Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	287,977	A→G	M397V (ATG→GTG)	yagH →	CP4‑6 prophage, putative xylosidase/arabinosidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	287,977	0	A	G	100.0%	85.9 / NA	25	M397V (ATG→GTG)	yagH	CP4‑6 prophage, putative xylosidase/arabinosidase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (12/13);  total (12/13)

GGCTTCTTACGGCTCTATGGCAACGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGATGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTGCTACTACAACAGCAAAAACTGGAGCTACTGCTTTGTGGACTACGAGGAGGGACAGGGTAGAACCATCAAAGTTATCC  >  NC_000913/287875‑288100                                                                                                       |                                                                                                                            ggCTTCTTACGGCTCTATGGCAACGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGGTGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCgg                                                                                         >  2:716132/1‑139 (MQ=255) ggCTTCTTACGGCTCTATGGCAACGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGGTGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCgg                                                                                         >  2:400051/1‑139 (MQ=255)       ttACGGCTCTATGGCAACGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGGTGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGa                                                                                   <  2:527389/139‑1 (MQ=255)         aCGGCTCTATGGCAACGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGGTGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGAcc                                                                                 <  2:981385/139‑1 (MQ=255)         aCGGCTCTATGGCAACGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGGTGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGAcc                                                                                 <  1:66720/139‑1 (MQ=255)                tATGGCAACGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGGTGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTGctact                                                                          >  2:448024/1‑139 (MQ=255)                 aTGGCAACGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGGTGGAGTTCTCGCCGGTGCACTTCcagca                                                                                                 <  2:336549/115‑1 (MQ=255)                 aTGGCAACGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGGTGGAGTTCTCGCCGGTGCACTTCcagca                                                                                                 >  1:336549/1‑115 (MQ=255)                  tGGCAACGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGGTGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTGCtactac                                                                        >  1:529861/1‑139 (MQ=255)                       aCGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGGTGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGa                                                                                   >  1:767320/1‑123 (MQ=255)                       aCGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGGTGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGa                                                                                   <  2:767320/123‑1 (MQ=255)                       aCGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGGTGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTGCTACTACAACAg                                                                   >  2:234222/1‑139 (MQ=255)                            tCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGGTGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTGCTACTACAACAGCaaaa                                                              >  1:738606/1‑139 (MQ=255)                               cTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGGTGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTGCTACTACAACAGCAAAAACt                                                           <  1:448024/139‑1 (MQ=255)                                            tCACCCAATCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGGTGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTGCTACTACAACAGCAAAAACTGGAGCTACTGCtt                                              <  2:529861/139‑1 (MQ=255)                                                 cAATCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGGTGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTGCTACTACAACAGCAAAAACTGGAGCTACTGCTTTGTgg                                         <  2:488871/139‑1 (MQ=255)                                                       aCCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGGTGGAGTTCTCGCCGGTGCACTTCca                                                                                                    <  2:615522/74‑1 (MQ=255)                                                       aCCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGGTGGAGTTCTCGCCGGTGCACTTCca                                                                                                    >  1:615522/1‑74 (MQ=255)                                                               gcgcCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGGTGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTGCTACTACAACAGCAAAAACTGGAGCTACTGCTTTGTGGACTACGAGGAGGGa                           <  1:792626/139‑1 (MQ=255)                                                               gcgcCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGGTGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTGCTACTACAACAGCAAAAACTGGAGCTACTGCTTTGTGGACTACGAGGAGGGa                           <  1:872009/139‑1 (MQ=255)                                                                  ccgcTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGGTg                                                                                                                           >  2:367674/1‑40 (MQ=255)                                                                  ccgcTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGGTg                                                                                                                           <  1:367674/40‑1 (MQ=255)                                                                             aCTTCGCCTTCCGGGCAGAAACGCGGGTGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCgggg                                                                                       <  1:760892/65‑1 (MQ=255)                                                                             aCTTCGCCTTCCGGGCAGAAACGCGGGTGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCgggg                                                                                       >  2:760892/1‑65 (MQ=255)                                                                                        cGGGCAGAAACGCGGGTGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTGCTACTACAACAGCAAAAACTGGAGCTACTGCTTTGTGGACTACGAGGAGGGACAGGGTAGAACCATCAAAGTTATcc  >  2:625570/1‑139 (MQ=255)                                                                                                       |                                                                                                                            GGCTTCTTACGGCTCTATGGCAACGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGATGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTGCTACTACAACAGCAAAAACTGGAGCTACTGCTTTGTGGACTACGAGGAGGGACAGGGTAGAACCATCAAAGTTATCC  >  NC_000913/287875‑288100

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑