| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,621,542 | G→A | D71N (GAC→AAC) | ydeE → | putative transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,621,542 | 0 | G | A | 100.0% | 103.1 / NA | 33 | D71N (GAC→AAC) | ydeE | putative transporter |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (16/17); total (16/17) | |||||||||||
GCTGCCATTTATGACCATTTACTTGAGTCGCCAGTACAGCCTGAGTGTCGATCTAATCGGTTATGCGATGACAATTGCGCTCACTATTGGCGTCGTTTTTAGCCTCGGTTTTGGTATCCTGGCGGATAAGTTCGACAAGAAACGCTATATGTTACTGGCAATTACCGCCTTCGCCAGCGGTTTTATTGCCATTACTTTAGTGAATAACGTGACGCTGGTTGTGCTCTTTTTTGCCCTCATTAACTGCGC > NC_000913/1621409‑1621657 | gCTGCCATTTATGACCATTTACTTGAGTCGCCAGTACAGCCTGAGTGTCGATCTAATCGGTTATGCGATGACAATTGCGCTCACTATTGGCGTCGTTTTTAGCCTCGGTTTTGGTATCCTGGCGGATAAGTTCAACAAg < 2:390787/139‑1 (MQ=255) tttATGACCATTTACTTGAGTCGCCAGTACAGCCTGAGTGTCGATCTAATCGGTTATGCGATGACAATTGCGCTCACTATTGGCGTCGTTTTTAGCCTCGGTTTTGGTATCCTGGCGGATAAGTTCAACCAGAAACGCt < 2:749367/139‑1 (MQ=255) gtgtCGATCTAATCGGTTATGCGATGACAATTGCGCTCACTATTGGCGTCGTTTTTAGCCTCGGTTTTGGTATCCTGGCGGATAAGTTCAACAAGAAACGCTATATGTTAc < 2:514882/111‑1 (MQ=255) gtgtCGATCTAATCGGTTATGCGATGACAATTGCGCTCACTATTGGCGTCGTTTTTAGCCTCGGTTTTGGTATCCTGGCGGATAAGTTCAACAAGAAACGCTATATGTTAc > 1:514882/1‑111 (MQ=255) tgtCGATCTAATCGGTTATGCGATGACAATTGCGCTCACTATTGGCGTCGTTTTTAGCCTCGGTTTTGGTATCCTGGCGGATAAGTTCAACAAGAAACGCTATATGTTACTGGCAATTACCGCCTTCGCCAGCGGtttt < 1:689356/139‑1 (MQ=255) cGATCTAATCGGTTATGCGATGACAATTGCGCTCACTATTGGCGTCGTTTTTAGCCTCGGTTTTGGTATCCTGGCGGATAAGTTCAACAAGAAACGCTATATGTTACTGGCAATTACCGCCTTCGCCAGCGGTTTTAtt < 2:49951/139‑1 (MQ=255) ttATGCGATGACAATTGCGCTCACTATTGGCGTCGTTTTTAGCCTCGGTTTTGGTATCCTGGCGGATAAGTTCAACAAGAAACGCTATATGTTACTGGCAATTACCGCCTTCGCCAGCGGTTTTATTGCCATTACTTTa > 2:369603/1‑139 (MQ=255) tATGCGATGACAATTGCGCTCACTATTGGCGTCGTTTTTAGCCTCGGTTTTGGTATCCTGGCGGATAAGTTCAACAAGAAACGCTATATGTTACTGGCAATTACCGCCTTCGCCAGCGGtttt < 1:112300/123‑1 (MQ=255) tATGCGATGACAATTGCGCTCACTATTGGCGTCGTTTTTAGCCTCGGTTTTGGTATCCTGGCGGATAAGTTCAACAAGAAACGCTATATGTTACTGGCAATTACCGCCTTCGCCAGCGGtttt > 2:112300/1‑123 (MQ=255) tATGCGATGACAATTGCGCTCACTATTGGCGTCGTTTTTAGCCTCGGTTTTGGTATCCTGGCGGATAAGTTCAACAAGAAACGCTATATGTTACTGGCAATTACCGCCTTCGCCAGCGGTTTTATTGCCATTACTTTAg > 1:584684/1‑139 (MQ=255) tGCGATGACAATTGCGCTCACTATTGGCGTCGTTTTTAGCCTCGGTTTTGGTATCCTGGCGGATAAGTTCAACAAGAAACGCTATATGTTACTGGCAATTACCGCCTTCGCCAGCGGTTTTATTGCCATTACt > 1:737872/1‑133 (MQ=255) tGCGATGACAATTGCGCTCACTATTGGCGTCGTTTTTAGCCTCGGTTTTGGTATCCTGGCGGATAAGTTCAACAAGAAACGCTATATGTTACTGGCAATTACCGCCTTCGCCAGCGGTTTTATTGCCATTACt > 2:411271/1‑133 (MQ=255) tGCGATGACAATTGCGCTCACTATTGGCGTCGTTTTTAGCCTCGGTTTTGGTATCCTGGCGGATAAGTTCAACAAGAAACGCTATATGTTACTGGCAATTACCGCCTTCGCCAGCGGTTTTATTGCCATTACt < 2:737872/133‑1 (MQ=255) tGCGATGACAATTGCGCTCACTATTGGCGTCGTTTTTAGCCTCGGTTTTGGTATCCTGGCGGATAAGTTCAACAAGAAACGCTATATGTTACTGGCAATTACCGCCTTCGCCAGCGGTTTTATTGCCATTACt < 1:411271/133‑1 (MQ=255) cTCACTATTGGCGTCGTTTTTAGCCTCGGTTTTGGTATCCTGGCGGATAAGTTCAACAAGAAACg < 1:422930/65‑1 (MQ=255) cTCACTATTGGCGTCGTTTTTAGCCTCGGTTTTGGTATCCTGGCGGATAAGTTCAACAAGAAACg > 2:422930/1‑65 (MQ=255) aCTATTGGCGTCGTTTTTAGCCTCGGTTTTGGTATCCTGGCGGATAAGTTCAACAAGAAACGCTATATGTTACTGGCAATTACCGCCTTCGCCAGCGGTTTTa > 1:450095/1‑103 (MQ=255) aCTATTGGCGTCGTTTTTAGCCTCGGTTTTGGTATCCTGGCGGATAAGTTCAACAAGAAACGCTATATGTTACTGGCAATTACCGCCTTCGCCAGCGGTTTTa < 2:450095/103‑1 (MQ=255) tcgtTTTTAGCCTCGGTTTTGGTATCCTGGCGGATAAGTTCAACAAGAAACGCtat > 2:251765/1‑56 (MQ=255) tcgtTTTTAGCCTCGGTTTTGGTATCCTGGCGGATAAGTTCAACAAGAAACGCtat < 1:251765/56‑1 (MQ=255) tcgtTTTTAGCCTCGGTTTTGGTATCCTGGCGGATAAGTTCAACAAGAAACGCTATATGTTACTGGCAATTa < 1:52859/72‑1 (MQ=255) tcgtTTTTAGCCTCGGTTTTGGTATCCTGGCGGATAAGTTCAACAAGAAACGCTATATGTTACTGGCAATTa > 2:52859/1‑72 (MQ=255) gtTTTTAGCCTCGGTTTTGGTATCCTGGCGGATAAGTTCAACAAGAAACGCTATATGTTACTGGCAATTACCGCCTTCGCCAGCGGtttt < 1:543911/90‑1 (MQ=255) gtTTTTAGCCTCGGTTTTGGTATCCTGGCGGATAAGTTCAACAAGAAACGCTATATGTTACTGGCAATTACCGCCTTCGCCAGCGGtttt > 2:543911/1‑90 (MQ=255) gtTTTTAGCCTCGGTTTTGGTATCCTGGCGGATAAGTTCAACAAGAAACGCTATATGTTACTGGCAATTACCGCCTTCGCCAGCGGTTTTATTGCCATTACTTTAGTGAATAACGTGACGCTGGTTGTGCTCTTTTTTg > 2:839674/1‑139 (MQ=255) gtTTTTAGCCTCGGTTTTGGTATCCTGGCGGATAAGTTCAACAAGAAACGCTATATGTTACTGGCAATTACCGCCTTCGCCAGCGGTTTTATTGCCATTACTTTAGTGAATAACGTGACGCTGGTTGTGCTCTTTTTTg > 2:813305/1‑139 (MQ=255) gtTTTTAGCCTCGGTTTTGGTATCCTGGCGGATAAGTTCAACAAGAAACGCTATATGTTACTGGCAATTACCGCCTTCGCCAGCGGTTTTATTGCCATTACTTTAGTGAATAACGTGACGCTGGTTGTGCTCTTTTTTg > 2:659528/1‑139 (MQ=255) gtTTTTAGCCTCGGTTTTGGTATCCTGGCGGATAAGTTCAACAAGAAACGCTATATGTTACTGGCAATTACCGCCTTCGCCAGCGGTTTTATTGCCATTACTTTAGTGAATAACGTGACGCTGGTTGTGCTCTTTTTTg < 2:584684/139‑1 (MQ=255) cGGTTTTGGTATCCTGGCGGATAAGTTCAACAAGAAACGCTATATGTTACTGGCAATTACCGCCTTCGCCAGCGGTTTTATTGCCATTACTTTAGTGaa < 1:620766/99‑1 (MQ=255) aggTTTTGGTATCCTGGCGGATAAGTTCAACAAGAAACGCTATATGTTACTGGCAATTACCGCCTTCGCCAGCAGTTTTATTGCCATTACTTTAGTGaa > 2:620766/2‑99 (MQ=255) ttGGTATCCTGGCGGATAAGTTCAACAAAAAACGCTATATGGTACTGGCAATTACCGCCTTCGCCAGCGGTTTTATTGCCATTACTTTAGTGAATAACGTGACGCTGGTTGTGCTCTTTTTTGCCCTCATTAACTgggc < 1:140559/139‑1 (MQ=255) ccTGGCGGATAAGTTCAACAAGAAACGCTATATGTTACTGGCAATTACCGCCTTCGCCAGCGGTTTTATTGCCATTACt > 2:394224/1‑79 (MQ=255) ccTGGCGGATAAGTTCAACAAGAAACGCTATATGTTACTGGCAATTACCGCCTTCGCCAGCGGTTTTATTGCCATTACt < 1:394224/79‑1 (MQ=255) | GCTGCCATTTATGACCATTTACTTGAGTCGCCAGTACAGCCTGAGTGTCGATCTAATCGGTTATGCGATGACAATTGCGCTCACTATTGGCGTCGTTTTTAGCCTCGGTTTTGGTATCCTGGCGGATAAGTTCGACAAGAAACGCTATATGTTACTGGCAATTACCGCCTTCGCCAGCGGTTTTATTGCCATTACTTTAGTGAATAACGTGACGCTGGTTGTGCTCTTTTTTGCCCTCATTAACTGCGC > NC_000913/1621409‑1621657 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |