Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,449,228	C→T	*274* (TAG→TAA)	yfcO ←	DUF2544 family putative outer membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,449,228	0	C	T	100.0%	67.2 / NA	22	*274* (TAG→TAA)	yfcO	DUF2544 family putative outer membrane protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (9/13);  total (9/13)

CGTTGTTGGTGTTGATTGAAGTCGAAGAGTGGCTGCCGCCGGAGTTGCCCTGAGGAGTTGAGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTC  >  NC_000913/2449105‑2449362                                                                                                                            |                                                                                                                                       cgTTGTTGGTGGTGATTGAAGTCGAAGAGTGGCTGCCGCCGGAGTTGCCCTGAGAAGTTGAGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGGCTGGTGTATCTAAGGGTTACAACTCATTCAGt                                                                                                                         <  2:703180/139‑1 (MQ=255)     gttgGTGTTGATTGAAGTCGAAGAGTGGCTGCCGCCGGAGTTGCCCTGAGGAGTTGAGCGCGTTGCGCCAGGTACGGTTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGTTACAACTCATTCAGTATGt                                                                                                                     >  1:640599/1‑139 (MQ=255)       tgGTGTTGATTGAAGTCGAAGAGTGGCTGCCGCCGGAGTTGCCCTGAGGAGTTGAGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGTTACAACTCATTCAGTATGTAc                                                                                                                   <  2:420464/139‑1 (MQ=255)                    aGTCGAAGAGTGGCTGCCGCCGGAGTTGCCCTGAGGAGTTGAGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGTTACa                                                                                                                                    <  2:718390/109‑1 (MQ=255)                    aGTCGAAGAGTGGCTGCCGCCGGAGTTGCCCTGAGGAGTTGAGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGTTACa                                                                                                                                    >  1:718390/1‑109 (MQ=255)                                gCTGCCGCCGGAGTTGCCCTGAGGAGTTGAGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGTTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTg                                                                                          >  1:322207/1‑139 (MQ=255)                                 cTGCCGCCGGAGTTGCCCTGAGGAGTTGAGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGTTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTgg                                                                                         <  1:428215/139‑1 (MQ=255)                                                cccTGAGGAGTTGAGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGTTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGt                                                                          >  2:992619/1‑139 (MQ=255)                                                       gagTTGAGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGTTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAaca                                                                   <  2:97502/139‑1 (MQ=255)                                                                                 ggTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGTTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGttt                                         <  1:535285/139‑1 (MQ=255)                                                                                   tAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGTTACAACTCCTTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGt                                       <  2:640599/139‑1 (MQ=255)                                                                                   tAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGTTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGt                                       <  1:992619/139‑1 (MQ=255)                                                                                            gggCGAATGCCCCTGCCTGGTGTATCTAAGGGTTACAACTCATTCAGTATGTACTGGAACCGGCaa                                                                                                       <  2:325014/66‑1 (MQ=255)                                                                                            gggCGAATGCCCCTGCCTGGTGTATCTAAGGGTTACAACTCATTCAGTATGTACTGGAACCGGCaa                                                                                                       >  1:325014/1‑66 (MQ=255)                                                                                            gggCGAATGCCCCTGCCTGGTGTATCTAAGGGTTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAg                              >  2:780477/1‑139 (MQ=255)                                                                                                                     cTAAGGGTTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGt                                       >  2:140805/1‑105 (MQ=255)                                                                                                                     cTAAGGGTTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGt                                       <  1:140805/105‑1 (MQ=255)                                                                                                                     cTAAGGGTTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGAtt                    <  1:949272/124‑1 (MQ=255)                                                                                                                     cTAAGGGTTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGAtt                    <  1:642972/124‑1 (MQ=255)                                                                                                                     cTAAGGGTTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGAtt                    >  2:642972/1‑124 (MQ=255)                                                                                                                     cTAAGGGTTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGAtt                    >  2:949272/1‑124 (MQ=255)                                                                                                                        aGGGTTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTc  <  2:322207/139‑1 (MQ=255)                                                                                                                            |                                                                                                                                       CGTTGTTGGTGTTGATTGAAGTCGAAGAGTGGCTGCCGCCGGAGTTGCCCTGAGGAGTTGAGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTC  >  NC_000913/2449105‑2449362

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑