Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,565,966 C→T *427* (TAG→TAA)  yjiN ← zinc‑type alcohol dehydrogenase‑like protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,565,9660CT100.0% 149.2 / NA 46*427* (TAG→TAA) yjiNzinc‑type alcohol dehydrogenase‑like protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (25/21);  total (25/21)

ATCGGTGACAAGTGACATGATTTTCCTTCCCCGTACCACGGGAGATAATGAAAAGAGGCCACATTATTACCATTCTTTTAGTATGGTTTATTTGATCTCTCTCGCGGTTAGCCACTTAGTTTTTCATGGATTTCTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAATATAAAATTAGCCCAATACAACCGCCAACCAGCGTACCGTTGACACGAATAAACTGCAGATCTTTGCCGATATT  >  NC_000913/4565833‑4566085
                                                                                                                                     |                                                                                                                       
aTCGGTGACAAGTGACATGATTTTCCTTCCCCGTACCACGGGAGATAATGAAAAGAGGCCACATTATTACCATTCTTTTAGTATGGTTTATTTGATCTCTCTCGCGGTTAGCCACTTAGTTTTTCATGGATTTTTaaaa                                                                                                                    >  2:678761/1‑139 (MQ=255)
                     tttCCTTCCCCGTACCACGGGAGATAATGAAAAGAGGCCACATTATTACCATTCTTTTAGTATGGTTTATTTGATCTCTCTCGCGGTTAGCCACTTAGTTTTTCATGGATTTTTAAAAATTGCCGAGGGGGAACAAGGc                                                                                               >  2:928905/1‑139 (MQ=255)
                                  aCCACGGGAGATAATGAAAAGAGGCCACATTATTACCATTCTTTTAGTATGGTTTATTTGATCTCTCTCGCGGTTAGCCACTTAGTTTTTCATGGATTTTTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGAc                                                                                  >  2:124325/1‑139 (MQ=255)
                                     aCGGGAGATAATGAAAAGAGGCCACATTATTACCATTCTTTTAGTATGGTTTATTTGATCTCTCTCGCGGTTAGCCACTTAGTTTTTCATGGATTTTTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGc                                                                               <  1:155348/139‑1 (MQ=255)
                                      cGGGAGATAATGAAAAGAGGCCACATTATTACCATTCTTTTAGTATGGTTTATTTGATCTCTCTCGCGGTTAGCCACTTAGTTTTTCATGGATTTTTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCa                                                                              >  2:65874/1‑139 (MQ=255)
                                            aTAATGAAAAGAGGCCACATTATTACCATTCTTTTAGTATGGTTTATTTGATCTCTCTCGCGGTTAGCCACTTAGTTTTTCATGGATTTTTAAAAATTGc                                                                                                               <  1:250100/100‑1 (MQ=255)
                                            aTAATGAAAAGAGGCCACATTATTACCATTCTTTTAGTATGGTTTATTTGATCTCTCTCGCGGTTAGCCACTTAGTTTTTCATGGATTTTTAAAAATTGc                                                                                                               >  2:250100/1‑100 (MQ=255)
                                                tGAAAAGAGGCCACATTATTACCATTCTTTTAGTATGGTTTATTTGATCTCTCTCGCGGTTAGCCACTTAGTTTTTCATGGATTTTTAAAAATTGCCGAGGGGGAACAAGGCCggg                                                                                           >  2:68070/1‑116 (MQ=255)
                                                tGAAAAGAGGCCACATTATTACCATTCTTTTAGTATGGTTTATTTGATCTCTCTCGCGGTTAGCCACTTAGTTTTTCATGGATTTTTAAAAATTGCCGAGGGGGAACAAGGCCggg                                                                                           <  1:68070/116‑1 (MQ=255)
                                                      gagGCCACATTATTACCATTCTTTTAGTATGGTTTATTTGATCTCTCTCGCGGTTAGCCACTTAGTTTTTCATGGATTTTTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAATATAAAATTAGccc                                                              <  2:122072/139‑1 (MQ=255)
                                                                     ccATTCTTTTAGTATGGTTTATTTGATCTCTCTCGCGGTTAGCCACTTAGTTTTTCATGGATTTTTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAATATAAAATTAGCCCAATACAACCGCCAAc                                               <  1:65874/139‑1 (MQ=255)
                                                                          cTTTTAGTATGGTTTATTTGATCTCTCACGCGGTTAGCCACTTAGTTTTTCATGGATTTTTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAATATAAAATTAGCCCAATACAATCGCCAACCAGCg                                          >  1:602726/1‑139 (MQ=255)
                                                                             ttAGTATGGTTTATTTGATCTCTCTCGCGGTTAGCCACTTAGTTTTTCATGGATTTTTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCa                                                                              >  2:508536/1‑100 (MQ=255)
                                                                             ttAGTATGGTTTATTTGATCTCTCTCGCGGTTAGCCACTTAGTTTTTCATGGATTTTTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCa                                                                              <  1:508536/100‑1 (MQ=255)
                                                                                gTATGGTTTATTTGATCTCTCTCGCGGTTAGCCACTTAGTTTTTCATGGATTTTTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAATATAAAATTAGCCCAATACAACCGCCAACCAGCGTACCGt                                    >  2:571963/1‑139 (MQ=255)
                                                                                     gTTTATTTGATCTCTCTCGCGGTTAGCCACTTAGTTTTTCATGGATTTTTAAAAATTGCCGAgg                                                                                                          >  1:520150/1‑64 (MQ=255)
                                                                                     gTTTATTTGATCTCTCTCGCGGTTAGCCACTTAGTTTTTCATGGATTTTTAAAAATTGCCGAgg                                                                                                          <  2:520150/64‑1 (MQ=255)
                                                                                     gTTTATTTGATCTCTCTCGCGGTTAGCCACTTAGTTTTTCATGGATTTTTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAATATAAAATTAGCCCAATACAACCGCCAACCAGCGTACCGTTGaca                               >  2:341443/1‑139 (MQ=255)
                                                                                          tttGATCTCTCTCGCGGTTAGCCACTTAGTTTTTCATGGATTTTTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAATATAAAATTAGCCCAATACAACCGCCaa                                                >  1:705603/1‑117 (MQ=255)
                                                                                          tttGATCTCTCTCGCGGTTAGCCACTTAGTTTTTCATGGATTTTTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAATATAAAATTAGCCCAATACAACCGCCaa                                                <  2:705603/117‑1 (MQ=255)
                                                                                          tttGATCTCTCTCGCGGTTAGCCACTTAGTTTTTCATGGATTTTTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAATATAAAATTAGCCCAATACAACCGCCAACCAGCGTACCGTTGACACGAAt                          <  2:840458/139‑1 (MQ=255)
                                                                                           ttGATCTCTCTCGCGGTTAGCCACTTAGTTTTTCATGGATTTTTAAAAATTGc                                                                                                               >  1:29156/1‑53 (MQ=255)
                                                                                           ttGATCTCTCTCGCGGTTAGCCACTTAGTTTTTCATGGATTTTTAAAAATTGc                                                                                                               <  2:29156/53‑1 (MQ=255)
                                                                                           ttGATCTCTCTCGCGGTTAGCCACTTAGTTTTTCATGGATTTTTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAATATAAAATTAGCCCAATACAACCGCCAACCAGCGTACCGTTGACACGAATa                         <  2:206953/139‑1 (MQ=255)
                                                                                              aTCTCTCTCGCGGTTAGCCACTTAGTTTTTCATGGATTTTTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAATATAAAATTAGCCCAATACAACCGCCAACCAGCGTACCGTTGACACGAATa                         >  1:58847/1‑136 (MQ=255)
                                                                                              aTCTCTCTCGCGGTTAGCCACTTAGTTTTTCATGGATTTTTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAATATAAAATTAGCCCAATACAACCGCCAACCAGCGTACCGTTGACACGAATa                         <  2:58847/136‑1 (MQ=255)
                                                                                               tctctctcGCGGTTAGCCACTTAGTTTTTCATGGATTTTTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGc                                                                               <  2:41637/81‑1 (MQ=255)
                                                                                               tctctctcGCGGTTAGCCACTTAGTTTTTCATGGATTTTTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGc                                                                               >  1:41637/1‑81 (MQ=255)
                                                                                               tctctctcGCGGTTAGCCACTTAGTTTTTCATGGATTTTTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAATATAAAATTAGCCCAATACAAc                                                      <  2:469803/106‑1 (MQ=255)
                                                                                               tctctctcGCGGTTAGCCACTTAGTTTTTCATGGATTTTTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAATATAAAATTAGCCCAATACAAc                                                      >  1:469803/1‑106 (MQ=255)
                                                                                               tctctctcGCGGTTAGCCACTTAGTTTTTCATGGATTTTTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAATATAAAATTAGCCCAATACAACCGCCAACCAGCGTACCGTTGACACGAATAAACt                     >  2:863664/1‑139 (MQ=255)
                                                                                                ctctctcGCGGTTAGCCACTTAGTTTTTCATGGATTTTTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCa                                                                              >  2:559713/1‑81 (MQ=255)
                                                                                                ctctctcGCGGTTAGCCACTTAGTTTTTCATGGATTTTTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCa                                                                              <  1:559713/81‑1 (MQ=255)
                                                                                                    ctcGCGGTTAGCCACTTAGTTTTTCATGGATTTTTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAATATAAAATTAGCCCAATACAACCGCCAACCAGCGTACCGTTGACACGAATAAACTGCAGa                >  1:313346/1‑139 (MQ=255)
                                                                                                    ctcGCGGTTAGCCACTTAGTTTTTCATGGATTTTTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGAAAATATAAAATTAGCCCAATACAACCGCCAACCAGCGTACCGTTGACACGAATAAACTGCAGa                >  2:608175/1‑139 (MQ=255)
                                                                                                             aGCCACTTAGTTTTTCATGGATTTTTAAAAATTGCCGAGGGGGAACAAGGCCg                                                                                             <  1:124476/53‑1 (MQ=255)
                                                                                                             aGCCACTTAGTTTTTCATGGATTTTTAAAAATTGCCGAGGGGGAACAAGGCCg                                                                                             >  2:124476/1‑53 (MQ=255)
                                                                                                                  cTTAGTTTTTCATGGATTTTTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAATATAAAATTAGCCCAATACAACCGCCAACCAGCGTACCGTTGACACGAATAAACTGCAGATCTTTGCCGATAtt  <  1:928905/139‑1 (MQ=255)
                                                                                                                     aGTTTTTCATGGATTTTTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAATATAAAATTAGCCCAATACAAcc                                                     <  2:314653/85‑1 (MQ=255)
                                                                                                                     aGTTTTTCATGGATTTTTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAATATAAAATTAGCCCAATACAAcc                                                     >  1:314653/1‑85 (MQ=255)
                                                                                                                          tacATGGATTTTTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAg                                                                                <  2:1213/51‑1 (MQ=255)
                                                                                                                          tacATGGATTTTTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAg                                                                                >  1:1213/3‑53 (MQ=255)
                                                                                                                                 aTTTTTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAatat                                                                         >  2:140821/1‑53 (MQ=255)
                                                                                                                                 aTTTTTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAatat                                                                         <  1:140821/53‑1 (MQ=255)
                                                                                                                                 aTTTTTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAATATAAAATTAGCCCAATACAACCGCCAACCAGCGTACCGtt                                   >  1:105469/1‑91 (MQ=255)
                                                                                                                                 aTTTTTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAATATAAAATTAGCCCAATACAACCGCCAACCAGCGTACCGtt                                   <  2:105469/91‑1 (MQ=255)
                                                                                                                                     |                                                                                                                       
ATCGGTGACAAGTGACATGATTTTCCTTCCCCGTACCACGGGAGATAATGAAAAGAGGCCACATTATTACCATTCTTTTAGTATGGTTTATTTGATCTCTCTCGCGGTTAGCCACTTAGTTTTTCATGGATTTCTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAATATAAAATTAGCCCAATACAACCGCCAACCAGCGTACCGTTGACACGAATAAACTGCAGATCTTTGCCGATATT  >  NC_000913/4565833‑4566085

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑