Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,085,461 A→G S151P (TCC→CCC)  speA ← biosynthetic arginine decarboxylase, PLP‑binding

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,085,4610AG100.0% 104.0 / NA 30S151P (TCC→CCC) speAbiosynthetic arginine decarboxylase, PLP‑binding
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (14/16);  total (14/16)

ATAGACCTTGTGCCCCATCTTCTCGCCAATTAATGCCAGGCGGATATATTCGCGGTCTTTATAACCGTTGCAGACGATGACGCTACGGGTCATGCCAGCATGTGCCAGTACTGCCATCAACTCGGCTTTGGAACCGGCTTCCAGACCCAGCGGTTCGCCCGAATGAATCAGGGACTCAATCACGCGGCGGTGCTGGTTAACTTTGATCGGATAAACAAGGAAGTAATCGCCGTTATAGCCGTAGGATTCCCTCGCACGTTTGAACGC  >  NC_000913/3085330‑3085596
                                                                                                                                   |                                                                                                                                       
aTAGACCTTGTGCCCCATCTTCTCGCCAATTAATGCCAGGCGGATATATTCGCGGTCTTTATAACCGTTGCAGACGATGACGCTACGGGTCATGCCAGCATGTGCCAGTACTGCCATCAACTCGGCTTTGGGACCGGCt                                                                                                                                  >  1:242789/1‑139 (MQ=255)
          tgCCCCATCTTCTCGCCAATTAATGCCAGGCGGATATATTCGCGGTCTTTATAACCGTTGCAGACGATGACGCTACGGGTCATGCCAGCATGTGCCAGTACTGCCATCAACTCGGCTTTGGGACCGGCTTCCAGACCCa                                                                                                                        >  2:575347/1‑139 (MQ=255)
                         ccAATTAATGCCAGGCGGATATATTCGCGGTCTTTATAACCGTTGCAGACGATGACGCTACGGGTCATGCCAGCATGTGCCAGTACTGCCATCAACTCGGCTTTGGGACCGGCTTCCAGACCCAGCGGTTCGCCCgaat                                                                                                         <  1:575347/139‑1 (MQ=255)
                                  gCCAGGCGGATATATTCGCGGTCTTTATAACCGTTGCAGACGATGACGCTACGGGTCATGCCAGCATGTGCCAGTACTGCCATCAACTCGGCTTTGGGACCGGCTTCCAGACCCAGCGGTTCGCCCGAATGAATCAggg                                                                                                <  2:242789/139‑1 (MQ=255)
                                           atatatTCGCGGTCTTTATAACCGTTGCAGACGATGACGCTACGGGTCATGCCAGCATGTGCCAGTACTGCCATCAACTCGGCTTTGGGACCGGCTTCCAGACCCAGCGGTTCGCCCGAATGAATCAGGGACTCAATCa                                                                                       <  1:835304/139‑1 (MQ=255)
                                                ttCGCGGTCTTTATAACCGTTGCAGACGATGACGCTACGGGTCATGCCAGCATGTGCCAGTACTGCCATCAACTCGGCTTTGGGACCGGCTTCCAGACCCAGCGGTTCGCCCGAATGAATc                                                                                                    <  1:504384/121‑1 (MQ=255)
                                                ttCGCGGTCTTTATAACCGTTGCAGACGATGACGCTACGGGTCATGCCAGCATGTGCCAGTACTGCCATCAACTCGGCTTTGGGACCGGCTTCCAGACCCAGCGGTTCGCCCGAATGAATc                                                                                                    >  2:504384/1‑121 (MQ=255)
                                                   gcgGTCTTTATAACCGTTGCAGACGATGACGCTACGGGTCATGCCAGCATGTGCCAGTACTGCCATCAACTCGGCTTTGGGACCGGCTTCCAGACCCAGCGGTTCGCCCGAATGAATCAGGGACTCAATCACGcggcgg                                                                               <  1:375845/139‑1 (MQ=255)
                                                     ggTCTTTATAACCGTTGCAGACGATGACGCTACGGGTCATGCCAGCATGTGCCAGTACTGCCATCAACTCGGCTTTGGGACCGGCTTCCAGACCCAGCGGTTCGCCCGAATGAATCAGGGACTCAATCACGCGGCGGTg                                                                             >  1:779708/1‑139 (MQ=255)
                                                                 cGTTGCAGACGATGACGCTACGGGTCATGCCAGCATGTGCCAGTACTGCCATCAACTCGGCTTTGGGACCGGCTTCCAGACCCAGCGGTTCGCCCGAATGAATCAGGGACTCAATCACGCGGCGGTGCTGGTTAACttt                                                                 >  2:650042/1‑139 (MQ=255)
                                                                   ttGCAGACGATGACGCTACGGGTCATGCCAGCATGTGCCAGTACTGCCATCAACTCGGCTTTGGGACCGGCTTCCAGACCCAGCGGTTCGCCCGAATGAATCAGGGACTCAATCACGCGGCGGTGCTGGTTAACTTTGa                                                               >  2:518192/1‑139 (MQ=255)
                                                                    tGCAGACGATGACGCTACGGGTCATGCCAGCATGTGCCAGTACTGCCATCAACTCGGCTTTGGGACCGGCTTCCAGACCCAGCGGTTCGCCCGAATGAATCAGGGACTCAATCACGCGGCGGTGCTGGTTAACTTTGAt                                                              <  1:650042/139‑1 (MQ=255)
                                                                      cAGACGATGACGCTACGGGTCATGCCAGCATGTGCCAGTACTGCCATCAACTCGGCTTTGGGACCGGCTTCCAGACCCAGCGGTTCGCCCGAATGAATCAGGGACTCAATCACGCGGCGGTGCTGGTTAACTTTGATCg                                                            <  2:503155/139‑1 (MQ=255)
                                                                         aCGATGACGCTACGGGTCATGCCAGCATGTGCCAGTACTGCCATCAACTCGGCTTTGGGACCGGCTTCCAGACCCAGCGGTTCGCCCGAATGAATCAGGGACTCAATCACGCGGCGGTGCTGGTTAACTTTGATCGGAt                                                         <  1:518192/139‑1 (MQ=255)
                                                                          cGATGACGCTACGGGTCATGCCAGCATGTGCCAGTACTGCCATCAACTCGGCTTTGGGACCGGCTTCCAGACCCAGCGGTTCGCCCGAATGAATCAGGGACTCAATCACGCGGCGGTGCTGGTTAACTTTGATCGGATa                                                        <  2:779708/139‑1 (MQ=255)
                                                                              gACGCTACGGGTCATGCCAGCATGTGCCAGTACTGCCATCAACTCGGCTTTGGGACCGGCTTCCAGACCCAGCGGTTCGCCCGAATGAATCAGGGACTCAATCACGCGGCGGTGCTGGTTAACTTTGATCGGATAAACa                                                    <  1:718184/139‑1 (MQ=255)
                                                                                     cGGGTCATGCCAGCATGTGCCAGTACTGCCATCAACTCGGCTTTGGGACCGGCTTCCAGACCCAGCGGTTCGCCCGAATGAATCAg                                                                                                  >  1:293829/1‑86 (MQ=255)
                                                                                     cGGGTCATGCCAGCATGTGCCAGTACTGCCATCAACTCGGCTTTGGGACCGGCTTCCAGACCCAGCGGTTCGCCCGAATGAATCAg                                                                                                  <  2:293829/86‑1 (MQ=255)
                                                                                                  cATGTGCCAGTACTGCCATCAACTCGGCTTTGGGACCGGCTTCCAGACCCAGCGGTTCGCCCGAATGAATCAGGGACTCAATCACGCGGCGGTGCTGGTTAACTTTGATCGGATAAACAAGGAAGTAATCGCCGTtata                                >  2:783932/1‑139 (MQ=255)
                                                                                                   aTGTGCCAGTACTGCCATCAACTCGGCTTTGGGACCGGCTTCCAGACCCAGCGGt                                                                                                                   >  2:415974/1‑55 (MQ=255)
                                                                                                   aTGTGCCAGTACTGCCATCAACTCGGCTTTGGGACCGGCTTCCAGACCCAGCGGt                                                                                                                   <  1:415974/55‑1 (MQ=255)
                                                                                                     gtgCCAGTACTGCCATCAACTCGGCTTTGGGACCGGCTTCCAGACCCAGCGGTTCGCCCGAATGAATCAGGGACTCAATCACGCGGCGGTGCTGGTTAACTTTGATCGGATAAACAAGGAAGTAATCGCCGTTATAGcc                             <  2:448102/139‑1 (MQ=255)
                                                                                                                 ccATCAACTCGGCTTTGGGACCGGCTTCCAGACCCAGCGGTTCGCCCGAATGAATCAGGGACTCAATCACGCGGCGGTGCTGGTTAACTTTGATCGGATAAACAAGGAAGTAATCGCCGTTATAGCTGTAGGATTCCCt                 >  2:759641/1‑139 (MQ=255)
                                                                                                                 ccATCAACTCGGCTTTGGGACCGGCTTCCAGACCCAGCGGTTCGCCCGAATGAATCAGGGACTCAATCACGCGGCGGTGCTGGTTAACTTTGATCGGATAAACAAGGAAGTAATCGCCGTTATAGCCGTAGGATTCCCt                 >  2:823965/1‑139 (MQ=255)
                                                                                                                 ccATCAACTCGGCTTTGGGACCGGCTTCCAGACCCAGCGGTTCGCCCGAATGAATCAGGGACTCAATCACGCGGCGGTGCTGGTTAACTTTGATCGGATAAACAAGGAAGTAATCGCCGTTATAGCCGTAGGATTCCCt                 >  1:16835/1‑139 (MQ=255)
                                                                                                                   aTCAACTCGGCTTTGGGACCGGCTTCCAGACCCAGCGGTTCGc                                                                                                               >  2:320444/1‑43 (MQ=255)
                                                                                                                   aTCAACTCGGCTTTGGGACCGGCTTCCAGACCCAGCGGTTCGc                                                                                                               <  1:320444/43‑1 (MQ=255)
                                                                                                                        cTCGGCTTTGGGACCGGCTTCCAGACCCAGCGGTTCGCCCGAATGAATCAGGGACt                                                                                             >  2:546196/1‑56 (MQ=255)
                                                                                                                        cTCGGCTTTGGGACCGGCTTCCAGACCCAGCGGTTCGCCCGAATGAATCAGGGACt                                                                                             <  1:546196/56‑1 (MQ=255)
                                                                                                                                tGGGACCGGCTTCCAGACCCAGCGGTTCGCCCGAATGAATCAGGGACTCAATCACGCGGCGGTGCTGGTTAACTTTGATCGGATAAACAAGGAAGTAATCGCCGTTATAGCCGTAGGATTCCCTCGCACGTTTGAAcgc  <  1:667852/139‑1 (MQ=255)
                                                                                                                                   |                                                                                                                                       
ATAGACCTTGTGCCCCATCTTCTCGCCAATTAATGCCAGGCGGATATATTCGCGGTCTTTATAACCGTTGCAGACGATGACGCTACGGGTCATGCCAGCATGTGCCAGTACTGCCATCAACTCGGCTTTGGAACCGGCTTCCAGACCCAGCGGTTCGCCCGAATGAATCAGGGACTCAATCACGCGGCGGTGCTGGTTAACTTTGATCGGATAAACAAGGAAGTAATCGCCGTTATAGCCGTAGGATTCCCTCGCACGTTTGAACGC  >  NC_000913/3085330‑3085596

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: