Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,104,065	G→A	*351* (TAG→TAA)	mutY →	adenine DNA glycosylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,104,065	0	G	A	100.0%	144.1 / NA	45	*351* (TAG→TAA)	mutY	adenine DNA glycosylase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (23/22);  total (23/22)

TTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAGGCAATGCGCTCTGGTATAACTTAGCGCAACCGCCGTCAGTTGGCCTAGCGGCTCCCGTGGAGCGTTTGTTACAGCAGTTACGCACTGGCGCGCCGGTTTAGCGCGTGAGTCGATAAAGAGGATGATTTATGAGCAGAACGATTTTTTGTACTTTCCTGCAACGTGAAGCAGAAGGTCAGGATTTTCAGCTGTACCCCGGCGAGCTGGGAAAACGCATCTATAACGAGATCTCCAAA  >  NC_000913/3103929‑3104200                                                                                                                                         |                                                                                                                                        ttCCCGTGTCGTCATTCACCGGCTGCATGGATGAAGGCAATGCGCTCTGGTATAACTTAGCGCAACCGCCGTCAGTTGGCCTAGCGGCTCCCGTGGAGCGTTTGTTACAGCAGTTACGCACTGGCGCGCCGGTTTAacg                                                                                                                                       >  2:218355/1‑139 (MQ=255)   cccGTGTCGTCATTCACCGGCTGCATGGATGAAGGCAATGCGCTCTGGTATAACTTAGCGCAACCGCCGTCAGTTGGCCTAGCGGCTCCCGTGGAGCGTTTGTTACAGCAGTTACGCACTGGCGCGCCGGTTTAAcgcg                                                                                                                                     <  1:218355/139‑1 (MQ=255)           gtcATTCACCGGCTGCATGGATGAAGGCAATGCGCTCTGGTATAACTTAGCGCAACCGCCGTCAGTTGGCCTAGCGGCTCCCGTGGAGCGTTTGTTACAGCAGTTACGCACTGGCGCGCCGGTTTAACGCGTGAGTCGa                                                                                                                             >  2:993487/1‑139 (MQ=255)             cATTCACCGGCTGCATGGATGAAGGCAATGCGCTCTGGTATAACTTAGCGCAACCGCCGTCAGTTGGCCTAGCGGCTCCCGTGGAGCGTTTGTTACAGCAGTTACGCACTGGCGCGCCGGTTTAACGCGTGAGTCGATa                                                                                                                           <  2:943735/139‑1 (MQ=255)              aTTCACCGGCTGCATGGATGAAGGCAATGCGCTCTGGTATAACTTAGCGCAACCGCCGTCAGTTGGCCTAGCGGCTCCCGTGGAGCGTTTGTTACAGCAGTTACGCACTGGCGCGCCGGTTTAACGCGTGAGt                                                                                                                                <  1:89265/133‑1 (MQ=255)              aTTCACCGGCTGCATGGATGAAGGCAATGCGCTCTGGTATAACTTAGCGCAACCGCCGTCAGTTGGCCTAGCGGCTCCCGTGGAGCGTTTGTTACAGCAGTTACGCACTGGCGCGCCGGTTTAACGCGTGAGt                                                                                                                                >  2:89265/1‑133 (MQ=255)                      gCTGCATGGATGAAGGCAATGCGCTCTGGTATAACTTAGCGCAACCGCCGTCAGTTGGCCTAGCGGCTCCCGTGGAGCGTTTGTTACAGCAGTTACGCACTGGCGCGCCGGTTTAACGCGTGAGTCGATAAAGAGgatg                                                                                                                  >  2:565967/1‑139 (MQ=255)                      gCTGCATGGATGAAGGCAATGCGCTCTGGTATAACTTAGCGCAACCGCCGTCAGTTGGCCTAGCGGCTCCCGTGGAGCGTTTGTTACAGCAGTTACGCACTGGCGCGCCGGTTTAACGCGTGAGTCGATAAAGAGgatg                                                                                                                  >  1:743606/1‑139 (MQ=255)                                         tGCGCTCTGGTATAACTTAGCGCAACCGCCGTCAGTTGGCCTAGCGGCTCCCGTGGAGCGTTTGTTACAGCAGTTACGCACTGGCGCGCCGGTTTAACGCGTGAGTCGATAAAGAGGATGATTTATGAGCAGAACGAtt                                                                                               >  1:439509/1‑139 (MQ=255)                                                tGGTATAACTTAGCGCAACCGCCGTCAGTTGGCCTAGCGGCTCCCGTGGAGCGTTTGTTACAGCAGTTACGCACTGGCGCGCCGGTTTAACGCGTGAGTCGATAAAGAGGATGATTTATGAGCAGAACGATTTTTTGTa                                                                                        <  2:26789/139‑1 (MQ=255)                                                tGGTATAACTTAGCGCAACCGCCGTCAGTTGGCCTAGCGGCTCCCGTGGAGCGTTTGTTACAGCAGTTACGCACTGGCGCGCCGGTTTAACGCGTGAGTCGATAAAGAGGATGATTTATGAGCAGAACGATTTTTTGTa                                                                                        <  1:87425/139‑1 (MQ=255)                                                          tAGCGCAACCGCCGTCAGTTGGCCTAGCGGCTCCCGTGGAGCGTTTGTTACAGCAGTTACGCACTGGCGCGCCGGTTTAACGCGTGAGTCGATAAAGAGGATGATTTATGAGCAGAACGATTTTTTGTACTTTCCTGCa                                                                              <  1:993487/139‑1 (MQ=255)                                                            gcgcAACCGCCGTCAGTTGGCCTAGCGGCTCCCGTGGAGCGTTTGTTACAGCAGTTACGCACTGGCGCGCCGGTTTAACGCGTGAGTCGATAAAGAGGATGATTTATGAGCAGAACGATTTTTTGTACTTTCCTGCAAc                                                                            >  2:669796/1‑139 (MQ=255)                                                            gcgcAACCGCCGTCAGTTGGCCTAGCGGCTCCCGTGGAGCGTTTGTTACAGCAGTTACGCACTGGCGCGCCGGTTTAACGCGTGAGTCGATAAAGAGGATGATTTATGAGCAGAACGATTTTTTGTACTTTCCTGCAAc                                                                            >  1:194274/1‑139 (MQ=255)                                                             cgcAACCGCCGTCAGTTGGCCTAGCGGCTCCCGTGGAGCGTTTGTTACAGCAGTTACGCACTGGCGCGCCGGTTTAACGCGTGAGTCGATAAAGAGGATGATTTATGAGCAGAACGATTTTTTGTACTTTCCTGCAACg                                                                           >  1:775313/1‑139 (MQ=255)                                                                  ccgccgTCAGTTGGCCTAGCGGCTCCCGTGGAGCGTTTGTTACAGCAGTTACGCACTGGCGCGCCGGTTTAACGCGTGAGTCGATaaa                                                                                                                         >  2:217336/1‑88 (MQ=255)                                                                  ccgccgTCAGTTGGCCTAGCGGCTCCCGTGGAGCGTTTGTTACAGCAGTTACGCACTGGCGCGCCGGTTTAACGCGTGAGTCGATaaa                                                                                                                         <  1:217336/88‑1 (MQ=255)                                                                                         tCCCGTGGAGCGTTTGTTACAGCAGTTACGCACTGGCGCGCCGGTTTAACGCGTGAGt                                                                                                                                <  2:931522/58‑1 (MQ=255)                                                                                         tCCCGTGGAGCGTTTGTTACAGCAGTTACGCACTGGCGCGCCGGTTTAACGCGTGAGt                                                                                                                                >  1:931522/1‑58 (MQ=255)                                                                                              tGGAGCGTTTGTTACAGCAGTTACGCACTGGCGCGCCGGTTTAACGCGTg                                                                                                                                   <  1:164181/50‑1 (MQ=255)                                                                                              tGGAGCGTTTGTTACAGCAGTTACGCACTGGCGCGCCGGTTTAACGCGTg                                                                                                                                   >  2:164181/1‑50 (MQ=255)                                                                                              tGGAGCGTTTGTTACAGCAGTTACGCACTGGCGCGCCGGTTTAACGCGTGAGTCGATAAag                                                                                                                        <  1:417929/61‑1 (MQ=255)                                                                                              tGGAGCGTTTGTTACAGCAGTTACGCACTGGCGCGCCGGTTTAACGCGTGAGTCGATAAag                                                                                                                        >  2:417929/1‑61 (MQ=255)                                                                                                     tttGTTACAGCAGTTACGCACTGGCGCGCCGGTTTAACGCGTGAGTCGATAAAGAGGATGATTTATGAGCAGAACGATTTTTTGTACTTTCCTGCAACGTGAAGCAGAAGGTCAGGATTTTCAGCTGTACCCCGGc                                      >  1:478657/1‑136 (MQ=255)                                                                                                     tttGTTACAGCAGTTACGCACTGGCGCGCCGGTTTAACGCGTGAGTCGATAAAGAGGATGATTTATGAGCAGAACGATTTTTTGTACTTTCCTGCAACGTGAAGCAGAAGGTCAGGATTTTCAGCTGTACCCCGGc                                      <  2:478657/136‑1 (MQ=255)                                                                                                                 gTTACGCACTGGCGCGCCGGTTTAACGCGTGAGTCGATAAAGAGGATTATTTATGAGCATAACGATTTTTTGTACTTTCCTGCAACGTGAAGCAGAAGGTCAGGAtttt                                                     <  1:653090/109‑1 (MQ=255)                                                                                                                 gTTACGCACTGGCGCGCCGGTTTAACGCGTGAGTCGATAAAGAGGATGATTTATGAGCAGAACGATTTTTTGTACTTTCCTGCAACGTGAAGCAGAAGGTCAGGAtttt                                                     >  2:653090/1‑109 (MQ=255)                                                                                                                  ttACGCACTGGCGCGCCGGTTTAACGCGTGAGTCGATAAAGAGgat                                                                                                                   <  1:729477/46‑1 (MQ=255)                                                                                                                  ttACGCACTGGCGCGCCGGTTTAACGCGTGAGTCGATAAAGAGgat                                                                                                                   >  2:729477/1‑46 (MQ=255)                                                                                                                  ttACGCACTGGCGCGCCGGTTTAACGCGTGAGTCGATAAAGAGGATGATTTATGAGCAGAACGATTTTTTGTACTTTCCTg                                                                                <  1:652554/81‑1 (MQ=255)                                                                                                                  ttACGCACTGGCGCGCCGGTTTAACGCGTGAGTCGATAAAGAGGATGATTTATGAGCAGAACGATTTTTTGTACTTTCCTg                                                                                >  2:652554/1‑81 (MQ=255)                                                                                                                    aCGCACTGGCGCGCCGGTTTAACGCGTGAGTCGATAAAGAGGATGATTTATGAGCAGAACg                                                                                                  <  2:237286/61‑1 (MQ=255)                                                                                                                    aCGCACTGGCGCGCCGGTTTAACGCGTGAGTCGATAAAGAGGATGATTTATGAGCAGAACg                                                                                                  >  1:237286/1‑61 (MQ=255)                                                                                                                    aCGCACTGGCGCGCCGGTTTAACGCGTGAGTCGATAAAGAGGATGATTTATGAGCAGAACGATTTTTTGTACtt                                                                                     >  1:541033/1‑74 (MQ=255)                                                                                                                    aCGCACTGGCGCGCCGGTTTAACGCGTGAGTCGATAAAGAGGATGATTTATGAGCAGAACGATTTTTTGTACtt                                                                                     <  2:541033/74‑1 (MQ=255)                                                                                                                    aCGCACTGGCGCGCCGGTTTAACGCGTGAGTCGATAAAGAGGATGATTTATGAGCAGAACGATTTTTTGTACTTTCCTGCAACGTGAAGCAGAAGGTCAGGATTTTCAGCTGTACCCCGGCGAGCTGGGAAAACGCATc                    >  2:878517/1‑139 (MQ=255)                                                                                                                          tGGCGCGCCGGTTTAACGCGTGAGTCGATAAAGAGGATGATTTATGAGCAGAACGATTTTTTGTACTTTCCTGCAACGTGAAGCAGAAGGTCAGGATTTTCAGCTGTACCCCGGCGAGCTGGGaaaa                          >  1:137234/1‑127 (MQ=255)                                                                                                                          tGGCGCGCCGGTTTAACGCGTGAGTCGATAAAGAGGATGATTTATGAGCAGAACGATTTTTTGTACTTTCCTGCAACGTGAAGCAGAAGGTCAGGATTTTCAGCTGTACCCCGGCGAGCTGGGaaaa                          <  2:137234/127‑1 (MQ=255)                                                                                                                             cgcgcCGGTTTAACGCGTGAGTCGATAAAGAGGATGATTTATGAGCAGAACGATTTTTTGTACTTTCCTGCAACGTGAAGCAGAAGGTCAGGATTTTCAGCTGTACCCCGGCGAGCTGGGAAAACGCATCTATAACgag           <  2:607470/139‑1 (MQ=255)                                                                                                                             cgcgcCGGTTTAACGCGTGAGTCGATAAAGAGGATGATTTATGAGCAGAACGATTTTTTGTACTTTCCTGCAACGTGAAGCAGAAGGTCAGGATTTTCAGCTGTACCCCGGCGAGCTGGGAAAACGCATCTATAACgag           <  1:565967/139‑1 (MQ=255)                                                                                                                             cgcgcCGGTTTAACGCGTGAGTCGATAAAGAGGATGATTTATGAGCAGAACGATTTTTTGTACTTTCCTGCAACGTGAAGCAGAAGGTCAGGATTTTCAGCTGTACCCCGGCGAGCTGGGAAAACGCATCTATAACgag           <  2:439509/139‑1 (MQ=255)                                                                                                                               cgcCGGTTTAACGCGTGAGTCGATAAAGAGGATGATTTATGAGCAGAACGATTTTTTGTACTTTCCTGCAACGTGAAGCAGAAGGTCAGGATTTTCAGCTGTACCCCGGCGAGCTGGGAAAACGCATCTATAACGAGAt         >  1:451280/1‑139 (MQ=255)                                                                                                                                 ccGGTTTAACGCGTGAGTCGATAAAGAGGATGATTTATGAGCAGAACGATTTTTTGTACTTTCCTGCAACGTGAAGCAGAAGGTCAGGATTTTCAGCTGTACCCCGGCGAGCTGGGAAAACGCATCTATAACGAGAtct       >  1:90140/1‑139 (MQ=255)                                                                                                                                      ttAACGCGTGAGTCGATAAAGAGGATGATTTATGAGCAGAACg                                                                                                  >  1:329363/1‑43 (MQ=255)                                                                                                                                      ttAACGCGTGAGTCGATAAAGAGGATGATTTATGAGCAGAACg                                                                                                  <  2:329363/43‑1 (MQ=255)                                                                                                                                      ttAACGCGTGAGTCGATAAAGAGGATGATTTATGAGCAGAACGATTTTTTGTACTTTCCTGCAACGTGAAGCAGAAGGTCAGGATTTTCAGCTGTACCCCGGCGAGCTGGGAAAACGCATCTATAACGAGATCTCCaaa  <  2:194274/139‑1 (MQ=255)                                                                                                                                         |                                                                                                                                        TTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAGGCAATGCGCTCTGGTATAACTTAGCGCAACCGCCGTCAGTTGGCCTAGCGGCTCCCGTGGAGCGTTTGTTACAGCAGTTACGCACTGGCGCGCCGGTTTAGCGCGTGAGTCGATAAAGAGGATGATTTATGAGCAGAACGATTTTTTGTACTTTCCTGCAACGTGAAGCAGAAGGTCAGGATTTTCAGCTGTACCCCGGCGAGCTGGGAAAACGCATCTATAACGAGATCTCCAAA  >  NC_000913/3103929‑3104200

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑