Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,735,484	T→C	V36A (GTC→GCC)	sodB →	superoxide dismutase, Fe

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,735,484	0	T	C	100.0%	175.3 / NA	48	V36A (GTC→GCC)	sodB	superoxide dismutase, Fe
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (23/25);  total (23/25)

ATGCAAATTAATAATAAAGGAGAGTAGCAATGTCATTCGAATTACCTGCACTACCATATGCTAAAGATGCTCTGGCACCGCACATTTCTGCGGAAACCATCGAGTATCACTACGGCAAGCACCATCAGACTTATGTCACTAACCTGAACAACCTGATTAAAGGTACCGCGTTTGAAGGTAAATCACTGGAAGAGATTATTCGCAGCTCTGAAGGTGGCGTATTCAACAACGCAGCTCAGGTCTGGAACCATACTTTCTACTGGAA  >  NC_000913/1735349‑1735613                                                                                                                                        |                                                                                                                                  aTGCAAATTAATAATAAAGGAGAGTAGCAATGTCATTCGAATTACCTGCACTACCATATGCTAAAGATGCTCTGGCACCGCACATTTCTGCGGAAACCATCGAGTATCACTACGGCAAGCACCATCAGACTTATGCCAc                                                                                                                                >  2:789656/1‑139 (MQ=255)            taataaAGGAGAGTAGCAATGTCATTCGAATTACCTGCACTACCATATGCTAAAGATGCTCTGGCACCGCACATTTCTGCGGAAACCATCGAGTATCACTACGGCAAGCACCATCAGACTTATGCCACTAAc                                                                                                                            >  2:839157/1‑132 (MQ=255)            taataaAGGAGAGTAGCAATGTCATTCGAATTACCTGCACTACCATATGCTAAAGATGCTCTGGCACCGCACATTTCTGCGGAAACCATCGAGTATCACTACGGCAAGCACCATCAGACTTATGCCACTAAc                                                                                                                            <  1:839157/132‑1 (MQ=255)             aataaAGGAGAGTAGCAATGTCATTCGAATTACCTGCACTACCATATGCTAAAGATGCTCTGGCACCGCACATTTCTGCGGAAACCATCGAGTATCACTACGGCAAGCACCATCAGACTTATGCCACTAACCTGaacaa                                                                                                                    <  2:581089/139‑1 (MQ=255)                    gagaGTAGCAATGTCATTCGAATTACCTGCACTACCATATGCTAAAGATGCTCTGGCACCGCACATTTCTGCGGAAACCATCGAGTATCACTACGGCAAGCACCATCAGACTTATGCCACTAACCTGAACAACCTGAtt                                                                                                             <  2:7176/139‑1 (MQ=255)                           gCAATGTCATTCGAATTACCTGCACTACCATATGCTAAAGATGCTCTGGCACCGCACATTTCTGCGGAAACCATCGAGTATCACTACGGCAAGCACCATCAGACTTATGCCACTAACCTGAACAACCTGATTAAAGGTa                                                                                                      >  2:222277/1‑139 (MQ=255)                                 tCATTCGAATTACCTGCACTACCATATGCTAAAGATGCTCTGGCACCGCACATTTCTGCGGAAACCATCGAGTATCACTACGGCAAGCACCATCAGACTTATGCCACTAACCTGAACAACCTGATTAAAGGTACCGCGt                                                                                                >  2:534639/1‑139 (MQ=255)                                    ttCGAATTACCTGCACTACCATATGCTAAAGATGCTCTGGCACCGCACATTTCTGCGGAAACCATCGAGTATCACTACGGCAAGCACCATCAGACTTATGCCACTAACCTGAACAACCTGATTAAAGGTACCGCGTTTg                                                                                             <  1:142173/139‑1 (MQ=255)                                                  aCTACCATATGCTAAAGATGCTCTGGCACCGCACATTTCTGCGGAAACCATCGAGTATCACTACGGCAAGCACCATCAGACTTATGCCACTAACCTGAACAACCTGATTAAAGGTACCGCGTTTGAAGGTAAATCACTg                                                                               >  1:947382/1‑139 (MQ=255)                                                    tACCATATGCTAAAGATGCTCTGGCACCGCACATTTCTGCGGAAACCATCGAGTATCACTACGGCAAGCACCATCAGACTTATGCCACTAACCTGAACAACCTGATTAAAGGTACCGCGTTTGAAGGTAAATCACTGGa                                                                             <  1:222277/139‑1 (MQ=255)                                                      ccATATGCTAAAGATGCTCTGGCACCGCACATTTCTGCGGAAACCATCGAGTATCACTACGGCAAGCACCATCAGACTTATGCCACTAACCTGAACAACCTGATTAAAGGTACCGCGTTTGAAGGTAGATCACTGGAag                                                                           >  2:661392/1‑139 (MQ=255)                                                           tGCTAAAGATGCTCTGGCACCGCACATTTCTGCGGAAACCATCGAGTATCACTACGGCAAGCACCATCAGACTTATGCCACTAACCTGa                                                                                                                        <  2:371909/89‑1 (MQ=255)                                                           tGCTAAAGATGCTCTGGCACCGCACATTTCTGCGGAAACCATCGAGTATCACTACGGCAAGCACCATCAGACTTATGCCACTAACCTGa                                                                                                                        >  1:371909/1‑89 (MQ=255)                                                           tGCTAAAGATGCTCTGGCACCGCACATTTCTGCGGAAACCATCGAGTATCACTACGGCAAGCACCATCAGACTTATGCCACTAACCTGAACAACCTGATTAAAGGTAcc                                                                                                    <  1:617266/109‑1 (MQ=255)                                                           tGCTAAAGATGCTCTGGCACCGCACATTTCTGCGGAAACCATCGAGTATCACTACGGCAAGCACCATCAGACTTATGCCACTAACCTGAACAACCTGATTAAAGGTAcc                                                                                                    >  2:617266/1‑109 (MQ=255)                                                              tAAAGATGCTCTGGCACCGCACATTTCTGCGGAAACCATCGAGTATCACTACGGCAAGCACCATCAGACTTATGCCACTAACCTGAACAACCTGATTAAAGGTACCGCGTTTGAAGGTaa                                                                                      >  1:516249/1‑120 (MQ=255)                                                              tAAAGATGCTCTGGCACCGCACATTTCTGCGGAAACCATCGAGTATCACTACGGCAAGCACCATCAGACTTATGCCACTAACCTGAACAACCTGATTAAAGGTACCGCGTTTGAAGGTaa                                                                                      <  2:516249/120‑1 (MQ=255)                                                                aaGATGCTCTGGCACCGCACATTTCTGCGGAAACCATCGAGTATCACTACGGCAAGCACCATCAGACTTATGCCACTAACCTGAACAACCTGATTAAAGGt                                                                                                       >  1:15491/1‑101 (MQ=255)                                                                aaGATGCTCTGGCACCGCACATTTCTGCGGAAACCATCGAGTATCACTACGGCAAGCACCATCAGACTTATGCCACTAACCTGAACAACCTGATTAAAGGt                                                                                                       <  2:15491/101‑1 (MQ=255)                                                                aaGATGCTCTGGCACCGCACATTTCTGCGGAAACCATCGAGTATCACTACGGCAAGCACCATCAGACTTATGCCACTAACCTGAACAACCTGATTAAAGGTACCGCGTTTGAAGGTAAATCACTGGAAGAGATTATTCg                                                                 >  1:75434/1‑139 (MQ=255)                                                                aaGATGCTCTGGCACCGCACATTTCTGCGGAAACCATCGAGTATCACTACGGCAAGCACCATCAGACTTATGCCACTAACCTGAACAACCTGATTAAAGGTACCGCGTTTGAAGGTAAATCACTGGAAGAGATTATTCg                                                                 >  1:95086/1‑139 (MQ=255)                                                                       tctGGCACCGCACATTTCTGCGGAAACCATCGAGTATCACTACGGCAAGCACCATCAGACTTATGCCACTAACCTGAACAACCTGATTAAAGGTACCGCGTTTGAAGGTAAATCACTGGAAGAGATTATTCGCAGctct                                                          >  2:96293/1‑139 (MQ=255)                                                                       tctGGCACCGCACATTTCTGCGGAAACCATCGAGTATCACTACGGCAAGCACCATCAGACTTATGCCACTAACCTGAACAACCTGATTAAAGGTACCGCGTTTGAAGGTAAATCACTGGAAGAGATTATTCGCAGctct                                                          <  1:197373/139‑1 (MQ=255)                                                                              ccGCACATTTCTGCGGAAACCATCGAGTATCACTACGGCAAGCACCATCAGACTTATGCCACTAACCTGAACAACCTGATTAAAGGTACCGCGTTTGAAGGTAAATCACTGGAAGAGATTATTCGCAGCTCTGAAGGTg                                                   <  2:75434/139‑1 (MQ=255)                                                                                gCACATTTCTGCGGAAACCATCGAGTATCACTACGGCAAGCACCATCAGACTTATGCCACTAACCTGAACAACCTGATTAAAGGTACCGCGTTTGAAGGTAAATc                                                                                   <  2:996327/105‑1 (MQ=255)                                                                                gCACATTTCTGCGGAAACCATCGAGTATCACTACGGCAAGCACCATCAGACTTATGCCACTAACCTGAACAACCTGATTAAAGGTACCGCGTTTGAAGGTAAATc                                                                                   >  1:996327/1‑105 (MQ=255)                                                                                gCACATTTCTGCGGAAACCATCGAGTATCACTACGGCAAGCACCATCAGACTTATGCCACTAACCTGAACAACCTGATTAAAGGTACCGCGTTTGAAGGTAAATCACTGGAAGAGATTATTCGCAGCTCTGAAGGTGGc                                                 <  1:789656/139‑1 (MQ=255)                                                                                 cacaTTTCTGCGGAAACCATCGAGTATCACTACGGCAAGCACCATCAGACTTATGCCACTAACCTGAACAACCTGATTAAAGGTACCGCGTTTGAAGGTAAATCACTGGAAGAGATTATTCGCAGCTCTGAAg                                                      <  2:476231/133‑1 (MQ=255)                                                                                 cacaTTTCTGCGGAAACCATCGAGTATCACTACGGCAAGCACCATCAGACTTATGCCACTAACCTGAACAACCTGATTAAAGGTACCGCGTTTGAAGGTAAATCACTGGAAGAGATTATTCGCAGCTCTGAAg                                                      >  1:476231/1‑133 (MQ=255)                                                                                 cacaTTTCTGCGGAAACCATCGAGTATCACTACGGCAAGCACCATCAGACTTATGCCACTAACCTGAACAACCTGATTAAAGGTACCGCGTTTGAAGGTAAATCACTGGAAGAGATTATTCGCAGCTCTGAAGGTGGCg                                                >  2:456076/1‑139 (MQ=255)                                                                                   caTTTCTGCGGAAACCATCGAGTATCACTACGGCAAGCACCATCAGACTTATGCCACTAACCTGAACAACCTGATTAAAGGTACCGCGTTTGAAGGTAAATCACTGGAAGAGATTATTCGCAGCTCTGAAGGTGGCGTa                                              <  2:902072/139‑1 (MQ=255)                                                                                                aCCATCGAGTATCACTACGGCAAGCACCATCAGACTTATGCCACTAACCTGAACAAcc                                                                                                                  >  2:209026/1‑58 (MQ=255)                                                                                                aCCATCGAGTATCACTACGGCAAGCACCATCAGACTTATGCCACTAACCTGAACAAcc                                                                                                                  <  1:209026/58‑1 (MQ=255)                                                                                                aCCATCGAGTATCACTACGGCAAGCACCATCAGACTTATGCCACTAACCTGAACAACCTGATTAAAGGTACCGCGTTTGAAGGTAAATCACTGGAAGAGATTATTCg                                                                 <  2:546225/107‑1 (MQ=255)                                                                                                aCCATCGAGTATCACTACGGCAAGCACCATCAGACTTATGCCACTAACCTGAACAACCTGATTAAAGGTACCGCGTTTGAAGGTAAATCACTGGAAGAGATTATTCg                                                                 >  1:546225/1‑107 (MQ=255)                                                                                                aCCATCGAGTATCACTACGGCAAGCACCATCAGACTTATGCCACTAACCTGAACAACCTGATTAAAGGTACCGCGTTTGAAGGTAAATCACTGGAAGAGATTATTCGCAGCTCTGAAGGTGGCGTATTcaacaa                                      >  1:806404/1‑134 (MQ=255)                                                                                                aCCATCGAGTATCACTACGGCAAGCACCATCAGACTTATGCCACTAACCTGAACAACCTGATTAAAGGTACCGCGTTTGAAGGTAAATCACTGGAAGAGATTATTCGCAGCTCTGAAGGTGGCGTATTcaacaa                                      <  2:806404/134‑1 (MQ=255)                                                                                                aCCATCGAGTATCACTACGGCAAGCACCATCAGACTTATGCCACTAACCTGAACAACCTGATTAAAGGTACCGCGTTTGAAGGTAAATCACTGGAAGAGATTATTCGCAGCTCTGAAGGTGGCGTATTCAACAACGCAg                                 >  2:819714/1‑139 (MQ=255)                                                                                                  cATCGAGTATCACTACGGCAAGCACCATCAGACTTATGCCACTAACCTGAACAACCTGATTAAAGGTACCGCGTTTGAAGGTAAATCACTGGAAGAGATTATTCGCAGCTCTGAAGGTGGCGTATTCAACAACGCAGCt                               <  2:95086/139‑1 (MQ=255)                                                                                                  cATCGAGTATCACTACGGCAAGCACCATCAGACTTATGCCACTAACCTGAACAACCTGATTAAAGGTACCGCGTTTGAAGGTAAATCACTGGAAGAGATTATTCGCAGCTCTGAAGGTGGCGTATTCAACAACGCAGCt                               <  1:534639/139‑1 (MQ=255)                                                                                                                    cAAGCACCATCAGACTTATGCCACTAACCTGAACAACCTGATTAAAGGTACCGCGTTTGAAGGTAAATCACTGGAAGAGATTATTCGCAGCTCTGAAGGTGGCGTa                                              <  2:130401/106‑1 (MQ=255)                                                                                                                    cAAGCACCATCAGACTTATGCCACTAACCTGAACAACCTGATTAAAGGTACCGCGTTTGAAGGTAAATCACTGGAAGAGATTATTCGCAGCTCTGAAGGTGGCGTa                                              >  1:130401/1‑106 (MQ=255)                                                                                                                      aGCACCATCAGACTTATGCCACTAACCTGAACAACCTGATTAAAGGTACCGCGTTTGAAGGTAAATCACTGGAAGAGATTATTCGCAGCTCTGAAGGTGGCGTATTCAACAACg                                    <  1:128499/114‑1 (MQ=255)                                                                                                                      aGCACCATCAGACTTATGCCACTAACCTGAACAACCTGATTAAAGGTACCGCGTTTGAAGGTAAATCACTGGAAGAGATTATTCGCAGCTCTGAAGGTGGCGTATTCAACAACg                                    >  2:128499/1‑114 (MQ=255)                                                                                                                           cATCAGACTTATGCCACTAACCTGAACAACCTGATTAAAGGTACCGCGTTTGAAGGTAAATCAc                                                                                 <  2:669723/64‑1 (MQ=255)                                                                                                                           cATCAGACTTATGCCACTAACCTGAACAACCTGATTAAAGGTACCGCGTTTGAAGGTAAATCAc                                                                                 >  1:669723/1‑64 (MQ=255)                                                                                                                               aGACTTATGCCACTAACCTGAACAACCTGATTAAAGGTACCGCGTTTGAAGGTAAATCACTGGAAGAGATTATTCGCAGCTCTGAAGGTGGCGTATTCAACAACGCAGCTCAGGTCTGGAACCATACTTTCTACTGGaa  <  1:96293/139‑1 (MQ=255)                                                                                                                                     aTGCCACTAACCTGAACAACCTGATTAAAGGTACCGCGTTTGAAGGTAAATCACTGGAAGAGATTATTCGCa                                                               <  1:95459/72‑1 (MQ=255)                                                                                                                                        |                                                                                                                                  ATGCAAATTAATAATAAAGGAGAGTAGCAATGTCATTCGAATTACCTGCACTACCATATGCTAAAGATGCTCTGGCACCGCACATTTCTGCGGAAACCATCGAGTATCACTACGGCAAGCACCATCAGACTTATGTCACTAACCTGAACAACCTGATTAAAGGTACCGCGTTTGAAGGTAAATCACTGGAAGAGATTATTCGCAGCTCTGAAGGTGGCGTATTCAACAACGCAGCTCAGGTCTGGAACCATACTTTCTACTGGAA  >  NC_000913/1735349‑1735613

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑