Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	255,659	A→G	C20R (TGT→CGT)	pepD ←	aminoacyl‑histidine dipeptidase (peptidase D)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	255,659	0	A	G	100.0%	189.2 / NA	52	C20R (TGT→CGT)	pepD	aminoacyl‑histidine dipeptidase (peptidase D)
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (29/23);  total (29/23)

CGGTAGCAGGTTTACGAATCAGGATATTACCTACCTGATCGCGTTCGACATGGAAACCTTTCTCTTTTGCCCAACCAACAATGTATTCAGCGAGTTGCTCTTCATGATAGGACGGGTGAGGAATAGAACAGATTTTGGCAAAAATATCCCACAGCGGCTGTGGAGATAATTGAGACAGTTCAGACACGTTAAGTCTCCTTGTCGATCACCCGCAAAACAGTATTGCAGGTCACAGGGTTAGCAGAAAATGTTGTCAACACAAGA  >  NC_000913/255530‑255793                                                                                                                                  |                                                                                                                                       cGGTAGCAGGTTTACGAATCAGGATATTACCTACCTGATCGCGTTCGACATGGAAACCTTTCTCTTTTGCCCAACCAACAATGTATTCAGCGAGTTGCTCTTCATGATAGGACGGGTGAGGAATAGAACGGATTTTGGc                                                                                                                               <  1:477887/139‑1 (MQ=255)           tttACGAATCAGGATATTACCTACCTGATCGCGTTCGACATGGAAACCTTTCTCTTTTGCCCAACCAACAATGTATTCAGCGAGTTGCTCTTCATGATAGGACGGGTGAGGAATAGAACGGATTTTGGCaa                                                                                                                             <  2:366120/131‑1 (MQ=255)           tttACGAATCAGGATATTACCTACCTGATCGCGTTCGACATGGAAACCTTTCTCTTTTGCCCAACCAACAATGTATTCAGCGAGTTGCTCTTCATGATAGGACGGGTGAGGAATAGAACGGATTTTGGCaa                                                                                                                             >  1:366120/1‑131 (MQ=255)              aCGAATCAGGATATTACCTACCTGATCGCGTTCGACATGGAAACCTTTCTCTTTTGCCCAACCAACAATGTATTCAGCGAGTTGCTCTTCATGATAGGACGGGTGAGGAATAGAACGGATTTTGGCAAAAATATCCcac                                                                                                                  >  1:532711/1‑139 (MQ=255)                gAATCAGGATATTACCTACCTGATCGCGTTCGACATGGAAACCTTTCTCTTTTGCCCAACCAACAATGTATTCAGCGAGTTGCTCTTCATGATAGGACGGGTGAGGAATAGAACGGATTTTGGCAAAAATATCCCACAg                                                                                                                <  2:781235/139‑1 (MQ=255)                       gATATTACCTACCTGATCGCGTTCGACATGGAAACCTTTCTCTTTTGCCCAACCAACAATGTATTCAGCGAGTTGCTCTTCATGATAGGACGGGTGAGGAATAGAACGGATTTTGGCAAAAATATCCCACAGCGGCtgt                                                                                                         <  2:978308/139‑1 (MQ=255)                       gATATTACCTACCTGATCGCGTTCGACATGGAAACCTTTCTCTTTTGCCCAACCAACAATGTATTCAGCGAGTTGCTCTTCATGATAGGACGGGTGAGGAATAGAACGGATTTTGGCAAAAATATCCCACAGCGGCtgt                                                                                                         >  1:380913/1‑139 (MQ=255)                                 acctGATCGCGTTCGACATGGAAACCTTTCTCTTTTGCCCAACCAACAATGTATTCAGCGAGTTGCTCTTCATGATAGGACGGGTGAGGAATAGAACGGATTTTGGCAAAAata                                                                                                                        <  2:945563/114‑1 (MQ=255)                                 acctGATCGCGTTCGACATGGAAACCTTTCTCTTTTGCCCAACCAACAATGTATTCAGCGAGTTGCTCTTCATGATAGGACGGGTGAGGAATAGAACGGATTTTGGCAAAAata                                                                                                                        >  1:945563/1‑114 (MQ=255)                                   ctGATCGCGTTCGACATGGAAACCTTTCTCTTTTGCCCAACCAACAATGTATTCAGCGAGTTGCTCTTCATGATAGGACGGGTGAGGAATAGAACGGATTTTGGCAAAAATATCCCACAGCGGCTGTGGAGATAATTga                                                                                             >  2:873419/1‑139 (MQ=255)                                      aTCGCGTTCGACATGGAAACCTTTCTCTTTTGCCCAACCAACAATGTATTCAGCGAGTTGCTCTTCATGATAGGACGGGTGAGGAATAGAACGGATTTTGGCAAAAATATCCCACAg                                                                                                                >  2:307261/1‑117 (MQ=255)                                      aTCGCGTTCGACATGGAAACCTTTCTCTTTTGCCCAACCAACAATGTATTCAGCGAGTTGCTCTTCATGATAGGACGGGTGAGGAATAGAACGGATTTTGGCAAAAATATCCCACAg                                                                                                                <  1:307261/117‑1 (MQ=255)                                      aTCGCGTTCGACATGGAAACCTTTCTCTTTTGCCCAACCAACAATGTATTCAGCGAGTTGCTCTTCATGATAGGACGGGTGAGGAATAGAACGGATTTTGGCAAAAATATCCCACAGCgg                                                                                                             >  2:656475/1‑120 (MQ=255)                                      aTCGCGTTCGACATGGAAACCTTTCTCTTTTGCCCAACCAACAATGTATTCAGCGAGTTGCTCTTCATGATAGGACGGGTGAGGAATAGAACGGATTTTGGCAAAAATATCCCACAGCgg                                                                                                             <  1:656475/120‑1 (MQ=255)                                      aTCGCGTTCGACATGGAAACCTTTCTCTTTTGCCCAACCAACAATGTATTCAGCGAGTTGCTCTTCATGATAGGACGGGTGAGGAATAGAACGGATTTTGGCAAAAATATCCCACAGCGGCTGTGGAGATAATTGAGAc                                                                                          >  2:822450/1‑139 (MQ=255)                                      aTCGCGTTCGACATGGAAACCTTTCTCTTTTGCCCAACCAACAATGTATTCAGCGAGTTGCTCTTCATGATAGGACGGGTGAGGAATAGAACGGATTTTGGCAAAAATATCCCACAGCGGCTGTGGAGATAATTGAGAc                                                                                          >  2:835006/1‑139 (MQ=255)                                             tCGACATGGAAACCTTTCTCTTTTGCCCAACCAACAATGTATTCAGCGAGTTGCTCTTCATGATAGGACGGGTGAGGAATAGAACGGATTTTGGCAAAAATATCTCACAGCGGCTGTGGAGATAATTGAGACAGTTCAg                                                                                   >  2:529996/1‑139 (MQ=255)                                                        aCCTTTCTCTTTTGCCCAACCAACAATGTATTCAGCGAGTTGCTCTTCATGATAGGACGGGTGAGGAATAGAACGGATTTTGGCAAAAATATCCCACAGCGGCTGTGGAGATAATTGAGACAGTTCAGACACGTTAAGt                                                                        <  1:974619/139‑1 (MQ=255)                                                          cTTTCTCTTTTGCCCAACCAACAATGTATTCAGCGAGTTGCTCTTCATGATAGGACGGGTGAGGAATAGAACGGATTTTGGCAAAAATATCCCACAGCGGCTGTGGAGATAATTGAGACAGTTCAGACACGTTAAg                                                                         <  2:439427/136‑1 (MQ=255)                                                          cTTTCTCTTTTGCCCAACCAACAATGTATTCAGCGAGTTGCTCTTCATGATAGGACGGGTGAGGAATAGAACGGATTTTGGCAAAAATATCCCACAGCGGCTGTGGAGATAATTGAGACAGTTCAGACACGTTAAg                                                                         >  1:439427/1‑136 (MQ=255)                                                                     gCCCAACCAACAATGTATTCAGCGAGTTGCTCTTCATGATAGGACGGGTGAGGAATAGAACGGATTTTGGCAAAAATATCCCACAGCGGCTGTGGAGATAATTGAGACAGTTCAGACACGTTAAGTCTCCTTGTCGATc                                                           <  1:822450/139‑1 (MQ=255)                                                                      cccAACCAACAATGTATTCAGCGAGTTGCTCTTCATGATAGGACGGGTGAGGAATAGAACGGATTTTGGCAAAAATATCCCACAGCGGCTGTGGAGATAATTGAGACAGTTCAGACACGTTAAGTCTCCTTGTCGATCa                                                          >  1:698824/1‑139 (MQ=255)                                                                        caaccaacAATGTATTCAGCGAGTTGCTCTTCATGATAGGACGGGTGAGGAATAGAACGGATTTTGGCAAAAATATCCCACAGCGGCTGTGGAGATAATTGAGACAGTTCAGACACGTTAAGTCTCCTTGTCGATCAcc                                                        <  1:835006/139‑1 (MQ=255)                                                                                 aTGTATTCAGCGAGTTGCTCTTCATGATAGGACGGGTGAGGAATAGAACGGATTTTGGCAAAAATATCCCACAGCGGCTGTGGAGATAATTGAGACAGTTCAGACACGTTAAGTCTCCTTGTCGATCACCCGCAAAACa                                               >  2:603596/1‑139 (MQ=255)                                                                                       tCAGCGAGTTGCTCTTCATGATAGGACGGGTGAGGAATAGAACGGATTTTGGCAAAAATATCCCACAGCGGCTGTGGAGATAATTGAGACAGTTCAGACACGTTAAGTCTCCTTGTCGATCACCCGCAAAACAGTATTg                                         >  2:463502/1‑139 (MQ=255)                                                                                         aGCGAGTTGCTCTTCATGATAGGACGGGTGAGGAATAGAACGGATTTTGGCAAAAATATCCCACAGCGGCt                                                                                                           >  2:199715/1‑71 (MQ=255)                                                                                         aGCGAGTTGCTCTTCATGATAGGACGGGTGAGGAATAGAACGGATTTTGGCAAAAATATCCCACAGCGGCt                                                                                                           <  1:199715/71‑1 (MQ=255)                                                                                         aGCGAGTTGCTCTTCATGATAGGACGGGTGAGGAATAGAACGGATTTTGGCAAAAATATCCCACAGCGGCTGTGGAGATAATTGAGACAGTTCAGACACGTTAAGTCTCCTTGTCGATc                                                           <  2:637082/119‑1 (MQ=255)                                                                                         aGCGAGTTGCTCTTCATGATAGGACGGGTGAGGAATAGAACGGATTTTGGCAAAAATATCCCACAGCGGCTGTGGAGATAATTGAGACAGTTCAGACACGTTAAGTCTCCTTGTCGATc                                                           >  1:637082/1‑119 (MQ=255)                                                                                            gAGTTGCTCTTCATGATAGGACGGGTGAGGAATAGAACGGATTTTGGCAAAAATATCCCACAGCGGCTGTGGAGATAATTGAGACAGTTCAGACACGTTAAGTCTCCTTGTCGATCACCCGCAAAACAGTATTGCAGGt                                    >  2:915839/1‑139 (MQ=255)                                                                                                tGCTCTTCATGATAGGACGGGTGAGGAATAGAACGGATTTTGGCAAAAATATCCCACAGCGGCTGTGGAGATAATTGAGACAGTTCAGACACGTTAAGTCTCCTTGTCGATCACCCGCAAAACAGTATTGc                                        >  2:986412/1‑131 (MQ=255)                                                                                                tGCTCTTCATGATAGGACGGGTGAGGAATAGAACGGATTTTGGCAAAAATATCCCACAGCGGCTGTGGAGATAATTGAGACAGTTCAGACACGTTAAGTCTCCTTGTCGATCACCCGCAAAACAGTATTGc                                        <  1:986412/131‑1 (MQ=255)                                                                                                tGCTCTTCATGATAGGACGGGTGAGGAATAGAACGGATTTTGGCAAAAATATCCCACAGCGGCTGTGGAGATAATTGAGACAGTTCAGACACGTTAAGTCTCCTTGTCGATCACCCGCAAAACAGTATTGCAGGTcaca                                >  1:44012/1‑139 (MQ=255)                                                                                                         tGATAGGACGGGTGAGGAATAGAACGGATTTTGGCAAAAATATCCCACAGCGGCTGTGGAGATAATTGAGACAGTTCAGACACGTTAAGTCTCCTTGTCGATCACCCGCAAAACAGTATTGCGGGTCACAGGGTTAGCa                       >  1:836146/1‑139 (MQ=255)                                                                                                         tGATAGGACGGGTGAGGAATAGAACGGATTTTGGCAAAAATATCCCACAGCGGCTGTGGAGATAATTGAGACAGTTCAGACACGTTAAGTCTCCTTGTCGATCACCCGCAAAACAGTATTGCAGGTCACAGGGTTAGCa                       >  1:195550/1‑139 (MQ=255)                                                                                                            tAGGACGGGTGAGGAATAGAACGGATTTTGGCAAAAATATCCCACAGCGGCTGTGGAGATAATTGAGACAGTTCAGACACGTTAAGTCTCCTTGTCGATCACCCGCAAAACAGTATTGCAGGTCACAGGGTTAGCAGaa                    >  1:16490/1‑139 (MQ=255)                                                                                                               gACGGGTGAGGAATAGAACGGATTTTGGCAAAAATATCCCACAg                                                                                                                >  1:962362/1‑44 (MQ=255)                                                                                                               gACGGGTGAGGAATAGAACGGATTTTGGCAAAAATATCCCACAg                                                                                                                <  2:962362/44‑1 (MQ=255)                                                                                                               gACGGGTGAGGAATAGAACGGATTTTGGCAAAAATATCCCACAGCGGCTGTGgag                                                                                                     >  2:324174/1‑55 (MQ=255)                                                                                                               gACGGGTGAGGAATAGAACGGATTTTGGCAAAAATATCCCACAGCGGCTGTGgag                                                                                                     <  1:324174/55‑1 (MQ=255)                                                                                                               gACGGGTGAGGAATAGAACGGATTTTGGCAAAAATATCCCACAGCGGCTGTGGAGATAATTGAGACAGTTCAGACACGTTAAGTCTCCTTGTCGATCACCCGCAAAACAGTATTGCAGGTCACAGGGTTAGCAGAAAAt                 <  2:16490/139‑1 (MQ=255)                                                                                                                 cGGGTGAGGAATAGAACGGATTTTGGCAAAAATATCCCACAGCGGCTGTGGAGATAATTGAGACAGTTCAGACACGTTAAGTCTCCTTGTCGATCACCCGCAAAACAGTATTg                                         <  1:740472/113‑1 (MQ=255)                                                                                                                 cGGGTGAGGAATAGAACGGATTTTGGCAAAAATATCCCACAGCGGCTGTGGAGATAATTGAGACAGTTCAGACACGTTAAGTCTCCTTGTCGATCACCCGCAAAACAGTATTg                                         >  2:740472/1‑113 (MQ=255)                                                                                                                    gTGAGGAATAGAACGGATTTTGGCAAAAATATCCCACAGCgg                                                                                                             <  2:297153/42‑1 (MQ=255)                                                                                                                    gTGAGGAATAGAACGGATTTTGGCAAAAATATCCCACAGCgg                                                                                                             >  1:297153/1‑42 (MQ=255)                                                                                                                      gAGGAATAGAACGGATTTTGGCAAAAATATCCCACAGCGGCAGTGGAGATAATTGAGACAGTTCAGACACGTTAAGTCTCCTTGTCGATCACCCGCAAAACAGTATTGCAGGTCACAGGGTTAGCAGAAAATGTTGTCa          >  1:623928/1‑139 (MQ=255)                                                                                                                       aGGAATAGAACGGATTTTGGCAAAAATATCCCACAGCGGCTGTGGAGATAATTGAGACAGTTCa                                                                                    >  1:138994/1‑64 (MQ=255)                                                                                                                       aGGAATAGAACGGATTTTGGCAAAAATATCCCACAGCGGCTGTGGAGATAATTGAGACAGTTCa                                                                                    <  2:138994/64‑1 (MQ=255)                                                                                                                         gAATAGAACGGATTTTGGCAAAAATATCCCACAGCGGCTGTGGAGATAATTGAGACAGTTCAGACACGTTAAGTCTCCTTGTCGATCACCCGCAAAACAGTATTGCAGGTCACAGGGTTAGCAGAAAATGTTGTCAaca       <  1:603596/139‑1 (MQ=255)                                                                                                                              gAACGGATTTTGGCAAAAATATCCCACAGCGGCTGTGGAGATAATTGAGACAGTTCAGACACGTTAAGTCTCCTTGTCGATCAcc                                                        <  1:685725/85‑1 (MQ=255)                                                                                                                              gAACGGATTTTGGCAAAAATATCCCACAGCGGCTGTGGAGATAATTGAGACAGTTCAGACACGTTAAGTCTCCTTGTCGATCACCCGCAAAACAGTATTGCAGGTCACAGGGTTAGCAGAAAATGTTGTCAACACAAGa  >  2:165841/1‑139 (MQ=255)                                                                                                                               aaCGGATTTTGGCAAAAATATCCCACAGCGGCTGTGGAGATAATTGAGACAGTTCAGACACGTTAAGTCTCCTTGTCGATCACCCGCAAAACAGTATTGCAGGTCACAGGGTTAGCAGAAAAtgttgt            <  1:761038/128‑1 (MQ=255)                                                                                                                                  |                                                                                                                                       CGGTAGCAGGTTTACGAATCAGGATATTACCTACCTGATCGCGTTCGACATGGAAACCTTTCTCTTTTGCCCAACCAACAATGTATTCAGCGAGTTGCTCTTCATGATAGGACGGGTGAGGAATAGAACAGATTTTGGCAAAAATATCCCACAGCGGCTGTGGAGATAATTGAGACAGTTCAGACACGTTAAGTCTCCTTGTCGATCACCCGCAAAACAGTATTGCAGGTCACAGGGTTAGCAGAAAATGTTGTCAACACAAGA  >  NC_000913/255530‑255793

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑