Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,016,095	G→A	L76L (CTG→CTA)	fliH →	negative regulator of FliI ATPase activity

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,016,095	0	G	A	100.0%	118.6 / NA	37	L76L (CTG→CTA)	fliH	negative regulator of FliI ATPase activity
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (19/18);  total (19/18)

GAGGCTGAACCCAGCCTTGAGCAGCAACTGGCGCAACTGCAAATGCAGGCCCATGAGCAAGGTTATCAGGCGGGTATTGCCGAAGGTCGCCAGCAAGGTCATAAGCAGGGCTATCAGGAAGGACTGGCCCAGGGGCTGGAGCAAGGTCTGGCAGAGGCGAAGTCTCAACAAGCGCCAATTCATGCCCGGATGCAGCAACTGGTCAGCGAATTTCAAACTACCCTTGATGCACTTGATAGTGTGAT  >  NC_000913/2015970‑2016214                                                                                                                              |                                                                                                                        gagGCTGAACCCAGCCTTGAGCAGCAACTGGCGCAACTGCAAATGCAGGCCCATGAGCAAGGTTATCAGGCGGGTATTGCCGAAGGTCGCCAGCAAGGTCATAAGCAGGGCTATCAGGAAGGACTAGCCCAGGGGCTgg                                                                                                            >  1:1003963/1‑139 (MQ=255)   ggCTGAACCCAGCCTTGAGCAGCAACTGGCGCAACTGCAAATGCAGGCCCATGAGCAAGGTTATCAGGCGGGTATTGCCGAAGGTCGCCAGCAAGGTCATAAGCAGGGCTATCAGGAAGGACTAGCCCAGGGGCTGGAg                                                                                                          >  1:92837/1‑139 (MQ=255)    gCTGAACCCAGCCTTGAGCAGCAACTGGCGCAACTGCAAATGCAGGCCCATGAGCAAGGTTATCAGGCGGGTATTGCCGAAGGTCGCCAGCAAGGTCATAAGCAGGGCTATCAGGAAGGACTAGCCCAGGGGCTGGAGc                                                                                                         >  2:884357/1‑139 (MQ=255)     cTGAACCCAGCCTTGAGCATCAACTGGCGCAACTGCAAATGCAGGCCCATGAGCAAGGTTATCAGGCGGGTATTGCCGAAGGTCGCCAGCAAGGTCATAAGCAGGGCTATCAGGAAGGACTAGCCCAGGGGCTGGAGCa                                                                                                        <  2:174625/139‑1 (MQ=255)        aaCCCAGCCTTGAGCAGCAACTGGCGCAACTGCAAATGCAGGCCCATGAGCAAGGTTATCAGGCGGGTATTGCCGAAGGTCGCCAGCAAGGTCATAAGCAGGGCTATCAGGAAGGACTAGCCCAGGGGCTGGAGCAAgg                                                                                                     <  2:599689/139‑1 (MQ=255)         aCCCAGCCTTGAGCAGCAACTAGCGCAAGTGCAAATGCAGGCCCATGAGCAAGGTTATCAGGCGGGTATTGCCGAAGGACGCCAGCAAGGTCATAAGCAGGGCTATCAGGAAGGACTAGCCCAGGGGCTGGAGCAAGGt                                                                                                    >  2:764688/1‑139 (MQ=255)                  tGAGCAGCAACTGGCGCAACTGCAAATGCAGGCCCATGAGCAAGGTTATCAGGCGGGTATTGCCGAAGGTCGCCAGCAAGGTCATAAGCAGGGCTATCAGGAAGGACTAGCCCAGGGGCTGGAGCAAGGTCTGGCagag                                                                                           >  2:818188/1‑139 (MQ=255)                       agcaACTGGCGCAACTGCAAATGCAGGCCCATGAGCAAGGTTATCAGGCGGGTATTGCCGAAGGTCGCCAGCAAGGTCATAAGCAGGGCTATCAGGAAGGACTAGCCCAGGGGCTGGAGCAAGGTCTGGCAGAGGCGaa                                                                                      >  2:542361/1‑139 (MQ=255)                                   aaCTGCAAATGCAGGCCCATGAGCAAGGTTATCAGGCGGGTATTGCCGAAGGTCGCCAGCAAGGTCATAAGCAGGGCTATCAGGAAGGACTAGCCCAGGGGCTGGAGCAAGGTCTGGCAGAGGCGAAGTCTCAACAAgc                                                                          >  2:84505/1‑139 (MQ=255)                                     cTGCAAATGCAGGCCCATGAGCAAGGTTATCAGGCGGGTATTGCCGAAGGTCGCCAGCAAGGTCATAAGCAGGGCTATCAGGAAGGACTAGCCCAGGGGCTGGAGCAAGGTCTGGCAGAGGCGAAGTCTCAACAAgcgc                                                                        <  2:1003963/139‑1 (MQ=255)                                     cTGCAAATGCAGGCCCATGAGCAAGGTTATCAGGCGGGTATTGCCGAAGGTCGCCAGCAAGGTCATAAGCAGGGCTATCAGGAAGGACTAGCCCAGGGGCTGGAGCAAGGTCTGGCAGAGGCGAAGTCTCAACAAgcgc                                                                        <  1:457209/139‑1 (MQ=255)                                      tGCAAATGCAGGCCCATGAGCAAGGTTATCAGGCGGGTATTGCCGAAGGTCGCCAGCAAGGTCATAAGCAGGGCTATCAGGAAGGACTAGCCCAgggg                                                                                                                <  1:663608/98‑1 (MQ=255)                                      tGCAAATGCAGGCCCATGAGCAAGGTTATCAGGCGGGTATTGCCGAAGGTCGCCAGCAAGGTCATAAGCAGGGCTATCAGGAAGGACTAGCCCAgggg                                                                                                                >  2:663608/1‑98 (MQ=255)                                                ggCCCATGAGCAAGGTTATCAGGCGGGTATTGCCGAAGGTCGCCAGCAAGGTCATAAGCAGGGCTATCAGGAAGGACTAGCCCAGGGGCTGGAGCAAGGTCTGGCAGAGGCg                                                                                        <  2:330775/112‑1 (MQ=255)                                                ggCCCATGAGCAAGGTTATCAGGCGGGTATTGCCGAAGGTCGCCAGCAAGGTCATAAGCAGGGCTATCAGGAAGGACTAGCCCAGGGGCTGGAGCAAGGTCTGGCAGAGGCg                                                                                        >  1:330775/1‑112 (MQ=255)                                                             ggTTATCAGGCGGGTATTGCCGAAGGTCGCCAGCAAGGTCATAAGCAGGGCTATCAGGAAGGACTAGCCCAGGGGCTGGAGCAAGGTCTGGCAGAGGCGAAGTCTCAACAAGCGCCAATTCATGCCCGGATGCAGCAAc                                                >  2:528893/1‑139 (MQ=255)                                                             ggTTATCAGGCGGGTATTGCCGAAGGTCGCCAGCAAGGTCATAAGCAGGGCTATCAGGAAGGACTAGCCCAGGGGCTGGAGCAAGGTCTGGCAGAGGCGAAGTCTCAACAAGCGCCAATTCATGCCCGGATGCAGCAAc                                                >  1:945895/1‑139 (MQ=255)                                                                tATCAGGCGGGTATTGCCGAAGGTCGCCAGCAAGGTCATAAGCAGGGCTATCAGGAAGGACTAGCCCAGGGGCTGGAGCAAGGTCTGGCAGAGGCGAAGTCTCAACAAGCGCCAATTCATGCCCGGATGCAGCAACTgg                                             >  2:240281/1‑139 (MQ=255)                                                                 aTCAGGCGGGTATTGCCGAAGGTCGCCAGCAAGGTCATAAGCAGGGCTATCAGGAAGGACTAGCCCAGGGGCTGGAGCAAGGTCTGGCAGAGGCGAAGTCTCAACAAGCGCCAATTCATGCCCGGATGCAGCAACTGGt                                            <  2:92837/139‑1 (MQ=255)                                                                                        cGCCAGCAAGGTCATAAGCAGGGCTATCAGGAAGGACTAGCCCAGGGGCTGGAGCAAGGTCTGGCAGAGGCGAAGTCTCAACAAGCGCCAATTCATGCCCGGATGCAGCAACTGGTCAGCGAATTTCAAACTACCCTTg                     <  2:945895/139‑1 (MQ=255)                                                                                        cGCCAGCAAGGTCATAAGCAGGGCTATCAGGAAGGACTAGCCCAGGGGCTGGAGCAAGGTCTGGCAGAGGCGAAGTCTCAACAAGCGCCAATTCATGCCCGGATGCAGCAACTGGTCAGCGAATTTCAAACTACCCTTg                     <  1:251644/139‑1 (MQ=255)                                                                                         gCCAGCAAGGTCATAAGCAGGGCTATCAGGAAGGACTAGCCCAGGGGCTGGAGCAAGGTCTGGCAGAGGCGAAGTCTCAACAAGCGCCAATTCATGCCCGGATGCAGCAACTGGTCAGCGAATTTCAAACTACCCTTGa                    >  2:866608/1‑139 (MQ=255)                                                                                                   tCATAAGCAGGGCTATCAGGAAGGACTAGCCCAGGGGCTGGAGCAAGGTCTGGCAGAGGCGAAGTCTCAACAAGCGCCAATTCATGCCCGGATGCAGCAACTGGTCAGCGAATTTCAAACTACCCTTGATGCACTTGAt          <  1:884357/139‑1 (MQ=255)                                                                                                   tCATAAGCAGGGCTATCAGGAAGGACTAGCCCAGGGGCTGGAGCAAGGACTGGCAGAGGCGAAGTCTCAACAAGCGCCAATGCATGCCCGGATGCAGCAACTGGTCAGCGAATTTCAAACTACCCTTGATGCACTTGAt          <  1:764688/139‑1 (MQ=255)                                                                                                           aGGGCTATCAGGAAGGACTAGCCCAGGGGCTGGAGCAAGGTCTGGCAGAGGCGAAGTCTCAACAAGCGCCAATTCATGCCCGGATGCAGCAACTGGTCAGCGAATTTCAAACTACCCTTGATGCACTTGATAGTGTGAt  <  1:542361/139‑1 (MQ=255)                                                                                                             ggCTATCAGGAAGGACTAGCCCAGGGGCTGGAGCAAGGTCTGGCAGAGGCGAAGTCTCAACAAGCGCCAATTCATGCCCGGATGCAGCAACTGGTCAGCGAATTTCAAACTACCCTTg                     <  1:104350/118‑1 (MQ=255)                                                                                                             ggCTATCAGGAAGGACTAGCCCAGGGGCTGGAGCAAGGTCTGGCAGAGGCGAAGTCTCAACAAGCGCCAATTCATGCCCGGATGCAGCAACTGGTCAGCGAATTTCAAACTACCCTTg                     >  2:104350/1‑118 (MQ=255)                                                                                                             ggCTATCAGGAAGGACTAGCCCAGGGGCTGGAGCAAGGTCTGGCAGAGGCGAAGTCTCAACAAGCGCCAATTCATGCCCGGATGCAGCAACTGGTCAGCGAATTTCAAACTACCCTTGAt                   >  2:617852/1‑120 (MQ=255)                                                                                                             ggCTATCAGGAAGGACTAGCCCAGGGGCTGGAGCAAGGTCTGGCAGAGGCGAAGTCTCAACAAGCGCCAATTCATGCCCGGATGCAGCAACTGGTCAGCGAATTTCAAACTACCCTTGAt                   <  1:617852/120‑1 (MQ=255)                                                                                                             ggCTATCAGGAAGGACTAGCCCAGGGGCTGGAGCAAGGTCTGGCAGAGGCGAAGTCTCAACAAGCGCCAATTCATGCCCGGATGCAGCAACTGGTCAGCGAATTTCAAACTACCCTTGATGc                 >  2:188735/1‑122 (MQ=255)                                                                                                             ggCTATCAGGAAGGACTAGCCCAGGGGCTGGAGCAAGGTCTGGCAGAGGCGAAGTCTCAACAAGCGCCAATTCATGCCCGGATGCAGCAACTGGTCAGCGAATTTCAAACTACCCTTGATGc                 <  1:188735/122‑1 (MQ=255)                                                                                                                  tCAGGAAGGACTAGCCCAGGGGCTGGAGCAAGGTCTGGCAGAGGCGAAGTCTCAACAAGCGCCAATTCATGCCCGGATGCAGCAACTGGTCAGCGAATTTCAAACTACCCTTGATg                  <  2:159975/116‑1 (MQ=255)                                                                                                                  tCAGGAAGGACTAGCCCAGGGGCTGGAGCAAGGTCTGGCAGAGGCGAAGTCTCAACAAGCGCCAATTCATGCCCGGATGCAGCAACTGGTCAGCGAATTTCAAACTACCCTTGATg                  >  1:159975/1‑116 (MQ=255)                                                                                                                   cAGGAAGGACTAGCCCAGGGGCTGGAGCAAGGTCTGGCAGAGGCGAAGTCTCAACAAGCGCCAATTCATGCCCGGATGCAGCAACTGGTCAGCGAATTTCAAACTACCCTTg                     >  2:22550/1‑112 (MQ=255)                                                                                                                   cAGGAAGGACTAGCCCAGGGGCTGGAGCAAGGTCTGGCAGAGGCGAAGTCTCAACAAGCGCCAATTCATGCCCGGATGCAGCAACTGGTCAGCGAATTTCAAACTACCCTTg                     <  1:22550/112‑1 (MQ=255)                                                                                                                        aggaCTAGCCCAGGGGCTGGAGCAAGGTCTGGCAGAGGCGAAGTCTCAACAAGCGCCAAtt                                                                   >  1:933265/1‑61 (MQ=255)                                                                                                                        aggaCTAGCCCAGGGGCTGGAGCAAGGTCTGGCAGAGGCGAAGTCTCAACAAGCGCCAAtt                                                                   <  2:933265/61‑1 (MQ=255)                                                                                                                              |                                                                                                                        GAGGCTGAACCCAGCCTTGAGCAGCAACTGGCGCAACTGCAAATGCAGGCCCATGAGCAAGGTTATCAGGCGGGTATTGCCGAAGGTCGCCAGCAAGGTCATAAGCAGGGCTATCAGGAAGGACTGGCCCAGGGGCTGGAGCAAGGTCTGGCAGAGGCGAAGTCTCAACAAGCGCCAATTCATGCCCGGATGCAGCAACTGGTCAGCGAATTTCAAACTACCCTTGATGCACTTGATAGTGTGAT  >  NC_000913/2015970‑2016214

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑