Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,478,592 C→T *67* (TAG→TAA)  yheV ← DUF2387 family putative metal‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,478,5920CT100.0% 163.1 / NA 51*67* (TAG→TAA) yheVDUF2387 family putative metal‑binding protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (23/28);  total (23/28)

ATAGGCCAGGCTGACCACCAGGTCTTTTGCTACTTTCATGATATCTCCTGAGCAT‑GGGAAGAATAGTGGCGCAGATTGTAGCGGAATTCTGCAGCCGTGTACTCACTAGCTTAAAAAAACTCGGCGCATATCGCTAGTCCGGATGAAAAATCCCGATCACTTGCTCATCTTTGCGAACGTGATCGCGGGCTTCTTTGTCTGCTTCTCGCATCTGATGTCCGCACTTAACACATTCAACAATATCAATATTATTTTCGCGCCACA  >  NC_000913/3478459‑3478722
                                                                                                                                      |                                                                                                                                  
aTAGGCCAGGCTGACCACCAGGTCTTTTGCTACTTTCATGATATCTCCTGAGCAT‑GGGAAGAATAGTGGCGCAGATTGTAGCGGAATTCTGCAGCCGTGTACTCACTAGCTTAAAAAAACTCGGCGCATATCGTTAGTc                                                                                                                               >  2:914313/1‑139 (MQ=255)
   ggCCAGGCTGACCACCAGGTCTTTTGCTACTTTCATGATATCTCCTGAGCAT‑GGGAAGAATAGTGGCGCAGATTGTAGCGGAATTCTGCAGCCGTGTACTCACTAGCTTAAAAAAACTCGGCGCATATCGTTAGTCCgg                                                                                                                            <  1:384081/139‑1 (MQ=255)
       aGGCTGACCACCAGGTCTTTTGCTACTTTCATGATATCTCCTGAGCAT‑GGGAAGAATAGTGGCGCAGATTGTAGCGGAATTCTGCAGCCGTGTACTCACTAGCTTAAAAAAACTCGGCGCATATCGTTAGTCCGGATGa                                                                                                                        >  1:477940/1‑139 (MQ=255)
         gCTGACCACCAGGTCTTTTGCTACTTTCATGATATCTCCTGAGCAT‑GGGAAGAATAGTGGCGCAGATTGTAGCGGAATTCTGCAGCCGTGTACTCACTAGCTTAAAAAAACTCGGCGCATATCGTTAGTCCGGATGaaa                                                                                                                      >  2:816990/1‑139 (MQ=255)
         gCTGACCACCAGGTCTTTTGCTACTTTCATGATATCTCCTGAGCAT‑GGGAAGAATAGTGGCGCAGATTGTAGCGGAATTCTGCAGCCGTGTACTCACTAGCTTAAAAAAACTCGGCGCATATCGTTAGTCCGGATGaaa                                                                                                                      >  2:130613/1‑139 (MQ=255)
          cgtaccaccATGTCTTTTGCTACTTTCATGATATCTCCTGATCAT‑GGGAAGAATAGTGGCGCAGATTGTAGCGGAATTCTGCAGCCGTGTACTCACTAGCTTAAAAAAACTCGGCGCATATCGTTAGTCCGGATGaaaa                                                                                                                     <  2:635274/136‑1 (MQ=255)
              ccaccaGGTCTTTTGCTACTTTCATGATATCTCCTGAGCATGGGGAAGAATAGTGGCGCAGATTGTAGCGGAATTCTGCAGCCGTGTACTCACTAGCTTAAAAAAACTCGGCGCATATCGTTAGTCCGGATGAAAAATc                                                                                                                  >  2:660065/1‑139 (MQ=255)
                    ggTCTTTTGCTACTTTCATGATATCTCCTGAGCAT‑GGGAAGAATAGTGGCGCAGATTGTAGCGGAATTCTGCAGCCGTGTACTCACTAGCTTAAAAAAACTCGGCGCATATCGTTAGTc                                                                                                                               >  2:7194/1‑119 (MQ=255)
                    ggTCTTTTGCTACTTTCATGATATCTCCTGAGCAT‑GGGAAGAATAGTGGCGCAGATTGTAGCGGAATTCTGCAGCCGTGTACTCACTAGCTTAAAAAAACTCGGCGCATATCGTTAGTc                                                                                                                               <  1:7194/119‑1 (MQ=255)
                     gTCTTTTGCTACTTTCATGATATCTCCTGAGCAT‑GGGAAGAATAGTGGCGCAGATTGTAGCGGAATTCTGCAGCCGTGTACTCACTAGCTTAAAAAAACTCGGCGCATATCGTTAGTCCGGATGAAAAATCCCGATCAc                                                                                                          <  1:914313/139‑1 (MQ=255)
                            gCTACTTTCATGATATCTCCTGAGCAT‑GGGAAGAATAGTGGCGCAGATTGTAGCGGAATTCTGCAGCCGTGTACTCACTAGCTTAAAAAAACTCGGCGCATATCGTTAGTCCGGATGAAAAATCCCGATCACTTGCTCa                                                                                                   >  1:867484/1‑139 (MQ=255)
                               aCTTTCATGATATCTCCTGAGCAT‑GGGAAGAATAGTGGCGCAGATTGTAGCGGAATTCTGCAGCCGTGTACTCACTAGCTTAAAAAAACTCGGCGCATATCGTTAGTCCGGATGAAAAATCCCGATCACTTGCTCATCt                                                                                                >  2:749061/1‑139 (MQ=255)
                                cTTTCATGATATCTCCTGAGCAT‑GGGAAGAATAGTGGCGCAGATTGTAGCGGAATTCTGCAGCCGTGTACTCACTAGCTTAAAAAAACTCGGCGCATATCGTTAGTCCGGATGAAAAATCCCGATCACTTGCTCATCtt                                                                                               >  2:410753/1‑139 (MQ=255)
                                              ccTGAGCAT‑GGGAAGAATAGTGGCGCAGATTGTAGCGGAATTCTGCAGCCGTGTACTCACTAGCTTAAAAAAACTCGGCGCATATCGTTAGTCCGGATGAAAAATCCCGATCACTTGCTCATCTTTGCGAACGTGATcg                                                                                 <  1:410753/139‑1 (MQ=255)
                                               cTGAGCAT‑GGGAAGAATAGTGGCGCAGATTGTAGCGGAATTCTGCAGCCGTGTACTCACTAGCTTAAAAAAACTCGGCGCATATCGTTAGTCCGGATGAAAAATCCCGATCACTTGCTCATCTTTGCGAACGTGATcgc                                                                                >  2:86868/1‑139 (MQ=255)
                                                tGAGCAT‑GGGAAGAATAGTGGCGCAGATTGTAGCGGAATTCTGCAGCCGTGTACTCACTAGCTTAAAAAAACTCGGCGCATATCGTTAGTCCGGATGAAAAATCCCGATCACTTGCTCATCTTTGCGAACGTGATcgcg                                                                               >  2:979960/1‑139 (MQ=255)
                                                 gAGCAT‑GGGAAGAATAGTGGCGCAGATTGTAGCGGAATTCTGCAGCCGTGTACTCACTAGCTTAAAAAAACTCGGCGCATATCGTTAGTCCGGATGAAAAATCCCGATCACTTGCTCATCTTTGCGAACGTGATCGCgg                                                                              <  2:771193/139‑1 (MQ=255)
                                                        gggAAGAATAGTGGCGCAGATTGTAGCGGAATTCTGCAGCCGTGTACTCACTAGCTTAAAAAAACTCGGCGCATATCGTTAGTCCGGATGAAAAATCCCGATCACTTGCTCATCTTTGCGAACGTGATCGCGGGcttct                                                                        >  1:773630/1‑139 (MQ=255)
                                                          gaagaaTAGTGGCGCAGATTGTAGCGGAATTCTGCAGCCGTGTACTCACTAGCTTAAAAAAACTCGGCGCATATCGTTAGTCCGGATGAAAAATCCCGATCACTTGCTCATCTTTGCGAACGTGATCGCGGGCTTCttt                                                                      >  1:318357/1‑139 (MQ=255)
                                                              aaTAGTGGCGCAGATTGTAGCGGAATTCTGCAGCCGTGTACTCACTAGCTTAAAAAAACTCGGCGCATATCGTTAGTCCGGATGAAAAATCCCGATCACTTGCTCATCTTTGCGAAc                                                                                        >  1:326646/1‑117 (MQ=255)
                                                              aaTAGTGGCGCAGATTGTAGCGGAATTCTGCAGCCGTGTACTCACTAGCTTAAAAAAACTCGGCGCATATCGTTAGTCCGGATGAAAAATCCCGATCACTTGCTCATCTTTGCGAAc                                                                                        <  2:326646/117‑1 (MQ=255)
                                                                    ggCGCAGATTGTAGCGGAATTCTGCAGCCGTGTACTCACTAGCTTAAAAAAACTCGGCGCATATCGTTAGTCCGGATGAAAAATCCCGATCACTTGCTCATCTTTGCGAACGTGATCGCGGGCTTCTTTGTCTGCTtct                                                            <  2:477940/139‑1 (MQ=255)
                                                                      cgcAGATTGTAGCGGAATTCTGCAGCCGTGTACTCACTAGCTTAAAAAAACTCGGCGCATATCGTTAGTCCGGATGAAAAATCCCGATCACTTGCTCATCTTTGCGAACGTGATCGCGGGCTTCTTTGTCTGCTTCTCg                                                          <  1:816990/139‑1 (MQ=255)
                                                                               tAGCGGAATTCTGCAGCCGTGTACTCACTAGCTTAAAAAAACTCGGCGCATATCGTTAGTCCGGATGaaaaa                                                                                                                    <  2:214261/72‑1 (MQ=255)
                                                                               tAGCGGAATTCTGCAGCCGTGTACTCACTAGCTTAAAAAAACTCGGCGCATATCGTTAGTCCGGATGaaaaa                                                                                                                    >  1:214261/1‑72 (MQ=255)
                                                                                aGCGGAATTCTGCAGCCGTGTACTCACTAGCTTAAAAAAACTCGGCGCATATCGTTAGTCCGGATGaaaa                                                                                                                     >  2:761769/1‑70 (MQ=255)
                                                                                aGCGGAATTCTGCAGCCGTGTACTCACTAGCTTAAAAAAACTCGGCGCATATCGTTAGTCCGGATGaaaa                                                                                                                     <  1:761769/70‑1 (MQ=255)
                                                                                aGCGGAATTCTGCAGCCGTGTACTCACTAGCTTAAAAAAACTCGGCGCATATCGTTAGTCCGGATGAAAAATCCCGATCACTTGCTCATCTTTGCGAACGTGATCGCGGGCTTCTTTGTCTGCTTCTCGCATCTGATGt                                                <  1:749061/139‑1 (MQ=255)
                                                                                aGCGGAATTCTGCAGCCGTGTACTCACTAGCTTAAAAAAACTCGGCGCATATCGTTAGTCCGGATGAAAAATCCCGATCACTTGCTCATCTTTGCGAACGTGATCGCGGGCTTCTTTGTCTGCTTCTCGCATCTGATGt                                                <  2:318357/139‑1 (MQ=255)
                                                                                 gCGGAATTCTGCAGCCGTGTACTCACTAGCTTAAAAAAACTCGGCGCATATCGTTAGTCCGGATGAAAAATCCCGATCACTTGCTCATCTTTGCGAACGTGATCGCGGGCTTCTTTGTCTGCTTCTCGCATCTGATGTc                                               <  1:660065/139‑1 (MQ=255)
                                                                                     aaTTCTGCAGCCGTGTACTCACTAGCTTAAAAAAACTCGGCGCATATCGTTAGTCCGGATGAAAAATCCCGATCACTTGCTCATCTTTGCGAACGTGATCGCGGGCTTCTTTGTCTGCTTCTCGCATCTGATGTCCGCa                                           <  2:867484/139‑1 (MQ=255)
                                                                                      aTTCTGCAGCCGTGTACTCACTAGCTTAAAAAAACTCGGCGCATATCGTTAGTCCGGATGAAAAATCCCGATCACTTGCTCATCTTTGCGAACGTGATCGCGGGCTTCTTTGTCTGCTTCTCGCATCTGATGTCCGCAc                                          <  1:86868/139‑1 (MQ=255)
                                                                                       ttCTGCAGCCGTGTACTCACTAGCTTAAAAAAACTCGGCGCATATCGTTAGTCCGGATGAAAAATCCCGATCACTTGCTCATCTTTGCGAACGTGATCGCGGGCTTCTTTGTCTGCTTCTCGCATCTGATGTCCGCACt                                         >  2:596965/1‑139 (MQ=255)
                                                                                           gCAGCCGTGTACTCACTAGCTTAAAAAAACTCGGCGCATATCGTTAGTCCGGATGAAAAATCCCGATCACTTGCTCATCTTTGCGAACGTGATCGCGGGCTTCTTTGTCTGCTTCTCGCATCTGATGTCCGCACTTAac                                     <  2:773630/139‑1 (MQ=255)
                                                                                              gCCGTGTACTCACTAGCTTAAAAAAACTCGGCGCATATCGTTAGTCCGGATGAAAAATCCCGATCACTTGCTCATCTTTGCGAACGTGATCGCGGGCTTCTTTGTCTGCTTCTCGCATCTGATGTCCGCACTTAAcaca                                  <  2:53271/139‑1 (MQ=255)
                                                                                                cGTGTACTCACTAGCTTAAAAAAACTCGGCGCATATCGTTAGTCCGGATGAAAAATCCCGATCACTTGCTCATCTTTGCGAACGTGATCGCGGGCTTCTTTGTCTGCTTCTCGCATCTGATGTCCGCACTTAACACAtt                                <  1:979960/139‑1 (MQ=255)
                                                                                                 gtgtACTCACTAGCTTAAAAAAACTCGGCGCATATCGTTAGTCCGGATGAAAAATCCCGATCACTTGCTCATCTTTGCGAACGTGATCGCGGGCTTCTTTGTCTGCTTCTCGCATCTGATGTCCGCACTTAACACATTc                               >  1:757295/1‑139 (MQ=255)
                                                                                                  tgtACTCACTAGCTTAAAAAAACTCGGCGCATATCGTTAGTCCGGATGAAAAATCCCGATCACTTGCTCATCTTTGCGAACGTGATcgc                                                                                <  1:183038/89‑1 (MQ=255)
                                                                                                  tgtACTCACTAGCTTAAAAAAACTCGGCGCATATCGTTAGTCCGGATGAAAAATCCCGATCACTTGCTCATCTTTGCGAACGTGATcgc                                                                                >  2:183038/1‑89 (MQ=255)
                                                                                                    tACTCACTAGCTTAAAAAAACTCGGCGCATATCGTTAGTCCGGATGAAAAATCCCTATCACTTGCTCATCTTTGCGAACGTGATCGCGGGCTTCTTTGTCTGCTTCTCGCATCTGATGTCCGCACTTAACACATTcaac                            <  2:757295/139‑1 (MQ=255)
                                                                                                    tACTCACTAGCTTAAAAAAACTCGGCGCATATCGTTAGTCCGGATGAAAAATCCCGATCACTTGCTCATCTTTGCGAACGTGATCGCGGGCTTCTTTGTCTGCTTCTCGCATCTGATGTCCGCACTTAACACATTcaac                            <  1:950190/139‑1 (MQ=255)
                                                                                                           tAGCTTAAAAAAACTCGGCGCATATCGTTAGTCCGGATGAAAAATCCCGATCACTTGCTCATCTTTGCGAACGTGATCGCGGGCTTCTTTGTCTGCTtct                                                            >  1:494851/1‑100 (MQ=255)
                                                                                                           tAGCTTAAAAAAACTCGGCGCATATCGTTAGTCCGGATGAAAAATCCCGATCACTTGCTCATCTTTGCGAACGTGATCGCGGGCTTCTTTGTCTGCTtct                                                            <  2:494851/100‑1 (MQ=255)
                                                                                                              cTTAAAAAAACTCGGCGCATATCGTTAGTCCGGATGAAAAATCCCGATCACTTGCTCATCTTTGCGAACGTGATCGCGGGCTTCTTTGTCTGCTTCTCGCATCTGATGTCCGCACTTaa                                      >  1:205212/1‑119 (MQ=255)
                                                                                                              cTTAAAAAAACTCGGCGCATATCGTTAGTCCGGATGAAAAATCCCGATCACTTGCTCATCTTTGCGAACGTGATCGCGGGCTTCTTTGTCTGCTTCTCGCATCTGATGTCCGCACTTaa                                      <  2:205212/119‑1 (MQ=255)
                                                                                                                        cTCGGCGCATATCGTTAGTCCGGATGAAAAATCCCGATCACTTGCTCATCTTTGCGAACGTGATCGCGGGCTTCTTTGTCTGCTTCTCGCATCTGATGTcc                                              <  1:386417/101‑1 (MQ=255)
                                                                                                                        cTCGGCGCATATCGTTAGTCCGGATGAAAAATCCCGATCACTTGCTCATCTTTGCGAACGTGATCGCGGGCTTCTTTGTCTGCTTCTCGCATCTGATGTcc                                              >  2:386417/1‑101 (MQ=255)
                                                                                                                         tCGGCGCATATCGTTAGTCCGGATGAAAAATCCCGATCACTTGCTCATCTTTGCGAACGTGATCGCGGGCTTCTTTGTCTGCTTCTCGCATCTGATGTCCGCACTTAACACATTCAACAATATCAATATTATTTTcgcg       <  1:893048/139‑1 (MQ=255)
                                                                                                                             cgcATATCGTTAGTCCGGATGAAAAATCCCGATCACTTGCTCATCTTTGCGAACGTGATCGCGGGCTTCTTTGTCTGCTTCTCGCATCTGATGTcc                                              >  1:590852/1‑96 (MQ=255)
                                                                                                                             cgcATATCGTTAGTCCGGATGAAAAATCCCGATCACTTGCTCATCTTTGCGAACGTGATCGCGGGCTTCTTTGTCTGCTTCTCGCATCTGATGTcc                                              <  2:590852/96‑1 (MQ=255)
                                                                                                                              gcATATCGTTAGTCCGGATGAAAAATCCCGATCACTTGCTCATCTTTGCGAACGTGATCGCGGGCTTCTTTGTCTGCTTCTCGCATCTGATGTCCGCACTTAACACATTCAACAATATCAATATTATTTTCGCGCcaca  <  1:596965/139‑1 (MQ=255)
                                                                                                                                      |                                                                                                                                  
ATAGGCCAGGCTGACCACCAGGTCTTTTGCTACTTTCATGATATCTCCTGAGCAT‑GGGAAGAATAGTGGCGCAGATTGTAGCGGAATTCTGCAGCCGTGTACTCACTAGCTTAAAAAAACTCGGCGCATATCGCTAGTCCGGATGAAAAATCCCGATCACTTGCTCATCTTTGCGAACGTGATCGCGGGCTTCTTTGTCTGCTTCTCGCATCTGATGTCCGCACTTAACACATTCAACAATATCAATATTATTTTCGCGCCACA  >  NC_000913/3478459‑3478722

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑