)                                                                aTAAAACTTGTATATGCACTGACTGTATTACAACAAGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGTTTTTGTTTGTTATTCgtag                                                                    <  2:749641/139‑1 (MQ=255)                                                                               gCACTGACTGTATTACAACAAGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGTTTTTGTTTGTTATTCGTAGTAAATATCCACTAac                                                     >  2:91388/1‑139 (MQ=255)                                                                                cACTGACTGTATTACAACAAGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCtt                                                                                                            <  1:891215/83‑1 (MQ=255)                                                                                cACTGACTGTATTACAACAAGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCtt                                                                                                            >  2:891215/1‑83 (MQ=255)                                                                                  ctgactgTATTACAACAAGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGTTTTTGTTTGTTATTCGTAGTAAATATCCACTAAcaca                                                  <  2:61231/139‑1 (MQ=255)                                                                                  ctgactgTATTACAACAAGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGTTTTTGTTTGTTATTCGTAGTAAATATCCACTAAcaca                                                  <  2:86197/139‑1 (MQ=255)                                                                                    gactgTATTACAACAAGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGTTTTTGTTTGTTATTCGTAGTAAATATCCACTAACACATg                                                >  2:247548/1‑139 (MQ=255)                                                                                     actgTATTACAACAAGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGTTTTTGTTTGTTATTCGTAGTAAATATCCACTAACACATgg                                               >  2:435438/1‑139 (MQ=255)                                                                                          aTTACAACAAGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGTTTTTGTTTGTTATTCGTAGTAAATATCCACTAACACATGGCTCAt                                          >  1:507764/1‑139 (MQ=255)                                                                                               aacaaGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGTTTTTGTTTGTTATTCgtag                                                                    >  1:283130/1‑108 (MQ=255)                                                                                               aacaaGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGTTTTTGTTTGTTATTCgtag                                                                    <  2:283130/108‑1 (MQ=255)                                                                                                 caaGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCaa                                                                                                                    <  2:872807/58‑1 (MQ=255)                                                                                                 caaGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCaa                                                                                                                    >  1:872807/1‑58 (MQ=255)                                                                                                   aGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGTTTTTGTTTGTTATTCgtagta                                                                  <  1:333523/106‑1 (MQ=255)                                                                                                   aGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGTTTTTGTTTGTTATTCgtagta                                                                  >  2:333523/1‑106 (MQ=255)                                                                                                   aGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGTTTTTGTTTGTTATTCGTAGTAAATATCCACTAACACATGGCTCATAAg                                       <  1:190821/133‑1 (MQ=255)                                                                                                   aGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGTTTTTGTTTGTTATTCGTAGTAAATATCCACTAACACATGGCTCATAAg                                       >  2:190821/1‑133 (MQ=255)                                                                                                   aGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGTTTTTGTTTGTTATTCGTAGTAAATATCCACTAACACATGGCTCATAAGTTGAcc                                 >  2:117328/1‑139 (MQ=255)                                                                                                   aGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGTTTTTGTTTGTTATTCGTAGTAAATATCCACTAACACATGGCTCATAAGTTGAcc                                 <  1:242630/139‑1 (MQ=255)                                                                                                         cAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGTTTTTGTTTGTTATTCGTAGTAAATATCCACTAACACATGGCTCATAAGTTGACCAGATGt                           >  1:704249/1‑139 (MQ=255)                                                                                                            tGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGTTTTTGTTTGTTATTCGTATTAAATATCCACTAACACATGGCTCATAAGTTGACCAGATGTAac                        >  1:727450/1‑139 (MQ=255)                                                                                                            tGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGTTTTTGTTTGTTATTCGTAGTAAata                                                              <  1:362791/101‑1 (MQ=255)                                                                                                            tGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGTTTTTGTTTGTTATTCGTAGTAAata                                                              >  2:362791/1‑101 (MQ=255)                                                                                                            tGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGTTTTTGTTTGTTATTCGTAGTAAATATCCACTAACACAt                                                 >  1:578567/1‑114 (MQ=255)                                                                                                            tGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGTTTTTGTTTGTTATTCGTAGTAAATATCCACTAACACAt                                                 <  2:578567/114‑1 (MQ=255)                                                                                                            tGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGTTTTTGTTTGTTATTCGTAGTAAATATCCACTAACACATGGCTCATAAGTTGACCAGATGTAac                        >  2:658033/1‑139 (MQ=255)                                                                                                              cTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGTTTTTGTTTGTTATTCGTAGTAAATATCCACTAACACATGGCTCATAAGTTGAcc                                 <  1:189239/128‑1 (MQ=255)                                                                                                              cTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGATTCTTTATTGTTATGGTTTGCGCTGTTTTTGTTTGTTATTCGTAGTAAATATCCACTAACACATGGCTCATAAGTTGAcc                                 >  2:189239/1‑128 (MQ=255)                                                                                                               tAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGTTTTTGTTTGTTATTCGTAGTAAATATCCACt                                                        >  1:128274/1‑104 (MQ=255)                                                                                                               tAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGTTTTTGTTTGTTATTCGTAGTAAATATCCACt                                                        <  2:128274/104‑1 (MQ=255)                                                                                                                      aGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGTTTTTGTTTGTTATTCGTAGTAAATATCCACTAACACATGGCTGATAAGTTGACCAGATGTa                          <  2:665004/127‑1 (MQ=255)                                                                                                                      aGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGTTTTTGTTTGTTATTCGTAGTAAATATCCACTAACACATGGCTCATAAGTTGAcc                                 >  1:759380/1‑120 (MQ=255)                                                                                                                      aGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGTTTTTGTTTGTTATTCGTAGTAAATATCCACTAACACATGGCTCATAAGTTGAcc                                 <  2:759380/120‑1 (MQ=255)                                                                                                                      aGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGTTTTTGTTTGTTATTCGTAGTAAATATCCACTAACACATGGCTCATAAGTTGACCAGATGTAaca                       <  2:91827/130‑1 (MQ=255)                                                                                                                      aGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGTTTTTGTTTGTTATTCGTAGTAAATATCCACTAACACATGGCTCATAAGTTGACCAGATGTAaca                       >  1:91827/1‑130 (MQ=255)                                                                                                                      aGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGTTTTTGTTTGTTATTCGTAGTAAATATCCACTAACACATGGCTCATAAGTGGACCAGATGTa                          >  1:665004/1‑127 (MQ=255)                                                                                                                      aGAATGTACCTGAAGATAGCCTTCATTTTTACTACACTGTTGCTATATTGTGATGGTTTGTACTGGTTTTGTTTGTTATTCGTAGTAAATATCCACTATCACATAGCTCATAAGTTGACCAGGTGTAACACTCAATGCa              >  1:554565/1‑139 (MQ=255)                                                                                                                        aaTGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGTTTTTGTTTGTTATTCGTAGTAAATATCCACTAACACATGGCt                                             <  2:189863/106‑1 (MQ=255)                                                                                                                        aaTGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGTTTTTGTTTGTTATTCGTAGTAAATATCCACTAACACATGGCt                                             >  1:189863/1‑106 (MQ=255)                                                                                                                                  aaGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGTTTTTGTTTGTTATTCGTAGTAAATATCCACTAACACAt                                                 >  2:197852/1‑92 (MQ=255)                                                                                                                                  aaGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGTTTTTGTTTGTTATTCGTAGTAAATATCCACTAACACAt                                                 <  1:855923/92‑1 (MQ=255)                                                                                                                                  aaGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGTTTTTGTTTGTTATTCGTAGTAAATATCCACTAACACAt                                                 >  2:855923/1‑92 (MQ=255)                                                                                                                                  aaGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGTTTTTGTTTGTTATTCGTAGTAAATATCCACTAACACAt                                                 <  1:197852/92‑1 (MQ=255)                                                                                                                                  aaGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGTTTTTGTTTGTTATTCGTAGTAAATATCCACTAACACATGGCTCATAAGTTGACCAGATGTAACACTCAATGCAGGATCGAttttt  >  2:681848/1‑139 (MQ=255)                                                                                                                                  aaGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGTTTTTGTTTGTTATTCGTAGTAAATATCCACTAACACATGGCTCATAAGTTGACCAGATGTAACACTCAATGCAGGATCGAttttt  >  1:893412/1‑139 (MQ=255)                                                                                                                                      tAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGTTTTTGTTTGTTATTCGTAGTAAATATCCACTAAcaca                                                  <  2:853334/87‑1 (MQ=255)                                                                                                                                      tAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGTTTTTGTTTGTTATTCGTAGTAAATATCCACTAAcaca                                                  >  1:853334/1‑87 (MQ=255)                                                                                                                                         |                                                                                                                                    AAGGTCATCTGTGCGGCAGTGGAGGGGCCTCGATAGTTATCAACTCCTGCATTATTTCCCACAATAAAACTTGTATATGCACTGACTGTATTACAACAAGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGTCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGTTTTTGTTTGTTATTCGTAGTAAATATCCACTAACACATGGCTCATAAGTTGACCAGATGTAACACTCAATGCAGGATCGATTTTT  >  NC_000913/150811‑151078

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑