| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,846,222 | T→C | T84A (ACT→GCT) | hycE ← | hydrogenase 3, large subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,846,222 | 0 | T | C | 100.0% | 103.3 / NA | 30 | T84A (ACT→GCT) | hycE | hydrogenase 3, large subunit |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (13/17); total (13/17) | |||||||||||
GAATCAAACCGTACATATCGCGCACTTCACGCTCGCCCCACACCGCCGCCGGAACGCGCGGCGTCACGGACGGATATTCCGGTTTGTTGGCGTCAACTTCGACGCGAACCGTAATCCAACACTTAGTGCCCTTCTCCATCGACAGCACGTAGTAAACGGCGTAATGACCATTCAGTTTGCGTTCGTCGTTACCAAACAGCACCGACAGCCAGCCACCCTGTTTGTAGTAAAGAAACTCCACCACTTCCGGCAGGTAGTTCAC > NC_000913/2846096‑2846357 | gAATCAAACCGTACATATCGCGCACTTCACGCTCGCCCCACACCGCCGCCGGAACGCGCGGCGTCACGGACGGATATTCCGGTTTGTTGGCGTCAACTTCGACGCGAACCGTAATCCAACACTTAGCGCCCTTCTCCAt < 1:893716/139‑1 (MQ=255) aCCGTACATATCGCGCACTTCACGCTCGCCCCACACCGCCGCCGGAACGCGCGGCGTCACGGACGGATATTCCGGTTTGTTGGCGTCAACTTCGACGCGAACCGTAATCCAACACTTAGCGCCCTTCTCCATCGACAGc < 1:477038/139‑1 (MQ=255) tCGCGCACTTCACGCTCGCCCCACACCGCCGCCGGAACGCGCGGCGTCACGGACGGATATTCCGGTTTGTTGGCGTCAACTTCGACGCGAACCGTAATCCAACACTTAGCGCCCTTCTCCATCGACAGCACGTAGTaaa < 2:343363/139‑1 (MQ=255) gcgcACTTCACGCTCGCCCCACACCGCCGCCGGAACGCGCGGCGTCACGGACGGATATTCCGGTTTGTTGGCGTCAACTTCGACGCGAACCGTAATCCAACACTTAGCGCCCTTCTCCATCGACAGCACGTAGTAAACg < 2:568370/139‑1 (MQ=255) gcACTTCACGCTCGCCCCACACCGCCGCCGGAACGCGCGGCGTCACGGACGGATATTCCGGTTTGTTGGCGTCAACTTCGGCGCGAACCGTAATCCAACACTTAGCGCCCTTCTCCATCGACAGCACGTAGTAAACGGc > 1:949044/1‑139 (MQ=255) gcACTTCACGCTCGCCCCACACCGCCGCCGGAACGCGCGGCGTCACGGACGGATATTCCGGTTTGTTGGCGTCAACTTCGACGCGAACCGTAATCCAACACTTAGCGCCCTTCTCCATCGACAGCACGTAGTaa < 2:881876/134‑1 (MQ=255) gcACTTCACGCTCGCCCCACACCGCCGCCGGAACGCGCGGCGTCACGGACGGATATTCCGGTTTGTTGGCGTCAACTTCGACGCGAACCGTAATCCAACACTTAGCGCCCTTCTCCATCGACAGCACGTAGTaa > 1:881876/1‑134 (MQ=255) gcACTTCACGCTCGCCCCACACCGCCGCCGGAACGCGCGGCGTCACGGACGGATATTCCGGTTTGTTGGCGTCAACTTCGACGCGAACCGTAATCCAACACCTAGCGCCCTTCTCCATCGACAGCACGTAGTAAACGGc > 1:645078/1‑139 (MQ=255) ccccACACCGCCGCCGGAACGCGCGGCGTCACGGACGGATATTCCGGTTTGTTGGCGTCAACTTCGACGCGAACCGTAATCCAACACTTAGCGCCCTTCTCCATCGACAGCACGTAGTAAACGGCGTAATGACCATTCa > 1:226793/1‑139 (MQ=255) ccccACACCGCCGCCGGAACGCGCGGCGTCACGGACGGATATTCCGGTTTGTTGGCGTCAACTTCGACGCGAACCGTAATCCAACACTTAGCGCCCTTCTCCATCGACAGCACGTAGTAAACGGCGTAATGACCATTCa > 2:757761/1‑139 (MQ=255) ccccACACCGCCGCCGGAACGCGCGGCGTCACGGACGGATATTCCGGTTTGTTGGCGTCAACTTCGACGCGAACCGTAATCCAACACTTAGCGCCCTTCTCCATCGACAGCACGTAGTAAACGGCGTAATGACCATTCa > 1:990925/1‑139 (MQ=255) cgcgGCGTCACGGACGGATATTCCGGTTTGTTGGCGTCAACTTCGACGCGAACCGTAATCCAACACTTAGCTCTCTTCTCCATCGACAGCACGTAGTAAACGGCGTAATGACCATTCAGTTTGCGTTCGTCGTTACCaa < 1:677281/139‑1 (MQ=255) cACGGACGGATATTCCGGTTTGTTGGCGTCAACTTCGACGCGAACCGTAATCCAACACTTAGCGCCCTTCTCCATCGACAGCACGTAGTAAACGGCGTAATGACCATTCAGTTTGCGTTCGTCGTTACCAAACAGCAcc > 1:13498/1‑139 (MQ=255) ggaTATTCCGGTTTGTTGGCGTCAACTTCGACGCGAACCGTAATCCAACACTTAGCGCCCTTCTCCATCGACAGc < 1:802639/75‑1 (MQ=255) ggaTATTCCGGTTTGTTGGCGTCAACTTCGACGCGAACCGTAATCCAACACTTAGCGCCCTTCTCCATCGACAGc > 2:802639/1‑75 (MQ=255) atatTCCGGTTTGTTGGCGTCAACTTCGACGCGAACCGTAATCCAACACTTAGCGCCCTTCTCCATCGACAGCACGTAGTAAACGGCGTAATGACCATTCAGTTTGCGTTCGTCGTTACCAAACAGCACCGAcagccag > 1:459463/1‑139 (MQ=255) ccGGTTTGTTGGCGTCAACTTCGACGCGAACCGTAATCCAACACTTAGCGCCCTTCTCCATCGACAGCACGTAGTAAACGGCGTAATGACCATTCAGTTTGCGTTCGTCGTTACCAAACAGCACCGACAGCCAGCCAcc < 1:908080/139‑1 (MQ=255) ccGGTTTGTTGGCGTCAACTTCGACGCGAACCGTAATCCAACACTTAGCGCCCTTCTCCATCGACAGCACGTAGTAAACGGCGTAATGACCATTCAGTTTGCGTTCGTCGTTACCAAACAGCACCGACAGCCAGCCAcc < 1:757761/139‑1 (MQ=255) ccGGTTTGTTGGCGTCAACTTCGACGCGAACCGTAATCCAACACTTAGCGCCCTTCTCCATCGACAGCACGTAGTAAACGGCGTAATGACCATTCAGTTTGCGTTCGTCGTTACCAAACAGCACCGACAGCCAGCCAcc < 2:226793/139‑1 (MQ=255) ccGGTTTGTTGGCGTCAACTTCGACGCGAACCGTAATCCAACACTTAGCGCCCTTCTCCATCGACAGCACGTAGTAAACGGCGTAATGACCATTCAGTTTGCGTTCGTCGTTACCAAACAGCACCGACAGCCAGCCAcc < 2:459463/139‑1 (MQ=255) tCAACTTCGACGCGAACCGTAATCCAACACTTAGCGCCCTTCTCCATCGACAGCACGTAGTAAACGGCGTAATGACCATTCAGTTTGCGTTCGTCGTTACCAAACAGCACCGACAGCCAGCCACCCTGTTTGTAGTaaa < 1:371468/139‑1 (MQ=255) cGACGCGAACCGTAATCCAACACTTAGCGCCCTTCTCCATCGACAGCACGTAGTAAACGGCGTAATGACCATTCAGTTTGCGTtc > 1:670757/1‑85 (MQ=255) cGACGCGAACCGTAATCCAACACTTAGCGCCCTTCTCCATCGACAGCACGTAGTAAACGGCGTAATGACCATTCAGTTTGCGTtc < 2:670757/85‑1 (MQ=255) cgcgAACCGTAATCCAACACTTAGCGCCCTTCTCCATCGACAGCACGTAGTAAACGGCGTAATGACCATTCAGTTTGCg > 1:165345/1‑79 (MQ=255) cgcgAACCGTAATCCAACACTTAGCGCCCTTCTCCATCGACAGCACGTAGTAAACGGCGTAATGACCATTCAGTTTGCg < 2:165345/79‑1 (MQ=255) gAACCGTAATCCAACACTTAGCGCCCTTCTCCATCGACAGCACGTAGTAAACGGCGTAATGACCATTCAGTTTGCGTTCGTCGTTACCAAACAGCACCGACAGCCAGCCACCCTGTTTGTAGTAAAGAAACTCcaccac < 2:13498/139‑1 (MQ=255) aCCGTAATCCAACACTTAGCGCCCTTCTCCATCGACAGCACGTAGTAAACGGCGTAATGACCATTCAGTTTGCGTTCGTCGTTACCAAACAGCACCGACAGCCAGCCACCCTGTTTGTAGTAAAGAAACTCCACCACtt > 2:987273/1‑139 (MQ=255) aaTCCAACACTTAGCGCCCTTCTCCATCGACAGCACGTAGTAAACGGCGTAATGACCATTCAGTTTGCGTTCGTCGTTACCAAACAGCACCGACAGCCAGCCACCCTGTTTGTAGTAAAGAAACTCCACCACTTCCGGc > 1:907892/1‑139 (MQ=255) tAGCGCCCTTCTCCATCGACAGCACGTAGTAAACGGCGTAATGACCATTCAGTTTGCGTTCGTCGTTACCAAACAGCACCGACAGCCAGCCACCCTGTTTGTAGTAAAGAAACTCCACCACTTCCGGCAGGTAGTTCAc < 2:645078/139‑1 (MQ=255) tAGCGCCCTTCTCCATCGACAGCACGTAGTAAACGGCGTAATGACCATTCAGTTTGCGTTCGTCGTTACCAAACAGCACCGACAGCCAGCCACCCTGTTTGTAGTAAAGAAACTCCACCACTTCCGGCAGGTAGTTCAc < 2:990925/139‑1 (MQ=255) | GAATCAAACCGTACATATCGCGCACTTCACGCTCGCCCCACACCGCCGCCGGAACGCGCGGCGTCACGGACGGATATTCCGGTTTGTTGGCGTCAACTTCGACGCGAACCGTAATCCAACACTTAGTGCCCTTCTCCATCGACAGCACGTAGTAAACGGCGTAATGACCATTCAGTTTGCGTTCGTCGTTACCAAACAGCACCGACAGCCAGCCACCCTGTTTGTAGTAAAGAAACTCCACCACTTCCGGCAGGTAGTTCAC > NC_000913/2846096‑2846357 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |