Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,353,983	T→C	K245K (AAA→AAG)	lysU ←	lysine tRNA synthetase, inducible

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,353,983	0	T	C	100.0%	215.1 / NA	60	K245K (AAA→AAG)	lysU	lysine tRNA synthetase, inducible
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (29/31);  total (29/31)

TCCGCATACGCCATGTAGAGTTCCATCATTGTGAACTCAGGATTATGGCGAACAGAAATACCTTCATTACGGAAGTTACGGTTGATTTCGAATACCCGTTCAAAACCGCCTACAACCAGACGTTTCAGATACAGCTCCGGCGCGATACGCAGATACATATCTAAATCCAGAGCATTATGATGGGTAATAAACGGGCGAGCAGATGCCCCACCTGGAATTACCTGCATCATCGGGGTTTCTAC  >  NC_000913/4353861‑4354102                                                                                                                           |                                                                                                                        tCCGCATACGCCATGTAGAGTTCCATCATTGTGAACTCAGGATTATGGTGAACAGAAATACCTTCATTACGGAAGTTACGGTTGATTTCGAATACCCGTTCAAAACCGCCTACAACCAGACGCTTCAGATACAGCTCCg                                                                                                         >  2:824086/1‑139 (MQ=255)     cATACGCCATGTAGAGTTCCATCATTGTGAACTCAGGATTATGGCGAACAGAAATACCTTCATTACGGAAGTTACGGTTGATTTCGAATACCCGTTCAAAACCGCCTACAACCAGACGCTTCAGATACAGCTCCGgcgc                                                                                                     >  2:994176/1‑139 (MQ=255)      aTACGCCATGTAGAGTTCCATCATTGTGAACTCAGGATTATGGCGAACAGAAATACCTTCATTACGGAAGTTACGGTTGATTTCGAATACCCGTTCAAAACCGCCTACAACCAGACGCTTCAGATACAGCTCCGGcgcg                                                                                                    <  2:725856/139‑1 (MQ=255)      aTACGCCATGTAGAGTTCCATCATTGTGAACTCAGGATTATGGCGAACAGAAATACCTTCATTACGGAAGTTACGGTTGATTTCGAATACCCGTTCAAAACCGCCTACAACCAGACGCTTCAGATACAGCTCCGGcgcg                                                                                                    <  1:328355/139‑1 (MQ=255)       tACGCCATGTAGAGTTCCATCATTGTGAACTCAGGATTATGGCGAACAGAAATACCTTCATTACGGAAGTTACGGTTGATTTCGAATACCCGTTCAAAACCGCCTACAACCAGACGCTTCAGATACAGCTCCGGCGCGa                                                                                                   <  2:816984/139‑1 (MQ=255)           ccATGTAGAGTTCCATCATTGTGAACTCAGGATTATGGCGAACAGAAATACCTTCATTACGGAAGTTACGGTTGATTTCGAATACCCGTTCAAAACCGCCTACAACCAGACGCTTCAGATACAGCTCCGGCGCGATACg                                                                                               <  1:994176/139‑1 (MQ=255)                   agTTCCATCATTGTGAACTCAGGATTATGGCGAACAGAAATACCTTCATTACGGAAGTTACGGTTGATTTCGAATACCCGTTCAAAACCGCCTACAACCAGACGCTTCAGATACAGCTCCGGCGCGa                                                                                                   >  2:959178/1‑127 (MQ=255)                   agTTCCATCATTGTGAACTCAGGATTATGGCGAACAGAAATACCTTCATTACGGAAGTTACGGTTGATTTCGAATACCCGTTCAAAACCGCCTACAACCAGACGCTTCAGATACAGCTCCGGCGCGa                                                                                                   <  1:959178/127‑1 (MQ=255)                   agTTCCATCATTGTGAACTCAGGATTATGGCGAACAGAAATACCTTCATTACGGAAGTTACGGTTGATTTCGAATACCCGTTCAAAACCGCCTACAACCAGACGCTTCAGATACAGCTCCGGCGCGATACGCAGATACa                                                                                       >  1:325754/1‑139 (MQ=255)                    gTTCCATCATTGTGAACTCAGGATTATGGCGAACAGAAATACCTTCATTACGGAAGTTACGGTTGATTTCGAATACCCGTTCAAAACCGCCTACAACCAGACGCTTCa                                                                                                                     >  2:21318/1‑108 (MQ=255)                    gTTCCATCATTGTGAACTCAGGATTATGGCGAACAGAAATACCTTCATTACGGAAGTTACGGTTGATTTCGAATACCCGTTCAAAACCGCCTACAACCAGACGCTTCa                                                                                                                     <  1:21318/108‑1 (MQ=255)                     ttCCATCATTGTGAACTCAGGATTATGGCGAACAGAAATACCTTCATTACGGAAGTTACGGTTGATTTCGAATACCCGTTCAAAACCGCCTACAACCAGACGCTTCAGATACAGCTCCGGCGCGATACGCa                                                                                             <  2:828748/131‑1 (MQ=255)                     ttCCATCATTGTGAACTCAGGATTATGGCGAACAGAAATACCTTCATTACGGAAGTTACGGTTGATTTCGAATACCCGTTCAAAACCGCCTACAACCAGACGCTTCAGATACAGCTCCGGCGCGATACGCa                                                                                             >  1:828748/1‑131 (MQ=255)                             ttGTGAACTCAGGATTATGGCGAACAGAAATACCTTCATTACGGAAGTTACGGTTGATTTCGAATACCCGTTCAAAACCGCCTACAACCAGACGCTTCAGATACAGCTCCGGcgcg                                                                                                    >  2:547941/1‑116 (MQ=255)                             ttGTGAACTCAGGATTATGGCGAACAGAAATACCTTCATTACGGAAGTTACGGTTGATTTCGAATACCCGTTCAAAACCGCCTACAACCAGACGCTTCAGATACAGCTCCGGcgcg                                                                                                    <  1:547941/116‑1 (MQ=255)                                 gAACTCAGGATTATGGCGAACAGAAATACCTTCATTACGGAAGTTACGGTTGATTTCGAATACCCGTTCAAAACCGCCTACAACCAGACGCTTCAGATACAGCTCCGGCGCGATACGCAGATACATATCTAAATCCaga                                                                         <  2:325754/139‑1 (MQ=255)                                  aaCTCAGGATTATGGCGAACAGAAATACCTTCATTACGGAAGTTACGGTTGATTTCGAATACCCGTTCAAAAACGCCTACAACCAGACGCTTCAGATACAGCTCCGGCGCGATACGCAGAAACATATCTAAATCCagag                                                                        >  2:280627/1‑139 (MQ=255)                                     tCAGGATTATGGCGAACAGAAATACCTTCATTACGGAAGTTACGGTTGATTTCGAATACCCGTTCAAAACCGCCTACAACCAGACGCTTCAGATa                                                                                                                 >  2:900006/1‑95 (MQ=255)                                     tCAGGATTATGGCGAACAGAAATACCTTCATTACGGAAGTTACGGTTGATTTCGAATACCCGTTCAAAACCGCCTACAACCAGACGCTTCAGATa                                                                                                                 <  1:900006/95‑1 (MQ=255)                                     tCAGGATTATGGCGAACAGAAATACCTTCATTACGGAAGTTACGGTTGATTTCGAATACCCGTTCAAAACCGCCTACAACCAGACGCTTCAGATACAGCTCCGGCGCGATACGCAGATACATATCTAAATCCAGAGCAt                                                                     >  2:81241/1‑139 (MQ=255)                                       aGGATTATGGCGAACAGAAATACCTTCATTACGGAAGTTACGGTTGATTTCGCATACCCGTTCAAAACCGCCTACAACCAGACGCTTCAGATACAGCTCCGGCGCGATACGCAGATACATATCTAAATCCAGAGCATTa                                                                   >  1:557998/1‑139 (MQ=255)                                       aGGATTATGGCGAACAGAAATACCTTCATTACGGAAGTTACGGTTGATTTCGAATACCCGTTCAAAACCGCCTACAACCAGACGCTTCAGATACAGCTCCGGCGCGAt                                                                                                  >  1:244237/1‑108 (MQ=255)                                       aGGATTATGGCGAACAGAAATACCTTCATTACGGAAGTTACGGTTGATTTCGAATACCCGTTCAAAACCGCCTACAACCAGACGCTTCAGATACAGCTCCGGCGCGAt                                                                                                  <  2:244237/108‑1 (MQ=255)                                       aGGATTATGGCGAACAGAAATACCTTCATTACGGAAGTTACGGTTGATTTCGAATACCCGTTCAAAACCGCCTACAACCAGACGCTTCAGATACAGCTCCGGCGCGATACGCAGATACATATCTAAATCCAGAGCATTa                                                                   >  1:252841/1‑139 (MQ=255)                                         gATTATGGCGAACAGAAATACCTTCATTACGGAAGTTACGGTTGATTTCGAATACCCGTTCAAAACCGCCTACAACCAGACGCTTCAGATACAGCTCCGGCGCGATACg                                                                                               >  1:806133/1‑109 (MQ=255)                                         gATTATGGCGAACAGAAATACCTTCATTACGGAAGTTACGGTTGATTTCGAATACCCGTTCAAAACCGCCTACAACCAGACGCTTCAGATACAGCTCCGGCGCGATACg                                                                                               <  2:806133/109‑1 (MQ=255)                                         gATTATGGCGAACAGAAACACCTTCATTACGGAAGTTACGGTTGATTTCGAATACCCGTTCAAAACCGCCTACAACCAGACGCTTCAGATACAGCTCCGGCGCGATACGCAGATACATATCTAAATCCAGAGCATTatg                                                                 <  2:679334/139‑1 (MQ=255)                                          aTTATGGCGAACAGAAATACCTTCATTACGGAAGTTACGGTTGATTTCGAATACCCGTTCAAAACCGCCTACAACCAGACGCTTCAGATACAGCTCCGGCGCGATACGCAGATACATATCTAAATCCAGAGCATTatga                                                                <  2:557998/139‑1 (MQ=255)                                           ttATGGCGAACAGAAATACCTTCATTACGGAAGTTACGGTTGATTTCGAATACCCGTTCAAAACCGCCTACAACCAGACGCTTCAGATACAGCTCCGGCGCGATACGCAGATACATATCTAAATCCAGAGCATTatgat                                                               <  2:252841/139‑1 (MQ=255)                                              tGGCGAACAGAAATACCTTCATTACGGAAGTTACGGTTGATTTCGAATACCCGTTCAAAACCGCCTACAACCAGACGCTTCAGATACAGCTCCGGCGCGATACGCAGATACa                                                                                       <  1:112841/112‑1 (MQ=255)                                              tGGCGAACAGAAATACCTTCATTACGGAAGTTACGGTTGATTTCGAATACCCGTTCAAAACCGCCTACAACCAGACGCTTCAGATACAGCTCCGGCGCGATACGCAGATACa                                                                                       >  2:112841/1‑112 (MQ=255)                                                     cAGAAATACCTTCATTACGGAAGTTACGGTTGATTTCGAATACCCGTTCAAAACCGCCTACAACCAGACGCTTCAGATACAGCTCCGGCGCGATACGCAGATACATATCTAAATCCAGAGCATTATGATGGGTAATaaa                                                     <  2:743241/139‑1 (MQ=255)                                                              cTTCATTACGGAAGTTACGGTTGATTTCGAATACCCGTTCAAAACCGCCTACAACCAGACGCTTCAGATACAGCTCCGGCGCGATACGCAGATACATATCTAAATCCa                                                                           >  1:599203/1‑108 (MQ=255)                                                              cTTCATTACGGAAGTTACGGTTGATTTCGAATACCCGTTCAAAACCGCCTACAACCAGACGCTTCAGATACAGCTCCGGCGCGATACGCAGATACATATCTAAATCCa                                                                           <  2:599203/108‑1 (MQ=255)                                                              cTTCATTACGGAAGTTACGGTTGATTTCGAATACCCGTTCAAAACCGCCTACAACCAGACGCTTCAGATACAGCTCCGGCGCGATACGCAGATACATATCTAAATCCAGAGCATTatgatg                                                              >  2:167686/1‑121 (MQ=255)                                                              cTTCATTACGGAAGTTACGGTTGATTTCGAATACCCGTTCAAAACCGCCTACAACCAGACGCTTCAGATACAGCTCCGGCGCGATACGCAGATACATATCTAAATCCAGAGCATTatgatg                                                              <  1:167686/121‑1 (MQ=255)                                                                  aTTACGGAAGTTACGGTTGATTTCGAATACCCGTTCAAAACTGCCTACAACAAGACGCTTCAGATACAGCTCCGGCGCGATACGCAGATACa                                                                                       >  1:623922/1‑92 (MQ=255)                                                                  aTTACGGAAGTTACGGTTGATTTCGAATACCCGTTCAAAACCGCCTACAACCAGACGCTTCAGATa                                                                                                                 <  2:709130/66‑1 (MQ=255)                                                                  aTTACGGAAGTTACGGTTGATTTCGAATACCCGTTCAAAACCGCCTACAACCAGACGCTTCAGATa                                                                                                                 >  1:709130/1‑66 (MQ=255)                                                                  aTTACGGAAGTTACGGTTGATTTCGAATACCCGTTCAAAACCGCCTACAACCAGACGCTTCAGATACAGCTCCGGCGCGATACGCAGATACa                                                                                       <  1:868077/92‑1 (MQ=255)                                                                  aTTACGGAAGTTACGGTTGATTTCGAATACCCGTTCAAAACCGCCTACAACCAGACGCTTCAGATACAGCTCCGGCGCGATACGCAGATACa                                                                                       >  2:868077/1‑92 (MQ=255)                                                                  aTTACGGAAGTTACCGTTGATTTCGAATACCCGTTCAAAACCGCCTACAACCAGACGCTTCAGATACAGCTCCGGCGCGATACGCAGATACa                                                                                       <  2:623922/92‑1 (MQ=255)                                                                    tACGGAAGTTACGGTTGATTTCGAATACCCGTTCAAAACCGCCTACAACCAGACGCTTCAGATACAGCTCCGGCGCGATACGCAGATACATATCTAAATCCa                                                                           >  2:62336/1‑102 (MQ=255)                                                                    tACGGAAGTTACGGTTGATTTCGAATACCCGTTCAAAACCGCCTACAACCAGACGCTTCAGATACAGCTCCGGCGCGATACGCAGATACATATCTAAATCCa                                                                           <  1:62336/102‑1 (MQ=255)                                                                    tACGGAAGTTACGGTTGATTTCGAATACCCGTTCAAAACCGCCTACAACCAGACGCTTCAGATACAGCTCCGGCGCGATACGCAGATACATATCTAAATCCAGAGCATTATGATGGGTAATAAACGGGCGAGCAGATGc                                      <  1:280627/139‑1 (MQ=255)                                                                      cGGAAGTTACGGTTGATTTCGAATACCCGTTCAAAACCGCCTACAACCAGACGCTTCAGATACAGCTCCGGCGCGATACGCAGATACATATCTAAATCCAGAGCATTATGATGGGTAATAAACGGGCGAGCAGATGccc                                    <  1:824086/139‑1 (MQ=255)                                                                           gTTACGGTTGATTTCGAATACCCGTTCAAAACCGCCTACAACCAGACGCTTCAGATACAGCTCCGGCGCGATACGCAGATACATATCTAAATCCAGAGCATTATGATGGGTAATAAACGGGCGAGCAGATGCCCCACCt                               >  1:85978/1‑139 (MQ=255)                                                                                     aTTTCGAATACCCGTTCAAAACCGCCTACAACCAGACGCTTCAGATACAGCTCCGGCGCGATACGCAGATACATATCTAAATCCAGAGCATTATGATGGGTAATAAACGGGCGAGCAGATGCCCCAc                                 >  2:98630/1‑127 (MQ=255)                                                                                     aTTTCGAATACCCGTTCAAAACCGCCTACAACCAGACGCTTCAGATACAGCTCCGGCGCGATACGCAGATACATATCTAAATCCAGAGCATTATGATGGGTAATAAACGGGCGAGCAGATGCCCCAc                                 <  1:98630/127‑1 (MQ=255)                                                                                                ccGTTCAAAACCGCCTACAACCAGACGCTTCAGATa                                                                                                                 <  2:506862/36‑1 (MQ=255)                                                                                                ccGTTCAAAACCGCCTACAACCAGACGCTTCAGATa                                                                                                                 >  1:506862/1‑36 (MQ=255)                                                                                                   ttCAAAACCGCCTACAACCAGACGCTTCAGATACAGCTCCGGCGCGATACGCAGATACATATCTAAATCCAGAGCATTATGATGGGTAATAAACGGGCGAGCAGATGCCCCACCTGGAATTACCTGCATCATCGGGGtt       >  2:583622/1‑139 (MQ=255)                                                                                                   ttCAAAACCGCCTACAACCAGACGCTTCAGATACAGCTCCGGCGCGATACGCAGATACATATCTAAATCCAGAGCATTATGATGGGTAATAAACGGGCGAGCAGATGCCCCACCTGGAATTACCTGCATCATCGGGGtt       <  2:85978/139‑1 (MQ=255)                                                                                                     cAAAACCGCCTACAACCAGACGCTTCAGATACAGCTCCGGCGCGATACGCAGATACATATCTAAATCCAGAGCATTATGATGGGTAATAAACGGGCGAGCAGATGCCCCACCTGGAATTACCTGCATCATCGGGGTTTc     >  2:193997/1‑139 (MQ=255)                                                                                                           cGCCTACAACCAGACGCTTCAGATACAGCTCCGGCGCGATACGCAGATACa                                                                                       >  1:949998/1‑51 (MQ=255)                                                                                                           cGCCTACAACCAGACGCTTCAGATACAGCTCCGGCGCGATACGCAGATACa                                                                                       <  2:949998/51‑1 (MQ=255)                                                                                                             ccTACAACCAGACGCTTCAGATACAGCTCCGGCGCGATACGCAGATACATATCTAAATCCAGAGCATTATGATGGGTAATAAACGGGCGAGCAGATGCCCCACCTGGaa                           <  2:1003130/109‑1 (MQ=255)                                                                                                             ccTACAACCAGACGCTTCAGATACAGCTCCGGCGCGATACGCAGATACATATCTAAATCCAGAGCATTATGATGGGTAATAAACGGGCGAGCAGATGCCCCACCTGGaa                           >  1:1003130/1‑109 (MQ=255)                                                                                                                       gACGCTTCAGATACAGCTCCGGCGCGATACGCAGATACATATCTAAATCCAGAGCATTATGATGGGTAATAAACGGGCGAGCAGATGCCCCACCTGGAATTACCTGCATCATCGGGGTTTCTAc  >  1:562782/1‑124 (MQ=255)                                                                                                                       gACGCTTCACATACAGCTCCGGCGCGATACGCAGATACATATCTAAATCCAGAGCATTATGATGGGTAATAAACGGGCGAGCAGATGCCCCACCTGGAATTACCTGCATCATCGGGGTTTCTAc  <  2:562782/124‑1 (MQ=255)                                                                                                                           |                                                                                                                        TCCGCATACGCCATGTAGAGTTCCATCATTGTGAACTCAGGATTATGGCGAACAGAAATACCTTCATTACGGAAGTTACGGTTGATTTCGAATACCCGTTCAAAACCGCCTACAACCAGACGTTTCAGATACAGCTCCGGCGCGATACGCAGATACATATCTAAATCCAGAGCATTATGATGGGTAATAAACGGGCGAGCAGATGCCCCACCTGGAATTACCTGCATCATCGGGGTTTCTAC  >  NC_000913/4353861‑4354102

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑