Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,032,032	G→A	S222S (TCG→TCA)	uacT →	uric acid permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,032,032	0	G	A	100.0%	108.1 / NA	34	S222S (TCG→TCA)	uacT	uric acid permease
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (16/18);  total (16/18)

AATATGGTAATCCCGTTTATTTAGGTATCTCCTTTGCCGTCTTAATTTTTATCTTGCTCATTACTCGCTATGCGAAAGGATTTATGTCCAACGTCGCCGTATTACTGGGGATTGTATTTGGCTTTTTACTTTCGTGGATGATGAATGAAGTCAATTTATCCGGGCTACATGATGCTTCATGGTTTGCGATTGTTACGCCGATGTCGTTTGGTATGCCGATTTTCGATCCCGTTTCCATTCTGACCATGACTGCCGTGTTAATCAT  >  NC_000913/3031899‑3032163                                                                                                                                      |                                                                                                                                    aaTATGGTAATCCCGTTTATTTAGGTATCGCCTTTGCCGTCTTAATTTTTATCTTGCTCATTACTCGCTATGCGAAAGGATTTATGTCCAACGTCGCCGTATTACTGGGGATTGTATTTGGCTTTTTACTTTCATGgat                                                                                                                                <  1:259747/139‑1 (MQ=255)        tAATCCCGTTTATTTAGGTATCTCCTTTGCCGTCTTAATTTTTATCTTGCTCATTACTCGCTATGCGAAAGGATTTATGTCCAACGTCGCCGTATTACTGGGGATTGTATTTGGCTTTTTACTTTCATGGATGatgaat                                                                                                                         >  2:1006478/1‑139 (MQ=255)                      taGGTATCTCCTTTGCCGTCTTAATTTTTATCTTGCTCATTACTCGCTATGCGAAAGGATTTATGTCCAACGTCGCCGTATTACTGGGGATTGTATTTGGCTTTTTACTTTCATGGATGATGAATGAAGTCAATTTATc                                                                                                           <  2:583815/139‑1 (MQ=255)                              tcCTTTGCCGTCTTAATTTTTATCTTGCTCATTACTCGCTATGCGAAAGGATTTATGTCCAACGTCGCCGTATTACTGGGGATTGTATTTGGCTTTTTACTTTCATGGATGATGAATGAAGTCAATTTATCCggg                                                                                                       >  2:472909/1‑135 (MQ=255)                              tcCTTTGCCGTCTTAATTTTTATCTTGCTCATTACTCGCTATGCGAAAGGATTTATGTCCAACGTCGCCGTATTACTGGGGATTGTATTTGGCTTTTTACTTTCATGGATGATGAATGAAGTCAATTTATCCggg                                                                                                       <  1:472909/135‑1 (MQ=255)                                 tttGCCGTCTTAATTTTTATCTTGCTCATTACTCGCTATGCGAAAGGATTTATGTCCAACGTCGCCGTATTACTGGGGATTGTATTTGGCTTTTTACTTTCATGGATGATGAATGAAGTCAATTTATCCGGGCTACatg                                                                                                <  1:274690/139‑1 (MQ=255)                                        tCTTAATTTTTATCTTGCTCATTACTCGCTATGCGAAAGGATTTATGTCCAACGTCGCCGTATTACTGGGGATTGTATTTGGCTTTTTACTTTCATGGATGATGAATGAAGTCAATTTATCCGGGCTACATGATGCTTc                                                                                         >  2:132111/1‑139 (MQ=255)                                          ttAATTTTTATCTTGCTCATTACTCGCTATGCGAAAGGATTTATGTCCAACGTCGCCGTATTACTGGGGATTGTATTTGGCTTTTTACTTTCATGGATGATGAATGAAGTCAATTTATCCGGGCTACATGATGCTTCAt                                                                                       >  2:182453/1‑139 (MQ=255)                                                            aTTACTCGCTATGCGAAAGGATTTATGTCCAACGTCGCCGTATTACTGGGGATTGTATTTGGCTTTTTACTTTCATGGATGATGAATGAAGTCAATTTATCCGGGCTACATGATGCTTCATGGTTTGCGATTGTTACGc                                                                     <  1:1006478/139‑1 (MQ=255)                                                              tACTCGCTATGCGAAAGGATTTATGTCCAACGTCGCCGTATTACTGGGGATTGTATTTGGCTTTTTACTTTCATGGATGATGAATGAAGTCAATTTATCCGGGCTACATGATGCTTCATGGTTTGCGATTGTTACGCCg                                                                   <  2:397825/139‑1 (MQ=255)                                                                     tATGCGAAAGGATTTATGTCCAACGTCGCCGTATTACTGGGGATTGTATTTGGCTTTTTACTTTCATGGATGATGAATGAAGTCAATTTATCCGGGCTACATGATGCTTCATGGTTTGCGATTGTTACGCCGATGTCGt                                                            >  2:1002099/1‑139 (MQ=255)                                                                       tGCGAAAGGATTTATGTCCAACGTCGCCGTATTACTGGGGATTGTATTTGGCTTTTTACTTTCATGGATGATGAATGAAGTCAATTTATCCGGGCTACATGATGCTTCATGGTTTGCGATTGTTACGCCGATGTCGttt                                                          <  1:132111/139‑1 (MQ=255)                                                                           aaaGGATTTATGTCCAACGTCGCCGTATTACTGGGGATTGTATTTGGCTTTTTACTTTCATGGATGATGAATGAAGTCAATTTATCCgg                                                                                                        >  1:870447/1‑89 (MQ=255)                                                                           aaaGGATTTATGTCCAACGTCGCCGTATTACTGGGGATTGTATTTGGCTTTTTACTATCATGGATGATGAATGAAGTCAATTTATCCgg                                                                                                        <  2:870447/89‑1 (MQ=255)                                                                                   tATGTCCAACGTCGCCGTATTACTGGGGATTGTATTTGGCTTTTTACTTTCATGGATGATGAATGAAGTCAATTTATCCGGGCTACATGATGCTTCATGGTTTGCGATTGTTACGCCg                                                                   >  1:574072/1‑118 (MQ=255)                                                                                   tATGTCCAACGTCGCCGTATTACTGGGGATTGTATTTGGCTTTTTACTTTCATGGATGATGAATGAAGTCAATTTATCCGGGCTACATGATGCTTCATGGTTTGCGATTGTTACGCCg                                                                   <  2:574072/118‑1 (MQ=255)                                                                                           aCGTCGCCGTATTACTGGGGATTGTATTTGGCTTTTTACTTTCATGGATGATGAATGAAGTCAATTTATCCGGGCTACATGATGCTTCATGGTTTGCGATTGTTACGCCGATGTCGTTTGGTATGCCGATTTTCGATcc                                      <  1:194425/139‑1 (MQ=255)                                                                                               cGCCGTATTACTGGGGATTGTATTTGGCTTTTTACTTTCATGGATGATGAATGAAGTCAATTTATCCGGGCTACATGATGCTTCATGGTTTGCGATTGTTACGCCGATGTCGTTTGGTATGCCGATTTTCGATc                                       <  2:844439/134‑1 (MQ=255)                                                                                               cGCCGTATTACTGGGGATTGTATTTGGCTTTTTACTTTCATGGATGATGAATGAAGTCAATTTATCCGGGCTACATGATGCTTCATGGTTTGCGATTGTTACGCCGATGTCGTTTGGTATGCCGATTTTCGATc                                       >  1:844439/1‑134 (MQ=255)                                                                                                gCCGTATTACTGGGGATTGTATTTGGCTTTTTACTTTCATGGATGATGAATGAAGTCAATTTATCCGGGCTACATGATGCTTCATGGTTTGCGATTGTTACGCCGATGTCGTTTGGTATGCCGATTTTCGATCCCGttt                                 <  1:182453/139‑1 (MQ=255)                                                                                                      ttACTGGGGATTGTATTTGGCTTTTTACTTTCATGGATGATGAATGAAGTCAATTTATCCGGGCTACATGATGCTTCATGGTTTGCGATTGTTACGCCGATGTCGTTTGGTATGCCGATTTTCGATCCCGTTTCCATTc                           >  2:263359/1‑139 (MQ=255)                                                                                                               aTTGTATTTGGCTTTTTACTTTCATGGATGATGAATGAAGTCAATTTATCCGGGCTACATGATGCTTCATGGTTTGCGATTGTTACGCCGATGTCGTTTGGTATGCCGATTTTCGATCCCGTTTCCATTCTGACCATGa                  <  1:1002099/139‑1 (MQ=255)                                                                                                                 tGTATTTGGCTTTTTACTTTCATGGATGATGAATGAAGTCAATTTATCCGGGCTACATGATGCTTCATGGTTTGCGATTGTTACGCCGATGTCGTTTGGTATGCCGATTTTCGATCCCGTTTCCATTCTGACCATGACt                >  2:335945/1‑139 (MQ=255)                                                                                                                 tGTATTTGGCTTTTTACTTTCATGGATGATGAATGAAGTCAATTTATCCGGGCTACATGATGCTTCATGGTTTGCGATTGTTACGCCGATGTCGTTTGGTATGCCGATTTTCGATCCCGTTTCCATTCTGACCATGACt                >  1:18727/1‑139 (MQ=255)                                                                                                                  gTATTTGGCTTTTTACTTTCATGGATGATGAATGAAGTCAATTTATCCGGGCTACATGATGCTTCATGGTTTGCGATTGTTACGCCGATGTCGTTTGGTATGCCGATTTTCGATCCCGTTTCCATTCTGACCATGACTg               <  2:18727/139‑1 (MQ=255)                                                                                                                  gTATTTGGCTTTTTACTTTCATGGATGATGAATGAAGTCAATTTATCCGGGCTACATGATGCTTCATGGTTTGCGATTGTTACGCCGATGTCGTTTGGTATGCCGATTTTCGATCCCGTTTCCATTCTGACCATGACTg               <  2:827626/139‑1 (MQ=255)                                                                                                                      ttGGCTTTTTACTTTCATGGATGATGAATGAAGTCAATTTATCCGGGCTACATGATGCTTCATGGTTTGCGATTGTTACGCCGATGTCGTTTGGTATGCCGATTTTCGATCCCGTTTCCATTCTGACCATGACTGCCgt           >  1:843130/1‑139 (MQ=255)                                                                                                                        ggCTTTTTACTTTCATGGATGATGAATGAAGTCAATTTATCCGGGCTACATGATGCTTCATGGTTTGCGATTGTTACGCCGATGTCGTTTGGTATGCCGATTTTCGATCCCGTTTCCAtt                            <  1:276709/120‑1 (MQ=255)                                                                                                                        ggCTTTTTACTTTCATGGATGATGAATGAAGTCAATTTATCCGGGCTACATGATGCTTCATGGTTTGCGATTGTTACGCCGATGTCGTTTGGTATGCCGATTTTCGATCCCGTTTCCAtt                            >  2:276709/1‑120 (MQ=255)                                                                                                                        ggCTTTTTACTTTCATGGATGATGAATGAAGTCAATTTATCCGGGCTACATGATGCTTCATGGTTTGCGATTGTTACGCCGATGTCGTTTGGTATGCCGATTTTCGATCCCGTTTCCATTCTGACCATGACTGCCgtgt         >  2:34649/1‑139 (MQ=255)                                                                                                                             tttACTTTCATGGATGATGAATGAAGTCAATTTATCCGGGCTACat                                                                                                 <  2:735002/46‑1 (MQ=255)                                                                                                                             tttACTTTCATGGATGATGAATGAAGTCAATTTATCCGGGCTACat                                                                                                 >  1:735002/1‑46 (MQ=255)                                                                                                                              ttACTTTCATGGATGATGAATGAAGTCAATTTATCCGGGCTACATGATGCTTCATGGTTTGCGATTGTTACGCCGATGTCGTTTGGTATGCCGATTTTCGATCCCGTTTCCATTCTGACCATGACTGCCGTGTTAatca   <  1:263359/139‑1 (MQ=255)                                                                                                                               tACTTTCATGGATGATGAATGAAGTCAATTTATCCGGGCTACATGATGCTTCATGGTTTGCGATTGTTACGCCGATGTCGTTTGGTATGCCGATTTTCGATCCCGTTTCCATTCTGACCATGACTGCCGTGTTAatcat  >  2:546368/1‑139 (MQ=255)                                                                                                                                      |                                                                                                                                    AATATGGTAATCCCGTTTATTTAGGTATCTCCTTTGCCGTCTTAATTTTTATCTTGCTCATTACTCGCTATGCGAAAGGATTTATGTCCAACGTCGCCGTATTACTGGGGATTGTATTTGGCTTTTTACTTTCGTGGATGATGAATGAAGTCAATTTATCCGGGCTACATGATGCTTCATGGTTTGCGATTGTTACGCCGATGTCGTTTGGTATGCCGATTTTCGATCCCGTTTCCATTCTGACCATGACTGCCGTGTTAATCAT  >  NC_000913/3031899‑3032163

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑