Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,761,149 G→A H215Y (CAT→TAT)  sufD ← component of SufBCD Fe‑S cluster assembly scaffold

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,761,1490GA100.0% 84.8 / NA 27H215Y (CAT→TAT) sufDcomponent of SufBCD Fe‑S cluster assembly scaffold
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (13/14);  total (13/14)

CGTACTGGTGTTGTGTCGTAACACTGCGCCACCCAGCAGGAAACTGTGGCTAAATGCGGTGGCATCCTCAGCCAGCAACAAATCGTTATGAGCAAAGTGGTGACTGAGCGGGTTTTCAAACGCCAGCTTGATATGCTGCAAGTGGGCATTCGCTGCGACGTTGATAGTGAACCGTGCCCCGGTAAAATGACGAGCATCATTCAGGCTGACAAAATGTTCGATCACCGTTGCTTCGGCACCTTCCGCCAGATCCAGATGATGTCG  >  NC_000913/1761015‑1761278
                                                                                                                                      |                                                                                                                                 
cGTACTGGTGTTGTGTCGTAACACTGCGCCACCCAGCAGGAAACTGTGGCTAAATGCGGTGGCATCCTCAGCCAGCAACAAATCGTTATGAGCAAAGTGGTGACTGAGCGGGTTTTCAAACGCCAGCTTGATAtactgc                                                                                                                               >  2:269591/1‑139 (MQ=255)
       gtgtTGTGTCGTAACACTGCGCCACCCAGCAGGAAACTGTGGCTAAATGCGGTGGCACCCTCAGCCAGCAACAAATCGTTATGAGCAAAGTGGTGACTGAGCGGGTTTTCAAACGCCAGCTTGATATACTGCAAGTggg                                                                                                                        >  1:377127/1‑139 (MQ=255)
                 gTAACACTGCGCCACCCAGCAGGAAACTGTGGCTAAATGCGGTGGCATCCTCAGCCAGCAACAAATCGTTATGAGCAAAGTGGTGACTGAGCGGGTTTTCAAACGCCAGCTTGATATACTGCAAGTGGGCATTCGCTGc                                                                                                              >  2:26782/1‑139 (MQ=255)
                      acTGCGCCACCCAGCAGGAAACTGTGGCTAAATGCGGTGGCATCCTCAGCCAGCAACAAATCGTTATGAGCAAAGTGGTGACTGAGCGGGTTTTCAAACGCCAGCTTGATATACTGCAAGTGGGCATTCGCTGCGACGt                                                                                                         <  1:269591/139‑1 (MQ=255)
                         gcgcCACCCAGCAGGAAACTGTGGCTAAATGCGGTGGCATCCTCAGCCAGCAACAAATCGTTATGAGCAAAGTGGTGACTGAGCGGGTTTTCAAACGCCAGCTTGATATACTGCAAGTGGGCATTCGCTGCGACGTTGa                                                                                                      >  1:212857/1‑139 (MQ=255)
                                              tgGCTAAATGCGGTGGCATCCTCAGCCAGCAACAAATCGTTATGAGCAAAGTGGTGACTGAGCGGGTTTTCAAACGCCAGCTTGATATACTGCAAg                                                                                                                            >  2:999112/1‑96 (MQ=255)
                                              tgGCTAAATGCGGTGGCATCCTCAGCCAGCAACAAATCGTTATGAGCAAAGTGGTGACTGAGCGGGTTTTCAAACGCCAGCTTGATATACTGCAAg                                                                                                                            <  1:999112/96‑1 (MQ=255)
                                                    aaTGCGGTGGCATCCTCAGCCAGCAACAAATCGTTATGAGCAAAGTGGTGACTGAGCGGGTTTTCAAACGCCAGCTTGATATACTGCAAGTGGGCATTCGCTGCGACGTTGATAGTGAACCGTGCCCCGGTAAAATGAc                                                                           <  1:26782/139‑1 (MQ=255)
                                                        cGGTGGCATCCTCAGCCAGCAACAAATCGTTATGAGCAAAGTGGTGACTGAGCGGGTTTTCAAACGACAGCTTGATATACTGCAAGTGGGCATTCGCTGCGACGTTGATAGTGAACCGTGCCCCCGTAAAATGACGAGc                                                                       <  2:613744/139‑1 (MQ=255)
                                                           tGGCATCCTCAGCCAGCAACAAATCGTTATGAGCAAAGTGGTGACTGAGCGGGTTTTCAAACGCCAGCTTGATATACTGCAAGTGGGCATTCGCTGCGACGTTGATAGTGAACCGTGcccc                                                                                      >  2:78924/1‑121 (MQ=255)
                                                           tGGCATCCTCAGCCAGCAACAAATCGTTATGAGCAAAGTGGTGACTGAGCGGGTTTTCAAACGCCAGCTTGATATACTGCAAGTGGGCATTCGCTGCGACGTTGATAGTGAACCGTGcccc                                                                                      <  1:78924/121‑1 (MQ=255)
                                                             gCATCCTCAGCCAGCAACAAATCGTTATGAGCAAAGTGGTGACTGAGCGGGTTTTCAAACGCCAGCTTGATATACTGCAAGTGGGCATTCGCTGCGACGTTGATAGTGAACCGTGCCCCGGTAAAATGAc                                                                           <  2:402697/130‑1 (MQ=255)
                                                             gCATCCTCAGCCAGCAACAAATCGTTATGAGCAAAGTGGTGACTGAGCGGGTTTTCAAACGCCAGCTTGATATACTGCAAGTGGGCATTCGCTGCGACGTTGATAGTGAACCGTGCCCCGGTAAAATGAc                                                                           >  1:402697/1‑130 (MQ=255)
                                                                tCCTCAGCCAGCAACAAATCGTTATGAGCAAAGTGGTGACTGAGCGGGTTTTCAAACGCCAGCTTGATATACTGCAAGTGGGCATTCGCTGCGACGTTGATAGTGAACCGTGCCCCGGTAAAATGACGAGCATCATTCa                                                               >  1:745005/1‑139 (MQ=255)
                                                                tCCTCAGCCAGCAACAAATCGTTATGAGCAAAGTGGTGACTGAGCGGGTTTTCAAACGCCAGCTTGATATACTGCAAGTGGGCATTCGCTGCGACGTTGATAGTGAACCGTGCCCCGGTAAAATGACGAGCATCATTCa                                                               >  2:723488/1‑139 (MQ=255)
                                                                     agccagcAACAAATCGTTATGAGCAAAGTGGTGACTGAGCGGGTTTTCAAACGCCAGCTTGATATACTGCAAGTGGGCATTCGCTGCGACGTTGATAGTGAACCGTGCCCCGGTAAAATGACGAGCATCATTCAGGCTg                                                          >  1:433670/1‑139 (MQ=255)
                                                                       ccagcAACAAATCGTTATGAGCAAAGTGGTGACTGAGCGGGTTTTCAAACGCCAGCTTGATATACTGCAAGTGGGCATTCGCTGCGACGTTGATAGTGAACCGTGCCCCGGTAAAATGACGAGCATCATTCAGGCTGAc                                                        <  1:376757/139‑1 (MQ=255)
                                                                        cagcAACAAATCGTTATGAGCAAAGTGGTGACTGAGCGGGTTTTCAAACGCCAGCTTGATATACTGCAAGTGGGCATTCGCTGCGACGTTGATAGTGAACCGTGCCCCGGTAAAATGACGAGCATCATTCAGGCTGACa                                                       <  2:745005/139‑1 (MQ=255)
                                                                           caacaaATCGTTATGAGCAAAGTGGTGACTGAGCGGGTTTTCAAACGCCAGCTTGATATACTGCAAGTGGGCATTCGCTGCGACGTTGATAGTGAACCGTGCCCCGGTAAAATGACGAGCATCATTCAGGCTGACaaaa                                                    >  2:767707/1‑139 (MQ=255)
                                                                                           gCAAAGTGGTGACTGAGCGGGTTTTCAAACGCCAGCTTGATATACTGCAAGTGGGCATTCGCTGCGACGTTGATAGTGAACCGTg                                                                                          <  1:924272/85‑1 (MQ=255)
                                                                                           gCAAAGTGGTGACTGAGCGGGTTTTCAAACGCCAGCTTGATATACTGCAAGTGGGCATTCGCTGCGACGTTGATAGTGAACCGTg                                                                                          <  1:908802/85‑1 (MQ=255)
                                                                                           gCAAAGTGGTGACTGAGCGGGTTTTCAAACGCCAGCTTGATATACTGCAAGTGGGCATTCGCTGCGACGTTGATAGTGAACCGTg                                                                                          >  2:908802/1‑85 (MQ=255)
                                                                                           gCAAAGTGGTGACTGAGCGGGTTTTCAAACGCCAGCTTGATATACTGCAAGTGGGCATTCGCTGCGACGTTGATAGTGAACCGTg                                                                                          >  2:924272/1‑85 (MQ=255)
                                                                                                      aCTGAGTGGGTTTTCAAACGCCAGCTTGATATACTGCAAGTGGGCATTCGCTGCGACGTGGATAGTGAACCGTGCCCCGGTAAAATGACGAGCATCATTCAGGCTGACAAAATGTTCGAt                                            >  2:529518/1‑120 (MQ=255)
                                                                                                      aCTGAGCGGGTTTTCAAACGCCAGCTTGATATACTGCAAGTGGGCATTCGCTGCGACGTTGATAGTGAACCGTGCCCCGGTAAAATGACGAGCATCATTCAGGCTGACAAAATGTTCGAt                                            <  1:529518/120‑1 (MQ=255)
                                                                                                            cGGGTTTTCAAACGCCAGCTTGATATACTGCAAGTGGGCATTCGCTGCGACGTTGATAGTGAACCGTGCCCCGGTAAAATGACGAGCATCATTCAGGCTGACAAAATGTTCGATCACCGTTGCTTCGGCACCTTCCGcc                   <  2:377127/139‑1 (MQ=255)
                                                                                                                     aaaCGCCAGCTTGATATACTGCAAGTGGGCATTCGCTGCGACGTTGATAGTGAACCGTGCCCCGGTAAAATGACGAGCATCATTCAGGCTGACAAAATGTTCGATCACCGTTGCTTCGGCACCTTCCGccagatccaga          <  2:212857/139‑1 (MQ=255)
                                                                                                                             gCTTGATATACTGCAAGTGGGCATTCGCTGCGACGTTGATAGTGAACCGTGCCCCGGTAAAATGACGAGCATCATTCAGGCTGACAAAATGTTCGATCACCGTTGCTTCGGCACCTTCCGCCAGATCCAGATGATGTCg  <  1:723488/139‑1 (MQ=255)
                                                                                                                                      |                                                                                                                                 
CGTACTGGTGTTGTGTCGTAACACTGCGCCACCCAGCAGGAAACTGTGGCTAAATGCGGTGGCATCCTCAGCCAGCAACAAATCGTTATGAGCAAAGTGGTGACTGAGCGGGTTTTCAAACGCCAGCTTGATATGCTGCAAGTGGGCATTCGCTGCGACGTTGATAGTGAACCGTGCCCCGGTAAAATGACGAGCATCATTCAGGCTGACAAAATGTTCGATCACCGTTGCTTCGGCACCTTCCGCCAGATCCAGATGATGTCG  >  NC_000913/1761015‑1761278

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: