Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,269,012	A→G	S200G (AGT→GGT)	yhaC →	pentapetide repeats‑related protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,269,012	0	A	G	100.0%	197.6 / NA	55	S200G (AGT→GGT)	yhaC	pentapetide repeats‑related protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (27/28);  total (27/28)

TGGACTTACGTAATGTCGATTTAGATTATTATGATTTCACAGATAAACATATGGCTAATACTATTTTAAATCCTTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTAGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTTAATAAGCATTAGCGATATGTATTCTGAAGAAAAAATCAAGTTTGTCGAAAGTTGTTTAAATGAGCCTGGAGACAT  >  NC_000913/3268881‑3269145                                                                                                                                    |                                                                                                                                      tGGACTTACGTAATGTCGATTTAGATTATTATGATTTCACAGATAAACATATGGCTAATACTATTTTAAATCCTTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTGGTTCAac                                                                                                                                >  1:543025/1‑139 (MQ=255) tGGACTTACGTAATGTCGATTTAGATTATTATGATTTCACAGATAAACATATGGCTAATACTATTTTAAATCCTTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTGGTTCAac                                                                                                                                >  2:38071/1‑139 (MQ=255)         cGTAATGTCGATTTAGATTATTATGATTTCACAGATAAACATATGGCTAATACTATTTTAAATCCTTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTGGTTCAACACAAAACg                                                                                                                        >  2:855299/1‑139 (MQ=255)         cGTAATGTCGATTTAGATTATTATGATTTCACAGATAAACATATGGCTAATACTATTTTAAATCCTTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTGGTTCAACACAAAACg                                                                                                                        >  1:478647/1‑139 (MQ=255)              tGTCGATTTAGATTATTATGATTTCACAGATAAACATATGGCTAATACTATTTTAAATCCTTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTGGTTCAACACAAAACGCcaca                                                                                                                   <  2:543025/139‑1 (MQ=255)                   aTTTAGATTATTATGATTTCACAGATAAACATATGGCTAATACTATTTTAAATCCTTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTGGTTCAACACAAAACGCCACAAtctc                                                                                                              >  2:881157/1‑139 (MQ=255)                    tttAGATTATTATGATTTCACAGATAAACATATGGCTAATACTATTTTAAATCCTTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTGGTTCAACACAAAACGCCACAATCTcc                                                                                                             <  2:721970/139‑1 (MQ=255)                    tttAGATTATTATGATTTCACAGATAAACATATGGCTAATACTATTTTAAATCCTTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTGGTTCAACACAAAACGCCACAATCTcc                                                                                                             <  2:308283/139‑1 (MQ=255)                    tttAGATTATTATGATTTCACAGATAAACATATGGCTAATACTATTTTAAATCCTTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTGGTTCAACACAAAACGCCACAATCTcc                                                                                                             <  2:462958/139‑1 (MQ=255)                         attattATGATTTCACAGATAAACATATGGCTAATACTATTTTAAATCCTTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTGGTTCAACACAAAACGCCACAATCTCCTGGGa                                                                                                        <  2:478647/139‑1 (MQ=255)                             ttatGATTTCACAGATAAACATATGGCTAATACTATTTTAAATCCTTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTGGTTCAACACAAAACGCCACAATCTCCTGGGattat                                                                                                    <  1:881157/139‑1 (MQ=255)                                  aTTTCACAGATAAACATATGGCTAATACTATTTTAAATCCTTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTGGTTCAACACAAAACGCcaca                                                                                                                   >  1:275000/1‑119 (MQ=255)                                  aTTTCACAGATAAACATATGGCTAATACTATTTTAAATCCTTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTGGTTCAACACAAAACGCcaca                                                                                                                   <  2:275000/119‑1 (MQ=255)                                           aTAAACATATGGCTAATACTATTTTAAATTCTTTTAAATTGAATTAAACAAATTTTACTAATGCCAAGACGTTTCAGGTTAATTTTGTTGGTTCAACACAAAACGCCACAATATCCTAGGATTATTTACTAAAAATAAc                                                                                      >  1:589322/1‑139 (MQ=255)                                            tAAACATATGGCTAATACTATTTTAAATCCTTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTGGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACg                                                                                     <  2:909374/139‑1 (MQ=255)                                             aaaCATATGGCTAATACTATTTTAAATCCTTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTGGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGc                                                                                    <  2:456332/139‑1 (MQ=255)                                             aaaCATATGGCTAATACTATTTTAAATCCTTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTGGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGc                                                                                    <  1:38071/139‑1 (MQ=255)                                             aaaCATATGGCTAATACTATTTTAAATCCTTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTGGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGc                                                                                    >  1:921554/1‑139 (MQ=255)                                                   atGGCTAATACTATTTTAAATCCTTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTGGTTCAACACAAAACGCcaca                                                                                                                   >  2:310134/1‑102 (MQ=255)                                                   atGGCTAATACTATTTTAAATCCTTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTGGTTCAACACAAAACGCcaca                                                                                                                   <  1:310134/102‑1 (MQ=255)                                                     ggCTAATACTATTTTAAATCCTTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTGGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTta                                                                            >  1:506723/1‑139 (MQ=255)                                                     ggCTAATACTATTTTAAATCCTACTAAATTGAATTCAACAAATTTTACTCATGCCAACATGTTTCAGGTTAATTTTGTTGGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTta                                                                            >  1:311183/1‑139 (MQ=255)                                                             cTATTTTAAATCCTTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTGGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTTAATAAGCAt                                                                    <  1:260898/139‑1 (MQ=255)                                                                ttttAAATCCTTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTGGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTTAATAAGCa                                                                     >  1:82130/1‑135 (MQ=255)                                                                ttttAAATCCTTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTGGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTTAATAAGCa                                                                     <  2:82130/135‑1 (MQ=255)                                                                ttttAAATCCTTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTGGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTTAATAAGCATTAg                                                                 >  1:258414/1‑139 (MQ=255)                                                                 tttAAATCCTTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTGGTTCAACACAAAACGCcaca                                                                                                                   <  1:457682/88‑1 (MQ=255)                                                                 tttAAATCCTTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTGGTTCAACACAAAACGCcaca                                                                                                                   >  2:457682/1‑88 (MQ=255)                                                                     aaTCCTTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTGGTTCAACACAAAACGCCACAATCTCCTGGGa                                                                                                        >  2:401157/1‑95 (MQ=255)                                                                     aaTCCTTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTGGTTCAACACAAAACGCCACAATCTCCTGGGa                                                                                                        <  1:401157/95‑1 (MQ=255)                                                                      aTCCTTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTGGTTCAACACAAAACGCcaca                                                                                                                   <  2:387366/83‑1 (MQ=255)                                                                      aTCCTTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTGGTTCAACACAAAACGCcaca                                                                                                                   >  1:387366/1‑83 (MQ=255)                                                                          ttttAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTGGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTTAATAAGCATTAGCGATATGTAt                                                       >  2:864671/1‑139 (MQ=255)                                                                                   gAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTGGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTTAATAAGCa                                                                     >  2:782938/1‑116 (MQ=255)                                                                                   gAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTGGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTTAATAAGCa                                                                     <  1:782938/116‑1 (MQ=255)                                                                                    aaTTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTGGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTtaat                                                                          >  1:404307/1‑110 (MQ=255)                                                                                    aaTTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTGGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTtaat                                                                          <  2:404307/110‑1 (MQ=255)                                                                                             aaTTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTGGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTTAATAAGCATTAGCGATATGTATTCTGAAGAAAAAATCAAGt                                    <  1:864671/139‑1 (MQ=255)                                                                                              aTTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTGGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGc                                                                                    >  2:626011/1‑90 (MQ=255)                                                                                              aTTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTGGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGc                                                                                    <  1:626011/90‑1 (MQ=255)                                                                                              aTTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTGGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTTAATAAGCATTAGCGATATGTATTCTGAAGAAAAAATCAAGtt                                   >  1:116444/1‑139 (MQ=255)                                                                                                tttACTAATGCCAACATGTTTCAGGTTAATTTTGTTGGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTTAATAAGCAt                                                                    >  2:970707/1‑104 (MQ=255)                                                                                                tttACTAATGCCAACATGTTTCAGGTTAATTTTGTTGGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTTAATAAGCAt                                                                    <  1:970707/104‑1 (MQ=255)                                                                                                     tAATGCCAACATGTTTCAGGTTAATTTTGTTGGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTTAATAAGCATTAGCGATATGTATTCTGAAGAAAAAATCAAGTTTGTCGaa                            <  1:319645/139‑1 (MQ=255)                                                                                                       aTGCCAATTTGTTTCAGTTTAATTTGGTTGATTAAACACAAAACGCCACAATCTCCTGGCATTATTTACTAATAATAACGCCTTTTTTAATAAGCATTTGCGATATGTAATCTGAAGAAAAAATCAAGTTAGTAGAAAg                          <  1:760925/139‑1 (MQ=255)                                                                                                          ccAACATGTTTCAGGTTAATTTTGTTGGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTTAATAAGCATTAGCGATATGTATTCTGAAGAAAAAATCAAGTTTGTCGAAAgttg                       >  2:95315/1‑139 (MQ=255)                                                                                                                tGTTTCAGGTTAATTTTGTTGGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTTAATAAGCATTAGCGATATGTATTCTGAAGAAAAAATCAAGTTTGTCGAAAGTTGTTTaaa                 <  2:116444/139‑1 (MQ=255)                                                                                                                   ttCAGGTTAATTTTGTTGGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTTAATAAGCATTAGCGATATGTATTCTGAAGAAAAAATCAAGTTTGTCGAAAGTTGTTTAAATGa              >  2:30127/1‑139 (MQ=255)                                                                                                                       ggTTAATTTTGTTGGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTTAATAAGCATTAGCGATATGTATTCTGAAGAAAAAATCAAGTTTGTCGAAAg                          >  2:441869/1‑123 (MQ=255)                                                                                                                       ggTTAATTTTGTTGGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTTAATAAGCATTAGCGATATGTATTCTGAAGAAAAAATCAAGTTTGTCGAAAg                          <  1:441869/123‑1 (MQ=255)                                                                                                                        gTTAATTTTGTTGGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTTAATAAGCATTAGCGATATGTATTCTGAAGAAAAAATCAAGTTTGTCGAAAGTTGTTTAAATGAGCCt          >  2:706993/1‑138 (MQ=255)                                                                                                                        gTTAATTTTGTTGGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTTAATAAGCATTAGCGATATGTATTCTGAAGAAAAAATCAAGTTTGTCGAAAGTTGTTTAAATGAGCCt          <  1:706993/138‑1 (MQ=255)                                                                                                                            aTTTTGTTGGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTTAATAAGCATTAGCGATATGTATTCTGAAGAAAAAATCAAGTTTGTCGAAAGTTGTTTAAATGAGCCTGgaga     <  2:921554/139‑1 (MQ=255)                                                                                                                             ttttGTTGGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTTAATAAGCATTAGCGATATGTATTCTGAAGAAAAAATCAAGt                                    <  2:644012/107‑1 (MQ=255)                                                                                                                             ttttGTTGGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTTAATAAGCATTAGCGATATGTATTCTGAAGAAAAAATCAAGt                                    >  1:644012/1‑107 (MQ=255)                                                                                                                               ttgttgGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTTAATAAGCATTAGCGATATGTATTCTGAAGAAAAAATCAAGTTTGTCGAAAGTTGTTTAAATGAGCCTGGAGACAt  <  2:258414/139‑1 (MQ=255)                                                                                                                                    |                                                                                                                                      TGGACTTACGTAATGTCGATTTAGATTATTATGATTTCACAGATAAACATATGGCTAATACTATTTTAAATCCTTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTAGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTTAATAAGCATTAGCGATATGTATTCTGAAGAAAAAATCAAGTTTGTCGAAAGTTGTTTAAATGAGCCTGGAGACAT  >  NC_000913/3268881‑3269145

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑