Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,831,146 G→A L382F (CTC→TTC)  norR ← anaerobic nitric oxide reductase DNA‑binding transcriptional activator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,831,1460GA100.0% 69.9 / NA 23L382F (CTC→TTC) norRanaerobic nitric oxide reductase DNA‑binding transcriptional activator
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (15/8);  total (15/8)

TCCAGTTCGCGCACGTTTCCCGGAAAACTGTAGTGTTGCAGTAAATTTCGCGCTCCGGCACTTAATACCACGCGGGAGAGCCCCTGCCGCAAACGACACTGCTCGCAGAAATACCCCGCCAGCAGAATGACATCATCGCCCCGCTCACGCAGCGGCGGCACCGAAAGTGGAAACACGCTCAGGCGATGAAACAAATCGGCGCGGAATCGCC  >  NC_000913/2831067‑2831277
                                                                               |                                                                                                                                   
tCCAGTTCGCGCACGTTTCCCGGAAAACTGTAGTGTTGCAGTAAATTTCGCGCTCCGGCACTTAATACCACGCGGGAGAACCCCTGCCGCAAACGACACTGCTCGCAGAAATACCCCGCCAGCAGAATGACATCATCGc                                                                          >  1:661393/1‑139 (MQ=255)
tCCAGTTCGCGCACGTTTCCCGGAAAACTGTAGTGTTGCAGTAAATTTCGCGCTCCGGCACTTAATACCACGCGGGAGAACCCCTGCCGCAAACGACACTGCTCGCAGAAATACCCCGCCAGCAGAATGACATCATCGc                                                                          >  2:1009339/1‑139 (MQ=255)
         cgcACGTTTCCCGGAAAACTGTAGTGTTGCAGTAAATTTCGCGCTCCGGCACTTAATACCACGCGGGAGAACCCCTGCCGCAAACGACACTGCTCGCAGAAATACCCCGCCAGCAGAATGACATCATCGCCCCGCTCAc                                                                 >  2:259213/1‑139 (MQ=255)
         cgcACGTTTCCCGGAAAACTGTAGTGTTGCAGTAAATTTCGCGCTCCGGCACTTAATACCACGCGGGAGAACCCCTGCCGCAAACGACACTGCTCGCAGAAATACCCCGCCAGCAGAATGACATCATCGCCCCGCTCAc                                                                 >  1:938762/1‑139 (MQ=255)
                                   ttGCAGTAAATTTCGCGCTCCGGCACTTAATACCACGCGGGAGAACCCCTGc                                                                                                                              >  1:447169/1‑52 (MQ=255)
                                   ttGCAGTAAATTTCGCGCTCCGGCACTTAATACCACGCGGGAGAACCCCTGc                                                                                                                              <  2:447169/52‑1 (MQ=255)
                                      cAGTAAATTTCGCGCTCCGGCACTTAATACCACGCGGGAGAACCCCTGCCGCAAACGACACTGCTCGCAGAAATACCCCGCCAGCAGAATGACATCATCGc                                                                          >  2:149127/1‑101 (MQ=255)
                                      cAGTAAATTTCGCGCTCCGGCACTTAATACCACGCGGGAGAACCCCTGCCGCAAACGACACTGCTCGCAGAAATACCCCGCCAGCAGAATGACATCATCGc                                                                          <  1:149127/101‑1 (MQ=255)
                                               tCGCGCTCCGGCACTTAATACCACGCGGGAGAACCCCTGCCGCAAACGACACTGCTCGCAGAAATACCCCGCCAGCAGAATGACATCATCGCCCCGCTCACGCAGCGGCGGCACCGAAAGTGGAAACACGCTCAGGCGa                           <  1:259213/139‑1 (MQ=255)
                                                cgcgCTCCGGCACTTAATACCACGCGGGAGAACCCCTGcc                                                                                                                             <  1:511615/40‑1 (MQ=255)
                                                cgcgCTCCGGCACTTAATACCACGCGGGAGAACCCCTGcc                                                                                                                             >  2:511615/1‑40 (MQ=255)
                                                                      cgcgGGAGAACCCCTGCCGCAAACGACACTGCTCGCAGAAATACCCCGCCAGCAGAATGACATCATCGCCCCGCTCACGCAGCGGCGGCACCGAAAGTGGAAACACGCTCAGGCGATGAAACAAATCGGCGCGGAATCg    >  2:657634/1‑139 (MQ=255)
                                                                       gcgGGAGAACCCCTGCCGCAAACGACACTGCTCGCAGAAATACCCCGCCAGCAGAATGACATCATCGCCCCGCTCACGCAGCGGCGGCACCGAAAg                                              >  2:70629/1‑96 (MQ=255)
                                                                       gcgGGAGAACCCCTGCCGCAAACGACACTGCTCGCAGAAATACCCCGCCAGCAGAATGACATCATCGCCCCGCTCACGCAGCGGCGGCACCGAAAg                                              <  1:70629/96‑1 (MQ=255)
                                                                       gcgGGAGAACCCCTGCCGCAAACGACACTGCTCGCAGAAATACCCCGCCAGCAGAATGACATCATCGCCCCGCTCACGCAGCGGCGGCACCGAAAGt                                             <  1:59800/97‑1 (MQ=255)
                                                                       gcgGGAGAACCCCTGCCGCAAACGACACTGCTCGCAGAAATACCCCGCCAGCAGAATGACATCATCGCCCCGCTCACGCAGCGGCGGCACCGAAAGt                                             >  2:59800/1‑97 (MQ=255)
                                                                       gcgGGAGAACCCCTGCCGCAAACGACACTGCTCGCAGAAATACCCCGCCAGCAGAATGACATCATCGCCCCGCTCACGCAGCGGCGGCACCGAAAGTGGAAac                                       >  2:200762/1‑103 (MQ=255)
                                                                       gcgGGAGAACCCCTGCCGCAAACGACACTGCTCGCAGAAATACCCCGCCAGCAGAATGACATCATCGCCCCGCTCACGCAGCGGCGGCACCGAAAGTGGAAac                                       <  1:200762/103‑1 (MQ=255)
                                                                       gcgGGAGAACCCCTGCCGCAAACGACACTGCTCGCAGAAATACCCCGCCAGCAGAATGACATCATCGCCCCGCTCACGCAGCGGCGGCACCGAAAGTGGAAACACGCTCAGGCGATGAAACAAATCGGCGCGGAATCGc   >  1:529593/1‑139 (MQ=255)
                                                                       gcgGGAGAACCCCTGCCGCAAACGACACTGCTCGCAGAAATACCCCGCCAGCAGAATGACATCATCGCCCCGCTCACGCAGCGGCGGCACCGAAAGTGGAAACACGCTCAGGCGATGAAACAAATCGGCGCGGAATCGc   >  2:69284/1‑139 (MQ=255)
                                                                        cgGGAGAACCCCTGCCGCAAACGACACTGCTCGCAGAAATACCCCGCCAGCAGAATGACATCATCGCCCCGCTCACGCAGCGGCGGCACCGAAAGTGGAAACACGCTCAGGCGATGAAACAAATCGGCGCGGAATCGcc  >  1:862984/1‑139 (MQ=255)
                                                                           gagaACCCCTGCCGCAAACGACACTGCTCGCAGAAATACCCCGCCAGCAGAATGACATCATCGCCCCGCTCACGCAGCGGCGGCACCGAAAGTGGAAACACGCTCAGGCGATGAAACAAATCGGCGCGGAATCGcc  <  2:96797/136‑1 (MQ=255)
                                                                           gagaACCCCTGCCGCAAACGACACTGCTCGCAGAAATACCCCGCCAGCAGAATGACATCATCGCCCCGCTCACGCAGCGGCGGCACCGAAAGTGGAAACACGCTCAGGCGATGAAACAAATCGGCGCGGAATCGcc  >  1:96797/1‑136 (MQ=255)
                                                                               |                                                                                                                                   
TCCAGTTCGCGCACGTTTCCCGGAAAACTGTAGTGTTGCAGTAAATTTCGCGCTCCGGCACTTAATACCACGCGGGAGAGCCCCTGCCGCAAACGACACTGCTCGCAGAAATACCCCGCCAGCAGAATGACATCATCGCCCCGCTCACGCAGCGGCGGCACCGAAAGTGGAAACACGCTCAGGCGATGAAACAAATCGGCGCGGAATCGCC  >  NC_000913/2831067‑2831277

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: