Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,318,019	G→A	R620C (CGT→TGT)	rcsC ←	hybrid sensory kinase in two‑component regulatory system with RcsB and YojN

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,318,019	0	G	A	100.0%	133.7 / NA	42	R620C (CGT→TGT)	rcsC	hybrid sensory kinase in two‑component regulatory system with RcsB and YojN
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (21/21);  total (21/21)

TCAGTTTTTCACAAATCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTCAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCC  >  NC_000913/2317884‑2318146                                                                                                                                        |                                                                                                                                tCAGTTTTTCACAAATCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACAGAt                                                                                                                              <  2:209881/139‑1 (MQ=255)          cacaAATCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACAGATAGAGAGATa                                                                                                                     >  1:494181/1‑139 (MQ=255)                tCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACAGATAGAGAGATAATCGcc                                                                                                               <  2:494181/139‑1 (MQ=255)                  gCCAGACCCAGACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACAGATAGAGAGATAATCGCCAt                                                                                                             >  1:531356/1‑139 (MQ=255)                               tggTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACAGATAGAGAGATAAACGCCATCCGCGCGAACATg                                                                                                <  1:426014/138‑1 (MQ=255)                               cGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCTAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACAGATAGAGAGATAATCGCCATCCGCGCGAACATg                                                                                                <  2:669001/139‑1 (MQ=255)                                 gTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACAGATAGAGAGATAATCGcc                                                                                                               <  2:363634/122‑1 (MQ=255)                                 gTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACAGATAGAGAGATAATCGcc                                                                                                               >  1:363634/1‑122 (MQ=255)                                 gTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACAGATAGAGAGATAATCGCCATCCGCGCGa                                                                                                     >  1:927085/1‑132 (MQ=255)                                 gTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACAGATAGAGAGATAATCGCCATCCGCGCGa                                                                                                     <  2:927085/132‑1 (MQ=255)                                 gTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACAGATAGAGAGATAATCGCCATCCGCGCGAACATGCa                                                                                              <  1:143044/139‑1 (MQ=255)                                  tCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACAGATAGAGAGATAATCGCCATCcgcg                                                                                                        >  1:301004/1‑128 (MQ=255)                                  tCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACAGATAGAGAGATAATCGCCATCcgcg                                                                                                        <  2:301004/128‑1 (MQ=255)                                       tGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACAGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACta                                                                                        >  2:228392/1‑139 (MQ=255)                                            aTTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACAGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAg                                                                                   >  2:872270/1‑139 (MQ=255)                                              tACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACAGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGcc                                                                                 >  2:975301/1‑139 (MQ=255)                                                     tACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACAGATAgagaga                                                                                                                       <  2:44914/94‑1 (MQ=255)                                                     tACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACAGATAgagaga                                                                                                                       >  1:44914/1‑94 (MQ=255)                                                             ttCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACAGATaga                                                                                                                           >  1:673693/1‑82 (MQ=255)                                                             ttCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACAGATaga                                                                                                                           <  2:673693/82‑1 (MQ=255)                                                             ttCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACAGATAGAGAGATAATCGCCATCCGCGCGa                                                                                                     >  2:931861/1‑104 (MQ=255)                                                             ttCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACAGATAGAGAGATAATCGCCATCCGCGCGa                                                                                                     <  1:931861/104‑1 (MQ=255)                                                                                 tCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACAGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAAc                                                                                          >  1:69348/1‑95 (MQ=255)                                                                                 tCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACAGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAAc                                                                                          <  2:69348/95‑1 (MQ=255)                                                                                       aaGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACAGa                                                                                                                               >  1:711813/1‑52 (MQ=255)                                                                                       aaGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACAGa                                                                                                                               <  2:711813/52‑1 (MQ=255)                                                                                        aGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACAGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAAATATACAGCCGGTATCGGTGAATTTTATGGCGTTACTCAACAGGTTGGAGAt                                       >  2:865028/1‑139 (MQ=255)                                                                                          cgcACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACAGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACtata                                                                                      >  1:380889/1‑90 (MQ=255)                                                                                          cgcACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACAGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACtata                                                                                      <  2:380889/90‑1 (MQ=255)                                                                                          cgcACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACAGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTCaa                                                   >  1:765111/1‑125 (MQ=255)                                                                                          cgcACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACAGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTCaa                                                   <  2:765111/125‑1 (MQ=255)                                                                                          cgcACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACAGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTCAACAGGt                                              >  1:847754/1‑130 (MQ=255)                                                                                          cgcACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACAGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTCAACAGGt                                              <  2:847754/130‑1 (MQ=255)                                                                                             accacTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACAGATAGAGAGATAATCGCCATCCGCGCGAACATGCaaaa                                                                                           >  1:703091/1‑82 (MQ=255)                                                                                             accacTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACAGATAGAGAGATAATCGCCATCCGCGCGAACATGCaaaa                                                                                           <  2:703091/82‑1 (MQ=255)                                                                                                   tcttTCGCCGGTATCCCCACGCCGGTATCGCGAACACAGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTCAACAGGTTGGAGATGACCTGCTGTa                            <  1:153233/139‑1 (MQ=255)                                                                                                                  cccACGCCGGTATCGCGAACACAGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTCAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGc             >  1:884709/1‑139 (MQ=255)                                                                                                                  cccACGCCGGTATCGCGAACACAGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTCAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGc             <  1:228392/139‑1 (MQ=255)                                                                                                                   ccACGCCGGTATCGCGAACACAGATAGAGAGATAATCGCCATCCGCGCGAACATGCaaaa                                                                                           <  1:983284/60‑1 (MQ=255)                                                                                                                   ccACGCCGGTATCGCGAACACAGATAGAGAGATAATCGCCATCCGCGCGAACATGCaaaa                                                                                           >  2:983284/1‑60 (MQ=255)                                                                                                                           gTATCGCGAACACAGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTCAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAgg    >  1:637588/1‑139 (MQ=255)                                                                                                                             aTCGCGAACACAGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTCAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGcc  <  2:884709/139‑1 (MQ=255)                                                                                                                                        |                                                                                                                                TCAGTTTTTCACAAATCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTCAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCC  >  NC_000913/2317884‑2318146

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑