Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,824,491	C→T	*362* (TAG→TAA)	mltB ←	membrane‑bound lytic murein transglycosylase B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,824,491	0	C	T	100.0%	111.8 / NA	36	*362* (TAG→TAA)	mltB	membrane‑bound lytic murein transglycosylase B
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (15/21);  total (15/21)

TCAGTCAAAAATTTATGCCGGACAAGATAGCACTTTCGTCAGGAAGATGGTGTTGGCGTTGCAATTTTACGAGGAGGATTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACG‑CCCCCCTCTTGTAAATAGCTACTGTACTCGCGCCAGCGCCACGGCTTGTCCTAACTGCCAGACCGCCATTGCGTAATGGGTGCTGTGGTTGTAACGGGTGATGGTGTAGAAGTTCGGCAGACCGTACCAGTACTGGTA  >  NC_000913/2824358‑2824610                                                                                                                                       |                                                                                                                        tcagtcaAAAATTTATGCCGGACAAGATAGCACTTTCGTCAGGAAGATGGTGTTGGCGTTGCAATTTTACGAGGAGGATTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACGCCCCCCCTCTTGTAAATAGTTACt                                                                                                                     >  1:168565/1‑139 (MQ=255)                           aTAGCACTTTCGTCAGGAAGATGGTGTTGGCGTTGCAATTTTACGAGGAGGATTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACGCCCCCCCTCTTGTAAATAGTTACT‑TACTCGCGCCAGCGCCACGGCTTGTc                                                                                          <  2:486789/139‑1 (MQ=255)                           aTAGCACTTTCGTCAGGAAGATGGTGTTGGCGTTGCAATTTTACGAGGAGGATTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACGCCCCCCCTCTTGTAAATAGTTACT‑TACTCGCGCCAGCGCCACGGCTTGTc                                                                                          <  2:168565/139‑1 (MQ=255)                                  tttCGTCAGGAAGATGGTGTTGGCGTTGCAATTTTACGAGGAGGATTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACGCCCCCCCTCTTGTAAATAGTTACT‑TACTCGCGCCAGCGCCACgg                                                                                                <  2:934275/126‑1 (MQ=255)                                  tttCGTCAGGAAGATGGTGTTGGCGTTGCAATTTTACGAGGAGGATTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACGCCCCCCCTCTTGTAAATAGTTACT‑TACTCGCGCCAGCGCCACgg                                                                                                >  1:934275/1‑126 (MQ=255)                                    tcctCAGGAAGCTGGTGTTGGCGTTGCAATTTTACGAGGAGGATTCAGAAAAGAGCTGATTAGCCAGAGGGAAGCTCACGCCCCCCCTCTTGTAAATAGTTACT‑TACTCGCGCCAGCGCCACGGCTTGTCCTAACTGcc                                                                                 >  1:585248/4‑139 (MQ=255)                                    tCGTCAGGAAGATGGTGTTGGCGTTGCAATTTTACGAGGAGGATTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACGCCCCCCCTCTTGTAAATAGTTACT‑TACTCGCGCCAGCGCCACGGCTTGTCCTAACTGcc                                                                                 >  2:965452/1‑139 (MQ=255)                                                tGGTGTTGGCGTTGCAATTTTACGAGGAGGATTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACGCCCCCCCTCTTGTAAATAGTTACT‑TACTCGCGCCAGCGCCACGGCTTGTCCTAACTGCCAGACCGCCATTg                                                                     <  1:965452/139‑1 (MQ=255)                                                      tGGCGTTGCAATTTTACGAGGAGGATTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACGCCCCCCCTCTTGTAAATAGTTACT‑TACTCGCGCCAGCGCCACGGCTTGTCCt                                                                                        >  1:135609/1‑114 (MQ=255)                                                      tGGCGTTGCAATTTTACGAGGAGGATTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACGCCCCCCCTCTTGTAAATAGTTACT‑TACTCGCGCCAGCGCCACGGCTTGTCCt                                                                                        <  2:135609/114‑1 (MQ=255)                                                         cGTTGCAATTTTACGAGGAGGATTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACGCCCCCCCTCTTGTAAATAGTTACT‑TACTCGCGCCAGCGCCACGGCTTGTCCTAACTGCCAGACCGCCATTGCGTAATggg                                                            >  1:254690/1‑139 (MQ=255)                                                            tGCAATTTTACGAGGAGGATTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACGCCCCCCCTCTTGTAAATAGTTACT‑TACTCGCGCCAGCGCCACGGCTTGTCCTAACTGCCAGACCGCCATTGCGTAATGGGTGc                                                         <  2:585248/139‑1 (MQ=255)                                                             gCAATTTTACGAGGAGGATTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACGCCCCCCCTCTTGTAAATAGTTACT‑TACTCGCGCCAGCGCCACGGCTTGTCCTAACTGCCAGACCGCCATTGCGTAATGGGTGCt                                                        <  1:679098/139‑1 (MQ=255)                                                              cAATTTTACGAGGAGGATTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACGCCCCCCCTCTTGTAAATAGTTACT‑TACTCGCGCCAGCGCCACGGCTTGTCCTAACTGCCAGACCGCCATTGCGTAATGGGTGCtg                                                       <  1:259102/139‑1 (MQ=255)                                                                   ttACGAGGAGGATTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACGCCCCCCCTCTTGTAAATAGTTACT‑TACTCGCGCCAGCGCCACGGCTTGTCCTAACTGCCAGACCGCCATTGCGTAATGGGTGCTGTGGtt                                                  <  2:86486/139‑1 (MQ=255)                                                                             gaTTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACGCCCCCCCTCTTGTAAATAGTTACT‑TACTCGCGCCAGCGCCACGGCTTGTCCTAACTGCCAGACCGCCATTGCGTAATGGGTGCt                                                        <  1:701035/123‑1 (MQ=255)                                                                             gaTTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACGCCCCCCCTCTTGTAAATAGTTACT‑TACTCGCGCCAGCGCCACGGCTTGTCCTAACTGCCAGACCGCCATTGCGTAATGGGTGCt                                                        >  2:701035/1‑123 (MQ=255)                                                                               ttCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACGCCCCCCCTCTTGTAAATAGTTACT‑TACTCGCGCCAGCGCCACGGCTTGTCCTAACTGCCAGACCGCCATTGCGTAATGGGTGCTGTGGTTGTAACGGGTGAt                                      <  2:765829/139‑1 (MQ=255)                                                                                       aaaGCTGATTAGCCAGAGGGAAGCTCACGCCCCCCCTCTTGTAAATAGTTACT‑TACTCGCGCCAGCGCCACGGCTTGTCCTa                                                                                       <  2:948840/82‑1 (MQ=255)                                                                                       aaaGCTGATTAGCCAGAGGGAAGCTCACGCCCCCCCTCTTGTAAATAGTTACT‑TACTCGCGCCAGCGCCACGGCTTGTCCTa                                                                                       >  1:948840/1‑82 (MQ=255)                                                                                        aaGCTGATTAGCCAGAGGGAAGCTCACGCCCCCCCTCTTGTAAATAGTTACT‑TACTCGCGCCAGCGCCACGGCTTGTCCTAACTGCCAGACCGCCa                                                                        <  1:459067/96‑1 (MQ=255)                                                                                        aaGCTGATTAGCCAGAGGGAAGCTCACGCCCCCCCTCTTGTAAATAGTTACT‑TACTCGCGCCAGCGCCACGGCTTGTCCTAACTGCCAGACCGCCa                                                                        >  2:459067/1‑96 (MQ=255)                                                                                            tGATTAGCCAGAGGGAAGCTCACGCCCCCCCTCTTGTAAATAGTTACT‑TACTCgcgccagcgcc                                                                                                    >  2:50711/1‑64 (MQ=255)                                                                                            tGATTAGCCAGAGGGAAGCTCACGCCCCCCCTCTTGTAAATAGTTACT‑TACTCgcgccagcgcc                                                                                                    <  1:50711/64‑1 (MQ=255)                                                                                              aTTAGCCAGAGGGAAGCTCACGCCCCCCCTCTTGTAAATAGTTACT‑TACTCGCGCCAGCGCCACGGCTTGTCCTAACTGCCAGACCGCCATTGCGTAATGGGTGCTGTGGTTGTAACGGGTGATGGTGTAGAAGTTCgg                       <  2:552668/139‑1 (MQ=255)                                                                                                         ggAAGCTCACGCCCCCCCTCTTGTAAATAGTTACT‑TACTCGCGCCAGCGCCACGGCTTGTCCTAACTGCCAGGCCGCCATTGCGTAATGGGTGCTGTGGTTGTAACGGGTGATGGTGTAGAAGTTCGGCAGACCGTAcc            <  1:263318/139‑1 (MQ=255)                                                                                                         ggAAGCTCACGCCCCCCCTCTTGTAAATAGTTACT‑TACTCGCGCCAGCGCCACGGCTTGTCCTAACTGCCAGACCGCCATTGCGTAATGGGTGCTGTGGTTGTAACGGGTGATGGTGTAGAAGTTCGGCAGACCGTAcc            <  2:254690/139‑1 (MQ=255)                                                                                                            aGCTCACGCCCCCCCTCTTGTAAATAGTTACT‑TACTCGCGCCAGCGCCACGGCTTGTCCTAACTGCCAGACCGCCATTGCGTAATGGGTGCt                                                        <  2:475375/92‑1 (MQ=255)                                                                                                            aGCTCACGCCCCCCCTCTTGTAAATAGTTACT‑TACTCGCGCCAGCGCCACGGCTTGTCCTAACTGCCAGACCGCCATTGCGTAATGGGTGCt                                                        >  1:475375/1‑92 (MQ=255)                                                                                                             gCTCACGCCCCCCCTCTTGTAAATAGTTACT‑TACTCGCGCCAGCGCCACGGCTTGTCCTAACTGCCAGACCGCCATTGCGTAATGGGTGCTGTGGTTGTAACGGGTGATGGTGTAGAAGTTCGGCAGACCGTa              >  2:404530/1‑133 (MQ=255)                                                                                                             gCTCACGCCCCCCCTCTTGTAAATAGTTACT‑TACTCGCGCCAGCGCCACGGCTTGTCCTAACTGCCAGACCGCCATTGCGTAATGGGTGCTGTGGTTGTAACGGGTGATGGTGTAGAAGTTCGGCAGACCGTa              <  1:404530/133‑1 (MQ=255)                                                                                                                   gcccccccTCTTGTAAATAGTTACT‑TACTCGCGCCAGCGCCACGGCTTGTCCTAACTGCCAGACCGCCATTGCGTAATGGGTGCTGTGGTTGTAACGGGTGATGGTGTAGAAGTTCGGCAGACCGTACCAGTACTGGTa  >  1:828691/3‑139 (MQ=255)                                                                                                                               gTAAATAGTTACT‑TACTCGCGCCAGCGCCACGGCTTGTCCTAACTGCCAGACCGCCATTGCGTAATGGGTGCTGTGGTTGTAACGGGt                                         <  2:375767/88‑1 (MQ=255)                                                                                                                               gTAAATAGTTACT‑TACTCGCGCCAGCGCCACGGCTTGTCCTAACTGCCAGACCGCCATTGCGTAATGGGTGCTGTGGTTGTAACGGGt                                         >  1:375767/1‑88 (MQ=255)                                                                                                                               gTAAATAGTTACT‑TACTCGCGCCAGCGCCACGGCTTGTCCTAACTGCCAGACCGCCATTGCGTAATGGGTGCTGTGGTTGTAACGGGTGATGGTGTAGAAGTTCGGCAGAc                  <  2:246827/111‑1 (MQ=255)                                                                                                                               gTAAATAGTTACT‑TACTCGCGCCAGCGCCACGGCTTGTCCTAACTGCCAGACCGCCATTGCGTAATGGGTGCTGTGGTTGTAACGGGTGATGGTGTAGAAGTTCGGCAGAc                  >  1:246827/1‑111 (MQ=255)                                                                                                                                       |                                                                                                                        TCAGTCAAAAATTTATGCCGGACAAGATAGCACTTTCGTCAGGAAGATGGTGTTGGCGTTGCAATTTTACGAGGAGGATTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACG‑CCCCCCTCTTGTAAATAGCTACTGTACTCGCGCCAGCGCCACGGCTTGTCCTAACTGCCAGACCGCCATTGCGTAATGGGTGCTGTGGTTGTAACGGGTGATGGTGTAGAAGTTCGGCAGACCGTACCAGTACTGGTA  >  NC_000913/2824358‑2824610

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑