Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,057,170	C→T	A289T (GCA→ACA)	glnA ←	glutamine synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,057,170	0	C	T	100.0%	142.7 / NA	45	A289T (GCA→ACA)	glnA	glutamine synthetase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (26/19);  total (26/19)

CATTACCGGTGCTTCATAGCCCGGGACCAGACGCTTATAAGAGTTGGTGGTCGGGTTTGCCAGGGCGTTAATCGCTTTAGCGTGTTTGATTACGCCGCCAATGTAGTACAGCGCCTGCTCAGACAGACCTGCGTATTTGTCGCCTGCGAACAGGTTAACGCCGTTTTTAGACAGAGACATGTGGCAGTGCATACCGGAGCCGTTATCACCGAACATCGGTTTTGGCATAAAGGTCGCGGTTTTACCGAAGCGGTGCGCTACGTTGT  >  NC_000913/4057039‑4057304                                                                                                                                    |                                                                                                                                       cATTACCGGTGCTTCATAGCCCGGGACCAGACGCTTATAAGAGCTGGTGGTCGGGTTTGCCAGGGCGTTAATCGCTTTAGCGTGTTTGATTACGCCGCCAATGAAGTACAGCGCCTGCTCAGACAGACCTGTGTCTTTg                                                                                                                                 >  2:115417/1‑139 (MQ=255)               cATAGCCCGGGACCAGACGCTTATAAGAGTTGGTGGTCGGGTTTGCCAGGGCGTTAATCGCTTTAGCGTGTTTGATTACGCCGCCAATGTAGTACAGCGCCTGCTCAGACAGACCTGTGTATTTGTCGCCTGCGAACAg                                                                                                                   >  2:701427/1‑139 (MQ=255)                              gACGTTTATAAGAGTTGGTGGTCGGGTTTGCCAGGGCGTTAATCGCTTTAGCGTGTATGATTACGCCTCTAATGTATTACAGCGCCTGCTCAGGCAGACCTGTGTATTTGTCGCCTGCGAACAGGTTAACGCCGttttt                                                                                                    <  1:729796/139‑1 (MQ=255)                              gACGCTTATAAGAGTTGGTGGTCGGGTTTGCCAGGGCGTTAATCGCTTTAGCGCGTTTGATTACGCCGCCAATGTAGTACAGCGCCTGCTCAGACAGACCTGTGTATTTGTCGCCTGCGAACAGGTTAACGCCGttttt                                                                                                    <  2:199138/139‑1 (MQ=255)                                    tataAGAGTTGGTGGTCGGGTTTGCCAGGGCGTTAATCGCTTTAGCGTGTTTGATTACGCCGCCAATGTAGTACAGCGCCTGCTCAGACAGACCTGTGTATTTGTCGCCTGCGAACAGGTTAACGCCGTTTTTAGACag                                                                                              <  1:701427/139‑1 (MQ=255)                                      taAGAGTTGGTGGTCGGGTTTGCCAGGGCGTTAATCGCTTTAGCGTGTTTGATTACGCCGCCAATGTAGTACAGCGCCTGCTCAGACAGACCTGTGTATTTGTCGCCTGCGAACAGGTTAACGCCGTTTTTAGACagag                                                                                            >  2:966157/1‑139 (MQ=255)                                             tggtggTCGGGTTTGCCAGGGCGTTAATCGCTTTAGCGTGTTTGATTACGCCGCCAATGTAGTACAGCGCCTGCTCAGACAGACCTGTGTATTTGTCGCCTGCGAACAGGTTAACGCCGTTTTTAGACAGAGACAtgtg                                                                                     >  1:698162/1‑139 (MQ=255)                                             tggtggTCGGGTTTGCCAGGGCGTTAATCGCTTTAGCGTGTTTGATTACGCCGCCAATGTAGTACAGCGCCTGCTCAGACAGACCTGTGTATTTGTCGCCTGCGAACAGGTTAACGCCGTTTTTAGACAGAGACAtgtg                                                                                     >  2:406538/1‑139 (MQ=255)                                              ggtggtCGGGTTTGCCAGGGCGTTAATCGCTTTAGCGTGTTTGATTACGCCGCCAATGTAGTACAGCGCCTGCTCAGACAGACCTGTGTATTTGTCGCCTGCGAACAGGTTAACGCCGTTTTTAGACAGAGACATGTgg                                                                                    <  1:546796/139‑1 (MQ=255)                                              ggtggtCGGGTTTGCCAGGGCGTTAATCGCTTTAGCGTGTTTGATTACGCCGCCAATGTAGTACAGCGCCTGCTCAGACAGACCTGTGTATTTGTCGCCTGCGAACAGGTTAACGCCGTTTTTAGACAGAGACATGTgg                                                                                    <  2:521613/139‑1 (MQ=255)                                              ggtggtCGGGTTTGCCAGGGCGTTAATCGCTTTAGCGTGTTTGATTACGCCGCCAATGTAGTACAGCGCCTGCTCAGACAGACCTGTGTATTTGTCGCCTGCGAACAGGTTAACGCCGTTTTTAGACAGAGACATGTgg                                                                                    >  2:189246/1‑139 (MQ=255)                                              ggtggtCGGGTTTGCCAGGGCGTTAATCGCTTTAGCGTGTTTGATTACGCCGCCAATGTAGTACAGCGCCTGCTCAGACAGACCTGTGTATTTGTCGCCTGCGAACAGGTTAACGCCGTTTTTAGACAGAGACATGTgg                                                                                    <  1:966157/139‑1 (MQ=255)                                               gtggtCGGGTTTGCCAGGGCGTTAATCGCTTTAGCGTGTTTGATTACGCCGCCAATGTAGTACAGCGCCTGCTCAGACAGACCTGTGTATTTGTCGCCTGCGAACAGGTTAACGCCGTTTTTAGACAGAGACATGTGGc                                                                                   >  2:895060/1‑139 (MQ=255)                                                                ggCGTTAATCGCTTTAGCGTGTTTGATTACGCCGCCAATGTAGTACAGCGCCTGCTCAGACAGACCTGTGTATTTGTCGCCTGCGAACAGGTTAACGCCGttt                                                                                                      <  2:863982/103‑1 (MQ=255)                                                                ggCGTTAATCGCTTTAGCGTGTTTGATTACGCCGCCAATGTAGTACAGCGCCTGCTCAGACAGACCTGTGTATTTGTCGCCTGCGAACAGGTTAACGCCGttt                                                                                                      >  1:863982/1‑103 (MQ=255)                                                                 gCGTTAATCGCTTTAGCGTGTTTGATTACGCCGCCAATGTAGTACAGCGCCTGCTCAGACAGACCTGTGTATTTGTCGCCTGCGAACAGGTTAACGCCGTTTTTAGACAGAGACa                                                                                         <  2:202114/115‑1 (MQ=255)                                                                 gCGTTAATCGCTTTAGCGTGTTTGATTACGCCGCCAATGTAGTACAGCGCCTGCTCAGACAGACCTGTGTATTTGTCGCCTGCGAACAGGTTAACGCCGTTTTTAGACAGAGACa                                                                                         >  1:202114/1‑115 (MQ=255)                                                                 gCGTTAATCGCTTTAGCGTGTTTGATTACGCCGCCAATGTAGTACAGCGCCTGCTCAGACAGACCTGTGTATTTGTCGCCTGCGAACAGGTTAACGCCGTTTTTAGACAGAGACATGTGGCAGTGCATAc                                                                          <  1:640216/130‑1 (MQ=255)                                                                 gCGTTAATCGCTTTAGCGTGTTTGATTACGCCGCCAATGTAGTACAGCGCCTGCTCAGACAGACCTGTGTATTTGTCGCCTGCGAACAGGTTAACGCCGTTTTTAGACAGAGACATGTGGCAGTGCATAc                                                                          >  2:640216/1‑130 (MQ=255)                                                                                     tttGATTACGCCGCCAATGTAGTACAGCGCCTGCTCAGACAGACCTGTGTATTTGTCGCCTGCGAACAGGTTAACGCCGTTTTTAGACAGAGACATGTGGCAGTGCATACCGGAGCCGTTATCACCGAACATCGGtttt                                             >  2:329954/1‑139 (MQ=255)                                                                                           tACGCCGCCAATGTAGTACAGCGCCTGCTCAGACAGACCTGTGTATTTGTCGCCTGCGAACAGGTTAACGCCGTTTTTAGACAGAGACATGTGGCAGTGCATACCGGAGCCGTTATCACCGAACATCGGTTTTGGCATa                                       >  1:167549/1‑139 (MQ=255)                                                                                             cgccgcCAATGTAGTACAGCGCCTGCTCAGACAGACCTGTGTATTTGTCGCCTGCGAACAGGTTAACGCCGTTTTTAGACAGAGACAtgtg                                                                                     <  2:122764/91‑1 (MQ=255)                                                                                             cgccgcCAATGTAGTACAGCGCCTGCTCAGACAGACCTGTGTATTTGTCGCCTGCGAACAGGTTAACGCCGTTTTTAGACAGAGACAtgtg                                                                                     >  1:122764/1‑91 (MQ=255)                                                                                                  ccAATGTAGTACAGCGCCTGCTCAGACAGACCTGTGTATTTGTCGCCTGCGAACAGGTTAACGCCGTTTTTAGACAGAGACATGTGGCAGTGCATACCGGAGCCGTTATCACCGAACATCGGTTTTGGCATAAAGGTcg                                >  2:138581/1‑139 (MQ=255)                                                                                                   cAATGTAGTACAGCGCCTGCTCAGACAGACCTGTGTATTTGTCGCCTGCGAACAGGTTAACGCCGTTTTTAGACAGAGACATGTGGCAGTGCATACCGGAGCCGTTATCACCGAACATCGGTTTTGGCATa                                       <  1:936803/131‑1 (MQ=255)                                                                                                   cAATGTAGTACAGCGCCTGCTCAGACAGACCTGTGTATTTGTCGCCTGCGAACAGGTTAACGCCGTTTTTAGACAGAGACATGTGGCAGTGCATACCGGAGCCGTTATCACCGAACATCGGTTTTGGCATa                                       >  2:936803/1‑131 (MQ=255)                                                                                                               gcgcCTGCTCAGACAGACCTGTGTATTTGTCGCCTGCGAACAGGTTAACGc                                                                                                           >  2:255970/1‑51 (MQ=255)                                                                                                               gcgcCTGCTCAGACAGACCTGTGTATTTGTCGCCTGCGAACAGGTTAACGc                                                                                                           <  1:255970/51‑1 (MQ=255)                                                                                                                cgcCTGCTCAGACAGACCTGTGTATTTGTCGCCTGCGAACAGGTTAACGCCGTTTTTAGACagag                                                                                            <  2:640701/65‑1 (MQ=255)                                                                                                                cgcCTGCTCAGACAGACCTGTGTATTTGTCGCCTGCGAACAGGTTAACGCCGTTTTTAGACagag                                                                                            >  1:640701/1‑65 (MQ=255)                                                                                                                cgcCTGCTCAGACAGACCTGTGTATTTGTCGCCTGCGAACAGGTTAACGCCGTTTTTAGACAGAGACATGTGGCAGTGCATACCGGa                                                                      <  1:872374/87‑1 (MQ=255)                                                                                                                cgcCTGCTCAGACAGACCTGTGTATTTGTCGCCTGCGAACAGGTTAACGCCGTTTTTAGACAGAGACATGTGGCAGTGCATACCGGa                                                                      >  2:872374/1‑87 (MQ=255)                                                                                                                cgcCTGCTCAGACAGACCTGTGTATTTGTCGCCTGCGAACAGGTTAACGCCGTTTTTAGACAGAGACATGTGGCAGTGCATACCGGAGCCGTTATCACCGAACATCGGttt                                              <  1:809102/111‑1 (MQ=255)                                                                                                                cgcCTGCTCAGACAGACCTGTGTATTTGTCGCCTGCGAACAGGTTAACGCCGTTTTTAGACAGAGACATGTGGCAGTGCATACCGGAGCCGTTATCACCGAACATCGGttt                                              >  2:809102/1‑111 (MQ=255)                                                                                                                cgcCTGCTCAGACAGACCTGTGTATTTGTCGCCTGCGAACAGGTTAACGCCGTTTTTAGACAGAGACATGTGGCAGTGCATACCGGAGCCGTTATCACCGAACATCGGtt                                               <  2:886551/110‑1 (MQ=255)                                                                                                                cgcCTGCTCAGACAGACCTGTGTATTTGTCGCCTGCGAACAGGTTAACGCCGTTTTTAGACAGAGACATGTGGCAGTGCATACCGGAGCCGTTATCACCGAACATCGGtt                                               <  2:486755/110‑1 (MQ=255)                                                                                                                cgcCTGCTCAGACAGACCTGTGTATTTGTCGCCTGCGAACAGGTTAACGCCGTTTTTAGACAGAGACATGTGGCAGTGCATACCGGAGCCGTTATCACCGAACATCGGtt                                               >  1:886551/1‑110 (MQ=255)                                                                                                                cgcCTGCTCAGACAGACCTGTGTATTTGTCGCCTGCGAACAGGTTAACGCCGTTTTTAGACAGAGACATGTGGCAGTGCATACCGGAGCCGTTATCACCGAACATCGGtt                                               >  1:486755/1‑110 (MQ=255)                                                                                                                  ccTGCTCAGACAGACCTGTGTATTTGTCGCCTGCGATCAGGTTAACGCCGTTTTTAGACAGAGACATGTGGCAGTGCATACCGGAGCCGTTATCACTGAACATCGGTTTTGGCATAAAGGTCGCGGTTTTACCGAAGCg                >  2:134430/1‑139 (MQ=255)                                                                                                                  ccTGCTCAGACAGACCTGTGTATTTGTCGCCTGCGAACAGGTTAACGCCGTTTTTAGACAGAGACATGTGGCAGTGCATACCGGAGCCGTTATCa                                                            >  2:929369/1‑95 (MQ=255)                                                                                                                  ccTGCTCAGACAGACCTGTGTATTTGTCGCCTGCGAACAGGTTAACGCCGTTTTTAGACAGAGACATGTGGCAGTGCATACCGGAGCCGTTATCa                                                            <  1:929369/95‑1 (MQ=255)                                                                                                                  ccTGCTCAGACAGACCTGTGTATTTGTCGCCTGCGAACAGGTTAACGCCGTTTTTAGACAGAGACATGTGGCAGTGCATACCGGAGCCGTTATCACCGAACATCGGTTTTGGCATAAAGGTCGCGGTTTTACCGAAGCg                >  1:616191/1‑139 (MQ=255)                                                                                                                       tCAGACAGACCTGTGTATTTGTCGCCTGCGAACAGGTTAACGCCGTTTTTAGACAGAGACATGTGGCAGTGCATACCGGAGCCGTTATCACCGAACATCGGTTTTGGCATAAAGGTCGCGGTTTTACCGAAGCGGTgcg           <  2:167549/139‑1 (MQ=255)                                                                                                                               acCTGTGTATTTGTCGCCTGCGAACAGGTTAACGCCGTTTTTAGACAGAGACATGTGGCAGTGCATACCGGAGCCGTTATCACCGAACATCGGTTTTGGCATAAAGGTCGCGGTTTTACCGAAGCGGTGCGCTACGTtg   >  2:616020/1‑139 (MQ=255)                                                                                                                                ccTGTGTATTTGTCGCCTGCGAACAGGTTAACGCCGTTTTTAGACAGAGACATGTGGCAGTGCATACCGGAGCCGTTATCACCGAACATCGGTTTTGGCATAAAGGTCGCGGTTTTACCGAAGCGGTGCGCTACGTtgt  >  1:690523/1‑139 (MQ=255)                                                                                                                                    |                                                                                                                                       CATTACCGGTGCTTCATAGCCCGGGACCAGACGCTTATAAGAGTTGGTGGTCGGGTTTGCCAGGGCGTTAATCGCTTTAGCGTGTTTGATTACGCCGCCAATGTAGTACAGCGCCTGCTCAGACAGACCTGCGTATTTGTCGCCTGCGAACAGGTTAACGCCGTTTTTAGACAGAGACATGTGGCAGTGCATACCGGAGCCGTTATCACCGAACATCGGTTTTGGCATAAAGGTCGCGGTTTTACCGAAGCGGTGCGCTACGTTGT  >  NC_000913/4057039‑4057304

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑