Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,851,873	G→A	*291* (TAG→TAA)  G4S (GGC→AGC)	hypB → hypC →	GTP hydrolase involved in nickel liganding into hydrogenases hydrogenase maturation protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,851,873	0	G	A	100.0%	47.5 / NA	17	*291* (TAG→TAA)  G4S (GGC→AGC)	hypB hypC	GTP hydrolase involved in nickel liganding into hydrogenases hydrogenase maturation protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (11/6);  total (11/6)

ACGTTGAAAAGTGCATCGCCTGCGCCCGCGAAGTCAATCCAGAAATTGAAATCATCCTTATTTCCGCCACCAGCGGCGAAGGGATGGACCAGTGGCTGAACTGGCTGGAGACACAGCGATGTGCATAGGCGTTCCCGGCCAGATCCGCACCATTGACGGCAACCAGGCGAAAGTCGACGTCTGCGGCATTCAGCGCGATGTCGATTTAACGTTAGTCGGCAGCTGCGATGAAAACGGTCAGCCGCGCG  >  NC_000913/2851746‑2851993                                                                                                                                |                                                                                                                         aCGTTGAAAAGTGCATCGCCTGCGCCCGCGAAGTCAATCCAGAAATTGAAATCATCCTTATTTCCGCCACCAGCGGCGAAGGGATGGACCAGTGGCTGAACTGGCTGGAGACACAGCGATGTGCATAAGCGTTCCCGGc                                                                                                               >  1:507676/1‑139 (MQ=255)  cGTTGAAAAGTGCATCGCCTGCGCCCGCGAAGTCAATCCAGAAATTGAAATCATCCTTATTTCCGCCACCAGCGGCGAAGGGATGGACCAGTGGCTGAACTGGCTGGAGACACAGCGATGTGCATAAGCGTTCCCGGcc                                                                                                              <  2:507676/139‑1 (MQ=255)             gCATCGCCTGCGCCCGCGAAGTCAATCCAGAAATTGAAATCATCCTTATTTCCGCCACCAGCGGCGAAGGGATGGACCAGTGGCTGAACTGGCTGGAGACACAGCGATGTGCATAAGCGTTCCCGGCCAGATCCGCAcc                                                                                                   >  2:218160/1‑139 (MQ=255)                     tGCGCCCGCGAAGTCAATCCAGAAATTGAAATCATCCTTATTTCCGCCACCAGCGGCGAAGGGATGGACCAGTGGCTGAACTGGCTGGAGACACAGCGATGTGCATAAGCGTTCCCGGCCAGATCCGCACCATTGACgg                                                                                           >  2:896408/1‑139 (MQ=255)                         cccGCGAAGTCAATCCAGAAATTGAAATCATCCTTATTTCCGCCACCAGCGGCGAAGGGATGGACCAGTGGCTGAACTGGCTGGAGACACAGCGATGTGCATAAGCGTTCCCGGCCAGATCCGCACCATTGACGGCAAc                                                                                       >  2:784635/1‑139 (MQ=255)                             cgAAGTCAATCCAGAAATTGAAATCATCCTTATTTCCGCCACCAGCGGCGAAGGGATGGACCAGTGGCTGAACTGGCTGGAGACACAGCGATGTGCATAAGCGTTCCCGGCCAGATCCGCACCATTGACGGCAACCAgg                                                                                   >  2:372161/1‑139 (MQ=255)                             cgAAGTCAATCCAGAAATTGAAATCATCCTTATCTCCGCCACCAGCGGCGAAGGGATGGACCAGTGGCTGAACTGGCTGGAGACACAGCGATGTGCATAAGCGTTCCCGGCCAGATCCGCACCATTGACGGCAACCAgg                                                                                   <  1:847170/139‑1 (MQ=255)                                                 aaaTCATCCTTATTTCCGCCACCAGCGGCGAAGGGATGGACCAGTGGCTGAACTGGCTGGAGACACAGCGATGTGCATAAGCGTTCCCGGCCAGATCCGCACCATTGACGGCAACCAGGCGAAAGTCGACGTCTGCGGc                                                               >  2:418838/1‑139 (MQ=255)                                                              ttCCGCCACCAGCGGCGAAGGGATGGACCAGTGGCTGAACTGGCTGGAGACACAGCGATGTGCATAAGCGTTCCCGGCCAGATCCGCACCATTGACGGCAACCAGGCGAAAGTCGACGTCTGCGGCATTCAGCGCGATg                                                  <  1:418838/139‑1 (MQ=255)                                                                  gCCACCAGCGGCGAAGGGATGGACCAGTGGCTGAACTGGCTGGAGACACAGCGATGTGCATAAGCGTTCCCGGCCAGATCCGCACCAtt                                                                                                <  2:387015/89‑1 (MQ=255)                                                                  gCCACCAGCGGCGAAGGGATGGACCAGTGGCTGAACTGGCTGGAGACACAGCGATGTGCATAAGCGTTCCCGGCCAGATCCGCACCAtt                                                                                                >  1:387015/1‑89 (MQ=255)                                                                  gCCACCAGCGGCGAAGGGATGGACCAGTGGCTGAACTGGCTGGAGACACAGCGATGTGCATAAGCGTTCCCGGCCAGATCCGCACCATTGACGGCAACCAGGCGAAAGTCGACGTCTGCGGCATTCAGCGCGATGTCGa                                              >  2:724747/1‑139 (MQ=255)                                                                  gCCACCAGCGGCGAAGGGATGGACCAGTGGCTGAACTGGCTGGAGACACAGCGATGTGCATAAGCGTTCCCGGCCAGATCCGCACCATTGACGGAAACCAGGCGAAAGTCGACGTCTGTGGCATTCAGCGCGATGTcta                                              >  1:700064/1‑137 (MQ=255)                                                                    caccaGCGGCGAAGGGATGGACCAGTGGCTGAACTGGCTGGAGACACAGCGATGTGCATAAGCGTTCCCGGCCAGATCCGCACCATTGACGGCAACCAGGCGAAAGTCGACGTCTGCGGCATTCAGCGCGATGTCGAtt                                            <  1:724747/139‑1 (MQ=255)                                                                      ccaGCGGCGAAGGGATGGACCAGTGGCTGAACTGGCTGGAGACACAGCGATGTGCATAAGCGTTCCCGGCCAGATCCGCACCATTGACGGCAACCAGGCGAAAGTCGACGTCTGCGGCATTCAGCGCGATGTCGATTTa                                          >  2:73105/1‑139 (MQ=255)                                                                                     tGGACCAGTGGCTGAACTGGCTGGAGACACAGCGATGTGCATAAGCGTTCCCGGCCAGATCCGCACCATTGACGGCAACCAGGCGAAAGTCGACGTCTGCGGCATTCAGCGCGATGTCGATTTAACGTTAGTCGGCAGc                           >  2:941007/1‑139 (MQ=255)                                                                                                              gaCACAGCGATGTGCATAAGCGTTCCCGGCCAGATCCGCACCATTGACGGCAACCAGGCGAAAGTCGACGTCTGCGGCATTCAGCGCGATGTCGATTTAACGTTAGTCGGCAGCTGCGATGAAAACGGTCAGCcgcgcg  <  2:166394/139‑1 (MQ=255)                                                                                                                                |                                                                                                                         ACGTTGAAAAGTGCATCGCCTGCGCCCGCGAAGTCAATCCAGAAATTGAAATCATCCTTATTTCCGCCACCAGCGGCGAAGGGATGGACCAGTGGCTGAACTGGCTGGAGACACAGCGATGTGCATAGGCGTTCCCGGCCAGATCCGCACCATTGACGGCAACCAGGCGAAAGTCGACGTCTGCGGCATTCAGCGCGATGTCGATTTAACGTTAGTCGGCAGCTGCGATGAAAACGGTCAGCCGCGCG  >  NC_000913/2851746‑2851993

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑