Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,248,939	T→C	intergenic (+117/‑30)	mzrA → / → yqjC	modulator of EnvZ/OmpR regulon/DUF1090 family putative periplasmic protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,248,939	0	T	C	100.0%	191.6 / NA	53	intergenic (+117/‑30)	mzrA/yqjC	modulator of EnvZ/OmpR regulon/DUF1090 family putative periplasmic protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (28/25);  total (28/25)

CTCTCATCGCTTCGGATAACTTCCTGTTATCCGAAACATATCACTCACTTTGGTGATTTCACCGTAACTGTCTATGATTAATGAGGCGGTGGTTAACACTACCGCTTCGTTGTCTGGACAATCGTTCCTTTGTAATAGGTCCGAATCACAATGGAAGGTTCAAGAATGAAATACCGCATCGCTTTAGCTGTTTCTCTCTTTGCTCTTAGTGCCGGTAGTTATGCCACTACCCTGTGTCAGGAAAAGGAGCAAAATATCCTTAAGGAGA  >  NC_000913/3248804‑3249071                                                                                                                                        |                                                                                                                                     ctctCATCGCTTCGGATAACTTCCTGTTATCCGAAACATATCACTCACTTTGGTGATTTCACCGTAACTGTCTATGATTAATGAGGCGGTGGTTAACACTACCGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAgg                                                                                                                                   >  1:371963/1‑139 (MQ=255)      aTCGCTTCGGATAACTTCCTGTTATCCGAAACATATCACTCACTTTGGTGATTTCACCGTAACTGTCTATGATTAATGAGGCGGTGGTTAACACTACCGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGa                                                                                                                              >  2:946875/1‑139 (MQ=255)      aTCGCTTCGGATAACTTCCTGTTATCCGAAACATATCACTCACTTTGGTGATTTCACCGTAACTGTCTATGATTAATGAGGCGGTGGTTAACACTACCGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGa                                                                                                                              >  1:302531/1‑139 (MQ=255)        cGCTTCGGATAACTTCCTGTTATCCGAAACATATCACTCACTTTGGTGATTTCACCGTAACTGTCTATGATTAATGAGGCGGTGGTTAACACTACCGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAAt                                                                                                                            <  2:763890/139‑1 (MQ=255)            tCGGATAACTTCCTGTTATCCGAAACATATCACTCACTTTGGTGATTTCACCGTAACTGTCTATGATTAATGAGGCGGTGGTTAACACTACCGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAATc                                                                                                                           >  2:643513/1‑136 (MQ=255)            tCGGATAACTTCCTGTTATCCGAAACATATCACTCACTTTGGTGATTTCACCGTAACTGTCTATGATTAATGAGGCGGTGGTTAACACTACCGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAATc                                                                                                                           <  1:643513/136‑1 (MQ=255)               gATAACTTCCTGTTATCCGAAACATATCACTCACTTTGGTGATTTCACCGTAACTGTCTATGATTAATGAGGCGGTGGTTAACACTACCGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAATCACAATg                                                                                                                     <  2:908053/139‑1 (MQ=255)                    cTTCCTGTTATCCGAAACATATCACTCACTTTGGTGATTTCACCGTAACTGACTATGATTAATGAGGCGGTGGTTAACACTACCGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCTAATCACAATGGAAgg                                                                                                                <  1:675299/139‑1 (MQ=255)                     ttCCTGTTATCCGAAACATATCACTCACTTTGGTGATTTCACCGTAACTGTCTATGATTAATGAGGCGGTGGTTAACACTACCGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAATAACAATGTAAGGt                                                                                                               >  2:451305/1‑139 (MQ=255)                          gTTATCCGAAACATATCACTCACTTTGGTGATTTCACAGTAACAGTCTATGATTAATGAGGCGGTGGTTAACACTACCGCTTCGTTGTCTGGACAATCATTCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTCAAg                                                                                                          >  2:675299/1‑139 (MQ=255)                               ccGAAACATATCACTCACTTTGGTGATTTCACCGTAACTGTCTATGATTAATGAGGCGGTGGTTAACACTACCGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTCAAGAATGa                                                                                                     >  1:467079/1‑139 (MQ=255)                                            ctcactTTGGTGATTTCACCGTAACTGTCTATGATTAATGAGGCGGTGGTTAACACTACCGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTCAAGAATGAAATACCGCATCGc                                                                                        >  2:225636/1‑139 (MQ=255)                                                 tttGGTGATTTCACCGTAACTGTCTATGATTAATGAGGCGGTGGTTAACACTACCGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTCAAGAATGAAATACCGCATCGCTTTAg                                                                                   <  2:633503/139‑1 (MQ=255)                                                 tttGGTGATTTCACCGTAACTGTCTATGATTAATGAGGCGGTGGTTAACACTACCGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTCAAGAATGAAATACCGCATCGCTTTAg                                                                                   <  2:350682/139‑1 (MQ=255)                                                    ggTGATTTCACCGTAACTGTCTATGATTAATGAGGCGGTGGTTAACACTACCGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGa                                                                                                                   <  2:81615/104‑1 (MQ=255)                                                    ggTGATTTCACCGTAACTGTCTATGATTAATGAGGCGGTGGTTAACACTACCGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGa                                                                                                                   >  1:81615/1‑104 (MQ=255)                                                       gATTTCACCGTAACTGTCTATGATTAATGAGGCGGTGGTTAACACTACCGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGaa                                                                                                                             >  1:864119/1‑91 (MQ=255)                                                       gATTTCACCGTAACTGTCTATGATTAATGAGGCGGTGGTTAACACTACCGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGaa                                                                                                                             <  2:864119/91‑1 (MQ=255)                                                        aTTTCACCGTAACTGTCTATGATTAATGAGGCGGTGGTTAACACTACCGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTCAAGAATGAAATACCGCATCGCTTTAGCTGTTtc                                                                            <  1:946875/139‑1 (MQ=255)                                                          ttCACCGTAACTGTCTATGATTAATGAGGCGGTGGTTAACACTACCGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTCAAGAATGAAATACCGCATCGCTTTAGCTGTTtctc                                                                          >  2:912106/1‑139 (MQ=255)                                                           tCACCGTAACTGTCTATGATTAATGAGGCGGTGGTTAACACTACCGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTCAAGAATGAAATACCGCATCGCTTTAGCTGTTtctct                                                                         >  2:449835/1‑139 (MQ=255)                                                               cGTAACTGTCTATGATTAATGAGGCGGTGGTTAACACTACCGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTCAAGAATGAAATACCGCATCGCTTTAGCTGTTTCTCTCttt                                                                     <  2:870260/139‑1 (MQ=255)                                                                     tGTCTATGATTAATGAGGCGGTGGTTAACACTACCGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTCAAGAATGAAATACCGCATCGCTTTAGCTGTTTCTCTCTTTGCTCtt                                                               <  1:731533/139‑1 (MQ=255)                                                                      gTCTATGATTAATGAGGCGGTGGTTAACACTACCGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTCAAGAATGAAATACCGCATCGCTTTAGCTGTTTCTCTCTTTGCTCTTa                                                              <  1:225636/139‑1 (MQ=255)                                                                                 aTGAGGCGGTGGTTAACACTACCGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTCAAGAATGAAATACCGCATCGCTTTAGCTGTTTCTCTCTTTGCTCTTAGTGCCGGTAGt                                                   >  2:888807/1‑139 (MQ=255)                                                                                   gAGGCGGTGGTTAACACTACCGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTCAAGAATGAAATACCGCATCGCTTTAGCTGTTTCTCTCTTTGCTCTTAGTGCCGGTAGTTa                                                 <  2:467079/139‑1 (MQ=255)                                                                                      gCGGTGGTTAACACTACCGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTCAAGAATGAAATACCGCATCGCTTTAGCTGTTTCTCTCTTTGCTCTTAGTGCCGGTAGTTATGc                                              >  1:791604/1‑139 (MQ=255)                                                                                                   cTACCGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTCAAGAATGAAATACCGCATCGCTTTAGCTGTTTCTCTCTTTGCTCTTAGTGCCGGTAGTTATGCCACTACCCTgtgt                                 >  2:482380/1‑139 (MQ=255)                                                                                                    tACCGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTCAAGaa                                                                                                        <  2:563294/67‑1 (MQ=255)                                                                                                    tACCGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTCAAGaa                                                                                                        >  1:563294/1‑67 (MQ=255)                                                                                                    tACCGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTCAAGAATGAAATACCGCATCGCTTTAGCTGTTTCTCTCTTTGCTCTTAGTGCCGGTAGTTATGCCACTACCCTGTGTc                                >  1:668208/1‑139 (MQ=255)                                                                                                      ccGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTCAAGAATGAAATACCGCATCGCTTTAGCTGTTTCTCTCTTTGCTCTTAGTGCCGGTAGTTATGCCACTAcc                                       <  1:719550/130‑1 (MQ=255)                                                                                                      ccGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTCAAGAATGAAATACCGCATCGCTTTAGCTGTTTCTCTCTTTGCTCTTAGTGCCGGTAGTTATGCCACTAcc                                       >  2:719550/1‑130 (MQ=255)                                                                                                      ccGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTCAAGAATGAAATACCGCATCGCTTTAGCTGTTTCTCTCTTTGCTCTTAGTGCCGGTAGTTATGCCACTAcc                                       <  2:681917/130‑1 (MQ=255)                                                                                                      ccGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTCAAGAATGAAATACCGCATCGCTTTAGCTGTTTCTCTCTTTGCTCTTAGTGCCGGTAGTTATGCCACTACCCTGTGTCAg                              >  1:337484/1‑139 (MQ=255)                                                                                                      ccGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGCTCCGAATCACAATGGAAGGTTCAAGAATGAAATACCGCATCGCTTTAGCTGTTTCTCTCTGTGCTCTTAGTGCCGGTAGTTATGCCACTACCCTGTGTCAg                              >  2:675921/1‑139 (MQ=255)                                                                                                      ccGCTTCGTTGTCAGGACAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTCAAGACTGAAATACCGCATCGCTTTAGCTGTTTCTCTCTTTGCTCTTAGTGCCGGTAGTTATGCCACTAcc                                       >  1:681917/1‑130 (MQ=255)                                                                                                         cTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTCAAGAATGAAATACCGCATCGCTTTAGCTGTTTCTCTCTTTGCTCTTAGTGCCGGTAGTTATGCCa                                            <  1:661467/122‑1 (MQ=255)                                                                                                         cTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTCAAGAATGAAATACCGCATCGCTTTAGCTGTTTCTCTCTTTGCTCTTAGTGCCGGTAGTTATGCCACTACCCTGTGTCAGGaa                           >  2:884752/1‑139 (MQ=255)                                                                                                         cTTCGTTGTCTGGACAATCGCTCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTCAAGAATGAAATACCGCATCGCTTTAGCTGTTTCTCTCTTTGCTCTTAGTGCCGGTAGATATGCCa                                            >  2:661467/1‑122 (MQ=255)                                                                                                           tCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTc                                                                                                             >  1:117969/1‑55 (MQ=255)                                                                                                           tCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTc                                                                                                             <  2:117969/55‑1 (MQ=255)                                                                                                             gTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTCAAGAATGAAATACCGCATCGCTTTAGCTGTTTCTCTCTTTGCTCTTAGTGCCGGTAGTTATGCCACTACCCTGTGTCAGGAAAAgg                       <  2:337484/139‑1 (MQ=255)                                                                                                                    ggACAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTCAAGAATGAAATACCGCATCGCTTTAGCTGTTTCTCTCTTTGCTCTTAGTGCCGGTAGTTATGCCACTACCCTGTGTCAGGAAAAGGAGCaaaa                <  1:482380/139‑1 (MQ=255)                                                                                                                      aCAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTCAAGAATGAAATACCGCATCGCTTTAGCTGTTTCTCTCTTTGCTCTTAGTGCCGGTAGTTATGCCACTACCCTGTGTCAGGAAAAGGAGCAAAata              <  2:668208/139‑1 (MQ=255)                                                                                                                        aaTCGTTCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTCAAGAATGAAATACCGCATCGCTTTAGCTGTTtc                                                                            <  2:452020/75‑1 (MQ=255)                                                                                                                        aaTCGTTCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTCAAGAATGAAATACCGCATCGCTTTAGCTGTTtc                                                                            >  1:452020/1‑75 (MQ=255)                                                                                                                         aTCGTTCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTCAAGAATGAAATACCGCATCGCTTTAGCTGTTTCTCTCTTTGCTCTTAGTGCCGGTAGTTATGCCACTACCCTGTGTCAGGAAAAGGAGCAAAATATcc           >  2:550048/1‑139 (MQ=255)                                                                                                                          tCGTTCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTCAAGAATGATATACCGCATCGCTTTAGCTGTTTCTCTCTTTGCTCTTAGTGCCGGTAGTTATGCCACTACCCTGTGTCAGGAAAAGGAGCAAAATATCCt          >  1:236943/1‑139 (MQ=255)                                                                                                                           cGTTCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTCAAGAATGAAATACCGCAt                                                                                           >  2:113247/1‑57 (MQ=255)                                                                                                                           cGTTCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTCAAGAATGAAATACCGCAt                                                                                           <  1:113247/57‑1 (MQ=255)                                                                                                                            gTTCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTCAAGAATGAAATACCGCATCGCTTTAGCTGTTTCTCTCTTTGCTCTTAGTGCCGGTAGTTATGCCACTACCCTGTGTCAGGAAAAGTAGCAAAATATCCTTa        <  1:675921/139‑1 (MQ=255)                                                                                                                                  ttGTAACAGGTCCGAATCACAATGGAAGGTTCAAGAATGAAATACCGCATCGCTTTAGCTGTTTCTCTCTTTGCTCTTAGTGCCGGTAGTTATGCCACTACCCTGTGTCAGGAAAAGGAGCAAAATATCCTTAAGgaga  <  2:302531/139‑1 (MQ=255)                                                                                                                                        |                                                                                                                                     CTCTCATCGCTTCGGATAACTTCCTGTTATCCGAAACATATCACTCACTTTGGTGATTTCACCGTAACTGTCTATGATTAATGAGGCGGTGGTTAACACTACCGCTTCGTTGTCTGGACAATCGTTCCTTTGTAATAGGTCCGAATCACAATGGAAGGTTCAAGAATGAAATACCGCATCGCTTTAGCTGTTTCTCTCTTTGCTCTTAGTGCCGGTAGTTATGCCACTACCCTGTGTCAGGAAAAGGAGCAAAATATCCTTAAGGAGA  >  NC_000913/3248804‑3249071

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑