Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,893,928 G→A *424* (TAG→TAA
A4T (GCC→ACC) 
ygcN →
ygcO →
putative oxidoreductase
putative 4Fe‑4S cluster‑containing protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,893,9280GA100.0% 107.2 / NA 34*424* (TAG→TAA
A4T (GCC→ACC) 
ygcN
ygcO
putative oxidoreductase
putative 4Fe‑4S cluster‑containing protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (20/14);  total (20/14)

TTTCCCGCGATTTATGGGATCAGGGTGATAAACCTGTTCCACCGCTGCGCCAGTTATTCTGGCATCATTTACGTCGTCACGGCCTGTGGCATCTGGCGGGCGATGTTATCAGGAGTCTGCGATGTCTGTAGCCCGTAATCTCTGGCGCGTTGCTGATGCGCCGCACATTGTTCCGGCTGACTCCGTTGAGCGCCAGACGGCAGAACGGTTGATTAACGCCTGTCCGGCAGGTCTTTTTTCGCTCACACCGGAAGGTA  >  NC_000913/2893798‑2894054
                                                                                                                                  |                                                                                                                              
tttCCCGCGATTTATGGGATCAGGGTGATAAACCTGTTCCACCGCTGCGCCAGTTATTCTGGCATCATTTACGTCGTCACGGCCTGTGGCATCTGGCGGGCGATGTTATCAGGAGTCTGCAATGTCTGTAACCCGTAAt                                                                                                                        >  1:615865/1‑139 (MQ=255)
      gcgATTTATGGGATCAGGGTGATAAACCTGTTCCACCGCTGCGCCAGTTATTCTGGCATCATTTACGTCGTCACGGCCTGTGGCATCTGGCGGGCGATGTTATCAGGAGTCTGCGATGTCTGTAACCCGTAATCTCTgg                                                                                                                  >  1:606617/1‑139 (MQ=255)
           ttATGGGATCAGGGTGATAAACCTGTTCCACCGCTGCGCCAGTTATTCTGGCATCATTTACGTCGTCACGGCCTGTGGCATCTGGCGGGCGATGTTATCAGGAGTCTGCAATGTCTGTAACCCGTAATCTCTGGCGCGt                                                                                                             <  2:615865/139‑1 (MQ=255)
                  aTCAGGGTGATAAACCTGTTCCACCGCTGCGCCAGTTATTCTGGCATCATTTACGTCGTCACGGCCTGTGGCATCTGGCGGGCGATGTTATCAGGAGTCTGCGATGTCTGTAACCCGTAATCTCTGGCGCGTTGCTGAt                                                                                                      >  1:885760/1‑139 (MQ=255)
                    cAGGGTGATAAACCTGTTCCACCGCTGCGCCAGTTATTCTGGCATCATTTACGTCGTCACGGCCTGTGGCATCTGGCGGGCGATGTTATCAGGAGTCTGCGATGTCTGTAACCCGTAATCTCTGGCGCGTTGCTGATgc                                                                                                    >  1:941165/1‑139 (MQ=255)
                           aTAAACCTGTTCCACCGCTGCGCCAGTTATTCTGGCATCATTTACGTCGTCACGGCCTGTGGCATCTGGCGGGCGATGTTATCAGGAGTCTGCGATGTCTGTAACCCGTAATCTCTGGCGCGTTGCTGATGCGCCGatt                                                                                             >  1:390916‑M1/1‑136 (MQ=255)
                                 cTGTTCCACCGCTGCGCCAGTTATTCTGGCATCATTTACGTCGTCACGGCCTGTGGCATCTGGCGGGCGATGTTATCAGGAGTCTGCGATGTCTGTAACCCGTAATCTCTGGCGCGTTGCTGATGCGCCGattgtt                                                                                          <  2:805600‑M1/136‑7 (MQ=255)
                                 cTGTTCCACCGCTGCGCCAGTTATTCTGGCATCATTTACGTCGTCACGGCCTGTGGCATCTGGCGGGCGATGTTATCAGGAGTCTGCGATGTCTGTAACCCGTAATCTCTGGCGCGTTGCTGATGCGCCGattgtt                                                                                          >  1:805600‑M1/1‑130 (MQ=255)
                                         ccGCTGCGCCAGTTATTCTGGCATCATTTACGTCGTCACGGCCTGTGGCATCTGGCGGGCGATGTTATCAGGAGTCTGCGATGTCTGTAACCCGTAATCTCTGGCGCGTTGCTGATGCGCCGattgttccggctgactc                                                                               >  1:309932‑M1/1‑122 (MQ=255)
                                              gcgcCAGTTATTCTGGCATCATTTACGTCGTCACGGCCTGTGGCATCTGGCGGGCGATGTTATCAGGAGTCTGCGATGTCTGTAACCCGTAATCTCTg                                                                                                                   <  2:611930/98‑1 (MQ=255)
                                              gcgcCAGTTATTCTGGCATCATTTACGTCGTCACGGCCTGTGGCATCTGGCGGGCGATGTTATCAGGAGTCTGCGATGTCTGTAACCCGTAATCTCTGGCGCGTTGCTGATGCGCCGattgttccggctgactccg                                                                             <  1:834292‑M1/136‑20 (MQ=255)
                                              gcgcCAGTTATTCTGGCATCATTTACGTCGTCACGGCCTGTGGCATCTGGCGGGCGATGTTATCAGGAGTCTGCGATGTCTGTAACCCGTAATCTCTGGCGCGTTGCTGATGCGCCGattgttccggctgactccg                                                                             >  2:834292‑M1/1‑117 (MQ=255)
                                              gcgcCAGTTATTCTGGCATCATTTACGTCGTCACGGCCTGTGGCATCTGGCGGGCGATGTTATCAGGAGTCTGCGATGTATGTAACCCGTAATCTCTg                                                                                                                   >  1:611930/1‑98 (MQ=255)
                                                    gTTATTCTGGCATCATTTACGTCGTCACGGCCTGTGGCATCTGGCGGGCGATGTTATCAGGAGTCTGCGATGTCTGTAACCCGTAATCTCTGGCGCGTTGCTGATGCGCCGattgttccggctgactccgttgagcgcc                                                                    >  2:396673‑M1/1‑111 (MQ=255)
                                                                            tcACGGCCTGTGGCATCTGGCGGGCGATGTTATCAGGAGTCTGCGATGTCTGTAACCCGTAATCTCTGGCGCGTTGCTGATGCGCCGattgttccggctgactccgttgagcgccagacggcagaacggttgattaacg                                            >  1:417080‑M1/1‑87 (MQ=255)
                                                                                      tgGCATCTGGCGGGCGATGTTATCAGGAGTCTGCGATGTCTGTAACCCGTAATCTCTGGCGCGTTGCTGATGCGCCGattgttccggctgactccgttgagcgccagacggcagaacggttgattaacgcctgtccggc                                  >  2:416153‑M1/1‑77 (MQ=255)
                                                                                              ggcgggcgATGTTATCAGGAGTCTGCGATGTCTGTAACCCGTAATCTCTGGCGCGTTGCTGATGCGCCGattgttccggctgactccgttgagcgccagacggcagaacggttgattaacgcctgtccggcaggtcttt                          >  2:725800‑M1/1‑69 (MQ=255)
                                                                                                  ggcgATGTTATCAGGAGTCTGCGATGTCTGTAAcc                                                                                                                              <  2:853042/35‑1 (MQ=255)
                                                                                                  ggcgATGTTATCAGGAGTCTGCGATGTCTGTAAcc                                                                                                                              >  1:853042/1‑35 (MQ=255)
                                                                                                  ggcgATGTTATCAGGAGTCTGCGATGTCTGTAACCCGTAATCTCTg                                                                                                                   >  2:307986/1‑46 (MQ=255)
                                                                                                  ggcgATGTTATCAGGAGTCTGCGATGTCTGTAACCCGTAATCTCTg                                                                                                                   <  1:307986/46‑1 (MQ=255)
                                                                                                   gcgATGTTATCAGGAGTCTGCGATGTCTGTAACCCGTAATCTCTGGCGCGTTGCTGATGCGCCGattgttccggctgactccgttgagcgccagacggcagaacggttgattaacgcctgtccggcaggtcttttttcg                     <  2:390916‑M1/139‑76 (MQ=255)
                                                                                                           aTCAGGAGTCTGCGATGTCTGTAACCCGTAATCTCTGGCGCGTTGCTGATGCGCCGattgttccggctgactccgttgagcgccagacggcagaacggttgattaacgcctgtccggcaggtcttttttcactcacacc             <  2:941165‑M1/139‑84 (MQ=255)
                                                                                                             cAGGAGTCTGCGATGTCTGTAACCCGTAATCTCTGGCGCGTTGCTGATGCGCCGattgttccggctgactccgttgagcgccagacggcagaacggttgattaacgcctgtccggcaggtcttttttcgctcacaccgg           <  1:725800‑M1/139‑86 (MQ=255)
                                                                                                                 aGTCTGCGATGTCTGTAACCCGTAATCTCTGGCGCGTTGCTGATGCGCCGattgttccggctgactccgttgagcgccagacggcagaa                                                         <  2:980080‑M1/89‑40 (MQ=255)
                                                                                                                 aGTCTGCGATGTCTGTAACCCGTAATCTCTGGCGCGTTGCTGATGCGCCGattgttccggctgactccgttgagcgccagacggcagaa                                                         >  1:980080‑M1/1‑50 (MQ=255)
                                                                                                                  gTCTGCGATGTCTGTAACCCGTAATCTCTGGCGCGTTGCTGATGCGCCGattgttccggctgactccgttgagcgccagacggcagaacggttgattaacgcctgtccggcaggtcttttttcgctcacaccggaaggt      <  2:885760‑M1/139‑91 (MQ=255)
                                                                                                                   tCTGCGATGTCTGTAACCCGTAATCTCTGGCGCGTTGCTGATGCGCCGattgttccggctgactccgttgagcgccagacggcagaacggttgattaacgcctgtccggcaggtcttttttcgctcacaccggaaggta     >  2:581511‑M1/1‑48 (MQ=255)
                                                                                                                   tCTGCGATGTCTGTAACCCGTAATCTCTGGCGCGTTGCTGATGCGCCGattgttccggctgact                                                                                <  1:897022‑M1/64‑17 (MQ=255)
                                                                                                                   tCTGCGATGTCTGTAACCCGTAATCTCTGGCGCGTTGCTGATGCGCCGattgttccggctgact                                                                                >  2:897022‑M1/1‑48 (MQ=255)
                                                                                                                    cTGCGATGTCTGTAACCCGTAATCTCTGGCGCGTTGCTGATGCGCCGattgttccggctgactccgttgagcgccagacggcagaacggttgattaacgcctgtccggcaggtcttttttcgctcacaccggaaggtaa    <  1:416153‑M1/139‑93 (MQ=255)
                                                                                                                      gCGATGTCTGTAACCCGTAATCTCTGGCGCGTTGCTGATGCGCCGattgttccggctgactccgttgagcgccagccggcagaacggttgattaacgcctgtccggcaggtcttttttcgctcacaccggaaggtaact  >  1:815911‑M1/1‑45 (MQ=255)
                                                                                                                             cTGTAACCCGTAATCTCTGGCGCGTTGCTGATGCGCCGattgttccggctgactccgttgagcgccagacggcagaacggttgattaacgcctgtccggcaggtcttttttcgctcacaccggaaggtaact  <  2:371051‑M1/132‑95 (MQ=255)
                                                                                                                             cTGTAACCCGTAATCTCTGGCGCGTTGCTGATGCGCCGattgttccggctgactccgttgagcgccagacggcagaacggttgattaacgcctgtccggcaggtcttttttcgctcacaccggaaggtaact  >  1:371051‑M1/1‑38 (MQ=255)
                                                                                                                                  |                                                                                                                              
TTTCCCGCGATTTATGGGATCAGGGTGATAAACCTGTTCCACCGCTGCGCCAGTTATTCTGGCATCATTTACGTCGTCACGGCCTGTGGCATCTGGCGGGCGATGTTATCAGGAGTCTGCGATGTCTGTAGCCCGTAATCTCTGGCGCGTTGCTGATGCGCCGCACATTGTTCCGGCTGACTCCGTTGAGCGCCAGACGGCAGAACGGTTGATTAACGCCTGTCCGGCAGGTCTTTTTTCGCTCACACCGGAAGGTA  >  NC_000913/2893798‑2894054

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: