Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,676,878 G→A P491P (CCC→CCT)  yhjG ← putative inner membrane‑anchored periplasmic AsmA family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,676,8780GA100.0% 113.4 / NA 36P491P (CCC→CCT) yhjGputative inner membrane‑anchored periplasmic AsmA family protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (19/17);  total (19/17)

ACCAGCCCGTCATTCATCAACAGTTTCAGGTTGCCGTTACTGTTGCCTAAAAGTGCCGCCACCGAGTTACCGCTACCGCGTAGTTCCGCGTCACCGTTCATTTCCCCCAGCGTCTTCTGCATCAGTTCCACATCGGGCATCAGTTCTTTCAGTTTCAGTCGACGAGCCTGAATATCTGCCCGCCCCTGCATCGGCTTTTTATCGCCTTCCAGATGAATATTCGCCGCAATGCTGCCGCCCGCCATGCCAAATTTCAGCGGTTGCAGGCG  >  NC_000913/3676744‑3677012
                                                                                                                                      |                                                                                                                                      
aCCAGCCCGTCATTCATCAACAGTTTCAGGTTGCCGTTACTGTTGCCTAAAAGTGCCGCCACCGAGTTACCGCTACCGCGTAGTTCCGCGTCACCGTTCATTTCCCCCAGCGTCTTCTGCATCAGTTCCACATCAGGCa                                                                                                                                    <  1:353468/139‑1 (MQ=255)
aCCAGCCCGTCATTCATCAACAGTTTCAGGTTGCCGTTACTGTTGCCTAAAAGTGCCGCCACCGAGTTACCGCTACCGCGTAGTTCCGCGTCACCGTTCATTTCCCCCAGCGTCTTCTGCATCAGTTCCACATCAGGCa                                                                                                                                    >  2:511952/1‑139 (MQ=255)
aCCAGCCCGTCATTCATCAACAGTTTCAGGTTGCCGTTACTGTTGCCTAAAAGTGCCGCCACCGAGTTACCGCTACCGCGTAGTTCCGCGTCACCGTTCATTTCCCCCAGCGTCTTCTGCATCAGTTCCACATCAGGCa                                                                                                                                    >  1:783136/1‑139 (MQ=255)
   aGCCCGTCATTCATCAACAGTTTCAGGTTGCCGTTACTGTTGCCTAAAAGTGCCGCCACCGAGTTACCGCTACCGCGTAGTTCCGCGTCACCGTTCATTTCCCCCAGCGTCTTCTGCATCAGTTCCACATCAGGCATCa                                                                                                                                 <  2:192149/139‑1 (MQ=255)
    gCCCGTCATTCATCAACAGTTTCAGGTTGCCGTTACTGTTGCCTAAAAGTGCCGCCACCGAGTTACCGCTACCGCGTAGTTCCGCGTCACCGTTCATTTCCCCCAGCGTCTTCTGCATCAGTTCCACATCAGGCATCAg                                                                                                                                <  2:490411/139‑1 (MQ=255)
     cccGTCATTCATCAACAGTTTCAGGTTGCCGTTACTGTTGCCTAAAAGTGCCGCCACCGAGTTACCGCTACCGCGTAGTTCCGCGTCACCGTTCATTTCCCCCAGCGTCTTCTGCATCAGTTCCACATCAGGCATCAGt                                                                                                                               >  2:871500/1‑139 (MQ=255)
                       tttCAGGTTGCCGTTACTGTTGCCTAAAAGTGCCGCCACCGAGTTACCGCTACCGCGTAGTTCCGCGTCACCGTTCATTTCCCCCAGCGTCTTCTGCATCAGTTCCACATCAGGCATCAGTTCTTTCAGTTTCAGTcga                                                                                                             <  2:783136/139‑1 (MQ=255)
                       tttCAGGTTGCCGTTACTGTTGCCTAAAAGTGCCGCCACCGAGTTACCGCTACCGCGTAGTTCCGCGTCACCGTTCATTTCCCCCAGCGTCTTCTGCATCAGTTCCACATCAGGCATCAGTTCTTTCAGTTTCAGTcga                                                                                                             >  2:484265/1‑139 (MQ=255)
                        ttCAGGTTGCCGTTACTGTTGCCTAAAAGTGCCGCCACCGAGTTACCGCTACCGCGTAGTTCCGCGTCACCGTTCATTTCCCCCAGCGTCTTCTGCATCAGTTCCACATCAGGCATCAGTTCTTTCAGTTTCAGTcgac                                                                                                            <  2:346016/139‑1 (MQ=255)
                          cAGGTTGCCGTTACTGTTGCCTAAAAGTGCCGCCACCGAGTTACCGCTACCGCGTAGTTCCGCGTCACCGTTCATTTCCCCCAGCGTCTTCTGCATCAGTTCCACATCAGGCATCAGTTCTTTCAGTTTCAGTcgacga                                                                                                          <  1:508228/139‑1 (MQ=255)
                                 ccGTTACTGTTGCCTAAAAGTGCCGCCACCGAGTTACCGCTACCGCGTAGTTCCGCGTCACCGTTCATTTCCCCCAGCGTCTTCTGCATCAGTTCCACATCAGGCATCAGTTCTTTCAGTTTCAGTcgacg                                                                                                           <  2:552697/131‑1 (MQ=255)
                                 ccGTTACTGTTGCCTAAAAGTGCCGCCACCGAGTTACCGCTACCGCGTAGTTCCGCGTCACCGTTCATTTCCCCCAGCGTCTTCTGCATCAGTTCCACATCAGGCATCAGTTCTTTCAGTTTCAGTcgacg                                                                                                           >  1:552697/1‑131 (MQ=255)
                                         gTTGCCTAAAAGTGCCGCCACCGAGTTACCGCTACCGCGTAGTTCCGCGTCACCGTTCATTTCCCCCAGCGTCTTCTGCATCAGTTCCACATCAGGCATCAGTTCTTTCAGTTTCAGTCGACGAGCCTGAATATCTgcc                                                                                           <  1:484265/139‑1 (MQ=255)
                                                    ataatagacaggAGTTACCGCTTCCCCCGAGTTCCCCGTTAACTTTCATTTCCCCCAGCGTCTTCTGCATCAGTTCCACATCAGGCATCAGTTCTTTCAGTTTCAGTCGACGAGCCTGAATATCTGCCCGCCCCTGCAt                                                                                <  2:922067/128‑1 (MQ=255)
                                                       ccgccACCGAGTTACCGCTACCGCGTAGTTCCGCGTCACCGTTCATTTCCCCCAGCGTCTTCTGCATCAGTTCCACATCAGGCATCAGTTCTTTCAGTTTCAGTcgacga                                                                                                          >  2:16979/1‑110 (MQ=255)
                                                       ccgccACCGAGTTACCGCTACCGCGTAGTTCCGCGTCACCGTTCATTTCCCCCAGCGTCTTCTGCATCAGTTCCACATCAGGCATCAGTTCTTTCAGTTTCAGTcgacga                                                                                                          <  1:16979/110‑1 (MQ=255)
                                                               gAGTTACCGCTACCGCGTAGTTCCGCGTCACCGTTCATTTCCCCCAGCGTCTTCTGCATCAGTTCCACATCAGGCATCAGTTCTTTCAGTTTCAGTCGACGAGCCTGAATATCTGCCCGCCCCTGCATCGGCTTTTTAt                                                                     <  2:987110/139‑1 (MQ=255)
                                                                          accgcGTAGTTCCGCGTCACCGTTCATTTCCCCCGCCGTCTTCTGCATCAGTTCCCAATAAGGAATCAGTTCTTCCGGTTCAAGCGGACGAGCAGGAATATCTGCCCGCCCCGCAATCGGCTTTTTTTctgtctcttat                                                          >  1:922067/1‑129 (MQ=255)
                                                                           ccgcGTAGTTCCGCGTCACCGTTCATTTCCCCCAGCGTCTTCTGCATCAGTTCCACATCAGGCATCAGTTCTTTCAGTTTCAGTCGACGAGCCTGAATATCTGCCCGCCCCTGCATCGGCTTTTTATCGCCTTCCAGAt                                                         >  1:566357/1‑139 (MQ=255)
                                                                                     ccGCGTCACCGTTCATTTCCCCCAGCGTCTTCTGCATCAGTTCCACATCAGGCATCAGTTCTTTCAGTTTCAGTCGACGAGCCTGAATATCTGCCCGCCCCTGCATCGGCTTTTTATCGCCTTCCAGATGAATATTcgc                                               <  1:871500/139‑1 (MQ=255)
                                                                                     ccGCGTCACCGTTCATTTCCCCCAGCGTCTTCTGCATCAGTTCCACATCAGGCATCAGTTCTTTCAGTTTCAGTCGACGAGCCTGAATATCTGCCCGCCCCTGCATCGGCTTTTTATCGCCTTCCAGATGAATATTcgc                                               >  2:560991/1‑139 (MQ=255)
                                                                                        cgTCACCGTTCATTTCCCCCAGCGTCTTCTGCATCAGTTCCACATCAGGCATCAGTTCTTTCAGTTTCAGTCGACGAGCCTGAATATCTGCCCGCCCCTGCATCGGCTTTTTATCGCCTTCCAGATGAATATTcgccgc                                            >  2:948612/1‑139 (MQ=255)
                                                                                                       cccccAGCGTCTTCTGCATCAGTTCCACATCAGGCATCAGTTCTTTCAGTTTCAGTCGACGAGc                                                                                                        <  1:911024/64‑1 (MQ=255)
                                                                                                       cccccAGCGTCTTCTGCATCAGTTCCACATCAGGCATCAGTTCTTTCAGTTTCAGTCGACGAGc                                                                                                        >  2:911024/1‑64 (MQ=255)
                                                                                                          ccAGCGTCTTCTGCATCAGTTCCACATCAGGCATCAGTTCTTTCAGTTTCAGTcga                                                                                                             <  2:471220/56‑1 (MQ=255)
                                                                                                          ccAGCGTCTTCTGCATCAGTTCCACATCAGGCATCAGTTCTTTCAGTTTCAGTcga                                                                                                             >  1:471220/1‑56 (MQ=255)
                                                                                                          ccAGCGTCTTCTGCATCAGTTCCACATCAGGCATCAGTTCTTTCAGTTTCAGTCGACGAGCCTGAATATCTGCCCGCCCCTGCATCGGCTTTTTATCGCCTTCCAGATGAATATTCGCCGCAATGCTGCCGCCCGCCAt                          >  1:351394/1‑139 (MQ=255)
                                                                                                                 cttctGCATCAGTTCCACATCAGGCATCAGTTCTTTCAGTTTCAGTCGACGAGCCTGAATATCTGCCCGCCCCTGCATCGGCTTTTTATCGCCTTCCAGATGAATATTCGCCGCAATGCTGCCGCCCGCCATGCCAAAt                   >  1:1009274/1‑139 (MQ=255)
                                                                                                                            gTTCCACATCAGGCATCAGTTCTTTCAGTTTCAGTCGACGAGCCTGAATATCTGCCCGCCCCTGCATCGGCTTTTTATCGCCTTc                                                              >  2:462564/1‑85 (MQ=255)
                                                                                                                            gTTCCACATCAGGCATCAGTTCTTTCAGTTTCAGTCGACGAGCCTGAATATCTGCCCGCCCCTGCATCGGCTTTTTATCGCCTTc                                                              <  1:462564/85‑1 (MQ=255)
                                                                                                                             ttCCACATCAGGCATCAGTTCTTTCAGTTTCAGTCGACGAGCCTGAATATCTGCCCGCCCCTGCATCGGCTTTTTAt                                                                     <  1:973411/77‑1 (MQ=255)
                                                                                                                             ttCCACATCAGGCATCAGTTCTTTCAGTTTCAGTCGACGAGCCTGAATATCTGCCCGCCCCTGCATCGGCTTTTTAt                                                                     >  2:973411/1‑77 (MQ=255)
                                                                                                                             ttCCACATCAGGCATCAGTTCTTTCAGTTTCAGTCGACGAGCCTGAATATCTGCCCGCCCCTGCATCGGCTTTTTATCGCCTTCCAGATGAATATTCGCCGCAATGCTGCCGCCCGCCATGCCAAATTTCAGCGGTTGc       >  1:525370/1‑139 (MQ=255)
                                                                                                                             ttCCACATCAGGCATCAGTTCTTTCAGTTTCAGTCGACGAGCCTGAATATCTGCCCGCCCCTGCATCGGCTTTTTATCGCCTTCCAGATGAATATTCGCCGCAATGCTGCCGCCCGCCATGCCAAATTTCAGCGGTTGc       >  1:782706/1‑139 (MQ=255)
                                                                                                                              tCCACATCAGGCATCAGTTCTTTCAGTTTCAGTCGACGAGCCTGAATATCTGCCCGCCCCTGCATCGGCTTTTTATCGCCTTCCAGATGAATATTcgc                                               >  1:514136/1‑98 (MQ=255)
                                                                                                                              tCCACATCAGGCATCAGTTCTTTCAGTTTCAGTCGACGAGCCTGAATATCTGCCCGCCCCTGCATCGGCTTTTTATCGCCTTCCAGATGAATATTcgc                                               <  2:514136/98‑1 (MQ=255)
                                                                                                                                  caTCAGGGATCAGTTCTTTCAGTTTCAGTCGACGAGCCTGAATATCTGCCCGCCCCTGCATCTGCTTTTTATCGCCTTCCAGATGAATATTCGCCGCAATGCTGCCGCCCGCCATGCCAGATTTCAGCGGTTGCAGgcg  >  1:661790/1‑139 (MQ=255)
                                                                                                                                      |                                                                                                                                      
ACCAGCCCGTCATTCATCAACAGTTTCAGGTTGCCGTTACTGTTGCCTAAAAGTGCCGCCACCGAGTTACCGCTACCGCGTAGTTCCGCGTCACCGTTCATTTCCCCCAGCGTCTTCTGCATCAGTTCCACATCGGGCATCAGTTCTTTCAGTTTCAGTCGACGAGCCTGAATATCTGCCCGCCCCTGCATCGGCTTTTTATCGCCTTCCAGATGAATATTCGCCGCAATGCTGCCGCCCGCCATGCCAAATTTCAGCGGTTGCAGGCG  >  NC_000913/3676744‑3677012

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑