Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,726,622 G→A *200* (TAG→TAA nemR → transcriptional repressor for the nemRA‑gloA operon, quinone‑, glyoxal‑, and HOCl‑activated

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,726,6220GA100.0% 47.2 / NA 16*200* (TAG→TAAnemRtranscriptional repressor for the nemRA‑gloA operon, quinone‑, glyoxal‑, and HOCl‑activated
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (8/8);  total (8/8)

GCACAAGTGCTTTACGCACTGTGGCTTGGCGCGAATCTGCAGGCCAAAATTTCGCGCAGTTTCGAGCCACTGGAAAACGCGCTGGCCCATGTAAAAAACATTATTGCGACGCCTGCCGTTTAGCAGGCATTTTTTATCACCAGACGACCGGGAGCCTTTATGTCATCTGAAAAACTGTATTCCCCACTGAAAGTGGGCGCGATCACGGCGGCAAACCGTATTTTTATGGCACCGCTGACG  >  NC_000913/1726500‑1726739
                                                                                                                          |                                                                                                                     
gCACAAGTGCTTTACGCACTGTGGCTTGGCGCGAATCTGCAGGCCAAAATTTCGCGCAGTTTCGAGCCACTGGAAAACGCGCTGGCCCATGTAAAAAACATTATTGCGACGCCTGCCGTTTAACAGGCATTTTTTATCa                                                                                                       >  1:705220/1‑139 (MQ=255)
               gCACTGTGGCTTGGCGCGAATCTGCAGGCCAAAATTTCGCGCAGTTTCGAGCCACTGGAAAACGCGCTGGCCCATGTAAAAAACATTATTGCGACGCCTGCCGTTTAACAGGCATTTTTTATCACCAGACGACCGGGAg                                                                                        <  2:705220/139‑1 (MQ=255)
                 aCTGTGGCTTGGCGCGAATCTGCAGGCCAAAATTTCGCGCAGTTTCGAGCCACTGGAAAACGCGCTGGCCCATGTAAAAAACATTATTGCGACGCCTGCCGTTTAACAGGCATTTTTTATCACCAGACGACCGGGAGcc                                                                                      >  1:545854/1‑139 (MQ=255)
                     tgGCTTGGCGCGAATCTGCAGGCCAAAATTTCGCGCAGTTTCGAGCCACTGGAAAACGCTCTGGCCCATGTAAAAAACATTATTGCGACGCCTGCCGTTTAACAGGCATTTTTTATCACCAGACGACCGGGAGCCTTTa                                                                                  >  1:625680/1‑139 (MQ=255)
                        cTTGGCGCGAATCTGCAGGCCAAAATTTCGCGCAGTTTCGAGCCACTGGAAAACGCGCTGGCCCATGTAAAAAACATTATTGCGACGCCTGCCGTTTAACAGGCATTTTTTATCACCAGACGACCGGGAGCCTTTATGt                                                                               >  1:787849/1‑139 (MQ=255)
                                   tCTGCAGGCCAAAATTTCGCGCAGTTTCGAGCCACTGGAAAACGCGCTGGCCCATGTAAAAAACATTATTGCGACGCCTGCCGTTTAACAGGCATTTTTTATCACCAGACGACCGGGAGCCTTTATGTCATCTGaaaaa                                                                    <  2:143933/139‑1 (MQ=255)
                                        aGGCCAAAATTTCGCGCAGTTTCGAGCCACTGGAAAACGCGCTGGCCCATGTAAAAAACATTATTGCGACGCCTGCCGTTTAACAGGCATTTTTTATCACCAGACGACCGGGAGCCTTTATGTCATCTGAAAAACTGTa                                                               <  2:545854/139‑1 (MQ=255)
                                                                  ccACTGGAAAACGCGCTGGCCCATGTAAAAAACATTATTGCGACGCCTGCCGTTTAACAGGCATTTTTTATCACCAGACGACCGGGAGCCTTTATGTCATCTGAAAAACTGTATTCCCCACTGAAAGTGGGCGCGATCa                                     <  1:415753/139‑1 (MQ=255)
                                                                            aCGCTCTGGCCCATGTAAAAAACATTATTGCGACGCCTGCCGTTTAACAGGCATTTTTTATCACCAGACGACCGGGAGCCTTTATGTCATCTGAAAAACTGTATTCCCCAGTGAAAGTGGGCGCGATCACGGCGGCaaa                           <  2:625680/139‑1 (MQ=255)
                                                                                                    ttattGCGACGCCTGCCGTTTAACAGGCATTTTTTATCACCAGACGACCGGGAGCCTTTATGTCATCTGAAAAACTGTATTCCCCACTGAAAGTGGGCGCGATCACGGCGGCAAACCGTAt                     >  1:515963/1‑121 (MQ=255)
                                                                                                    ttattGCGACGCCTGCCGTTTAACAGGCATTTTTTATCACCAGACGACCGGGAGCCTTTATGTCATCTGAAAAACTGTATTCCCCACTGAAAGTGGGCGCGATCACGGCGGCAAACCGTAt                     <  2:515963/121‑1 (MQ=255)
                                                                                                     tattGCGACGCCTGCCGTTTAACAGGCATTTTTTATCACCAGACGACCGGGAGCCTTTATGTCATCTGAAAAACTGTATTCCCCACTGAAAGTGGGCGCGATCACGGCGGCAAACCGTATTTTTATGGCACCGCTGAcg  >  1:59155/1‑139 (MQ=255)
                                                                                                          cGACGCCTGCCGTTTAACAGGCATTTTTTATCACCAGACGACCggg                                                                                          >  1:888790/1‑46 (MQ=255)
                                                                                                          cGACGCCTGCCGTTTAACAGGCATTTTTTATCACCAGACGACCggg                                                                                          <  2:888790/46‑1 (MQ=255)
                                                                                                              gCCTGCCGTTTAACAGGCATTTTTTATCACCAGACGACCGGGAGCCTTTATGTCAt                                                                            >  1:769977/1‑56 (MQ=255)
                                                                                                              gCCTGCCGTTTAACAGGCATTTTTTATCACCAGACGACCGGGAGCCTTTATGTCAt                                                                            <  2:769977/56‑1 (MQ=255)
                                                                                                                          |                                                                                                                     
GCACAAGTGCTTTACGCACTGTGGCTTGGCGCGAATCTGCAGGCCAAAATTTCGCGCAGTTTCGAGCCACTGGAAAACGCGCTGGCCCATGTAAAAAACATTATTGCGACGCCTGCCGTTTAGCAGGCATTTTTTATCACCAGACGACCGGGAGCCTTTATGTCATCTGAAAAACTGTATTCCCCACTGAAAGTGGGCGCGATCACGGCGGCAAACCGTATTTTTATGGCACCGCTGACG  >  NC_000913/1726500‑1726739

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: