Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,614,789 C→T intergenic (‑86/‑15) sad ← / → yneJ succinate semialdehyde dehydrogenase, NAD(P)+‑dependent/putative DNA‑binding transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,614,7890CT100.0% 96.9 / NA 31intergenic (‑86/‑15)sad/yneJsuccinate semialdehyde dehydrogenase, NAD(P)+‑dependent/putative DNA‑binding transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (17/14);  total (17/14)

GGCAGGATTTATCGAAATTGCATGAGTTGCCGGAGTAATGGTCATCGGGGTATCTCCTTTATGAGTCATGGTATGAAGATACGCAGATTTACTCTTGCTTTAAAATGAATAATATTAAGCCACTTATTCACGAATCGAGAATGCTATGGATCTGACCCAACTGGAGATGTTCAACGCGGTTGCCGAAGCTGGCAGCATAACCCAGGCTGCAGCAAAAGTGCATCGCGTGCCGTCGAATCTCACTACCCGTCTGCGCCAGCT  >  NC_000913/1614659‑1614919
                                                                                                                                  |                                                                                                                                  
ggCAGGATTTATCGAAATTGCATGAGTTGCCGGAGTAATGGTCATCGGGGTATCTCCTTTATGAGTCATGGTATGAAGATACGCAGATTTACTCTTGCTTTAAAATGAATAATATTAAGCCACTTATTCATGAATCgag                                                                                                                            <  2:191907/139‑1 (MQ=255)
ggCAGGATTTATCGAAATTGCATGAGTTGCCGGAGTAATGGTCATCGGGGTATCTCCTTTATGAGTCATGGTATGAAGATACGCAGATTTACTCTTGCTTTAAAATGAATAATATTAAGCCACTTATTCATGAATCgag                                                                                                                            <  1:963858/139‑1 (MQ=255)
  cAGGATTTATCGAAATTGCATGAGTTGCCGGAGTAATGGTCATCGGGGTATCTCCTTTATGAGTCATGGTATGAAGACACGCAGATTTACTCTTGCTGTAAAATGAATAATATTAAGCCACTTATTCATGAATCGAGaa                                                                                                                          <  2:787323/139‑1 (MQ=255)
                 ttGCATGAGTTGCCGGAGTAATGGTCATCGGGGTATCTCCTTTATGAGTCATGGTATGAAGATACGCAGATTTACTCTTGCTTTAAAATGAATAATATTAAGCCACTTATTCATGAATCGAGAATGCTATGGATCTGAc                                                                                                           <  1:817935/139‑1 (MQ=255)
                                           aTCGGGGTATCTCCTTTATGAGTCATGGTATGAAGATACGCAGATTTACTCTTGCTTTAAAATGAATAATATTAAGCCACTTATTCATGAAt                                                                                                                                <  2:843359/92‑1 (MQ=255)
                                           aTCGGGGTATCTCCTTTATGAGTCATGGTATGAAGATACGCAGATTTACTCTTGCTTTAAAATGAATAATATTAAGCCACTTATTCATGAAt                                                                                                                                >  1:843359/1‑92 (MQ=255)
                                                                gTCATGGTATGAAGATACGCAGATTTACTCTTGCTTTAAAATGAATAATATTAAGCCACTTATTCATGAATCGAGAATGCTATGGATCTGACCCAACTGGAGATGTTCAACGCGGTTGCCGAAGCTGGCAGCATAAccc                                                            >  1:396320/1‑139 (MQ=255)
                                                                  cATGGTATGAAGATACGCAGATTTACTCTTGCTTTAAAATGAATAATATTAAGCCACTTATTCATGAATCGAGAATGCTATGGATCt                                                                                                              <  2:62610/87‑1 (MQ=255)
                                                                  cATGGTATGAAGATACGCAGATTTACTCTTGCTTTAAAATGAATAATATTAAGCCACTTATTCATGAATCGAGAATGCTATGGATCt                                                                                                              >  1:62610/1‑87 (MQ=255)
                                                                  cATGGTATGAAGATACGCAGATTTACTCTTGCTTTAAAATGAATAATATTAAGCCACTTATTCATGAATCGAGAATGCTATGGATCTGACCCAACTGGAGATGTTCAACGCGGTTGCCGAAGCTGGCAGCATAACCCAg                                                          >  1:646223/1‑139 (MQ=255)
                                                                          gAAGATACGCAGATTTACTCTTGCTTTAAAATGAATAATATTAAGCCACTTATTCATGAATCGAGAATGCTa                                                                                                                     <  1:27629/72‑1 (MQ=255)
                                                                          gAAGATACGCAGATTTACTCTTGCTTTAAAATGAATAATATTAAGCCACTTATTCATGAATCGAGAATGCTa                                                                                                                     >  2:27629/1‑72 (MQ=255)
                                                                              aTACGCAGATTTACTCTTGCTTTAAAATGAATAATATTAAGCCACTTATTCATGAATCGAGAATGCTATGGATCTGACCCAACTGGAGATGTTCAACGCGGTTGCCGAAGCTGGCAGCATAACCCAGGCTGCAGCaaaa                                              >  1:518711/1‑139 (MQ=255)
                                                                               tACGCAGATTTACTCTTGCTTTAAAATGAATAATATTAAGCCACTTATTCATGAATCgaga                                                                                                                           <  1:319734/61‑1 (MQ=255)
                                                                               tACGCAGATTTACTCTTGCTTTAAAATGAATAATATTAAGCCACTTATTCATGAATCgaga                                                                                                                           >  2:319734/1‑61 (MQ=255)
                                                                                 cGCAGATTTACTCTTGCTTTAAAATGAATAATATTAAGCCACTTATTCATGAATCGAGAATGCTATGGATCTGAccc                                                                                                         <  2:907475/77‑1 (MQ=255)
                                                                                 cGCAGATTTACTCTTGCTTTAAAATGAATAATATTAAGCCACTTATTCATGAATCGAGAATGCTATGGATCTGAccc                                                                                                         >  1:907475/1‑77 (MQ=255)
                                                                                 cGCAGATTTACTCTTGCTTTAAAATGAATAATATTAAGCCACTTATTCATGAATCGAGAATGCTATGGATCTGACCCAACTGGAGATGTTCAACGCGGTTGCCGAAGCTGGCAGCATAACGCAGGCTGCCGCAAAAGTg                                           >  1:776794/1‑139 (MQ=255)
                                                                                 cGCAGATTTACTCTTGCTTTAAAATGAATAATATTAAGCCACTTATTCATGAATCGAGAATGCTATGGATCTGACCCAACTGGAGATGTTCAACGCGGTTGCCGAAGCTGGCAGCATAACCCAGGCTGCAGCAAAAGTg                                           >  2:446952/1‑139 (MQ=255)
                                                                                                gCTTTAAAATGAATAATATTAAGCCACTTATTCATGAATCGAGAATGCTATGGATCTGACCCAACTGGAGATGTTCAACGCGGTTGCCGAAGCTGgcagca                                                                  >  2:408259/1‑101 (MQ=255)
                                                                                                gCTTTAAAATGAATAATATTAAGCCACTTATTCATGAATCGAGAATGCTATGGATCTGACCCAACTGGAGATGTTCAACGCGGTTGCCGAAGCTGgcagca                                                                  <  1:408259/101‑1 (MQ=255)
                                                                                                gCTTTAAAATGAATAATATTAAGCCACTTATTCATGAATCGAGAATGCTATGGATCTGACCCAACTGGAGATGTTCAACGCGGTTGCCGAAGCTGGCAGCATAACCCAGGCTGCAGCAAAAGTGCATCGCGTGCCGTCg                            >  2:390452/1‑139 (MQ=255)
                                                                                                 cTTTAAAATGAATAATATTAAGCCACTTATTCATGAATCGAGAATGCTATGGATCTGACCCAACTGGAGATGTTCAACGCGGTTGCCGAAGCTGGCAGCATAACCCAGGCTGCAGCAAAAGTGCATCGCGTGCCGTCGa                           <  1:355680/139‑1 (MQ=255)
                                                                                                            ataataTTAAGCCACTTATTCATGAATCGAGAATGCTATGGATCTGACCCAACTGGAGATGTTCAACGCGGTTGCCGAAGCTGGCAGCATAACCCAGGCTGCAGCAAAAGTGCATCGCGTGCCGTCGAATCTCACTAcc                >  1:659129/1‑139 (MQ=255)
                                                                                                                tatTAAGCCACTTATTCATGAATCGAGAATGCTATGGATCTGAc                                                                                                           >  1:75531/1‑44 (MQ=255)
                                                                                                                tatTAAGCCACTTATTCATGAATCGAGAATGCTATGGATCTGAc                                                                                                           <  2:75531/44‑1 (MQ=255)
                                                                                                                     aGCCACTTATTCATGAATCGAGAATGCTATGGATCTGACCCAACTGGAGATGTTCAACGCGGTTGCCGAAGCTGGCAGCATAACCCAGGCTGCAGCAAAAGTGCATCGCGTGCCGTCGAATCTCACTACCCGTCTgcgc       >  2:179422/1‑139 (MQ=255)
                                                                                                                     aGCCACTTATTCATGAATCGAGAATGCTATGGATCTGACCCAACTGGAGATGTTCAACGCGGTTGCCGAAGCTGGCAGCATAACCCAGGCTGCAGCAAAAGTGCATCGCGTGCCGTCGAATCTCACTACCCGTCTgcgc       >  2:341085/1‑139 (MQ=255)
                                                                                                                     aGCCACTTATTCATGAATCGAGAATGCTATGGATCTGACCCAACTGGAGATGTTCAACGCGGTTGCCGAAGCTGGCAGCATAACCCAGGCTGCAGCAAAAGTGCATCGCGTGCCGTCGAATCTCACTACCCGTCTgcgc       <  1:446952/139‑1 (MQ=255)
                                                                                                                     aGCCACTTATTCATGAATCGAGAATGCTATGGATCTGACCCAACTGGAGATGTTCAACGCGGTTGCCGAAGCTGGCAGCATAACCCAGGCTGCAGCAAAAGTGCATCGCGTGCCGTCGAATCTCACTACCCGTCTgcgc       >  1:412526/1‑139 (MQ=255)
                                                                                                                          cTTATTCATGAATCGAGAATGCTATGGATCTGACCCAACTGGAGATGTTCAACGCGGTTGCCGAAGCTGGCAGCATAACCCAGGCTGCAGCAAAAGTGCATCGCGTGCCGTCGAATCTCACTACCCGTCTGCGCCAGCt  <  2:659129/139‑1 (MQ=255)
                                                                                                                                  |                                                                                                                                  
GGCAGGATTTATCGAAATTGCATGAGTTGCCGGAGTAATGGTCATCGGGGTATCTCCTTTATGAGTCATGGTATGAAGATACGCAGATTTACTCTTGCTTTAAAATGAATAATATTAAGCCACTTATTCACGAATCGAGAATGCTATGGATCTGACCCAACTGGAGATGTTCAACGCGGTTGCCGAAGCTGGCAGCATAACCCAGGCTGCAGCAAAAGTGCATCGCGTGCCGTCGAATCTCACTACCCGTCTGCGCCAGCT  >  NC_000913/1614659‑1614919

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: