Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,053,940	A→G	T100A (ACC→GCC)	shiA →	shikimate transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,053,940	0	A	G	100.0%	74.5 / NA	22	T100A (ACC→GCC)	shiA	shikimate transporter
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (10/12);  total (10/12)

GCAAGTAAGCCCGGCGATGGGAACGCTCGCCGCATTTGCTACCTTTGGCGTCGGATTTCTTTTCCGTCCGCTCGGCGGTGTCATTTTCGGTCACTTTGGCGACCGACTGGGACGTAAGCGCATGTTAATGCTGACCGTCTGGATGATGGGCATCGCGACAGCCTTGATTGGTATTCTTCCTTCATTCTCGACCATTGGGTGGTGGGCACCTATTTTGCTGGTGACACTGCGTG  >  NC_000913/2053807‑2054039                                                                                                                                      |                                                                                                    gCAAGTAAGCCCGGCGATGGGAACGCTCGCCGCATTTGCTACCTTTGGCGTCGGATTTCTTTTCCGTCCGCTCGGCGGTGTCATTTTCGGTCACTTTGGCGACCGACTGGGACGTAAGCGCATGTTAATGCTGGCCGTc                                                                                                <  2:688551/139‑1 (MQ=255)        aGCCCGGCGATGGGAACGCTCGCCGCATTTGCTACCTTTGGCGTCGGATTTCTTTTCCGTCCGCTCGGCGGTGTCATTTTCGGTCACTTTGGCGACCGACTGGGACGTAAGCGCATGTTAATGCTGGCCGTCTGgatga                                                                                         <  2:811275/139‑1 (MQ=255)                       aCGCTCGCCGCATTTGCTACCTTTGGCGTCGGATTTCTTTTCCGTCCGCTCGGCGGTGTCATTTTCGGTCACTTTGGCGACCGACTGGGACGTAAGCGCATGTTAATGCTGGCCGTCTGGATGATGGGCATCGCGACAg                                                                          <  2:416258/139‑1 (MQ=255)                                           cTTTGGCGTCGGATTTCTTTTCCGTCCGCTCGGCGGTGTCATTTTCGGTCACTTTGGCGACCGACTGGGACGTAAGCGCATGTTAATGCTGGCCGTCTGGATGATGGGCATCGCGACAGCCTTGATTGGTATTcttcct                                                      >  2:229779/1‑139 (MQ=255)                                                gCGTCGGATTTCTTTTCCGTCCGCTCGGCGGTGTCATTTTCGGTCACTTTGGCGACCGACTGGGACGTAAGCGCATGTTAATGCTGGCCGTCTGGATGATGGGCATCGCGACAGTCTTGAt                                                                   <  1:66161/121‑1 (MQ=255)                                                gCGTCGGATTTCTTTTCCGTCCGCTCGGCGGTGTCATTTTCGGTCACTTTGGCGACCGACTGGGACGTAAGCGCATGTTAATGCTGGCCGTCTGGATGATGGGCATCGCGACAGTCTTGAt                                                                   >  2:66161/1‑121 (MQ=255)                                                                ccgtccgCTCGGCGGTGTCATTTTCGGTCACTTTGGCGACCGACTGGGACGTAAGCGCATGTTAATGCTGGCCGTCTGGATGATGGGCATCGCGACAGCCTTGATTGGTATTCTTCCTTCATTCTCGACCATTGggtgg                                 >  2:251214/1‑139 (MQ=255)                                                                 cgtccgCTCGGCGGTGTCATTTTCGGTCACTTTGGCGACCGACTGGGACGTAAGCGCATGTTAATGCTGGCCGTCTGGATGATGGGCATCGCGACAGCCTTGATTGGTATTCTTCCTTCATTCTCGACCATTGggtggt                                >  1:356895/1‑139 (MQ=255)                                                                   tccgCTCAGCGGTGTCATTTTCGGTCACTTTGGCGCCCGACTGGGACGTAAGCGCATGTTAATGCTGGCCGTCTGGATGATGGGCATCGCGACAGCCTTGATTGGTATTCTTCCTTCATTCTCGACCATTAggtggtag                              >  1:436353/1‑137 (MQ=255)                                                                    ccgCTCGGCGGTGTCATTTTCGGTCACTTTGGCGACCGACTGGGACGTAAGCGCATGTTAATGCTGGCCGTCTGGATGATGGGCATCGCGACAGCCTTGATTGGTATTCTTCCTTCATTCTCGACCATTGGGTGGTggg                             <  2:799494/139‑1 (MQ=255)                                                                               tgtCATTTTCGGTCACTTTGGCGACCGACTGGGACGTAAGCGCATGTTAATGCTGGCCGTCTGGATGATGGGCATCGCGACAGCCTTGATTGGTa                                                              >  1:24392/1‑95 (MQ=255)                                                                               tgtCATTTTCGGTCACTTTGGCGACCGACTGGGACGTAAGCGCATGTTAATGCTGGCCGTCTGGATGATGGGCATCGCGACAGCCTTGATTGGTa                                                              <  2:24392/95‑1 (MQ=255)                                                                                gtCATTTTCGGTCACTTTGGCGACCGACTGGGACGTAAGCGCATGTTAATGCTGGCCGTCTGGATGATGGGCATCGCGACAGCCTTGATTGGTATTCTTCCTTCATTCTCGACCATTGGGTGGTGGGCACCTATTTTGc                 >  1:96906/1‑139 (MQ=255)                                                                                     tttCGGTCACTTTGGCGACCGACTGGGACGTAAGCGCATGTTAATGCTGGCCGTCTGGATGATGGGCATCGCGACAGCCTTGATTGGTATTCTTCCTTCATTCTCGACCATTGGGTGGTGGGCACCTATTTTGCTGGTg            <  1:313610/139‑1 (MQ=255)                                                                                        cGGTCACTTTGGCGACCGACTGGGACGTAAGCGCATGTTAATGCTGGCCGTCTGGATGATGGGCATCGCGACAGCCTTGATTGGTATTCTTCCTTCATTCTCGACCATTGGGTGGTGGGCACCTATTTTGCTGGTGaca         <  2:96906/139‑1 (MQ=255)                                                                                               tttGGCGACCGACTGGGACGTAAGCGCATGTTAATGCTGGCCGTCTGGATGATGGGCATCGCGACAGCCTTGATTGGTATTCTTCCTTCATTCTCGACCATTGGGTGGTGGGCACCTATTTTGCTGGTGACACTGCGTg  <  1:229779/139‑1 (MQ=255)                                                                                                         gacTGGGACGTAAGCGCATGTTAATGCTGGCCGTCTGGatgatg                                                                                       >  2:317808/1‑44 (MQ=255)                                                                                                         gacTGGGACGTAAGCGCATGTTAATGCTGGCCGTCTGGatgatg                                                                                       <  1:317808/44‑1 (MQ=255)                                                                                                         gacTGGGACGTAAGCGCATGTTAATGCTGGCCGTCTGGATGATGGGCATcgcg                                                                              >  1:852219/1‑53 (MQ=255)                                                                                                         gacTGGGACGTAAGCGCATGTTAATGCTGGCCGTCTGGATGATGGGCATcgcg                                                                              <  2:852219/53‑1 (MQ=255)                                                                                                                     aGCGCATGTTAATGCTGGCCGTCTGGATGATGGGCATCGCGAAAGCCTTGATTGGTATTCTTCCTTCATTCTCGACCAtt                                       <  2:675669/80‑1 (MQ=255)                                                                                                                     aGCGCATGTTAATGCCGGCCGTCTGGATGATGGGCATCGCGACAGCCTTGATTGGTATTCTTCCTTCATTCTCGACCAtt                                       >  1:675669/1‑80 (MQ=255)                                                                                                                                      |                                                                                                    GCAAGTAAGCCCGGCGATGGGAACGCTCGCCGCATTTGCTACCTTTGGCGTCGGATTTCTTTTCCGTCCGCTCGGCGGTGTCATTTTCGGTCACTTTGGCGACCGACTGGGACGTAAGCGCATGTTAATGCTGACCGTCTGGATGATGGGCATCGCGACAGCCTTGATTGGTATTCTTCCTTCATTCTCGACCATTGGGTGGTGGGCACCTATTTTGCTGGTGACACTGCGTG  >  NC_000913/2053807‑2054039

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑