Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,137,229	A→G	S143S (AGT→AGC)	yijF ←	DUF1287 family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,137,229	0	A	G	100.0%	116.0 / NA	33	S143S (AGT→AGC)	yijF	DUF1287 family protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (18/15);  total (18/15)

CGCCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGCGAAGCCATCTGAAACCACCCCAATATGTGCCAGCCCGTTATCCAGTCGCCAGGAGACAATATCGCCCGCTTGATAGTCACTGGGGTTCTTGCTGGTGGGGCGTGTTTTATCATGGCGGCTAAACCAGGTTTCCAGATTAGGCACCCGACGGTGATCGATGTTGCTGTCCGGGCGCTTTAACTTCCATTTTTGCGGGTACTCAGCAAAATT  >  NC_000913/4137105‑4137360                                                                                                                             |                                                                                                                                    cgcCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGCGAAGCCATCTGAAACCACCCCAATATGTGCCAGCCCGTTATCCAGTCGCCAGGAGACAATATCGCCCGCTTGATAGTCGCTGGGGTTCTTGCt                                                                                                                       >  2:731203/1‑139 (MQ=255)   ccTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGCGAAGCCATCTGAAACCACCCCAATATGTGCCAGCCCGTTATCCAGTCGCCAGGAGACAATATCGCCCGCTTGATAGTCGCTGGGGTTCTTGCtgg                                                                                                                     <  1:956142/139‑1 (MQ=255)        gcCAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGCGAAGCCATCTGAAACCACCCCAATATGTGCCAGCCCGTTATCCAGTCGCCAGGAGACAATATCGCCCGCTTGATAGTCGCTGGGGTTCTTGCTGGTgggg                                                                                                                <  1:1005494/139‑1 (MQ=255)         ccAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGCGAAGCCATCTGAAACCACCCCAATATGTGCCAGCCCGTTATCCAGTCGCCAGGAGACAATATCGCCCGCTTGATAGTCGCTGGGGTTCTTGCTGGTGGGGc                                                                                                               <  1:263010/139‑1 (MQ=255)         ccAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGCGAAGCCATCTGAAACCACCCCAATATGTGCCAGCCCGTTATCCAGTCGCCAGGAGACAATATCGCCCGCTTGATAGTCGCTGGGGTTCTTGCTGGTGGGGc                                                                                                               <  2:30499/139‑1 (MQ=255)                       cACCAGCGGCGTACCGTCGCGGGCGAAGCCATCTGAAACCACCCCAATATGTGCCAGCCCGTTATCCAGTCGCCAGGAGACAATATCGCCCGCTTGATAGTCGCTGGGGTTCTTGCTGGTGGGGCGTGTTTTATCATgg                                                                                                 >  2:314030/1‑139 (MQ=255)                           aGCGGCGTACCGTCGCGGGCGAAGCCATCTGAAACCACCCCAATATGTGCCAGCCCGTTATCCAGTCGCCAGGAGACAATATCGCCCGCTTGATAGTCGCTGGGGTTCTTGCTGGTGGGGCGTGTTTTATCATggcggc                                                                                             >  2:80004/1‑139 (MQ=255)                            gcggcgTACCGTCGCGGGCGAAGCCATCTGAAACCACCCCAATATGTGCCAGCCCGTTATCCAGTCGCCAGGAGACAATATCGCCCGCTTGATAGTCGCTGGGGTTCTTGCTGGTGGGGc                                                                                                               >  1:494617/1‑120 (MQ=255)                            gcggcgTACCGTCGCGGGCGAAGCCATCTGAAACCACCCCAATATGTGCCAGCCCGTTATCCAGTCGCCAGGAGACAATATCGCCCGCTTGATAGTCGCTGGGGTTCTTGCTGGTGGGGc                                                                                                               <  2:494617/120‑1 (MQ=255)                                cgTACCGTCGCGGGCGAAGCCATCTGAAACCACCCCAATATGTGCCAGCCCGTTATCCAGTCGCCAGGAGACAATATCGCCCGCTTGATAGTCGCTGGGGTTCTTGCTGGTGGGGc                                                                                                               >  2:172626/1‑116 (MQ=255)                                cgTACCGTCGCGGGCGAAGCCATCTGAAACCACCCCAATATGTGCCAGCCCGTTATCCAGTCGCCAGGAGACAATATCGCCCGCTTGATAGTCGCTGGGGTTCTTGCTGGTGGGGc                                                                                                               <  1:172626/116‑1 (MQ=255)                                    ccGTCGCGGGCGAAGCCATCTGAAACCACCCCAATATGTGCCAGCCCGTTATCCAGTCGCCAGGAGACAATATCGCCCGCTTGATAGTCGCTGGGGTTCTTGCTGGTGGGGCGTGTTTTATCATGGCGGCTAAACCAgg                                                                                    >  1:997980/1‑139 (MQ=255)                                                       cTGAAACCACCCCAATATGTGCCAGCCCGTTATCCAGTCGCCAGGAGACAATATCGCCCGCTTGATAGTCGCTGGGGTTCTTGCTGGTGGGGCGTGTTTTATCATGGCGGCTAAACCa                                                                                      <  2:891156/118‑1 (MQ=255)                                                       cTGAAACCACCCCAATATGTGCCAGCCCGTTATCCAGTCGCCAGGAGACAATATCGCCCGCTTGATAGTCGCTGGGGTTCTTGCTGGTGGGGCGTGTTTTATCATGGCGGCTAAACCa                                                                                      >  1:891156/1‑118 (MQ=255)                                                                ccccAATATGTGCCAGCCCGTTATCCAGTCGCCAGGAGACAATATCGCCCGCTTGATAGTCGCTGGGGTTCTTGCTGGTGGGGCGTGTTTTATCATGGCGGCTAAACCAGGTTTCCAGATTAGGCACCCGACGGTGATc                                                        >  1:979818/1‑139 (MQ=255)                                                                        tgtgACAGCCCGTTATCCAGTCGCCAGGAGACAATATCGCCCGCTTGATAGTCGCTGGGGTTCTTGCTGGTGGGGCGTGTTTTATCATGGCGGCTAAACCAGGTTTCCAGATTAGGCGCCCGACGGTGATCGATGTTGc                                                >  1:587113/1‑139 (MQ=255)                                                                          tgCCAGCCCGTTATCCAGTCGCCAGGAGACAATATCGCCCGCTTGATAGTCGCTGGGGTTCTTGCTGGTGGGGCGTGTTTTATCATg                                                                                                  <  1:12102/87‑1 (MQ=255)                                                                          tgCCAGCCCGTTATCCAGTCGCCAGGAGACAATATCGCCCGCTTGATAGTCGCTGGGGTTCTTGCTGGTGGGGCGTGTTTTATCATg                                                                                                  >  2:12102/1‑87 (MQ=255)                                                                          tgCCAGCCCGTTATCCAGTCGCCAGGAGACAATATCGCCCGCTTGATAGTCGCTGGGGTTCTTGCTGGTGGGGCGTGTTTTATCATGGCGGCTAAACCAGGTTTCCAGATTAGGCACCCGACGGTGATCGATGTTGCTg                                              >  2:898279/1‑139 (MQ=255)                                                                           gCCAGCCCGTTATCCAGTCGCCAGGAGACAATATCGCCCGCTTGATAGTCGCTGGGGTTCTTGCTGGTGGGGCGTGTTTTATCATGGCGGCTAAACCAGGTTTCCAGATTAGGCACCCGACGGTGATCGATGTTGCTGt                                             >  1:388260/1‑139 (MQ=255)                                                                               gCCCGTTATCCAGTCGCCAGGAGACAATATCGCCCGCTTGATAGTCGCTGGGGTTCTTGCTGGTGGGGCGTGTTTTATCATGGCGGCTAAACCAGGTTTCCAGATTAGGCACCCGACGGTGAt                                                         <  2:388933/123‑1 (MQ=255)                                                                               gCCCGTTATCCAGTCGCCAGGAGACAATATCGCCCGCTTGATAGTCGCTGGGGTTCTTGCTGGTGGGGCGTGTTTTATCATGGCGGCTAAACCAGGTTTCCAGATTAGGCACCCGACGGTGAt                                                         >  1:388933/1‑123 (MQ=255)                                                                                cccGTTATCCAGTCGCCAGGAGACAATATCGCCCGCTTGATAGTCGCTGGGGTTCTTGCTGGTGGGGc                                                                                                               >  1:525250/1‑68 (MQ=255)                                                                                cccGTTATCCAGTCGCCAGGAGACAATATCGCCCGCTTGATAGTCGCTGGGGTTCTTGCTGGTGGGGc                                                                                                               <  2:525250/68‑1 (MQ=255)                                                                                cccGTTATCCAGTCGCCAGGAGACAATATCGCCCGCTTGATAGTCGCTGGGGTTCTTGCTGGTGGGGCGTGTTTTATCATGGCGGCTAAACCAg                                                                                     >  1:593355/1‑94 (MQ=255)                                                                                cccGTTATCCAGTCGCCAGGAGACAATATCGCCCGCTTGATAGTCGCTGGGGTTCTTGCTGGTGGGGCGTGTTTTATCATGGCGGCTAAACCAg                                                                                     <  2:593355/94‑1 (MQ=255)                                                                                                 aGGAGACAATATCGCCCGCTTGATAGTCGCTGGGGTTCTTGCTGGTgggg                                                                                                                <  2:374109/50‑1 (MQ=255)                                                                                                 aGGAGACAATATCGCCCGCTTGATAGTCGCTGGGGTTCTTGCTGGTgggg                                                                                                                >  1:374109/1‑50 (MQ=255)                                                                                                  ggAGACAATATCGCCCGCTTGATAGTCGCTGGGGTTCTTGCTGGTGGGGCGTGTTTTATCATGGCGGCTAAACCAGGTTTCCAGATTAGGCACCCGACGGTGATCGATGTTGCTGTCCGGGCGCTTTAACTTCCAtttt                      <  1:314030/139‑1 (MQ=255)                                                                                                          tatCGCCCGCTTGATAGTCGCTGGGGTTCTTGCTGGTGGGGCGTGTTTTATCATGGCGGCTAAACCAGGTTTCCAGATTAGGCACCCGAc                                                               <  1:926861/90‑1 (MQ=255)                                                                                                          tatCGCCCGCTTGATAGTCGCTGGGGTTCTTGCTGGTGGGGCGTGTTTTATCATGGCGGCTAAACCAGGTTTCCAGATTAGGCACCCGAc                                                               >  2:926861/1‑90 (MQ=255)                                                                                                          tatCGCCCGCTTGATAGTCGCTGGGGTTCTTGCTGGTGGGGCGTGTTTTATCATGGCGGCTAAACCAGGTTTCCAGATTAGGCACCCGACGGTGATCGATGTTGCTGTCCGGGCGCTTTAACTTCCATTTTTGCGGGTa              >  2:271036/1‑139 (MQ=255)                                                                                                                      gATAGTCGCTGGGGTTCTTGCTGGTGGGGCGTGTTTTATCATGGCGGCTAAACCAGGTTTCCAGATTAGGCACCCGACGGTGATCGATGTTGCTGTCCGGGCGCTTTAACTTCCATTTTTGCGGGTACTCAGCAAAAtt  <  2:997980/139‑1 (MQ=255)                                                                                                                             |                                                                                                                                    CGCCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGCGAAGCCATCTGAAACCACCCCAATATGTGCCAGCCCGTTATCCAGTCGCCAGGAGACAATATCGCCCGCTTGATAGTCACTGGGGTTCTTGCTGGTGGGGCGTGTTTTATCATGGCGGCTAAACCAGGTTTCCAGATTAGGCACCCGACGGTGATCGATGTTGCTGTCCGGGCGCTTTAACTTCCATTTTTGCGGGTACTCAGCAAAATT  >  NC_000913/4137105‑4137360

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑