Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,441,175	A→G	V14A (GTA→GCA)	ytfL ←	UPF0053 family inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,441,175	0	A	G	100.0%	201.3 / NA	55	V14A (GTA→GCA)	ytfL	UPF0053 family inner membrane protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (24/31);  total (24/31)

GTAAAGAACATGCCGGGATTTTCCTGCATATTCAGAACGCGTTGGGCATTTATATTGCCTTCATCAGCCAGCAGTTTAAGTTTGATTTTGCGTGAGGCGGCAAGCGAGATCTCGGACATCGAGAAGAACGCACTTACAGCGATCAAGCAGAGTATGACTAAAATACTGTTTAACATATCTTATCCGACCTTTCAGGCCAGATCCTCGGAAGGGGAAGTTGATTAATTTGTGTGAAATACACATTGAAAGCCGATTCGAAGAAAGTGAA  >  NC_000913/4441040‑4441307                                                                                                                                        |                                                                                                                                     gTAAAGAACATGCCGGGATTTTCCTGCATATTCAGAACGCGTTGGGCATTTATATTGCCTTCATCAGCCAGCAGTTTAAGTTTGATTTTGCGTGAGGCGGCAAGCGAGATCTCGGACATCGAGAAGAACGCACTTGCAg                                                                                                                                   <  1:105746/139‑1 (MQ=255) gTAAAGAACATGCCGGGATTTTCCTGCATAATCAGAAAGCGTTGGGCATTTATATTGCCTTCATCAGCCAGCAGTTTAAGTTTGATTTTGCGTGAGGCGGCAAGCGAGATCTCGGACATCGAGAAGAACGCACTTGCAg                                                                                                                                   <  2:504904/139‑1 (MQ=255)          aTGCCGGGATTTTCCTGCATATTCAGAACGCGTTGGGCATTTATATTGCCTTCATCAGCCAGCAGTTTAAGTTTGATTTTGCGTGAGGCGGCAAGCGAGATCTCGGACATCGAGAAGAACGCACTTGCAGCGATCAAGc                                                                                                                          >  1:856697/1‑139 (MQ=255)           tGCCGGGATTTTCCTGCATATTCAGAACGCGTTGGGCATTTATATTGCCTTCATCAGCCAGCAGTTTAAGTTTGATTTTGCGTGAGGCGGCAAGCGAGATCTCGGACATCGAGAAGAACGCACTTGCAGCGATCAAGCa                                                                                                                         <  1:1006338/139‑1 (MQ=255)                 gATTTTCCTGCATATTCAGAACGCGTTGGGCATTTATATTGCCTTCATCAGCCAGCAGTTTAAGTTTGATTTTGCGTGAGGCGGCAAGCGAGATCTCGGACATCGAGAAGAACGCACTTGCAGCGATCAAGCAGAGTAt                                                                                                                   <  2:202320/139‑1 (MQ=255)                          gCATATTCAGAACGCGTTGGGCATTTATATTGCCTTCATCAGCCAGCAGTTTAAGTTTGATTTTGCGTGAGGCGGCAAGCGAGATCTCGGACATCGAGAAGAACGCACTTGCAGCGATCAAGCAGAGTATGACTAAAAt                                                                                                          <  2:51991/139‑1 (MQ=255)                           cATATTCAGAACGCGTTGGGCATTTATATTGCCTTCATCAGCCAGCAGTTTAAGTTTGATTTTGCGTGAGGCGGCAAGCGAGATCTCGGACATCGAGAAGAACGCACTTGCAGCGATCAAGCAGAGTATGACTAAAATa                                                                                                         <  2:856697/139‑1 (MQ=255)                                tCAGAACGCGTTGGGCATTTATATTGCCTTCATCAGCCAGCAGTTTAAGTTTGATTTTGCGTGAGGCGGCAAGCGAGATCTCGGACATCGAGAAGAACGCACTTGCAg                                                                                                                                   <  2:513967/108‑1 (MQ=255)                                tCAGAACGCGTTGGGCATTTATATTGCCTTCATCAGCCAGCAGTTTAAGTTTGATTTTGCGTGAGGCGGCAAGCGAGATCTCGGACATCGAGAAGAACGCACTTGCAg                                                                                                                                   >  1:513967/1‑108 (MQ=255)                                tCAGAACGCGTTGGGCATTTATATTGCCTTCATCAGCCAGCAGTTTAAGTTTGATTTTGCGTGAGGCGGCAAGCGAGATCTCGGACATCGAGAAGAACGCACTTGCAGCg                                                                                                                                 >  1:208112/1‑110 (MQ=255)                                tCAGAACGCGTTGGGCATTTATATTGCCTTCATCAGCCAGCAGTTTAAGTTTGATTTTGCGTGAGGCGGCAAGCGAGATCTCGGACATCGAGAAGAACGCACTTGCAGCg                                                                                                                                 <  2:208112/110‑1 (MQ=255)                                     aCGCGTTGGGCATTTATATTGCCTTCATCAGCCAGCAGTTTAAGTTTGATTTTGCGTGAGGCGGCAAGCGAGATCTCGGACATCGAGAAGAACGCACTTGCAGCGATCAAGCAGAGt                                                                                                                     >  2:819632/1‑117 (MQ=255)                                     aCGCGTTGGGCATTTATATTGCCTTCATCAGCCAGCAGTTTAAGTTTGATTTTGCGTGAGGCGGCAAGCGAGATCTCGGACATCGAGAAGAACGCACTTGCAGCGATCAAGCAGAGt                                                                                                                     <  1:819632/117‑1 (MQ=255)                                          ttGGGCATTTATATTGCCTTCATCAGCCAGCAGTTTAAGTTTGATTTTGCGTGAGGCGGCAAGCGAGATCTCGGACATCGAGAAGAACGCACTTGCAg                                                                                                                                   <  2:760021/98‑1 (MQ=255)                                          ttGGGCATTTATATTGCCTTCATCAGCCAGCAGTTTAAGTTTGATTTTGCGTGAGGCGGCAAGCGAGATCTCGGACATCGAGAAGAACGCACTTGCAg                                                                                                                                   >  1:760021/1‑98 (MQ=255)                                          ttGGGCATTTATATTGCCTTCATCAGCCAGCAGTTTAAGTTTGATTTTGCGTGAGGCGGCAAGCGAGATCTCGGACATCGAGAAGAACGCACTTGCAGCGATCAAGCAGAGTATGACTAAAATACTGTTTAACATATCt                                                                                          >  2:175320/1‑139 (MQ=255)                                                aTTTATATTGCCTTCATCAGCCAGCAGTTTAAGTTTGATTTTGCGTGAGGCGGCAAGCGAGATCTCGGACATCGAGAAGAACGCACTTGCAGCGATCAAGCAGAGTATGACTAAAATACTGTTTAACATATCTTATCCg                                                                                    <  2:781541/139‑1 (MQ=255)                                                  ttATATTGCCTTCATCAGCCAGCAGTTTAAGTTTGATTTTGCGTGAGGCGGCAAGCGAGATCTCGGACATCGAGAAGAACGCACTTGCAGCGATCAAGCAGAGTATGACTAAAATACTGTTTAACATATCTTATCCGa                                                                                   <  1:523600/138‑1 (MQ=255)                                                  ttATATTGCCTTCATCAGCCAGCAGTTTAAGTTTGATTTTGCGTGAGGCGGCAAGCGAGATCTCGGACATCGAGAAGAACGCACTTGCAGCGATCAAGCAGAGTATGACTAAAATACTGTTTAACATATCTTATCCGa                                                                                   >  2:523600/1‑138 (MQ=255)                                                      atTGCCTTCATCAGCCAGCAGTTTAAGTTTGATTTTGCGTGAGGCGGCAAGCGAGATCTCGGACATCGAGAAGAACGCACTTGCAGCGATCAAGCAGAGTATGACTAAAATACTGTTTAACATATCTTATCCGACCttt                                                                              <  2:850315/139‑1 (MQ=255)                                                            ttCATCAGCCAGCAGTTTAAGTTTGATTTTGCGTGAGGCGGCAAGCGAGATCTCGGACATCGAGAAGAACGCACTTGCAGCGATc                                                                                                                              <  1:847592/85‑1 (MQ=255)                                                            ttCATCAGCCAGCAGTTTAAGTTTGATTTTGCGTGAGGCGGCAAGCGAGATCTCGGACATCGAGAAGAACGCACTTGCAGCGATc                                                                                                                              >  2:847592/1‑85 (MQ=255)                                                            ttCATCAGCCAGCAGTTTAAGTTTGATTTTGCGTGAGGCGGCAAGCGAGATCTCGGACATCGAGAAGAACGCACTTGCAGCGATCAAGCAGAGTATGACTAAAATACTg                                                                                                      >  2:91624/1‑109 (MQ=255)                                                            ttCATCAGCCAGCAGTTTAAGTTTGATTTTGCGTGAGGCGGCAAGCGAGATCTCGGACATCGAGAAGAACGCACTTGCAGCGATCAAGCAGAGTATGACTAAAATACTg                                                                                                      <  1:91624/109‑1 (MQ=255)                                                            ttCATCAGCCAGCAGTTTAAGTTTGATTTTGCGTGAGGCGGCAAGCGAGATCTCGGACATCGAGAAGAACGCACTTGCAGCGATCAAGCAGAGTATGACTAAAATACTGTTTAACATATCTTATCCGACCTTTCAGGcc                                                                        >  2:517185/1‑139 (MQ=255)                                                              catcaGCCAGCAGTTTAAGTTTGATTTTGCGTGAGGCGGCAAGCGAGATCTCGGACATCGAGAAGAACGCACTTGCAGCGATCAAGCAGAGt                                                                                                                     <  2:223592/92‑1 (MQ=255)                                                              catcaGCCAGCAGTTTAAGTTTGATTTTGCGTGAGGCGGCAAGCGAGATCTCGGACATCGAGAAGAACGCACTTGCAGCGATCAAGCAGAGt                                                                                                                     >  1:223592/1‑92 (MQ=255)                                                              catcaGCCAGCAGTTTAAGTTTGATTTTGCGTGAGGCGGCAAGCGAGATCTCGGACATCGAGAAGAACGCACTTGCAGCGATCAAGCAGAGTAt                                                                                                                   >  1:442943/1‑94 (MQ=255)                                                              catcaGCCAGCAGTTTAAGTTTGATTTTGCGTGAGGCGGCAAGCGAGATCTCGGACATCGAGAAGAACGCACTTGCAGCGATCAAGCAGAGTAt                                                                                                                   <  2:442943/94‑1 (MQ=255)                                                               atcaGCCAGCAGTTTAAGTTTGATTTTGCGTGAGGCGGCAAGCGAGATCTCGGACATCGAGAAGAACGCACTTGCAGCGa                                                                                                                                <  2:838067/80‑1 (MQ=255)                                                               atcaGCCAGCAGTTTAAGTTTGATTTTGCGTGAGGCGGCAAGCGAGATCTCGGACATCGAGAAGAACGCACTTGCAGCGa                                                                                                                                >  1:838067/1‑80 (MQ=255)                                                               atcaGCCAGCAGTTTAAGTTTGATTTTGCGTGAGGCGGCAAGCGAGATCTCGGACATCGAGAAGAACGCACTTGCAGCGATCAAGCAGAGTATGACTAAAATACTGTTTAACATATCTTATCCGACCTTTCAGGCCAGa                                                                     >  2:42582/1‑139 (MQ=255)                                                                             tAAGTTTGATTTTGCGTGAGGCGGCAAGCGAGATCTCGGACATCGAGAAGAACGCACTTGCAGCGATCAAg                                                                                                                           >  1:462134/1‑71 (MQ=255)                                                                             tAAGTTTGATTTTGCGTGAGGCGGCAAGCGAGATCTCGGACATCGAGAAGAACGCACTTGCAGCGATCAAg                                                                                                                           <  2:462134/71‑1 (MQ=255)                                                                                  ttGATTTTGCGTGAGGCGGCAAGCGAGATCTCGGACATCGAGAAGAACGCACTTGCAGCGATCAAg                                                                                                                           <  2:788304/66‑1 (MQ=255)                                                                                  ttGATTTTGCGTGAGGCGGCAAGCGAGATCTCGGACATCGAGAAGAACGCACTTGCAGCGATCAAg                                                                                                                           >  1:788304/1‑66 (MQ=255)                                                                                   tGATTTTGCGTGAGGCGGCAAGCGAGATCTCGGACATCGAGAAGAACGCACTTGCAGCGATCAAg                                                                                                                           >  2:718647/1‑65 (MQ=255)                                                                                   tGATTTTGCGTGAGGCGGCAAGCGAGATCTCGGACATCGAGAAGAACGCACTTGCAGCGATCAAg                                                                                                                           <  1:718647/65‑1 (MQ=255)                                                                                     aTTTTGCGTGAGGCGGCAAGCGAGATCTCGGACATCGAGAAGAACGCACTTGCAGCGATCAAGCa                                                                                                                         >  1:669913/1‑65 (MQ=255)                                                                                     aTTTTGCGTGAGGCGGCAAGCGAGATCTCGGACATCGAGAAGAACGCACTTGCAGCGATCAAGCa                                                                                                                         <  2:669913/65‑1 (MQ=255)                                                                                        ttGCGTGCGGCGGCAAGCGATATCTCGGACATCGAGAAGAACGCACTTGCAGCGATCAAGCAGAGTATGACTAAAATACTGTTTCACATATCTTATCCGACCTTTCAGGCCAGATCCTCGGAAGGGGAAGTTGATTAAt                                            <  1:207089/139‑1 (MQ=255)                                                                                            gTGAGGCGGCAAGCGAGATCTCGGACATCGAGAAGAACGCACTTGCAGCGATCAAGCAGAGTATGACTAAAATACTGTTTAACATATCTTATCCGACCTTTCAGGCCAGATCCTCGGAAGGGGAAGTTGATTAATTtgt                                        <  1:517185/139‑1 (MQ=255)                                                                                               aGGCGGCAAGCGAGATCTCGGACATCGAGAAGAACGCACTTGCAGCGATCAAGCAGAGTATGACTAAAATACTGTTTAACATATCTTATCCGACCTTTCAGGCCAGATCCTCGGAAGGGGAAGTTGATTAATTtgtgtg                                     >  1:197196/1‑139 (MQ=255)                                                                                               aGGCGGCAAGCGAGATCTCGGACATCGAGAAGAACGCACTTGCAGCGATCAAGCAGAGTATGACTAAAATACTGTTTAACATATCTTATCCGACCTTTCAGGCCAGATCCTCGGAAGGGGAAGTTGATTAATTtgt                                        <  2:1003361/136‑1 (MQ=255)                                                                                               aGGCGGCAAGCGAGATCTCGGACATCGAGAAGAACGCACTTGCAGCGATCAAGCAGAGTATGACTAAAATACTGTTTAACATATCTTATCCGACCTTTCAGGCCAGATCCTCGGAAGGGGAAGTTGATTAATTtgt                                        >  1:1003361/1‑136 (MQ=255)                                                                                                  cggcAAGCGAGATCTCGGACATCGAGAAGAACGCACTTGCAGCGATCAAGCAGAGTATGACTAAAATACTGTTTAACATATCTTATCCGACCTTTCAGGCCAGATCCTCGGAAGGGGAAGTTGATTAATTTGTGTGaaa                                  <  2:481481/139‑1 (MQ=255)                                                                                                                  ggACATCGAGAAGAACGCACTTGCAGCGATCAAGCAGAGTATGACTAAAATACTGTTTAACATATCTTATCCGACCTTTCAGGCCAGATCCTCg                                                               >  2:94438/1‑94 (MQ=255)                                                                                                                  ggACATCGAGAAGAACGCACTTGCAGCGATCAAGCAGAGTATGACTAAAATACTGTTTAACATATCTTATCCGACCTTTCAGGCCAGATCCTCg                                                               <  1:94438/94‑1 (MQ=255)                                                                                                                                 cGCACTTGCAGCGATCAAGCAGAGTATGACTAAAATACTGTTTAACATATCTTATCCGACCTTTCAGGCCAGATCCTCGGAAGGGGAAGTTGATTAATTTGTGTGa                                    <  1:327483/106‑1 (MQ=255)                                                                                                                                 cGCACTTGCAGCGATCAAGCAGAGTATGACTAAAATACTGTTTAACATATCTTATCCGACCTTTCAGGCCAGATCCTCGGAAGGGGAAGTTGATTAATTTGTGTGa                                    <  1:300793/106‑1 (MQ=255)                                                                                                                                 cGCACTTGCAGCGATCAAGCAGAGTATGACTAAAATACTGTTTAACATATCTTATCCGACCTTTCAGGCCAGATCCTCGGAAGGGGAAGTTGATTAATTTGTGTGa                                    >  2:300793/1‑106 (MQ=255)                                                                                                                                 cGCACTTGCAGCGATCAAGCAGAGTATGACTAAAATACTGTTTAACATATCTTATCCGACCTTTCAGGCCAGATCCTCGGAAGGGGAAGTTGATTAATTTGTGTGa                                    >  2:327483/1‑106 (MQ=255)                                                                                                                                  gCACTTGCAGCGATCAAGCAGAGTATGACTAAAATACTGTTTAACATATCTTATCCGACCTTTCAGGCCAGATCCTCGGAAGGGGAAGTTGATTAATTTGTGTGAAATACACATTGAAAGCCGATTCGAAGAAAGTGaa  <  1:175320/139‑1 (MQ=255)                                                                                                                                    aCTTGCAGCGATCAAGCAGAGTATGACTAAAATACTGTTTAACATATCTTATCCGACCTTTCAGGCCAGATCCTCg                                                               >  2:288843/1‑76 (MQ=255)                                                                                                                                    aCTTGCAGCGATCAAGCAGAGTATGACTAAAATACTGTTTAACATATCTTATCCGACCTTTCAGGCCAGATCCTCg                                                               <  1:288843/76‑1 (MQ=255)                                                                                                                                        |                                                                                                                                     GTAAAGAACATGCCGGGATTTTCCTGCATATTCAGAACGCGTTGGGCATTTATATTGCCTTCATCAGCCAGCAGTTTAAGTTTGATTTTGCGTGAGGCGGCAAGCGAGATCTCGGACATCGAGAAGAACGCACTTACAGCGATCAAGCAGAGTATGACTAAAATACTGTTTAACATATCTTATCCGACCTTTCAGGCCAGATCCTCGGAAGGGGAAGTTGATTAATTTGTGTGAAATACACATTGAAAGCCGATTCGAAGAAAGTGAA  >  NC_000913/4441040‑4441307

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑