Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	37,903	C→T	D405N (GAC→AAC)	caiB ←	crotonobetainyl CoA:carnitine CoA transferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	37,903	0	C	T	100.0%	114.9 / NA	36	D405N (GAC→AAC)	caiB	crotonobetainyl CoA:carnitine CoA transferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (20/16);  total (20/16)

TCCGCAAGATCGTCCCACATTTGACGTAGATGTTGTCCGCCAATGATATCCATTGCACCTCTATCCATTTTTGTTCGTTTGTTATTGGGCGGGCGCTAGTCAGGCAAGCCGACTGACGCCACGCGTTTAGTCCTCAACTTTGGCCAGACCTTTGCTGACCAACTCCTGAATGTCGTTTTCGCTGTAGCCGATATTTTTCAAAATGGCAGCCGTGTCCATGCCATGTGAGGGCATTCCGCGCCAGATTTGTCCGGGG  >  NC_000913/37772‑38027                                                                                                                                    |                                                                                                                             tCCGCAAGATCGTCCCACATTTGACGTAGATGTTGTCCGCCAATGATATCCATTGCACCTCTATCCATTTTTGTTCGTTTGTTATTGGGCGGGCGCTAGTCAGGCAAGCCGACTGACGCCACGCGTTTAGTTCTCAACt                                                                                                                       <  2:74755/139‑1 (MQ=255) tCCGCAAGATCGTCCCACATTTGACGTAGATGTTGTCCGCCAATGATATCCATTGCACCTCTATCCATTTTTGTTCGTTTGTTATTGGGCGGGCGCTAGTCAGGCAAGCCGACTGACGCCACGCGTTTAGTTCTCAACt                                                                                                                       <  2:464939/139‑1 (MQ=255)           cGTCCCACATTTGACGTAGATGTTGTCCGCCAATGATATCCATTGCACCTCTATCCATTTTTGTTCGTTTGTTATTGGGCGGGCGCTAGTCAGGCAAGCCGACTGACGCCACGCGTTTAGTTCTCAACTTTGGCCAGAc                                                                                                             >  2:569531/1‑139 (MQ=255)           cGTCCCACATTTGACGTAGATGTTGTCCGCCAATGATATCCATTGCACCTCTATCCATTTTTGTTCGTTTGTTATTGGGCGGGCGCTAGTCAGGCAAGCCGACTGACGCCACGCGTTTAGTTCTCAACTTTGGCCAGAc                                                                                                             <  2:247506/139‑1 (MQ=255)                   aTTTGACGTAGATGTTGTCCGCCAATGATATCCATTGCACCTCTATCCATTTTTGTTCGTTTGTTATTGGGCGGGCGCTAGTCAGGCAAGCCGACTGACGCCACGCGTTTAGTTCTCAACTTTGGCCAGACCTTTGCTg                                                                                                     >  1:879868/1‑139 (MQ=255)                        aCGTAGATGTTGTCCGCCAATGATATCCATTGCACCTCTATCCATTTTTGTTCGTTTGTTATTGGGCGGGCGCTAGTCAGGCAAGCCGACTGACGCCACGCGTTTAGTTCTCAACt                                                                                                                       <  1:145638/116‑1 (MQ=255)                        aCGTAGATGTTGTCCGCCAATGATATCCATTGCACCTCTATCCATTTTTGTTCGTTTGTTATTGGGCGGGCGCTAGTCAGGCAAGCCGACTGACGCCACGCGTTTAGTTCTCAACt                                                                                                                       >  2:145638/1‑116 (MQ=255)                                   gtCCGCCAATGATATCCATTGCACCTCTATCCATTTTTGTTCGTTTGTTATTGGGCGGGCGCTAGTCAGGCAAGCCGACTGACGCCACGCGTTTAGTTCTCAACTTTGGCCAGACCTTTGCTGACCAACTCCTGAATGt                                                                                     >  1:320691/1‑139 (MQ=255)                                      cGCCAATGATATCCATTGCACCTCTATCCATTTTTGTTCGTTTGTTATTGGGCGGGCGCTAGTCAGGCAAGCCGACTGACGCCACGCGTTTAGTTCTCAACTTTGGCCAGACCTTTGCTGACCAACTCCTGAATGTCGt                                                                                  >  2:299195/1‑139 (MQ=255)                                           aTGATATCCATTGCACCTCTATCCATTTTTGTTCGTTTGTTATTGGGCGGGCGCTAGTCAGGCAAGCCGACTGACGCCACGCGTTTAGTTCTCAACTTTGGCCAGACCTTTGCTGACCAACTCCTGAATGTCGTTTTCg                                                                             <  2:879868/139‑1 (MQ=255)                                                      tGCACCTCTATCCATTTTTGTTCGTTTGTTATTGGGCGGGCGCTAGTCAGGCAAGCCGACTGACGCCACGCGTTTAGTTCt                                                                                                                            >  1:600249/1‑81 (MQ=255)                                                      tGCACCTCTATCCATTTTTGTTCGTTTGTTATTGGGCGGGCGCTAGTCAGGCAAGCCGACTGACGCCACGCGTTTAGTTCTCAACTTTGGCCAGACCTTTg                                                                                                        <  1:763892/101‑1 (MQ=255)                                                      tGCACCTCTATCCATTTTTGTTCGTTTGTTATTGGGCGGGCGCTAGTCAGGCAAGCCGACTGACGCCACGCGTTTAGTTCTCAACTTTGGCCAGACCTTTg                                                                                                        >  2:763892/1‑101 (MQ=255)                                                      tGCACCTCTATCCATTTTTGTTCGTTTGTTATTGGGCGGGCGCTAGTCAGGCAAGCCGACTGACGCCACGCGTTTAGTTCTCAACTTTGGCCAGACCTTTGCTGACCAACTCCTGAATGTCGTTTTCGCTGTAGCCGat                                                                  >  2:725317/1‑139 (MQ=255)                                                      tGCACCTCTATCCATTTTTGTTCGTTTGTTATTGGGCGGGCGCTAGTCAGGCAAGCCGACTGACGCCACGCGTTTAGTTCTCAACTTTGGCCAGACCTTTGCTGACCAACTCCTGAATGTCGTTTTCGCTGTAGCCGat                                                                  >  1:486650/1‑139 (MQ=255)                                                       gCACCTCTATCCATTTTTGTTCGTTTGTTATTGGGCGGGCGCTAGTCAGGCAAGCCGACTGACGCCACGCGTTTAGTTCTCAACTTTGGCCAGACCTTTGCTGACCAACTCCTGAATGTCGTTTTCGCt                                                                           <  2:690211/129‑1 (MQ=255)                                                       gCACCTCTATCCATTTTTGTTCGTTTGTTATTGGGCGGGCGCTAGTCAGGCAAGCCGACTGACGCCACGCGTTTAGTTCTCAACTTTGGCCAGACCTTTGCTGACCAACTCCTGAATGTCGTTTTCGCt                                                                           >  1:690211/1‑129 (MQ=255)                                                             ctATCCATTTTTGTTCGTTTGTTATTGGGCGGGCGCTAGTCAGGCAAGCCGACTGACGCCACGCGTTTAGTTCt                                                                                                                            <  2:534221/74‑1 (MQ=255)                                                             ctATCCATTTTTGTTCGTTTGTTATTGGGCGGGCGCTAGTCAGGCAAGCCGACTGACGCCACGCGTTTAGTTCTCAACTTTGGCCAGACCTTTGCTGACCAACTCCTGAATGTCGTTTTCGCTGTAGCCGATATTTTTc                                                           <  1:665963/139‑1 (MQ=255)                                                             ctATCCATTTTTGTTCGTTTGTTATTGGGCGGGCGCTAGTCAGGCAAGCCGACTGACGCCACGCGTTTAGTTCTCAACTTTGGCCAGACCTTTGCTGACCAACTCCTGAATGTCGTTTTCGCTGTAGCCGATATTTTTc                                                           >  2:665963/1‑139 (MQ=255)                                                             ctATCCATTTTTGTTCGTTTGTTATTGGGCGGGCGCTAGTCAGGCAAGCCGACTGACGCCACGCGTTTAGTTCTCAACTTTGGCCAGACCTTTGCTGACCAACTCCTGAATGTCGTTTTCGCTGTAGCCGATATTTTTc                                                           >  1:171037/1‑139 (MQ=255)                                                                tCCATTTTTGTTCGTTTGTTATTGGGCGGGCGCTAGTCAGGCAAGCCGACTGACGCCACGCGTTTAGTTCTCAACTTTGGCCAGACCTTTGCTGACCAACTCCTGAATGTCGTTTTCGc                                                                            >  1:726322/1‑119 (MQ=255)                                                                tCCATTTTTGTTCGTTTGTTATTGGGCGGGCGCTAGTCAGGCAAGCCGACTGACGCCACGCGTTTAGTTCTCAACTTTGGCCAGACCTTTGCTGACCAACTCCTGAATGTCGTTTTCGc                                                                            <  2:726322/119‑1 (MQ=255)                                                                        tGTTCGTTTGTTATTGGGCGGGCGCTAGTCAGGCAAGCCGACTGACGCCACGCGTTTAGTTCTCAACTTTGGCCAGACCTTTGCTGACCAACTCCTGAATGTCGTTTTCGCTGTAGCCGATATTTTTCAAAATGGCAGc                                                <  2:486650/139‑1 (MQ=255)                                                                        tGTTCGTTTGTTATTGGGCGGGCGCTAGTCAGGCAAGCCGACTGACGCCACGCGTTTAGTTCTCAACTTTGGCCAGACCTTTGCTGACCAACTCCTGAATGTCGTTTTCGCTGTAGCCGATATTTTTCAAAATGGCAGc                                                <  1:678049/139‑1 (MQ=255)                                                                          ttCGTTTGTTATTGGGCGGGCGCTAGTCAGGCAAGCCGACTGACGCCACGCGTTTAGTTCTCAACTTTGGCCAGACCTTTGCTGACCAACTCCTGAATGTCGTTTTCGCTGTAGCCGATATTTTTCAAAATGGCAGCCg                                              >  2:178567/1‑139 (MQ=255)                                                                          ttCGTTTGTTATTGGGCGGGCGCTAGTCAGGCAAGCCGACTGACGCCACGCGTTTAGTTCTCAACTTTGGCCAGACCTTTGCTGACCAACTCCTGAATGTCGTTTTCGCTGTAGCCGATATTTTTCAAAATGGCAGCCg                                              >  1:123016/1‑139 (MQ=255)                                                                                        ggcgggcgCTAGTCAGGCAAGCCGACTGACGCCACGCGTTTAGTTCTCAACTTTGGCCAGACCTTTGCTGACCAACTCCTGAATGTCGTTTTCGCTGTAGCCGATATTTTTCAAAATGGCAGCCGTGTCCATGCCAtgt                                >  2:321591/1‑139 (MQ=255)                                                                                         gcgggcgCTAGTCAGGCAAGCCGACTGACGCCACGCGTTTAGTTCTCAACTTTGGCCAGACCTTTGCTGACCAACTCCTGAATGTCGTTTTCGCTGTAGCCGATATTTTTCAAAATGGCAGCCGTGTCCATGCCAtgtg                               >  2:215193/1‑139 (MQ=255)                                                                                                  aGTCAGGCAAGCCGACTGACGCCACGCGTTTAGTTCTCAACTTTGGCCAGACCTTTGCTGACCAACTCCTGAATGTCGTTTTCGCTGTAGCCGATATTTTTCAAAATGGCAGCCGTGTCCATGCCATGTGAGGGCATTc                      <  1:178567/139‑1 (MQ=255)                                                                                                        gCAAGCCGACTGACGCCACGCGTTTAGTTCTCAACTTTGGCCAGACCTTTGCTGACCAACTCCTGAATGTCGTTTTCGCTGTAGCCGATATTTTTCAAAATGGCAGCCGTGTCCATGCCATGTGAGGGCATTCCGCGcc                >  1:332556/1‑139 (MQ=255)                                                                                                             ccGACTGACGCCACGCGTTTAGTTCTCAACTTTGGCCAGACCTTTGCTGACCAACTCCTGAATGTCGTTTTCGCTGTAGCCGATATTTTTCAAAATGGCAGcc                                               <  2:944096/103‑1 (MQ=255)                                                                                                             ccGACTGACGCCACGCGTTTAGTTCTCAACTTTGGCCAGACCTTTGCTGACCAACTCCTGAATGTCGTTTTCGCTGTAGCCGATATTTTTCAAAATGGCAGcc                                               >  1:944096/1‑103 (MQ=255)                                                                                                                 cTGACGCCACGCGTTTAGTTCTCAACTTTGGCCAGACCTTTGCTGACCAACTCCTGAATGTCGTTTTCGCTGTAGCCGATATTTTTCAAAATGGCAGCCGTGTCCATGCCATGTGAGGGCATTCCGCGCCAGATTTGTc       <  2:829460/139‑1 (MQ=255)                                                                                                                  tGACGCCACGCGTTTAGTTCTCAACTTTGGCCAGACCTTTGCTGACCAACTCCTGAATGTCGTTTTCGCTGTAGCCGATATTTTTCAAAATGGCAGCCGTGTCCATGCCATGTGAGGGCATTCCGCGCCAGATTTGTcc      <  2:332556/139‑1 (MQ=255)                                                                                                                      gCCACGCGTTTAGTTCTCAACTTTGGCCAGACCTTTGCTGACCAACTCCTGAATGTCGTTTTCGCTGTAGCCGATATTTTTCAAAATGGCAGCCGTGTCCATGCCATGTGAGGGCATTCCGCGCCAGATTTGTCCgggg  >  2:55190/1‑139 (MQ=255)                                                                                                                                    |                                                                                                                             TCCGCAAGATCGTCCCACATTTGACGTAGATGTTGTCCGCCAATGATATCCATTGCACCTCTATCCATTTTTGTTCGTTTGTTATTGGGCGGGCGCTAGTCAGGCAAGCCGACTGACGCCACGCGTTTAGTCCTCAACTTTGGCCAGACCTTTGCTGACCAACTCCTGAATGTCGTTTTCGCTGTAGCCGATATTTTTCAAAATGGCAGCCGTGTCCATGCCATGTGAGGGCATTCCGCGCCAGATTTGTCCGGGG  >  NC_000913/37772‑38027

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑