Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,545,565	G→A	*228* (TAG→TAA)	gntX →	DNA catabolic protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,545,565	0	G	A	100.0%	187.8 / NA	58	*228* (TAG→TAA)	gntX	DNA catabolic protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (28/30);  total (28/30)

CCCGTGCAAGGTCGCCATATGGTGATTGTGGATGATGTCGTTACCACCGGAAGTACCGTCGCAGAGATTGCGCAGTTGCTTTTACGCAATGGTGCGGCGGCTGTCCAGGTCTGGTGCCTTTGTCGAACCTTGTAGAGCCTCGATGATGGGCGTATTATAACCAACTAAAATAGTCAACTATTAGGCCATTACTATGATCCGTATTTCCGATGCTGCACAAGCGCACTTTGCCAAACTGCTGGCAAATCAGGA  >  NC_000913/3545431‑3545682                                                                                                                                       |                                                                                                                      cccGTGCAAGGTCGCCATATGGTGATTGTGGATGATGTCGTTACCACCGGAAGTACCGTCGCAGAGATTGCGCAGTTGCTTTTACGCAATGGTGCGGCGGCTGTCCAGGTCTGGTGCCTTTGTCGAACCTTGTAAAGcc                                                                                                                   >  2:77288/1‑139 (MQ=255) cccGTGCAAGGTCGCCATATGGTGATTGTGGATGATGTCGTTACCACCGGAAGTACCGTCGCAGAGATTGCGCAGTTGCTTTTACGCAATGGTGCGGCGGCTGTCCAGGTCTGGTGCCTTTGTCGAACCTTGTAAAGcc                                                                                                                   >  1:656461/1‑139 (MQ=255)               ccATATGGTGATTGTGGATGATGTCGTTACCACCGGAAGTACCGTCGCAGAGATTGCGCAGTTGCTTTTACGCAATGGTGCGGCGGCTGTCCAGGTCTGGTGCCTTTGTCGAACCTTGTAAAGCCTCGATGATGGGc                                                                                                       <  1:839289/137‑1 (MQ=255)               ccATATGGTGATTGTGGATGATGTCGTTACCACCGGAAGTACCGTCGCAGAGATTGCGCAGTTGCTTTTACGCAATGGTGCGGCGGCTGTCCAGGTCTGGTGCCTTTGTCGAACCTTGTAAAGCCTCGATGATGGGc                                                                                                       >  2:839289/1‑137 (MQ=255)                     ggTGATTGTGGATGATGTCGTTACCACCGGAAGTACCGTCGCAGAGATTGCGCAGTTGCTTTTACGCAATGGTGCGGCGGCTGTCCAGGTCTGGTGCCTTTGTCGAACCTTGTAAAGCCTCGATGATGGGCGTATtata                                                                                               >  1:478622/1‑139 (MQ=255)                      gTGATTGTGGATGATGTCGTTACCACCGGAAGTACCGTCGCAGAGATTGCGCAGTTGCTTTTACGCAATGGTGCGGCGGCTGTCCAGGTCTGGTGCCTTTGTCGAACCTTGTAAAGCCTCGATGATGGGCGTATTATaa                                                                                              <  1:151437/139‑1 (MQ=255)                      gTGATTGTGGATGATGTCGTTACCACCGGAAGTACCGTCGCAGAGATTGCGCAGTTGCTTTTACGCAATGGTGCGGCGGCTGTCCAGGTCTGGTGCCTTTGTCGAACCTTGTAAAGCCTCGATGATGGGCGTATTATaa                                                                                              <  1:703008/139‑1 (MQ=255)                         aTTGTGGATGATGTCGTTACCACCGGAAGTACCGTCGCAGAGATTGCGCAGTTGCTTTTACGCAATGGTGCGGCGGCTGTCCAGGTCTGGTGCCTTTGTCGAACCTTGTAAAGCCTCgatga                                                                                                            <  2:676413/122‑1 (MQ=255)                         aTTGTGGATGATGTCGTTACCACCGGAAGTACCGTCGCAGAGATTGCGCAGTTGCTTTTACGCAATGGTGCGGCGGCTGTCCAGGTCTGGTGCCTTTGTCGAACCTTGTAAAGCCTCgatga                                                                                                            >  1:676413/1‑122 (MQ=255)                          ttGTGGATGATGTCGTTACCACCGGAAGTACCGTCGCAGAGATTGCGCAGTTGCTTTTACGCAATGGTGCGGCGGCTGTCCAGGTCTGGTGCCTTTGTCGAACCTTGTAAAGCCTCGATGATGGGCGTATTATAACCaa                                                                                          >  2:204663/1‑139 (MQ=255)                            gtgGATGATGTCGTTACCACAGGAAGTACCGTCGCAGAGATTGCGCAGTTGCTTTTACGCAATGGTGCGGCGGCTGTCCAGGTCTGGTGCCTTTGTCGAACCTTGTAAAGCCTCGATGATGGGCGTATTATAACCAACt                                                                                        >  2:86106/1‑139 (MQ=255)                               gatgatGTCGTTACCACCGGAAGTACCGTCGCAGAGATTGCGCAGTTGCTTTTACGCAATGGTGCGGCGGCTGTCCAGGTCTGGTGCCTTTGTCGAACCTTGTAAAGCCTCgatga                                                                                                            <  1:287533/116‑1 (MQ=255)                               gatgatGTCGTTACCACCGGAAGTACCGTCGCAGAGATTGCGCAGTTGCTTTTACGCAATGGTGCGGCGGCTGTCCAGGTCTGGTGCCTTTGTCGAACCTTGTAAAGCCTCgatga                                                                                                            >  2:287533/1‑116 (MQ=255)                               gatgatGTCGTTACCACCGGAAGTACCGTCGCAGAGATTGCGCAGTTGCTTTTACGCAATGGTGCGGCGGCTGTCCAGGTCTGGTGCCTTTGTCGAACCTTGTAAAGCCTCGATGATGGGCGTATTATAACCAACTaaa                                                                                     >  2:442392/1‑139 (MQ=255)                                  gatgTCGTTACCACCGGAAGTACCGTCGCAGAGATTGCGCAGTTGCTTTTACGCAATGGTGCGGCGGCTGTCCAGGTCTGGTGCCTTTGTCGAACCTTGTAAAGCCTCGATGATGGGCGTATTATAACCAACTAAAATa                                                                                  >  1:278917/1‑139 (MQ=255)                                     gTCGTTACCACCGGAAGTACCGTCGCAGAGATTGCGCAGTTGCTTTTACGCAATGGTGCGGCGGCTGTCCAGGTCTGGTGCCTTTGTCGAACCTTGTAAAGCCTCGATGATGGGCGTATTATAACCAACTAAAATAGTc                                                                               <  2:478622/139‑1 (MQ=255)                                     gTCGTTACCACCGGAAGTACCGTCGCAGAGATTGCGCAGTTGCTTTTACGCAATGGTGCGGCGGCTGTCCAGGTCTGGTGCCTTTGTCGAACCTTGTAAAGCCTCGATGATGGGCGTATTATAACCAACTAAAATAGTc                                                                               <  1:442392/139‑1 (MQ=255)                                       cGTTACCACCGGAAGTACCGTCGCAGAGATTGCGCAGTTGCTTTTACGCAATGGTGCGGCGGCTGTCCACGTCTGGTGCCTTTGTCGAACCTTGTAAAGCCTCGATGATGGGCGTATCATAACCAACTAAAATAGTCaa                                                                             >  1:588455/1‑139 (MQ=255)                                         ttACCACCGGAAGTACCGTCGCAGAGATTGCGCAGTTGCTTTTACGCAATGGTGCGGCGGCTGTCCAGGTCTGGTGCCTTTGTCGAACCTTGTAAAGCCTCgatga                                                                                                            >  2:213993/1‑106 (MQ=255)                                         ttACCACCGGAAGTACCGTCGCAGAGATTGCGCAGTTGCTTTTACGCAATGGTGCGGCGGCTGTCCAGGTCTGGTGCCTTTGTCGAACCTTGTAAAGCCTCgatga                                                                                                            <  1:213993/106‑1 (MQ=255)                                                cGGAAGTACCGTCGCAGAGATTGCGCAGTTGCTTTTACGCAATGGTGCGGCGGCTGTCCAGGTCTGGTGCCTTTGTCGAACCTTGTAAAGCCTCGATGATggg                                                                                                        >  1:552092/1‑103 (MQ=255)                                                cGGAAGTACCGTCGCAGAGATTGCGCAGTTGCTTTTACGCAATGGTGCGGCGGCTGTCCAGGTCTGGTGCCTTTGTCGAACCTTGTAAAGCCTCGATGATggg                                                                                                        <  2:552092/103‑1 (MQ=255)                                                aggAAGTACCGTCGCAGAGATTGCGCAGTTGCTTTTACGCAATGGTGCGGCGGCTGTCCAGGTCTGGTGCCTTTGTCGAACCTTGTAAAGCCTCGATGATGGGCGTATTATAACCAACTAAAATAGTCAACTATTAGGc                                                                    <  1:86106/138‑1 (MQ=255)                                                  gAAGTACCGTCGCAGAGATTGCGCAGTTGCTTTTACGCAATGGTGCGGCGGCTGTCCAGGTCTGGTGCCTTTGTCGAACCTTGTAAAGCCTCGATGATggg                                                                                                        >  1:550158/1‑101 (MQ=255)                                                  gAAGTACCGTCGCAGAGATTGCGCAGTTGCTTTTACGCAATGGTGCGGCGGCTGTCCAGGTCTGGTGCCTTTGTCGAACCTTGTAAAGCCTCGATGATggg                                                                                                        <  2:550158/101‑1 (MQ=255)                                                  gAAGTACCGTCGCAGAGATTGCGCAGTTGCTTTTACGCAATGGTGCGGCGGCTGTCCAGGTCTGGTGCCTTTGTCGAACCTTGTAAAGCCTCGATGATGGGCGTATTATAACCAACTAAAATAGTCAACTATTAGGCCa                                                                  <  1:968793/139‑1 (MQ=255)                                                  gAAGTACCGTCGCAGAGATTGCGCAGTTGCTTTTACGCAATGGTGCGGCGGCTGTCCAGGTCTGGTGCCTTTGTCGAACCTTGTAAAGCCTCGATGATGGGCGTATTATAACCAACTAAAATAGTCAACTATTAGGCCa                                                                  <  1:77288/139‑1 (MQ=255)                                                   aaGTACCGTCGCAGAGATTGCGCAGTTGCTTTTACGCAATGGTGCGGCGGCTGTCCAGGTCTGGTGCCTTTGTCGAACCTTGTAAAGCCTCGATGATGGGCGTATTATAACCAACTAAAATAGTCAACTATTAGGCCAt                                                                 <  1:522467/139‑1 (MQ=255)                                                       aCCGTCGCAGAGATTGCGCAGTTGCTTTTACGCGATGGTGCGGCGTCTGTCCAGGTCTGGTGCCGTTGTCGAACCTTGTAAAGCCTCgatgat                                                                                                           <  2:406230/93‑1 (MQ=255)                                                       aCCGTCGCAGAGATTGCGCAGTTGCTTTTACGCAATGGTGCGGCGGCTGTCCAGGTCTGGTGCCTTTGTCGAACCTTGTAAAGCCTCgatgat                                                                                                           >  1:406230/1‑93 (MQ=255)                                                       aCCGTCGCAGAGATTGCGCAGTTGCTTTTACGCAATGGTGCGGCGGCTGTCCAGGTCTGGTGCCTTTGTCGAACCTTGTAAAGCCTCGATGATGGGCGTATTATAACCAACTAAAATAGTCAACTATTAGGCCATTACt                                                             <  2:588455/139‑1 (MQ=255)                                                          gTCGCAGAGATTGCGCAGTTGCTTTTACGCAATGGTGCGGCGGCTGTCCAGGTCTGGTGCCTTTGTCGAACCTTGTAAAGCCTCGATGATGGGCGTATTATAACCAACTAAAATAGTCAACTATTAGGc                                                                    >  1:868734/1‑129 (MQ=255)                                                          gTCGCAGAGATTGCGCAGTTGCTTTTACGCAATGGTGCGGCGGCTGTCCAGGTCTGGTGCCTTTGTCGAACCTTGTAAAGCCTCGATGATGGGCGTATTATAACCAACTAAAATAGTCAACTATTAGGc                                                                    <  2:868734/129‑1 (MQ=255)                                                           tCGCAGAGATTGCGCAGTTGCTTTTACGCAATGGTGCGGCGGCTGTCCAGGTCTGGTGCCTTTGTCGAACCTTGTAAAGCCTCGATGATGGGCGTATTATAACCAACTAAAATAGTCAACTATTAGGCCATTACTATGa                                                         <  2:278917/139‑1 (MQ=255)                                                            cGCAGAGATTGCGCAGTTGCTTTTACGCAATGGTGCGGCGGCTGTCCAGGTCTGGTGCCTTTGTCGAACCTTGTAAAGCCTCGATGATGGGCGTATTATAACCAACTAAAATAGTCAACTATTAGGCCATTACTATGAt                                                        >  2:785222/1‑139 (MQ=255)                                                             ggagagATTGCGCAGTTGCTTTTACGCAATGGTGCGGCGGCTGTCCAGGTCTGGTGCCTTTGTCGAACCTTGTAAAGCCTCGATGATGGGCGTATTATAACCAACTAAAATAGTc                                                                               <  2:652877/113‑1 (MQ=255)                                                             gCAGAGATTGCGCAGTTGCTTTTACGCAATGGTGCGGCGGCTGTCCAGGTCTGGTGCCTTTATCGAACCTTGTAAAGCCTCGATGATGGGCGTATTATAACCAACTAAAGTAGTc                                                                               >  1:652877/1‑115 (MQ=255)                                                                            ttGCTTTTACGCAATGGTGCGGCGGCTGTCCAGGTCTGGTGCCTTTGTCGAACCTTGTAAAGCCt                                                                                                                  <  1:501219/65‑1 (MQ=255)                                                                            ttGCTTTTACGCAATGGTGCGGCGGCTGTCCAGGTCTGGTGCCTTTGTCGAACCTTGTAAAGCCt                                                                                                                  >  2:501219/1‑65 (MQ=255)                                                                             tGCTTTTACGCAATGGTGCGGCGGCTGTCCAGGTCTGGTGCCTTTGTCGAACCTTGTAAAGCCTCGATGATGGGCGTATTATAACCAACTAAAATAGTCAACTATTAGGCCATTACTATGATCCGt                                                    >  1:86422/1‑126 (MQ=255)                                                                             tGCTTTTACGCAATGGTGCGGCGGCTGTCCAGGTCTGGTGCCTTTGTCGAACCTTGTAAAGCCTCGATGATGGGCGTATTATAACCAACTAAAATAGTCAACTATTAGGCCATTACTATGATCCGt                                                    <  2:86422/126‑1 (MQ=255)                                                                             tGCTTTTACGCAATGGTGCGGCGGCTGTCCAGGTCTGGTGCCTTTGTCGAACCTTGTAAAGCCTCGATGATGGGCGTATTATAACCAACTAAAATAGTCAACTATTAGGCCATTACTATGATCCGTATTTCCGAtgctg                                       <  1:204663/139‑1 (MQ=255)                                                                                       cAATGGTGCGGCGGCTGTCCAGGTCTGGTGCCTTTGTCGAACCTTGTAAAGCCTCGATGATGGGCGTATTATAACCAACTAAAATAGTCAACTATTAGGCCATTACTATGATCCGTATTTCCGATGCTGCACAAGCGCa                             <  1:785222/139‑1 (MQ=255)                                                                                         aTGGTGCGGCGGCTGTCCAGGTCTGGTGCCTTTGTCGAACCTTGTAAAGCCTCGATGATGGGCGTATTATAACCAACTAAAATAGTCAACTATTAGGCCATTACTATGATCCGTATTTCCGATGCTGCACAAGCGCACt                           <  2:189944/139‑1 (MQ=255)                                                                                            gTGCGGCGGCTGTCCAGGTCTGGTGCCTTTGTCGAACCTTGTAAAGCCTCGATGATGGGc                                                                                                       >  2:657996/1‑60 (MQ=255)                                                                                            gTGCGGCGGCTGTCCAGGTCTGGTGCCTTTGTCGAACCTTGTAAAGCCTCGATGATGGGc                                                                                                       <  1:657996/60‑1 (MQ=255)                                                                                               cggcggCTGTCCAGGTCTGGTGCCTTTGTCGAACCTTGTAAAGCCTCGATGATGGGCGTATTATAACCAACTAAAATAGTCAACTATTAGGCCATTACTATGATCCGTATTTCCGATGCTGCACAAGCGCACTTTGCCa                     >  2:302135/1‑139 (MQ=255)                                                                                                    gcTGTCCAGGTCTGGTGCCTTTGTCGAACCTTGTAAAGCCTCGATGATGGGCGTATTATAACCAACTAAAATAGTCa                                                                              >  1:939955/1‑77 (MQ=255)                                                                                                    gcTGTCCAGGTCTGGTGCCTTTGTCGAACCTTGTAAAGCCTCGATGATGGGCGTATTATAACCAACTAAAATAGTCa                                                                              <  2:939955/77‑1 (MQ=255)                                                                                                     cTGTCCAGGTCTGGTGCCTTTGTCGAACCTTGTAAAGCCTCGATGATGGGCGTATTATAACCAACTAAAATAGTCAACTATTAGGCCATTACTATGATCCGTATTTCCGATGCTGCACAAGCGCACTTTGCCAAActgc               >  1:764359/1‑139 (MQ=255)                                                                                                                  gTGCCTTTGTCGAACCTTGTAAAGCCTCGTTGATGGGCGTATTATAACCAACTaaaa                                                                                    <  1:926372/57‑1 (MQ=255)                                                                                                                  gTGCCTTTGTCGAACCTTGTAAAGCCTCGTTGATGGGCGTATTATAACCAACTaaaa                                                                                    >  2:926372/1‑57 (MQ=255)                                                                                                                  gTGCCTTTGTCGAACCTTGTAAAGCCTCGATGATGGGCGTATTATAACCAACTAAAATAGTCAACTATTAGGc                                                                    >  2:692347/1‑73 (MQ=255)                                                                                                                  gTGCCTTTGTCGAACCTTGTAAAGCCTCGATGATGGGCGTATTATAACCAACTAAAATAGTCAACTATTAGGc                                                                    <  1:692347/73‑1 (MQ=255)                                                                                                                  gTGCCTTTGTCGAACCTTGTAAAGCCTCGATGATGGGCGTATTATAACCAACTAAAATAGTCAACTATTAGGCCATTACTATGATCCGTATTTCCGAtg                                          >  2:36693/1‑99 (MQ=255)                                                                                                                  gTGCCTTTGTCGAACCTTGTAAAGCCTCGATGATGGGCGTATTATAACCAACTAAAATAGTCAACTATTAGGCCATTACTATGATCCGTATTTCCGAtg                                          <  1:36693/99‑1 (MQ=255)                                                                                                                  gTGCCTTTGTCGAACCTTGTAAAGCCTCGATGATGGGCGTATTATAACCAACTAAAATAGTCAACTATTAGGCCATTACTATGATCCGTATTTCCGATGCTGCACAAGCGCACTTTGCCAAACTGCTGGCAAATCAGga  >  2:162748/1‑139 (MQ=255)                                                                                                                                    gTAAAGCCTCGATGATGGGCGTATTATAACCAACTAAAATAGTCAACTATTAGGc                                                                    <  2:985038/55‑1 (MQ=255)                                                                                                                                       |                                                                                                                      CCCGTGCAAGGTCGCCATATGGTGATTGTGGATGATGTCGTTACCACCGGAAGTACCGTCGCAGAGATTGCGCAGTTGCTTTTACGCAATGGTGCGGCGGCTGTCCAGGTCTGGTGCCTTTGTCGAACCTTGTAGAGCCTCGATGATGGGCGTATTATAACCAACTAAAATAGTCAACTATTAGGCCATTACTATGATCCGTATTTCCGATGCTGCACAAGCGCACTTTGCCAAACTGCTGGCAAATCAGGA  >  NC_000913/3545431‑3545682

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑