Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	340,089	G→A	intergenic (+346/‑76)	yahG → / → yahI	DUF1116 family protein/carbamate kinase‑like protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	340,089	0	G	A	100.0%	156.5 / NA	49	intergenic (+346/‑76)	yahG/yahI	DUF1116 family protein/carbamate kinase‑like protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (24/25);  total (24/25)

GACGCCTTATCCGGCCTACGAGTGGCGCGAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATAACAACATGAAAGAGCTTGTGGTCGTTGCCATTGGTGGCAACAGCATTATCAAAGATAACGCC  >  NC_000913/339958‑340221                                                                                                                                    |                                                                                                                                     gACGCCTTATCCGGCCTACGAGTGGCGCGAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAAGCCTGAt                                                                                                                               >  2:862047/1‑139 (MQ=255)      cTTATCCGGCCTACGAGTGGCGCGAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAAGCCTGATAAGCg                                                                                                                          >  1:136147/1‑139 (MQ=255)        tATCCGGCCTACGAGTGGCGCGAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAAGCCTGATAAGCGTa                                                                                                                        <  1:862047/139‑1 (MQ=255)                  aCGAGTGGCGCGAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAAGCCTGATAAGCGTAGCGCATCAgg                                                                                                              <  2:299790/139‑1 (MQ=255)                  aCGAGTGGCGCGAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAAGCCTGATAAGCGTAGCGCATCAgg                                                                                                              <  2:136147/139‑1 (MQ=255)                    gAGTGGCGCGAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAAGCCTGATAAGCGTAGCGCATCAGGCa                                                                                                            >  2:488958/1‑139 (MQ=255)                         gcgcGAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAAGCCTGATAAGCGTAGCGCATCAGGCAGTCTg                                                                                                       >  2:950506/1‑139 (MQ=255)                                    tGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAAGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCAt                                                                                            >  2:569034/1‑139 (MQ=255)                                           cTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAAGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCa                                                                                     <  1:781964/139‑1 (MQ=255)                                              aTAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAAGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGt                                                                                                   <  2:91073/122‑1 (MQ=255)                                              aTAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAAGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGt                                                                                                   >  1:91073/1‑122 (MQ=255)                                              aTAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAAGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCa                                                                                  >  1:799030/1‑139 (MQ=255)                                                aaGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAAGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCAcc                                                                                <  2:872327/139‑1 (MQ=255)                                                   cGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAAGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCctc                                                                             >  2:73288/1‑139 (MQ=255)                                                   cGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAAGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCct                                                                              >  1:946514/1‑138 (MQ=255)                                                   cGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAAGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCct                                                                              <  2:946514/138‑1 (MQ=255)                                                       gcgcATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAAGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTa                                                                         <  1:488958/139‑1 (MQ=255)                                                        cgcATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAAGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCg                                                                                                    >  2:384294/1‑111 (MQ=255)                                                        cgcATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAAGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCg                                                                                                    <  1:384294/111‑1 (MQ=255)                                                        cgcATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAAGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAg                                                                        >  2:636904/1‑139 (MQ=255)                                                           aTCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAAGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCgg                                                                     >  1:656820/1‑139 (MQ=255)                                                            tCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCATATCCGGCCTACGAGTGGCGCGAGAATTGTAAGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGa                                                                    <  1:636904/139‑1 (MQ=255)                                                                     tGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAAGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATaacaac                                                           <  2:785731/139‑1 (MQ=255)                                                                     tGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAAGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATaacaac                                                           >  1:785731/1‑139 (MQ=255)                                                                          ccATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAAGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATAACAACATGaa                                                      <  2:799030/139‑1 (MQ=255)                                                                                      gcggcgTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAAGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATAACAACATGAAAGAGCTTGTGGt                                          <  1:785495/139‑1 (MQ=255)                                                                                          cgTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAAGCCTGATAAGCGTa                                                                                                                        >  1:737513/1‑57 (MQ=255)                                                                                          cgTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAAGCCTGATAAGCGTa                                                                                                                        <  2:737513/57‑1 (MQ=255)                                                                                                      aTCCGGCCTACGAGTGGCGCGAGAATTGTAAGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATAACAACATGAAaga                                                   >  2:458515/1‑114 (MQ=255)                                                                                                      aTCCGGCCTACGAGTGGCGCGAGAATTGTAAGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATAACAACATGAAaga                                                   <  1:458515/114‑1 (MQ=255)                                                                                                        ccGGCCTACGAGTGGCGCGAGAATTGTAAGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATAACAACATGAAAGAGCTTGTGGTCGTTGCCATTGGTGGCaa                        <  1:950506/139‑1 (MQ=255)                                                                                                           gCCTACGAGTGGCGCGAGAATTGTAAGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATAACAACATGAAAGAGCTTGTGGTCGTTGCCATTGGTGGCAACAg                     >  2:407071/1‑139 (MQ=255)                                                                                                             cTACGAGTGGCTCGAGAATTGTAAGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATAACAACATGAAAGAGCTTGTGGTCGTTGCCATTGGTGCCAACAGCa                   <  2:656820/139‑1 (MQ=255)                                                                                                             cTACGAGTGGCGCGAGAATTGTAAGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCg                                                                                                    <  2:619198/58‑1 (MQ=255)                                                                                                             cTACGAGTGGCGCGAGAATTGTAAGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCg                                                                                                    >  1:619198/1‑58 (MQ=255)                                                                                                             cTACGAGTGGCGCGAGAATTGTAAGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATAACAACATGAAAGAGCTTGTGGTCGTTGCCATTGGTGGCAACAGCa                   <  1:658878/139‑1 (MQ=255)                                                                                                               aCGAGTGGCGCGAGAATTGTAAGCCTGATAAGCGTAGCGCATCAgg                                                                                                              <  2:359335/46‑1 (MQ=255)                                                                                                               aCGAGTGGCGCGAGAATTGTAAGCCTGATAAGCGTAGCGCATCAgg                                                                                                              >  1:359335/1‑46 (MQ=255)                                                                                                                cGAGTGGCGCGAGAATTGTAAGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATaa                                                                                          >  2:924653/1‑65 (MQ=255)                                                                                                                cGAGTGGCGCGAGAATTGTAAGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATaa                                                                                          <  1:924653/65‑1 (MQ=255)                                                                                                                    tGGCGCGAGAATTGTAAGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATAACAACATGAAAGAGCTTGTGGTCGTTGCCATTGGTGGCAACAGCATTATCaa            >  2:585030/1‑139 (MQ=255)                                                                                                                      gcgcGAGAATTGTAAGCCTGATAAGCGTAGCGCATCAGGCAGTCTg                                                                                                       >  2:423701/1‑46 (MQ=255)                                                                                                                      gcgcGAGAATTGTAAGCCTGATAAGCGTAGCGCATCAGGCAGTCTg                                                                                                       <  1:423701/46‑1 (MQ=255)                                                                                                                      gcgcGAGAATTGTAAGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAg                                                                        <  1:132704/77‑1 (MQ=255)                                                                                                                      gcgcGAGAATTGTAAGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAg                                                                        >  2:132704/1‑77 (MQ=255)                                                                                                                      gcgcGAGAATTGTAAGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATAACAACATGAAAGAGCTTGTGGTCGTTGCCATTGGTGGCAACAGCa                   >  2:506398/1‑130 (MQ=255)                                                                                                                      gcgcGAGAATTGTAAGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATAACAACATGAAAGAGCTTGTGGTCGTTGCCATTGGTGGCAACAGCa                   <  1:506398/130‑1 (MQ=255)                                                                                                                      gcgcGAGAATTGTAAGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATAACAACATGAAAGAGCTTGTGGTCGTTGCCATTGGTGGCAACAGCATTATCAAAg          >  1:160910/1‑139 (MQ=255)                                                                                                                              aTTGTAAGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATAACAACATGAAAGAGCTTGTGGTCGTTGCCATTGGTGGCAACAGCATTATCAAAGATAACgcc  <  1:804018/139‑1 (MQ=255)                                                                                                                                    |                                                                                                                                     GACGCCTTATCCGGCCTACGAGTGGCGCGAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATAACAACATGAAAGAGCTTGTGGTCGTTGCCATTGGTGGCAACAGCATTATCAAAGATAACGCC  >  NC_000913/339958‑340221

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑