Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,905,805	G→A	G40S (GGC→AGC)	mntP →	putative Mn(2+) efflux pump, mntR‑regulated

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,905,805	0	G	A	100.0%	98.6 / NA	32	G40S (GGC→AGC)	mntP	putative Mn(2+) efflux pump, mntR‑regulated
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (13/19);  total (13/19)

GACATTGTCATGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGGTGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGTTTGTCCTTGAATGGAACCACTGGATTGCGTTTGTGC  >  NC_000913/1905679‑1905913                                                                                                                               |                                                                                                             gACATTGTCATGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGGTGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCAGCCTTATTTTTg                                                                                                  >  1:751139/1‑139 (MQ=255)  aCATTGTCATGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGGTGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCAGCCTTATTTTTgg                                                                                                 >  1:751792/1‑139 (MQ=255)    aTTGTCATGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATACATTTGCTGCATCAATCGGTAAAGGTGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCAGCCTTATTTTTGGTg                                                                                               <  2:129109/139‑1 (MQ=255)     ttGTCATGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTCGCTGCATCAATCGGTAAAGGTGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCAGCCTTATTTTTGGTGc                                                                                              <  1:740040/139‑1 (MQ=255)         cATGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATTCATTTGCTGCATCAATCGGTAAAGGTGCCACCATCCATAAACCGAAATTTTCTGAAGCATTGCGAACCAGCCTTATTTTTGGTGCCTTc                                                                                          <  1:779300/139‑1 (MQ=255)           tGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGGTGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCAGCCTTATTTTTGGTGCCGTCGa                                                                                        <  2:197626/139‑1 (MQ=255)                             ttcttcTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGGTGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCAGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGAt                                                                      <  2:751792/139‑1 (MQ=255)                              tcttctTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGGTGCCACCCTCCATAAACCGAAATTTTCTGAAACATTGCGAACCAGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATc                                                                     <  2:751139/139‑1 (MQ=255)                                 tcttGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGGTGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCAGCCTTATTTTTGGTg                                                                                               <  2:641242/110‑1 (MQ=255)                                 tcttGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGGTGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCAGCCTTATTTTTGGTg                                                                                               >  1:641242/1‑110 (MQ=255)                                         ttGGTATGTCGATGGATGCATTTGCTCCATCAATCGGTAAAGGTGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCAGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGGGAAt                                                          <  2:42498/139‑1 (MQ=255)                                          tGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGGTGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCAGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGGGAATg                                                         >  1:494261/1‑139 (MQ=255)                                                      ggatgCATTTGCTGCATCAATCGGTAAAGGTGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCAGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGGGAATGGGCATGTTagcc                                             >  1:936418/1‑139 (MQ=255)                                                         tgCATTTGCTGCATCAATCGGTAAAGGTGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCAGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGGGAATGGGCATGTTagccagc                                          >  2:320171/1‑139 (MQ=255)                                                             tttGCTGCATCAATCGGTAAAGGTGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCAGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGt                                      <  1:320171/139‑1 (MQ=255)                                                                        aaTCGGTAAAGGTGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCAGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGTTTGTCCTTGaa                           <  1:194626/139‑1 (MQ=255)                                                                           cGGTAAAGGTGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCAGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGTTTGTCCTTGAATgg                        <  2:936418/139‑1 (MQ=255)                                                                                       cACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCAGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGAt                                                                      >  1:880319/1‑81 (MQ=255)                                                                                       cACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCAGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGAt                                                                      <  2:880319/81‑1 (MQ=255)                                                                                            tCCATAAACCGAAATTTTCTGAAGCATTGCGAACCAGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGTTTGTCCTTGAATGGAACCACTGGATTGCGtt       <  2:494261/139‑1 (MQ=255)                                                                                              cATAAACCGAAATTTTCTGAAGCATTGCGAACCAGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGTTTGTCCt                               >  2:896384/1‑113 (MQ=255)                                                                                              cATAAACCGAAATTTTCTGAAGCATTGCGAACCAGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGTTTGTCCt                               <  1:896384/113‑1 (MQ=255)                                                                                                       aaaTTTTCTGAAGCATTGCGAACCAGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGTTTGTCCTTGAATgg                        >  2:429340/1‑111 (MQ=255)                                                                                                       aaaTTTTCTGAAGCATTGCGAACCAGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGTTTGTCCTTGAATgg                        <  1:429340/111‑1 (MQ=255)                                                                                                        aaTTTTCTGAAGCATTGCGAACCAGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGGGa                                                            >  2:499962/1‑74 (MQ=255)                                                                                                        aaTTTTCTGAAGCATTGCGAACCAGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGGGa                                                            <  1:499962/74‑1 (MQ=255)                                                                                                        aaTTTTCTGAAGCATTGCGAACCAGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGTTTGTCCTTGAATGGAACCACTGGATTGCGt        >  1:589941/1‑126 (MQ=255)                                                                                                        aaTTTTCTGAAGCATTGCGAACCAGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGTTTGTCCTTGAATGGAACCACTGGATTGCGt        <  2:589941/126‑1 (MQ=255)                                                                                                        aaTTTTCTGAAGCATTGCGAACCAGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGTTTGTCCTTGAATGGAACCACTGGATTGCGTTTGtgc  <  2:796791/132‑1 (MQ=255)                                                                                                        aaTTTTCTGAAGCATTGCGAACCAGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGTTTGTCCTTGAATGGAACCACTGGATTGCGTTTGtgc  >  1:796791/1‑132 (MQ=255)                                                                                                                    cATTGCGAACCAGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGTTTGTcc                                >  2:725843/1‑90 (MQ=255)                                                                                                                    cATTGCGAACCAGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGTTTGTcc                                <  1:725843/90‑1 (MQ=255)                                                                                                                               |                                                                                                             GACATTGTCATGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGGTGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGTTTGTCCTTGAATGGAACCACTGGATTGCGTTTGTGC  >  NC_000913/1905679‑1905913

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑