Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,531,180 G→A A420A (GCC→GCT)  yhgE ← DUF4153 family putative inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,531,1800GA100.0% 181.0 / NA 56A420A (GCC→GCT) yhgEDUF4153 family putative inner membrane protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (27/29);  total (27/29)

AACCAGGGGCAATTAACACATTATCGGCTAATACTTTTTCCGATACCTGTTGCTGAAGATGTTGCTCTCCATCTAACGCCATCAGCAGATCCCGCGCGCGTTTCGGGTCTTTCATAAACCCGGCATCGCTTTTCAGCGACTCAAGCGCAGCACGTCCATAGCGACCGCTCTGCTCGAGCATGTAGATAGTTACCTGGTCTGACGTGTTTTTGCCGCTCTGATAACGCGCCATATGGCTGTTCACGCT  >  NC_000913/3531059‑3531305
                                                                                                                         |                                                                                                                             
aaCCAGGGGCAATTAACACATTATCGGCTAATACTTTTTCCGATACCTGTTGCTGAAGATGTTGCTCTCCATCTAACGCCATCAGCAGATCCCGCGCGCGTTTCGGGTCTTTCATAAACCCAGCa                                                                                                                            <  2:802938/125‑1 (MQ=255)
aaCCAGGGGCAATTAACACATTATCGGCTAATACTTTTTCCGATACCTGTTGCTGAAGATGTTGCTCTCCATCTAACGCCATCAGCAGATCCCGCGCGCGTTTCGGGTCTTTCATAAACCCAGCa                                                                                                                            >  1:802938/1‑125 (MQ=255)
  ccAGGGGCAATTAACACATTATCGGCTAATACTTTTTCCGATACCTGTTGCTGAAGATGTTGCTCTCCATCTAACGCCATCAGCAGATCCCGCGCGCGTTTCGGGTCTTTCATAAACCCAGCATCGCTTTTCAGCGACt                                                                                                            >  1:809443/1‑139 (MQ=255)
           aTTAACACATTATCGGCTAATACTTTTTCCGATACCTGTTGCTGAAGATGTTGCTCTCCATCTAACGCCATCAGCAGATCACGCGCGCGTTTCGGGTCTTTCATAAACCCAGCATCGCTTTTCAGCGACTCAAGCgcag                                                                                                   <  2:204189/139‑1 (MQ=255)
                    ttATCGGCTAATACTTTTTCCGATACCTGTTGCTGAAGATGTTGCTCTCCATCTAACGCCATCAGCAGATCCCGCGCGCGTTTCGGGTCTTTCATAAACCCAGCATCGCTTTTCAGCGACTCAAGCGCAGCACGTCCAt                                                                                          >  1:889325/1‑139 (MQ=255)
                       tCGGCTAATACTTTTTCCGATACCTGTTGCTGAAGATGTTGCTCTCCATCTAACGCCATCAGCAGATCCCGCGCGCGTTTCGGGTCTTTCATAAACCCAGCATCGCTTTTCAGCGACTCAAGCGCAGCACGTCCATAGc                                                                                       <  2:889325/139‑1 (MQ=255)
                       tCGGCTAATACTTTTTCCGATACCTGTTGCTGAAGATGTTGCTCTCCATCTAACGCCATCAGCAGATCCCGCGCACGTTTCGGGTCTTTCATAAACCCAGCATCGCTTTTCAGCGACTCAAGCGCAGCACGTCCATAGc                                                                                       >  1:61545/1‑139 (MQ=255)
                          gCTAATACTTTTTCCGATACCTGTTGCTGAAGATGTTGCTCTCCATCTAACGCCATCAGCAGATCCCGCGCGCGTTTCGGGTCTTTCATAAACCCAGCATCGCTTTTCAGCGACTCAAGCGCAGCACGTCCATAGCGAc                                                                                    <  1:523654/139‑1 (MQ=255)
                          gCTAATACTTTTTCCGATACCTGTTGCTGAAGATGTTGCTCTCCATCTAACGCCATCAGCAGATCCCGCGCGCGTTTCGGGTCTTTCATAAACCCAGCATCGCTTTTCAGCGACTCAAGCGCAGCACGTCCATAGCGAc                                                                                    >  2:458344/1‑139 (MQ=255)
                           cTAATACTTTTTCCGATACCTGTTGCTGAAGATGTTGCTCTCCATCTAACGCCATCAGCAGATCCCGCGCGCGTTTCGGGTCTTTCATAAACCCAGCATCGCTTTTCAGCGACTCAAGCGCAGCACGTCCATAGCGAcc                                                                                   >  2:520137/1‑139 (MQ=255)
                              aTACTTTTTCCGATACCTGTTGCTGAAGATGTTGCTCTCCATCTAACGCCATCAGCAGATCCCGCGCGCGTTTCGGGTCTTTCATAAACCCAGCATCGCTTTTCAGCGACTCAAGCGCAGCACGTCCATAGCGACCGct                                                                                >  1:647853/1‑139 (MQ=255)
                               tACTTTTTCCGATACCTGTTGCTGAAGATGTTGCTCTCCATCTAACGCCATCAGCAGATCCCGCGCGCGTTTCGGGTCTTTCATAAACCCAGCATCGCTTTTCAGCGACTCAAGCGCAGCACGTCCATAGCGACCGct                                                                                >  1:647342/1‑138 (MQ=255)
                               tACTTTTTCCGATACCTGTTGCTGAAGATGTTGCTCTCCATCTAACGCCATCAGCAGATCCCGCGCGCGTTTCGGGTCTTTCATAAACCCAGCATCGCTTTTCAGCGACTCAAGCGCAGCACGTCCATAGCGACCGct                                                                                <  2:647342/138‑1 (MQ=255)
                                   ttttCCGATACCTGTTGCTGAAGATGTTGCTCTCCATCTAACGCCATCAGCAGATCCCGCGCGCGTTTCGGGTCTTTCATAAACCCAGCATCGCTTTTCAGCGACTCAAGCGCAGCACGTCCATAGCGACCGCTCTGCt                                                                           <  2:809443/139‑1 (MQ=255)
                                     ttCCGATACCTGTTGCTGAAGATGTTGCTCTCCATCTAACGCCATCAGCAGATCCCGCGCGCGTTTCGGGTCTTTCATAAACCCAGCATCGCTTTTCAGCGACTCAAGCGCAGCACGTCCATAGCGACCGCTCTGCTCg                                                                         <  1:520137/139‑1 (MQ=255)
                                     ttCCGATACCTGTTGCTGAAGATGTTGCTCTCCATCTAACGCCATCAGCAGATCCCGCGCACGTTTCGGGTCTTTCATAAACCCAGCATCGCTTTTCAGCGACTCAAGCGCAGCACGTCCATAGCGACCGCTCTGCTCg                                                                         <  2:61545/139‑1 (MQ=255)
                                        cGATACCTGTTGCTGAAGATGTTGCTCTCCATCTAACGCCATCAGCAGATCCCGCGCGCGTTTCGGGTCTTTCATAAACCCAGCATCGCTTTTCAGCGACTCAAGCGCAGCACGTCCATAGCGACCGCTCTGCTCGAGc                                                                      <  1:458344/139‑1 (MQ=255)
                                          aTACCTGTTGCTGAAGATGTTGCTCTCCATCTAACGCCATCAGCAGATCCCGCGCGCGTTTCGGGTCTTTCATAAACCCAGCATCGCTTTTCAGCGACTCAAGCgcag                                                                                                   >  1:944881/1‑108 (MQ=255)
                                          aTACCTGTTGCTGAAGATGTTGCTCTCCATCTAACGCCATCAGCAGATCCCGCGCGCGTTTCGGGTCTTTCATAAACCCAGCATCGCTTTTCAGCGACTCAAGCgcag                                                                                                   <  2:944881/108‑1 (MQ=255)
                                          aTACCTGTTGCTGAAGATGTTGCTCTCCATCTAACGCCATCAGCAGATCCCGCGCGCGTTTCGGGTCTTTCATAAACCCAGCATCGCTTTTCAGCGACTCAAGCGCAGCACGTCCATAGCGACCGCTCTGCTCGAGCAt                                                                    >  1:154775/1‑139 (MQ=255)
                                            aCCTGTTGCTGAAGATGTTGCTCTCCATCTAACGCCATCAGCAGATCCCGCGCGCGTTTCGGGTCTTTCATAAGCCCAGCATCGCTTTTCAGCGACTCAAGCGCAGCACGTCCATAGCGACCGCTCTGCTCGAGCATGt                                                                  <  2:183824/139‑1 (MQ=255)
                                                gTTGCTGAAGATGTTGCTCTCCATCTAACGCCATCAGCAGATCCCGCGCGCGTTTCGGGTCTTTCATAAACCCAGCATCGCTTTTCAGc                                                                                                                >  1:502646/1‑89 (MQ=255)
                                                gTTGCTGAAGATGTTGCTCTCCATCTAACGCCATCAGCAGATCCCGCGCGCGTTTCGGGTCTTTCATAAACCCAGCATCGCTTTTCAGc                                                                                                                <  2:502646/89‑1 (MQ=255)
                                                gTTGCTGAAGATGTTGCTCTCCATCTAACGCCATCAGCAGATCCCGCGCGCGTTTCGGGTCTTTCATAAACCCAGCATCGCTTTTCAGCGACTCAAGCGCAGCACGTCCATAGCg                                                                                      <  1:705909/115‑1 (MQ=255)
                                                gTTGCTGAAGATGTTGCTCTCCATCTAACGCCATCAGCAGATCCCGCGCGCGTTTCGGGTCTTTCATAAACCCAGCATCGCTTTTCAGCGACTCAAGCGCAGCACGTCCATAGCg                                                                                      >  2:705909/1‑115 (MQ=255)
                                                        aGATGTTGCTCTCCATCTAACGCCATCAGCAGATCCCGCGCGCGTTTCGGGTCTTTCATAAACCCAGCATCGCTTTTCAGCGACTCAAGCgcag                                                                                                   <  2:487857/94‑1 (MQ=255)
                                                        aGATGTTGCTCTCCATCTAACGCCATCAGCAGATCCCGCGCGCGTTTCGGGTCTTTCATAAACCCAGCATCGCTTTTCAGCGACTCAAGCgcag                                                                                                   >  1:487857/1‑94 (MQ=255)
                                                            gTTGCTCTCCATCTAACGCCATCAGCAGATCCCGCGCGCGTTTCGGGTCTTTCATAAACCCAGCATCGCTTTTCAGCGACTCAAGCGCAGCACGTCCATAGCGACCGCTCTGCTCGAGCATGTAGATAGTTACCTGGTc                                                  >  2:456345/1‑139 (MQ=255)
                                                                 tctcCATCTAACGCCATCAGCAGATCCCGCGCGCGTTTCGGGTCTTTCATAAACCCAGCATCGCTTTTCAGCGAc                                                                                                             <  1:909726/75‑1 (MQ=255)
                                                                 tctcCATCTAACGCCATCAGCAGATCCCGCGCGCGTTTCGGGTCTTTCATAAACCCAGCATCGCTTTTCAGCGAc                                                                                                             >  2:909726/1‑75 (MQ=255)
                                                                        cTAACGCCATCAGCAGATCCCGCGCGCGTTTCGGGTCTTTCATAAACCCAGCATCGCTTTTCAGCGACTCAAGCGCAGCACGTCCATAGCGACCGCTCTGCTCGAGCATGTAGATAGTTACCTGGTCTGACGTGttttt                                      >  1:122526/1‑139 (MQ=255)
                                                                            cGCCATCAGCAGATCCCGCGCGCGTTTCGGGTCTTTCATAAACCCAGCATCGCTTTTCAGCGACTCAAgc                                                                                                       <  1:695998/70‑1 (MQ=255)
                                                                            cGCCATCAGCAGATCCCGCGCGCGTTTCGGGTCTTTCATAAACCCAGCATCGCTTTTCAGCGACTCAAgc                                                                                                       >  2:695998/1‑70 (MQ=255)
                                                                               cATCAGCAGATCCCGCGCGCGTTTCGGGTCTTTCATAAACCCAGCATCGCTTTTCAGCGACTCAAGCGCAGCACGTCCATAGCGACCg                                                                                  >  2:306475/1‑88 (MQ=255)
                                                                               cATCAGCAGATCCCGCGCGCGTTTCGGGTCTTTCATAAACCCAGCATCGCTTTTCAGCGACTCAAGCGCAGCACGTCCATAGCGACCg                                                                                  <  1:306475/88‑1 (MQ=255)
                                                                               cATCAGCAGATCCCGCGCGCGTTTCGGGTCTTTCATAAACCCAGCATCGCTTTTCAGCGACTCAAGCGCAGCACGTCCATAGCGACCGCTCTGCTCGAGCATGTAGATAGTTACCTGGTCTGACGTGTTTTTGCCGctc                               >  1:128097/1‑139 (MQ=255)
                                                                                 tCAGCAGATCCCGCGCGCGTTTCGGGTCTTTCATAAACCCAGCATCGCTTTTCAGCGACTCAAGCGCAGCACGTCCATAGCg                                                                                      <  2:393651/82‑1 (MQ=255)
                                                                                 tCAGCAGATCCCGCGCGCGTTTCGGGTCTTTCATAAACCCAGCATCGCTTTTCAGCGACTCAAGCGCAGCACGTCCATAGCg                                                                                      >  1:393651/1‑82 (MQ=255)
                                                                                 tCAGCAGATCCCGCGCGCGTTTCGGGTCTTTCATAAACCCAGCATCGCTTTTCAGCGACTCAAGCGCAGCACGTCCATAGCGACCGCTCTGCTCGAGCATGTAGATAGTTACCTGGTCTGACGTGTTTTTGCCGCTCTg                             >  2:246794/1‑139 (MQ=255)
                                                                                       gATCCCGTGCGTATTTCGGGTCTTTCATAAACCCAGCATCGCTTTTCAGCGACTCAAGCGCAGCACGTCCATAGCGACCGCTCTGCTCGAGCATGTAGATAGTTACCTGTTCTGACGTGTTTTTGCCGCTCTGATAAcg                       <  1:138921/139‑1 (MQ=255)
                                                                                       gATCCCGCGCGCGTTTCGGGTCTTTCATAAACCCAGCATCGCTTTTCAGCGACTCAAGCGCAGCACGTCCATAGCGACCGCTCTGCTCGAGCATGTAGATAGTTACCTGGTCTGACGTGTTTTTGCCGCTCTGATAAcg                       <  2:647853/139‑1 (MQ=255)
                                                                                             gcgcgcgTTTCGGGTCTTTCATAAACCCAGCATCGCTTTTCAGCGACTCAAGCGCAGCACGTCCATAGCGACCGCTCTGCTCGAGCATGTAGATAGTTACCTGGTCTGACGTGTTTTTGCCGCTCTGATAAcg                       >  2:174462/1‑133 (MQ=255)
                                                                                             gcgcgcgTTTCGGGTCTTTCATAAACCCAGCATCGCTTTTCAGCGACTCAAGCGCAGCACGTCCATAGCGACCGCTCTGCTCGAGCATGTAGATAGTTACCTGGTCTGACGTGTTTTTGCCGCTCTGATAAcg                       <  1:174462/133‑1 (MQ=255)
                                                                                             gcgcgcgTTTCGGGTCTTTCATAAACCCAGCATCGCTTTTCAGCGACTCAAGCGCAGCACGTCCATAGCGACCGCTCTGCTCGAGCATGTAGATAGTTACCTGGTCTGACGTGTTTTTGCCGCTCTGATAACGCGCCat                 >  2:486200/1‑139 (MQ=255)
                                                                                              cgcgcgTTTCGGGTCTTTCATAAACCCAGCATCGCTTTTCAGCGACTCAAGCGCAGCACGTCCATAGCGACCGCTCTGCTCGAGCATGTAGATAGTTACCTGGTCTGACGTGTTTTTGc                                    >  1:672395/1‑119 (MQ=255)
                                                                                              cgcgcgTTTCGGGTCTTTCATAAACCCAGCATCGCTTTTCAGCGACTCAAGCGCAGCACGTCCATAGCGACCGCTCTGCTCGAGCATGTAGATAGTTACCTGGTCTGACGTGTTTTTGc                                    <  2:672395/119‑1 (MQ=255)
                                                                                                 gcgTTTCGGGTCTTTCATAAACCCAGCATCGCTTTTCAGCGACTCAAGCGCAGCACGTCCATAGCGACCGCTCTGCTCGAGCATGTAGATAGTTACCTGGTCTGACGTGTTTTTGCCGCTCTGATAACGCGCCATATgg             <  1:237807/139‑1 (MQ=255)
                                                                                                  cgTTTCGGGTCTTTCATAAACCCAGCATCGCTTTTCAGCGACTCAAGCGCAGCACGTCCATAGCGACCGCTCTGCTCGAGCATGTAGATAGTTACCTGGTCTGACGTGTTTTTGCCGCTCTGATAACGCGCCATATTGc            >  2:751213/1‑139 (MQ=255)
                                                                                                      tCGGGTCTTTCATAAACCCAGCATCGCTTTTCAGCGACTCAAGCGCAGCACGTCCATAGCGACCGCTCTGCTCGAGCATGTAGATAGTTACCTGGTCTGACGTGTTTTTGCCGCTCTGATAACGCGCCATATGGCTGtt        <  1:456345/139‑1 (MQ=255)
                                                                                                        gggTCTTTCATAAACCCAGCATCGCTTTTCAGCGACTCAAGCGCAGCACGTCCATAGCGACCGCTCTGCTCGAGCATGTAGATAGTTACCTGGTCTGACGTGTTTTTGCCGCTCTGATAACGCGCCATATGGCTGTTCa      <  1:246794/139‑1 (MQ=255)
                                                                                                          gTCTTTCATAAACCCAGCATCGCTTTTCAGCGACTCAAGCGCAGCACGTCCATAGCGACCGCTCTGCTCGAGCATGTAGATAGTTACCTGGTCTGACGTGTTTTTGCCGCTCTGATAACGCGCCATATGGCTGTTCACg    <  2:128097/139‑1 (MQ=255)
                                                                                                            cTTTCATAAACCCAGCATCGCTTTTCAGCGACTCAAGCGCAGCACGTCCATAGCGACCGCTCTGCTCGAGCATGTAGATAGTTACCTGGTCTGACGTGTTTTTGCCGCTCTGATAACGCGCCATATGGCTGTTCACGCt  <  2:122526/139‑1 (MQ=255)
                                                                                                             tttCATAAACCCAGCATCGCTTTTCAGCGACTCAAGCGCAGCACGTCCATAGCGa                                                                                     >  1:475769/1‑55 (MQ=255)
                                                                                                             tttCATAAACCCAGCATCGCTTTTCAGCGACTCAAGCGCAGCACGTCCATAGCGa                                                                                     <  2:475769/55‑1 (MQ=255)
                                                                                                               tCATAAACCCAGCATCGCTTTTCAGCGACTCAAGCGCAGCACg                                                                                               >  2:109641/1‑43 (MQ=255)
                                                                                                               tCATAAACCCAGCATCGCTTTTCAGCGACTCAAGCGCAGCACg                                                                                               <  1:109641/43‑1 (MQ=255)
                                                                                                                         |                                                                                                                             
AACCAGGGGCAATTAACACATTATCGGCTAATACTTTTTCCGATACCTGTTGCTGAAGATGTTGCTCTCCATCTAACGCCATCAGCAGATCCCGCGCGCGTTTCGGGTCTTTCATAAACCCGGCATCGCTTTTCAGCGACTCAAGCGCAGCACGTCCATAGCGACCGCTCTGCTCGAGCATGTAGATAGTTACCTGGTCTGACGTGTTTTTGCCGCTCTGATAACGCGCCATATGGCTGTTCACGCT  >  NC_000913/3531059‑3531305

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑