Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 284,869 C→T P557S (CCG→TCG)  yagF → CP4‑6 prophage, dehydratase family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913284,8690CT100.0% 88.5 / NA 29P557S (CCG→TCG) yagFCP4‑6 prophage, dehydratase family protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (13/16);  total (13/16)

CTCCGCGCTAAAGCATATCTCGTGGGGCAAGACGGTGTCGCTCATCACCGATGCGCGCTTCTCGGGCGTGTCGACGGGCGCCTGCTTCGGCCACGTGTCGCCGGAGGCGCTGGCGGGCGGGCCGATTGGCAAGCTGCGCGATAACGACATCATCGAGATTGCCGTGGATCGTCTGACGTTAACTGGCAGCGTGAACTTCATCGGCACCGCGGACAACCCGCTGACGCCGGAAGAGGG  >  NC_000913/284748‑284984
                                                                                                                         |                                                                                                                   
cTCCGCGCTAAAGCATATCTCGTGGGGCAAGACGGTGTCGCTCATCACCGATGCGCGCTTCTCGGGCGTGTCGACGGGCGCCTGCTTCGGCCACGTGTCGCCGGAGGCGCTGGCGGGCGGGTCGATTGGCAAGCTgcgc                                                                                                    <  1:702486/139‑1 (MQ=255)
     cgcTAAAGCATATCTCGTGGGGCAAGACGGTGTCGCTCATCACCGATGCGCGCTTCTCGGGCGTGTCGACGGGCGCCTGCTTCGGCCACGTGTCGCCGGAGGCGCTGGCGGGCGGGTCGATTGGCAAGCTGCGCGATaa                                                                                               >  1:147803/1‑139 (MQ=255)
                    cGTGGGGCAAGACGGTGTCGCTCATCACCGATGCGCGCTTCTCGGGCGTGTCGACGGGCGCCTGCTTCGGCCACGTGTCGCCGGAGGCGCTGGCGGGCGGGTCGATTGGCAAGCTGCGCGATAACGACATCATCgaga                                                                                 >  1:500706/1‑138 (MQ=255)
                    cGTGGGGCAAGACGGTGTCGCTCATCACCGATGCGCGCTTCTCGGGCGTGTCGACGGGCGCCTGCTTCGGCCACGTGTCGCCGGAGGCGCTGGCGGGCGGGTCGATTGGCAAGCTGCGCGATAACGACATCATCgaga                                                                                 <  2:500706/138‑1 (MQ=255)
                      tGGGGCAAGACGGTGTCGCTCATCACCGATGCGCGCTTCTCGGGCGTGTCGACGGGCGCCTGCTTCGGCCACGTGTCGCCGGAGGCGCTGGCGGGCGGGTCGATTGGCa                                                                                                            >  1:524821/1‑109 (MQ=255)
                      tGGGGCAAGACGGTGTCGCTCATCACCGATGCGCGCTTCTCGGGCGTGTCGACGGGCGCCTGCTTCGGCCACGTGTCGCCGGAGGCGCTGGCGGGCGGGTCGATTGGCa                                                                                                            <  2:524821/109‑1 (MQ=255)
                      tGGGGCAAGACGGTGTCGCTCATCACCGATGCGCGCTTCTCGGGCGTGTCGACGGGCGCCTGCTTCGGCCACGTGTCGCCGGAGGCGCTGGCGGGCGGGTCGATTGGCAAGCTGCGCGATAACGACATCATCGAGATTg                                                                              >  1:569174/1‑139 (MQ=255)
                       ggggCAAGACGGTGTCGCTCATCACCGATGCGCGCTTCTCGGGCGTGTCGACGGGCGCCTGCTTCGGCCACGTGTCGCCGGAGGCGCTGGCGGGCGGGTCGATTGGCAAGCTGCGCGATAACGACATCATCGAGATTGc                                                                             >  1:357728/1‑139 (MQ=255)
                         ggCAAGACGGTGTCGCTCATCACCGATGCGCGCTTCTCGGGCGTGTCGACGGGCGCCTGCTTCGGCCACGTGTCGCCGGAGGCGCTGGCGGGCGGGTCGATTGGCAAGCTGCGCGATAACGACATCATCGAGATTGCCg                                                                           <  2:147803/139‑1 (MQ=255)
                          gCAAGACGGTGTCGCTCATCACCGATGCGCGCTTCTCGGGCGTGTCGACGGGCGCCTGCTTCGGCCACGTGTCGCCGGAGGCGCTGGCGGGCGGGTCGATTGGCAAGCTGCGCGATAACGACATCATCGAGATTGCCGt                                                                          >  1:63138/1‑139 (MQ=255)
                                   tgtCGCTCATCACCGATGCGCGCTTCTCGGGCGTGTCGACGGGCGCCTGCTTCGGCCACGTGTCGCCGGAGGCGCTGGCGGGCGGGTCGATTGGCAAGCTGCGCGATAACGAcatcat                                                                                      <  2:863252/118‑1 (MQ=255)
                                   tgtCGCTCATCACCGATGCGCGCTTCTCGGGCGTGTCGACGGGCGCCTGCTTCGGCCACGTGTCGCCGGAGGCGCTGGCGGGCGGGTCGATTGGCAAGCTGCGCGATAACGAcatcat                                                                                      >  1:863252/1‑118 (MQ=255)
                                             caCCGATGCGCGCTTCTCGGGCGTGTCGACGGGCGCCTGCTTCGGCCACGTGTCGCCGGAGGCGCTGGCGGGCGGGTCGATTGGCAAGCTGCGCGATAACGACATCATCGAGATTGCCGTGGATCGTCTGACGTTAACt                                                       >  1:87116/1‑139 (MQ=255)
                                                   tGCGCGCTTCTCGGGCGTGTCGACGGGCGCCTGCTTCGGCCACGTGTCGCCGGAGGCGCTGGCGGGCGGGTCGATTGGCAAGCTGCGCGAt                                                                                                 >  2:929947/1‑91 (MQ=255)
                                                   tGCGCGCTTCTCGGGCGTGTCGACGGGCGCCTGCTTCGGCCACGTGTCGCCGGAGGCGCTGGCGGGCGGGTCGATTGGCAAGCTGCGCGAt                                                                                                 <  1:929947/91‑1 (MQ=255)
                                                      gcgcTTCTCGGGCGTGTCGACGGGCGCCTGCTTCGGCCACGTGTCGCCGGAGGCGCTGGCGGGCGGGTCGATTGGCAAGCTGCGCGATAACGACATCATCGAGATTGCCGTGGATCGTCTGACGTTAACTGGCAGCGTg                                              <  2:569174/139‑1 (MQ=255)
                                                                    tgtCGACGGGCGCCTGCTTCGGCCACGTGTCGCCGGAGGCGCTGGCGGGCGGGTCGATTGGCAAGCTGcgcg                                                                                                   >  1:894055/1‑72 (MQ=255)
                                                                    tgtCGACGGGCGCCTGCTTCGGCCACGTGTCGCCGGAGGCGCTGGCGGGCGGGTCGATTGGCAAGCTGcgcg                                                                                                   <  2:894055/72‑1 (MQ=255)
                                                                    tgtCGACGGGCGCCTGCTTCGGCCACGTGTCGCCGGAGGCGCTGGCGGGCGGGTCGATTGGCAAGCTGCGCGATAACGACATCATCGAGATTGCCGTGGATCGTCTGACGTTAACTgg                                                     >  2:134536/1‑118 (MQ=255)
                                                                    tgtCGACGGGCGCCTGCTTCGGCCACGTGTCGCCGGAGGCGCTGGCGGGCGGGTCGATTGGCAAGCTGCGCGATAACGACATCATCGAGATTGCCGTGGATCGTCTGACGTTAACTgg                                                     <  1:134536/118‑1 (MQ=255)
                                                                      tCGACGGGCGCCTGCTTCGGCCACGTGTCGCCGGAGGCGCTGGCGGGCGGGTCGATTGGCAAGCTGCGCGATAACg                                                                                             >  2:393802/1‑76 (MQ=255)
                                                                      tCGACGGGCGCCTGCTTCGGCCACGTGTCGCCGGAGGCGCTGGCGGGCGGGTCGATTGGCAAGCTGCGCGATAACg                                                                                             <  1:393802/76‑1 (MQ=255)
                                                                          cGGGCGCCTGCTTCGGCCACGTGTCGCCGGAGGCGCTGGCGGGCGGGTCGATTGGCAAGCTGCGCGATAACGACATCATCGAGATTGCCGTGGATCGTCTGACGTTAACTGGCAGCGTGAACTTCATCGGCACCGCGGa                          <  2:357728/139‑1 (MQ=255)
                                                                                 cTGCTTCGGCCACGTGTCGCCGGAGGCGCTGGCGGGCGGGTCGATTGGCAAGCTGCGCGATAACGACATCATCGAGATTGCCGTGGATCGTCTGACGTTAACTGGCAGCGTGAACTTCATCGGCACCGCGGACAACCCg                   <  2:59483/139‑1 (MQ=255)
                                                                                   gCTTCGGCCACGTGTCGCCGGAGGCGCTGGCGGGCGGGTCGATTGGCAAGCTGCGCGATAACGACATCATCGAGATTGCCGTGGATCGTCTGACGTTAACTGGCAGCGTGAACTTCATCGGCACCGCGGACAACCCGCt                 <  2:763959/139‑1 (MQ=255)
                                                                                       cGGCCACGTGTCGCCGGAGGCGCTGGCGGGCGGGTCGATTGGCAAGCTGCGCGATAACGACATCATCGAGATTGCCGTGGATCGTCTGACGTTAACTGGCAGCGTGAACTTCATCGGCACCGCGGACAACCCGCTGACg             <  1:631150/139‑1 (MQ=255)
                                                                                        ggCCACGTGTCGCCGGAGGCGCTGGCGGGCGGGTCGATTGGCAAGCTGCGCGAt                                                                                                 <  1:773725/54‑1 (MQ=255)
                                                                                        ggCCACGTGTCGCCGGAGGCGCTGGCGGGCGGGTCGATTGGCAAGCTGCGCGAt                                                                                                 >  2:773725/1‑54 (MQ=255)
                                                                                                  cGCCGGAGGCGCTGGCGGGCGGGTCGATTGGCAAGCTGCGCGATAACGACATCATCGAGATTGCCGTGGATCGTCTGACGTTAACTGGCAGCGTGAACTTCATCGGCACCGCGGACAACCCGCTGACGCCGGAAGAggg  <  2:374035/139‑1 (MQ=255)
                                                                                                                         |                                                                                                                   
CTCCGCGCTAAAGCATATCTCGTGGGGCAAGACGGTGTCGCTCATCACCGATGCGCGCTTCTCGGGCGTGTCGACGGGCGCCTGCTTCGGCCACGTGTCGCCGGAGGCGCTGGCGGGCGGGCCGATTGGCAAGCTGCGCGATAACGACATCATCGAGATTGCCGTGGATCGTCTGACGTTAACTGGCAGCGTGAACTTCATCGGCACCGCGGACAACCCGCTGACGCCGGAAGAGGG  >  NC_000913/284748‑284984

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: