Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,503,952	G→A	*262* (TAG→TAA)	frlD →	fructoselysine 6‑kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,503,952	0	G	A	100.0%	90.9 / NA	29	*262* (TAG→TAA)	frlD	fructoselysine 6‑kinase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (14/15);  total (14/15)

CGACACCATGGGTGCCGGAGATTCGTTCATTGCCGGATTCCTTTGCGGCTGGTCTGCGGGGATGACATTACCGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCT‑GGTAGGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGAGTAATTCATTCTTTATTTCAGGGTCGATTATGTCAGCTACGGACCGCTACT  >  NC_000913/3503817‑3504073                                                                                                                                         |                                                                                                                          cGACACCATGGGTGCCGGAGATTCGTTCATTGCCGGATTCCTTTGCGGCTGGTCTGCGGGGATGACATTACCGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCT‑ggtaagta                                                                                                                        <  1:257131/139‑1 (MQ=255)       cATGGGTGCCGGAGATTCGTTCATTGCCGGATTCCTTTGCGGCTGGTCTGCGGGGATGACATTACCGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCT‑GGTAAGTATAACGt                                                                                                                  >  1:790844/1‑139 (MQ=255)         tGGGTGCCGGAGATTCGTTCATTGCCGGATTCCTTTGCGGCTGGTCTGCGGGGATGACATTACCGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCT‑GGTAAGTATAACGTTg                                                                                                                >  1:538456/1‑139 (MQ=255)            gTGCCGGAGATTCGTTCATTGCCGGATTCCTTTGCGGCTGGTCTGCGGGGATGACATTACCGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCT‑GGTAAGTATAACGTTGGCg                                                                                                             <  2:538456/139‑1 (MQ=255)                cGGAGATTCGTTCATTGCCGGATTCCTTTGCGGCTGGTCTGCGGGGATGACATTACCGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCT‑GGTAAGTATAACGTTGGCGTGAg                                                                                                         <  2:790844/139‑1 (MQ=255)                             aTTGCCGGATTCCTTTGCGGCTGGTCTGCGGGGATGACATTACCGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCT‑GGTAAGTATAACGTTGGCGTGAGCATCTTCACGCCa                                                                                            <  1:246491/139‑1 (MQ=255)                               tGCCGGATTCCTTTGCGGCTGGTCTGCGGGGATGACATTACCGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCT‑GGTAAGTATAACGTTGGCGTGAGCATCTTCACGCCAAc                                                                                          <  1:922614/139‑1 (MQ=255)                                         cTTTGCGGCTGGTCTGCGGGGATGACATTACCGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCT‑GGTAAGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTAc                                                                                >  1:581863/1‑139 (MQ=255)                                                        gCGGGGATGACATTACCGCAGGTGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCT‑GGTAAGTATAACGTTGGCGTGAGCATCt                                                                                                    >  1:985127/1‑104 (MQ=255)                                                        gCGGGGATGACATTACCGCAGGTGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCT‑GGTAAGTATAACGTTGGCGTGAGCATCt                                                                                                    <  2:985127/104‑1 (MQ=255)                                                         cGGGGATGACATTACCGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCT‑GGTAAGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGaaaa                                                                     <  1:912259/134‑1 (MQ=255)                                                         cGGGGATGACATTACCGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCT‑GGTAAGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGaaaa                                                                     >  2:912259/1‑134 (MQ=255)                                                              aTGACATTACCGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCT‑GGTAAGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCtata                                                           >  2:517103/1‑139 (MQ=255)                                                               tGACATTACCGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCT‑GGTAAGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATaa                                                          >  2:172540/1‑139 (MQ=255)                                                                gACATTACCGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCT‑GGTAAGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAAt                                                         <  1:172540/139‑1 (MQ=255)                                                                 aCATTACCGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCT‑GGTAAGTATAACGTTGGCGTGAGCATCTTCACGCCGACGTGCTGTTACTTGCCGGAAAACg                                                                   <  2:906713/128‑1 (MQ=255)                                                                 aCATTACCGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCT‑GGTAAGTATAACGTTGGCGTGAGCATCTTCACGCCGACGTGCTGTTACTTGCCGGAAAACg                                                                   >  1:906713/1‑128 (MQ=255)                                                                     tACCGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCT‑GGTAAGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATaa                                                          <  1:950096/133‑1 (MQ=255)                                                                     tACCGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCT‑GGTAAGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATaa                                                          >  2:950096/1‑133 (MQ=255)                                                                        cGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCT‑GGTAAGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGa                                                     >  2:230852/1‑135 (MQ=255)                                                                        cGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCT‑GGTAAGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGa                                                     <  1:230852/135‑1 (MQ=255)                                                                                  aGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGGTAAGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGAGTa                                                  >  1:919041/1‑129 (MQ=255)                                                                                  aGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGGTAAGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGAGTa                                                  <  2:919041/129‑1 (MQ=255)                                                                                       aGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCT‑GGTAAGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGAGTAATTCATTCTTTATTTc                                  >  2:696472/1‑139 (MQ=255)                                                                                                  tGCGCGGCGAAAACCATTCAGTACCACGGTGCCT‑GGTAAGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGAGTAATTCATTCTTTATTTCAGGGTCGATTa                       <  2:401984/139‑1 (MQ=255)                                                                                                                       tACCACGGTGCCT‑GGTAAGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGAGTAattca                                             <  2:744268/96‑1 (MQ=255)                                                                                                                       tACCACGGTGCCT‑GGTAAGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGAGTAattca                                             >  1:744268/1‑96 (MQ=255)                                                                                                                       tACCACGGTGCCT‑GGTAAGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGAGTAATTCATTCTTTATTTCAGGGTCGATTATGTCAGCTACGGACCGCTAct  <  1:337197/139‑1 (MQ=255)                                                                                                                               tGCCT‑GGTAAGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGAGTAATTCATTCTTTATTTCAGGGTCGATTATGTCAGCTACg            <  2:14931/121‑1 (MQ=255)                                                                                                                               tGCCT‑GGTAAGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGAGTAATTCATTCTTTATTTCAGGGTCGATTATGTCAGCTACg            >  1:14931/1‑121 (MQ=255)                                                                                                                                         |                                                                                                                          CGACACCATGGGTGCCGGAGATTCGTTCATTGCCGGATTCCTTTGCGGCTGGTCTGCGGGGATGACATTACCGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCT‑GGTAGGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGAGTAATTCATTCTTTATTTCAGGGTCGATTATGTCAGCTACGGACCGCTACT  >  NC_000913/3503817‑3504073

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑