Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,525,930 C→T *309* (TAG→TAA yfeR ← transcriptional regulator of yefH

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,525,9300CT100.0% 146.5 / NA 46*309* (TAG→TAAyfeRtranscriptional regulator of yefH
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (21/25);  total (21/25)

CAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGTTCTTCCTGCGCACCA  >  NC_000913/2525811‑2526048
                                                                                                                       |                                                                                                                      
acggggattcagtatcgatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTtata                                                                                                                    <  2:432437‑M2/9‑1 (MQ=255)
acggggattcagtatcgatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTtata                                                                                                                    >  1:432437‑M2/116‑124 (MQ=255)
      attcagtatcgatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTtat                                                                                                                     <  2:793264‑M2/8‑1 (MQ=255)
      attcagtatcgatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTtat                                                                                                                     >  1:793264‑M2/110‑117 (MQ=255)
      attcagtatcgatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACg                                                                                                        <  1:788164‑M2/21‑1 (MQ=255)
      attcagtatcgatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACg                                                                                                        >  2:788164‑M2/110‑130 (MQ=255)
       ttcagtatcgatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgactactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTc                                                                                              <  1:403896‑M2/31‑1 (MQ=255)
              tcgatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGccc                                                                                        <  1:21047‑M2/37‑1 (MQ=255)
              tcgatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGccc                                                                                        >  2:21047‑M2/102‑138 (MQ=255)
                gatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATc                                                                                                    >  1:94208‑M2/100‑124 (MQ=255)
                gatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATc                                                                                                    <  2:94208‑M2/25‑1 (MQ=255)
                 atatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCt                                                                                                                 <  2:496447‑M2/12‑1 (MQ=255)
                 atatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCt                                                                                                                 >  1:496447‑M2/99‑110 (MQ=255)
                          tgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCa                                                                           <  1:939345‑M2/50‑1 (MQ=255)
                                actgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGccc                                                                     <  1:191549‑M2/56‑1 (MQ=255)
                                                     ggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACg                                                                                                        >  1:168990‑M2/63‑83 (MQ=255)
                                                     ggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACg                                                                                                        <  2:168990‑M2/21‑1 (MQ=255)
                                                     ggatgcgataatctactccatcaaatttaacttctaattgattactgccggggctcccggcaCACTTtata                                                                                                                    >  1:614717‑M2/63‑71 (MQ=255)
                                                               atctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAg                                      <  1:322979‑M2/87‑1 (MQ=255)
                                                                       catcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATc                                                                                                    <  1:390609‑M2/25‑1 (MQ=255)
                                                                       catcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATc                                                                                                    >  2:390609‑M2/45‑69 (MQ=255)
                                                                       catcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTgg                                                                  <  2:118275‑M2/59‑1 (MQ=255)
                                                                       catcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTgg                                                                  >  1:118275‑M2/45‑103 (MQ=255)
                                                                       catcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTg                              >  1:309379‑M2/45‑139 (MQ=255)
                                                                         tcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCa                                                                           <  2:471106‑M2/50‑1 (MQ=255)
                                                                         tcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCa                                                                           >  1:471106‑M2/43‑92 (MQ=255)
                                                                         tcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGt                                                     <  1:100806‑M2/72‑1 (MQ=255)
                                                                         tcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGt                                                     >  2:100806‑M2/43‑114 (MQ=255)
                                                                               ttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGa                      <  2:309379‑M2/103‑1 (MQ=255)
                                                                                taacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGaa                     <  1:486220‑M2/104‑1 (MQ=255)
                                                                                 aacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTAcc                                                         >  1:47621‑M2/35‑102 (MQ=255)
                                                                                 aacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTAcc                                                         <  2:47621‑M2/68‑1 (MQ=255)
                                                                                             attactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTccc                                                                                            <  1:187465‑M2/33‑1 (MQ=255)
                                                                                             attactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTccc                                                                                            >  2:187465‑M2/23‑55 (MQ=255)
                                                                                               tactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTAcc                                                         <  2:500366‑M2/68‑1 (MQ=255)
                                                                                               tactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTAcc                                                         >  1:500366‑M2/21‑88 (MQ=255)
                                                                                                   gccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGaa                     <  2:1001879‑M2/104‑1 (MQ=255)
                                                                                                   gccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGaa                     >  1:1001879‑M2/17‑120 (MQ=255)
                                                                                                       gggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCa                                                                           <  1:516654‑M2/50‑1 (MQ=255)
                                                                                                       gggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCa                                                                           >  2:516654‑M2/13‑62 (MQ=255)
                                                                                                       gggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAgcggc                                  <  1:508341‑M2/91‑1 (MQ=255)
                                                                                                       gggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAgcggc                                  >  2:508341‑M2/13‑103 (MQ=255)
                                                                                                            cccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGTTCTTCCTGCGCACCa  >  1:945579‑M2/8‑130 (MQ=255)
                                                                                                            cccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGTTCTTCCTGCGCACCa  <  2:945579‑M2/123‑1 (MQ=255)
                                                                                                              cggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCa                                                                                       >  1:143870‑M2/6‑43 (MQ=255)
                                                                                                              cggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCa                                                                                       <  2:143870‑M2/38‑1 (MQ=255)
                                                                                                                       |                                                                                                                      
CAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGTTCTTCCTGCGCACCA  >  NC_000913/2525811‑2526048

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: