Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,506,024 T→C intergenic (‑4/+8) yhfS ← / ← yhfT FNR‑regulated pyridoxal phosphate‑dependent aminotransferase family protein/inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,506,0240TC100.0% 110.5 / NA 32intergenic (‑4/+8)yhfS/yhfTFNR‑regulated pyridoxal phosphate‑dependent aminotransferase family protein/inner membrane protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (13/19);  total (13/19)

AATCACCGCCAGTAAGAAACTCGCTGCCGGGGAAATGGCGACAAATGCTATCCACCAGCGCAAACTGCTTTTGCTGCGCCTCAATAATCGTCAGGCTTTGCAGAGGAAACGTCTTCATAGGTGCCTTCCTTAAGCCGGAACGAACAGGCCAAGCCAGTAAAGAACATTCAGCAGAATACCGGTGATCATTACTGCCACGACCGGTGCCGCCATTTTCTGTACCGGACGGCCCAGGGATTCGTTGAGGAAGTAAA  >  NC_000913/3505903‑3506156
                                                                                                                         |                                                                                                                                    
aaTCACCGCCAGTAAGAAGCTCGCTGCCGGGGAAATGGCGACAAATGCTATCCACCAGCGCAAACTGCTTTTGCTGCGCCTCAATAATCGTCAGGCTTTGCAGAGGAAACGTCTTCATAGGCGCCTTCCTTAAg                                                                                                                          >  1:559867/1‑134 (MQ=255)
aaTCACCGCCAGTAAGAAACTCGCTGCCGGGGAAATGGCGACAAATGCTATCCACCAGCGCAAACTGCTTTTGCTGCGCCTCAATAATCGTCAGGCTTTGCAGAGGAAACGTCTTCATAGGCGCCTTCCTTAAg                                                                                                                          <  2:559867/134‑1 (MQ=255)
aaTCACCGCCAGTAAGAAACTCGCTGCCGGGGAAATGGCGACAAATGCTATCCACCAGCGCAAACTGCTTTTGCTGCGCCTCAATAATCGTCAGGCTTTGCAGAGGAAACGTCTTCATAGGCGCCTTCCTTAAGCCGga                                                                                                                     <  2:887107/139‑1 (MQ=255)
 aTCACCGCCAGTAAGAAACTCGCTGCCGGGGAAATGGCGACAAATGCTATCCACCAGCGCAAACTGCTTTTGCTGCGCCTCAATAATCGTCAGGCTTTGCAGAGGAAACGTCTTCATAGGCGCCTTCCTTAAGCCGgaa                                                                                                                    >  2:853810/1‑139 (MQ=255)
          aGTAAGAAACTCGCTGCCGGGGAAATGGCGACAAATGCTATCCACCAGCGCAAACTGCTTTTGCTGCGCCTCAATAATCGTCAGGCTTTGCAGAGGAAACGTCTTCATAGGCGCCTTCCTTAAGCCGGAACGAACAGGc                                                                                                           <  1:25686/139‑1 (MQ=255)
              aGAAACTCGCTGCCGGGGAAATGGCGACAAATGCTATCCACCAGCGCAAACTGCTTTTGCTGCGCCTCAATAATCGTCACGCTTTGCAGAGGAAACGTCTTCATAGGCGCCTTCCTTAAGCCGGAACGAACAGGCCAAg                                                                                                       >  1:612258/1‑139 (MQ=255)
                   cTCGCTGCCGGGGAAATGGCGACAAATGCTATCCACCAGCGCAAACTGCTTTTGCTGCGCCTCAATAATCGTCAGGCTTTGCAGAGGAAACGTCTTCATAGGCGCCTTCCTTAAGCCGGAACGAACAGGCCAAGCCAGt                                                                                                  <  1:246763/139‑1 (MQ=255)
                       cTGCCGGGGAAATGGCGACAAATGCTATCCACCAGCGCAAACTGCTTTTGCTGCGCCTCAATAATCGTCAGGCTTTGCAGAGGAAACGTCTTCATAGGCGCCTTCCTTAAGCCGGAACGAACAGGCCAAg                                                                                                       <  1:1007215/130‑1 (MQ=255)
                       cTGCCGGGGAAATGGCGACAAATGCTATCCACCAGCGCAAACTGCTTTTGCTGCGCCTCAATAATCGTCAGGCTTTGCAGAGGAAACGTCTTCATAGGCGCCTTCCTTAAGCCGGAACGAACAGGCCAAg                                                                                                       >  2:1007215/1‑130 (MQ=255)
                        tGCCGGGGAAATGGCGACAAATGCTATCCACCAGCGCAAACTGCTTTTGCTGCGCCTCAATAATCGTCAGGCTTTGCAGAGGAAACGTCTTCATAGGCGCCTTCCTTAAGCCGGAACGAACAGGCCAAGCCAGTAAAGa                                                                                             <  1:146517/139‑1 (MQ=255)
                                         cAAATGCTATCCACCAGCTCAAACTGCTTTTGCTGCGCCTCAATAATCGTCAGGCTTTGCAGAGGAAACGTCTTCATAGGCGCCTTCCTTAAGCCGGAACGAACAGGCCAAGCCAGTAAAGAACATTCAGCAGAATAcc                                                                            <  2:632724/139‑1 (MQ=255)
                                          aaaTGCTATCCACCAGCGCAAACTGCTTTTGCTGCGCCTCAATAATCGTCAGGCTTTGCAGAGGAAACGTCTTCATAGGCGCCTTCCTTAAGCCGGAACGAACAGGCCAAGCCAGTAAAGAACATTCAGCAGAATACCg                                                                           <  2:612258/139‑1 (MQ=255)
                                                tATCCACCAGCGCAAACTGCTTTTGCTGCGCCTCAATAATCGTCAGGCTTTGCAGAGGAAACGTCTTCATAGGCGCCTTCCTTAAGCCGGAACGAACAGGCCAAGCCAGTAAAGAACATTCAGCAGAATACCGGTGATc                                                                     >  2:767514/1‑139 (MQ=255)
                                                      ccaGCGCAAACTGCTTTTGCTGCGCCTCAATAATCGTCAGGCTTTGCAGAGGAAACGTCTTCATAGGCGCCTTCCTTAAGCCGGAACGAACAGGCCAAGCCAGTAAAGAACATTCAGCAGAATACCGGTGATCATTACt                                                               >  1:528132/1‑139 (MQ=255)
                                                       caGCGCAAACTGCTTTTGCTGCGCCTCAATAATCGTCAGGCTTTGCAGAGGAAACGTCTTCATAGGCGCCTTCCTTAAGCCGgaacgaa                                                                                                                <  1:4852/89‑1 (MQ=255)
                                                       caGCGCAAACTGCTTTTGCTGCGCCTCAATAATCGTCAGGCTTTGCAGAGGAAACGTCTTCATAGGCGCCTTCCTTAAGCCGgaacgaa                                                                                                                >  2:4852/1‑89 (MQ=255)
                                                          cgcAAACTGCTTTTGCTGCGCCTCAATAATCGTCAGGCTTTGCAGAGGAAACGTCTTCATAGGCGcc                                                                                                                                   <  1:540604/67‑1 (MQ=255)
                                                          cgcAAACTGCTTTTGCTGCGCCTCAATAATCGTCAGGCTTTGCAGAGGAAACGTCTTCATAGGCGcc                                                                                                                                   >  2:540604/1‑67 (MQ=255)
                                                            cAAACTGCTTTTGCTGCGCCTCAATAATCGTCAGGCTTTGCAGAGGAAACGTCTTCATAGGCGCCTTCCTTAAGCCGGAACGAACAGGCCAAGCCAGTAAAGAACATTCAGCAGAATACCGGTGATCATTACTGCCACg                                                         >  2:870423/1‑139 (MQ=255)
                                                                    ttttGCTGCGCCTCAATAATCGTCAGGCTTTGCAGAGGAAACGTCTTCATAGGCGCCTTCCTTAAGCCGGAACGAACAGGCCAAGCCAGTAAAGAACATTCAGCAGAATACCGGTGATCATTACTGCCACGACCGGTgc                                                 <  2:528132/139‑1 (MQ=255)
                                                                      ttGCTGCGCCTCAATAATCGTCAGGCTTTGCAGAGGAAACGTCTTCATAGGCGCCTTCCTTAAGCCGGAACGAACAGGCCAAGCCAGTAAAGAACAATCAGCAGAATACCGGTGATCATTACTGCCACGACCGGTgccg                                               <  2:319209/139‑1 (MQ=255)
                                                                                      atCGTCAGGCTTTGCAGAGGAAACGTCTTCATAGGCGCCTTCCTTAAGCCGGAACGAACAGGCCAAGCCAGTAAAGAACATTCAGCAGAATACCGGTGATCATTACTGCCACGACCGGTGCCGCCATTTTCTGTACCgg                               >  2:914489/1‑139 (MQ=255)
                                                                                       tCGTCAGGCTTTGCAGAGGAAACGTCTTCATAGGCGCCTTCCTTAAGCCGGAACGAACAGGCCAAGCCAGTAAAGAACATTCAGCAGAATACCGGTGATCATTACTGCCACGACCGGTGCCGCCATTTTCTGTACCGGa                              <  1:767514/139‑1 (MQ=255)
                                                                                       tCGTCAGGCTTTGCAGAGGAAACGTCTTCATAGGCGCCTTCCTTAAGCCGGAACGAACAGGCCAAGCCAGTAAAGAACATTCAGCAGAATACCGGCGATCATTACTGCCACGACCGGTGCCGCCATTTTCTGTACCGGa                              <  1:853810/139‑1 (MQ=255)
                                                                                            aGGCTTTGCAGAGGAAACGTCTTCATAGGCGCCTTCCTTAAGCCGGAACGAACAGGCCAAGCCAGTAAAGAACATTCAGCAGAATACCGGTGATCATTACTGCCACGACCGGTGCCGCCATTTTCTGTACCGGACGGcc                         <  2:558973/139‑1 (MQ=255)
                                                                                            aGGCTTTGCAGAGGAAACGTCTTCATAGGCGCCTTCCTTAAGCCGGAACGAACAGGCCAAGCCAGTAAAGAACATTCAGCAGAATACCGGTGATCATTACTGCCACGACCGGTGCCGCCATTTTCTGTACCGGACGGcc                         <  1:565531/139‑1 (MQ=255)
                                                                                                        ggAAACGTCTTCATAGGCGCCTTCCTTAAGCCGGAACGAACAGGCCAAGCCAGTAAAGAACATTcag                                                                                     >  2:112548/1‑67 (MQ=255)
                                                                                                        ggAAACGTCTTCATAGGCGCCTTCCTTAAGCCGGAACGAACAGGCCAAGCCAGTAAAGAACATTcag                                                                                     <  1:112548/67‑1 (MQ=255)
                                                                                                         gAAACGTCTTCATAGGCGCCTTCCTTAAGCCGGAACGAACAGGCCAAGCCAGTAAAGAACATTCAGCAGAATACCGGTGATCATTACTGCCACGACCGGTGCCGCCATTTTCTGTACCGGACGGCCCAGGGATTCGTTg            <  1:528883/139‑1 (MQ=255)
                                                                                                                 ttCATAGGCGCCTTCCTTAAGCCGGAACGAACAGGCCAAGCCAGTAAAGAACATTCAGCAGAATACCGGTGATCATTACTGCCACGACCGGTGCCGCCATTTTCTGTACCGGa                              <  2:391229/113‑1 (MQ=255)
                                                                                                                 ttCATAGGCGCCTTCCTTAAGCCGGAACGAACAGGCCAAGCCAGTAAAGAACATTCAGCAGAATACCGGTGATCATTACTGCCACGACCGGTGCCGCCATTTTCTGTACCGGa                              >  1:391229/1‑113 (MQ=255)
                                                                                                                   cATAGGCGCCTTCCTTAAGCCGGAACGAACAGGCCAAGCCAGTAAAGAACATTCAGCAGAATACCGGTGATCATTACTGCCACGACCGGTGCCGCCATTTTCTGTACCGGACGGCCCAGGGATTCGTTGAGGAAGTaaa  >  1:956700/1‑139 (MQ=255)
                                                                                                                         |                                                                                                                                    
AATCACCGCCAGTAAGAAACTCGCTGCCGGGGAAATGGCGACAAATGCTATCCACCAGCGCAAACTGCTTTTGCTGCGCCTCAATAATCGTCAGGCTTTGCAGAGGAAACGTCTTCATAGGTGCCTTCCTTAAGCCGGAACGAACAGGCCAAGCCAGTAAAGAACATTCAGCAGAATACCGGTGATCATTACTGCCACGACCGGTGCCGCCATTTTCTGTACCGGACGGCCCAGGGATTCGTTGAGGAAGTAAA  >  NC_000913/3505903‑3506156

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: