Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,577,958 C→T M1M (GTG→ATG) †
*460* (TAG→TAA
mcrC ←
mcrB ←
5‑methylcytosine‑specific restriction enzyme McrBC, subunit McrC
5‑methylcytosine‑specific restriction enzyme McrBC, subunit McrB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,577,9580CT100.0% 180.2 / NA 56M1M (GTG→ATG) †
*460* (TAG→TAA
mcrC
mcrB
5‑methylcytosine‑specific restriction enzyme McrBC, subunit McrC
5‑methylcytosine‑specific restriction enzyme McrBC, subunit McrB
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (33/23);  total (33/23)

ACCCCAGGATATCAAGAAGATTGTTACCGGGTATGGCTTCAAGGTTTGCCTGCTTAATTTCCTGTAAATAACCCCATGCATAGGTAAGCATGTAATAGATATTACGGACAGGTATCACGGGCTGTTCCACTATGAGTCCCCTAATAATTTGTTGGTCCATTTCTGTTGTTTATAGGGGTCATCAAA‑‑‑‑‑GAAATATTCTTCGAGTAAAGGGGCGATATCCGTCATCACAATTTCATTAAGCCATTGCGTATCCGGAGAGGTGCCAT  >  NC_000913/4577829‑4578091
                                                                                                                                 |                                                                                                                                          
acCCCAGGATATCAAGAAGATTGTTACCGGGTATGGCTTCAAGGTTTGCCTGCTTAATTTCCTGTAAATAACCCCATGCATAGGTAAGCATGTAATAGATATTACGGACAGGTATCACGGGCTGTTCCATTATGAg                                                                                                                                      <  1:462903/136‑1 (MQ=255)
acCCCAGGATATCAAGAAGATTGTTACCGGGTATGGCTTCAAGGTTTGCCTGCTTAATTTCCTGTAAATAACCCCATGCATAGGTAAGCATGTAATAGATATTACGGACAGGTATCACGGGCTGTTCCATTATGAg                                                                                                                                      >  2:462903/1‑136 (MQ=255)
acCCCAGGATATCAAGAAGATTGTTACCGGGTATGGCTTCAAGGTTTGCCTGCTTAATTTCCTGTAAATAACCCCATGCATAGGTAAGCATGTAATAGATATTACGGACAGGTATCACGGGCTGTTCCATTATGAGTcc                                                                                                                                   >  2:160010/1‑139 (MQ=255)
acCCCAGGATATCAAGAAGATTGTTACCGGGTATGGCTTCAAGGTTTGCCTGCTTAATTTCCTGTAAATAACCCCATGCATAGGTAAGCATGTAATAGATATTACGGACAGGTATCACGGGCTGTTCCATTATGAGTcc                                                                                                                                   >  2:41624/1‑139 (MQ=255)
acCCCAGGATATCAAGAAGATTGTTACCGGGTATGGCTTCAAGGTTTGCCTGCTTAATTTCCTGTAAATAACCACATGCATAGGTAAGCATGTAATAGATATTACGGACAGGTATAACGGGCTGTTCCATTATGAGtac                                                                                                                                   >  1:682456/1‑137 (MQ=255)
          atCAAGAAGATTGTTACCGGGTATGGCTTCAAGGTTTGCCTGCTTAATTTCCTGTAAATAACCCCATGCATAGGTAAGCATGTAATAGATATTACGGACAGGTATCACGGGCTGTTCCATTATg                                                                                                                                        >  1:702772/1‑124 (MQ=255)
          atCAAGAAGATTGTTACCGGGTATGGCTTCAAGGTTTGCCTGCTTAATTTCCTGTAAATAACCCCATGCATAGGTAAGCATGTAATAGATATTACGGACAGGTATCACGGGCTGTTCCATTATg                                                                                                                                        <  2:702772/124‑1 (MQ=255)
          atCAAGAAGATTGTTACCGGGTATGGCTTCAAGGTTTGCCTGCTTAATTTCCTGTAAATAACCCCATGCATAGGTAAGCATGTAATAGATATTACGGACAGGTATCACGGGCTGTTCCATTATGAGTCCCCTAATAAtt                                                                                                                         >  2:173233/1‑139 (MQ=255)
            cAAGAAGATTGTTACCGGGTATGGCTTCAAGGTTTGCCTGCTTAATTTCCTGTAAATAACCCCATGCATAGGTAAGCATGTAATAGATATTACGGACAGGTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATttg                                                                                                                       >  1:491285/1‑139 (MQ=255)
            cAAGAAGATTGTTACCGGGTATGGCTTCAAGGTTTGCCTGCTTAATTTCCTGTAAATAACCCCATGCATAGGTAAGCATGTAATAGATATTACGGACAGGTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATttg                                                                                                                       >  1:686956/1‑139 (MQ=255)
             aagaagATTGTTACCGGGTATGGCTTCAAGGTTTGCCTGCTTAATTTCCTGTAAATAACCCCATGCATAGGTAAGCATGTAATAGATATTACGGACAGGTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATttgt                                                                                                                      <  1:160010/139‑1 (MQ=255)
             aagaagATTGTTACCGCGTATGGCTTCAAGGTTTGCCTGCTTAATTTCCTGTAAATAACCCCATGCATAGGTAAGCATGTAATAGATATTACGGACAGGTATCACGGGCTGTTCCATTGTGAGTCCCCTAATAATttgt                                                                                                                      <  2:686956/139‑1 (MQ=255)
                  gaTTGTTACCGGGTATGGCTTCAAGGTTTGCCTGCTTAATTTCCTGTAAATAACCCCATGCATAGGTAAGCATGTAATAGATATTACGGACAGGTATCACGGGCTGTTCCATTATg                                                                                                                                        <  2:894045/116‑1 (MQ=255)
                  gaTTGTTACCGGGTATGGCTTCAAGGTTTGCCTGCTTAATTTCCTGTAAATAACCCCATGCATAGGTAAGCATGTAATAGATATTACGGACAGGTATCACGGGCTGTTCCATTATg                                                                                                                                        >  1:894045/1‑116 (MQ=255)
                     tGTTACCGGGTATGGCTTCAAGGTTTGCCTGCTTAATTTCCTGTAAATAACCCCATGCATAGGTAAGCATGTAATAGATATTACGGACAGGTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCAt                                                                                                              >  2:314544/1‑139 (MQ=255)
                     tGTTACCGGGTATGGCTTCAAGGTTTGCCTGCTTAATTTCCTGTAAATAACCCCATGCATAGGTAAGCATGTAATAGATATTACGGACAGGTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCAt                                                                                                              <  2:491285/139‑1 (MQ=255)
                       ttACCGGGTATGGCTTCAAGGTTTGCCTGCTTAATTTCCTGTAAATAACCCCATGCATAGGTAAGCATGTAATAGATATTACGGACAGGTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCAttt                                                                                                            >  2:752524/1‑139 (MQ=255)
                         aCCGGGTATGGCTTCAAGGTTTGCCTGCTTAATTTCCTGTAAATAACCCCATGCATAGGTAAGCATGTAATAGATATTACGGACAGGTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCt                                                                                                          <  1:41624/139‑1 (MQ=255)
                          ccGGGTATGGCTTCAAGGTTTGCCTGCTTAATTTCCTGTAAATAACCCCATGCATAGGTAAGCATGTAATAGATATTACGGACAGGTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCtg                                                                                                         <  1:314544/139‑1 (MQ=255)
                             ggTATGGCTTCAAGGTTTGCCTGCTTAATTTCCTGTAAATAACCCCATGCATAGGTAAGCATGTAATAGATATTACGGACAGGTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCtgttg                                                                                                      >  2:11427/1‑139 (MQ=255)
                              gTATGGCTTCAAGGTTTGCCTGCTTAATTTCCTGTAAATAACCCCATGCATAGGTAAGCATGTAATAGATATTACGGACAGGTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCtgttgt                                                                                                     >  2:936494/1‑139 (MQ=255)
                                 tGGCTTCAAGGTTTGCCTGCTTAATTTCCTGTAAATAACCCCATGCATAGGTAAGCATGTAATAGATATTACGGACAGGTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCtgtt                                                                                                       >  2:85755/1‑134 (MQ=255)
                                 tGGCTTCAAGGTTTGCCTGCTTAATTTCCTGTAAATAACCCCATGCATAGGTAAGCATGTAATAGATATTACGGACAGGTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCtgtt                                                                                                       <  1:85755/134‑1 (MQ=255)
                                     ttCAAGGTTTGCCTGCTTAATTTCCTGTAAATAACCCCATGCATAGGTAAGCATGTAATAGATATTACGGACAGGTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCTGTTGTTTATAgg                                                                                              <  1:752524/139‑1 (MQ=255)
                                                   gCTTAATTTCCTGTAAATAACCCCATGCATAGGTAAGCATGTAATAGATATTACGGACAGGTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCTGTTGTTTATAGGGGTCATCAAA‑‑‑‑‑Gaaa                                                                           <  1:11427/139‑1 (MQ=255)
                                                    cTTAATTTCCTGTAAATAACCCCATGCATAGGTAAGCATGTAATAGATATTACGGACAGGTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCTGTTGTTTATAGGGGTCATCAAA‑‑‑‑‑GAAat                                                                          >  2:184055/1‑139 (MQ=255)
                                                         tttCCTGTAAATAACCCCATGCATAGGTAAGCATGTAATAGATATTACGGACAGGTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCTGTTGTTTATAGGGGTCATCAAA‑‑‑‑‑GAAATAttct                                                                     <  1:184055/139‑1 (MQ=255)
                                                               gTAAATAACCCCATGCATAGGTAAGCATGTAATAGATATTACGGACAGGTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCtgtt                                                                                                       >  2:527696/1‑104 (MQ=255)
                                                               gTAAATAACCCCATGCATAGGTAAGCATGTAATAGATATTACGGACAGGTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCtgtt                                                                                                       <  1:527696/104‑1 (MQ=255)
                                                                        cccATGCATAGGTAAGCAAGTAATAGATATTACGGACAGGTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCTGTTGTTTATAGGGGTCATCAAA‑‑‑‑‑GAAATATTCTTCGAGTAAAGTGGCg                                                      >  2:665446/1‑139 (MQ=255)
                                                                            tGCATAGGTAAGCATGTAATAGATATTACGGACAGGTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCTGTTGTTTATAGGGGTCATCAAA‑‑‑‑‑GAAATATTCTTCGAGTAAAGGGGCGatat                                                  >  1:554397/1‑139 (MQ=255)
                                                                                      aGCATGTAATAGATATTACGGACAGGTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCt                                                                                                          >  2:282828/1‑78 (MQ=255)
                                                                                      aGCATGTAATAGATATTACGGACAGGTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCt                                                                                                          <  1:282828/78‑1 (MQ=255)
                                                                                      aGCATGTAATAGATATTACGGACAGGTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCTGTTGTTTATAGGGGTCATCAAAGACAGGAAATATTCTTCGAGTAAAGGGGCGATATCCGtc                                             >  1:488436/1‑139 (MQ=255)
                                                                                      aGCATGTAATAGATATTACGGACAGGTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCTGTTGTTTATAGGGGTCATCAAA‑‑‑‑‑GAAATATTCTTCGAGTAAAGGGGCGATATCCGTCATcac                                        >  2:360342/1‑139 (MQ=255)
                                                                                            tAATAGATATTACGGACAGGTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCtg                                                                                                         <  1:80616/73‑1 (MQ=255)
                                                                                            tAATAGATATTACGGACAGGTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCtg                                                                                                         >  2:80616/1‑73 (MQ=255)
                                                                                                  atatTACGGACAGGTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCAttt                                                                                                            >  2:22915/1‑64 (MQ=255)
                                                                                                  atatTACGGACAGGTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCAttt                                                                                                            <  1:22915/64‑1 (MQ=255)
                                                                                                    atTACGGACAGGTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATttgt                                                                                                                      >  1:317304/1‑52 (MQ=255)
                                                                                                    atTACGGACAGGTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATttgt                                                                                                                      <  2:317304/52‑1 (MQ=255)
                                                                                                      tACGGACAGGTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCtgt                                                                                                        >  1:326362/1‑64 (MQ=255)
                                                                                                      tACGGACAGGTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCtgt                                                                                                        <  2:326362/64‑1 (MQ=255)
                                                                                                      tACGGACAGGTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCTGTTGTTTATAGGGGTCATCAAA‑‑‑‑‑GAAATATTCTTCGAGTAAAGGGGCGATATCCGTCATCACAAt                                     <  2:30568/126‑1 (MQ=255)
                                                                                                      tACGGACAGGTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCTGTTGTTTATAGGGGTCATCAAA‑‑‑‑‑GAAATATTCTTCGAGTAAAGGGGCGATATCCGTCATCACAAt                                     >  1:30568/1‑126 (MQ=255)
                                                                                                      tACGGACAGGTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCTGTTGTTTATAGGGGTCATCAAA‑‑‑‑‑GAAATATTCTTCGAGTAAAGGGGCGATATCCGTCATCACAATTTCATTAAGCCAt                        >  2:759029/1‑139 (MQ=255)
                                                                                                             aGGTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCTGTTGTTTATAGGGGTCATCAAA‑‑‑‑‑GAAATAttc                                                                      >  2:971160/1‑86 (MQ=255)
                                                                                                             aGGTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCTGTTGTTTATAGGGGTCATCAAA‑‑‑‑‑GAAATAttc                                                                      <  1:971160/86‑1 (MQ=255)
                                                                                                             aGGTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCTGTTGTTTATAGGGGTCATCAAA‑‑‑‑‑GAAATATTCTTCGAGTAAAGGGGCGATATCCGTCATCACAATTTCATTAAGCCATTGc                     >  2:611648/1‑135 (MQ=255)
                                                                                                             aGGTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCTGTTGTTTATAGGGGTCATCAAA‑‑‑‑‑GAAATATTCTTCGAGTAAAGGGGCGATATCCGTCATCACAATTTCATTAAGCCATTGc                     <  1:611648/135‑1 (MQ=255)
                                                                                                               gTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCTGTTGTTTATAGGGGTCATCAAA‑‑‑‑‑GAAATATTCTTCGAGTAAAGGGGCGATATCCGTCATCACAATTTCATTAAGCCATTg                      >  2:849112/1‑132 (MQ=255)
                                                                                                               gTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCTGTTGTTTATAGGGGTCAACAAA‑‑‑‑‑GAAATATTCTTCGAGTAAAGGGGCGATATCCGTCATCACAATTTCATTAAGCCATTg                      <  1:849112/132‑1 (MQ=255)
                                                                                                                     cGGGCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCTGTTGTTTATAGGGGTCATCAAA‑‑‑‑‑GAAATATTCTTCGAGTAAAGGGGCGATATCCGTCATCACAATTTCATTAAGCCATTGCGTATCCGGagag         >  1:237829/1‑139 (MQ=255)
                                                                                                                         cTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCTGTTGTTTATAGGGGTCATCAAA‑‑‑‑‑GAAATATTCTTCGAGTAAAGGGGCGATATCCGTCATCACAATTTCATTAAGCCATTGCGTATCCGGAGAGGTGc     >  1:300024/1‑139 (MQ=255)
                                                                                                                            ttCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCTGTTGTTTATAGGGGTCATCAAA‑‑‑‑‑GAAATATTCTTCGAGTAAAGGGGCGATATCCGTCATCACAATTTCATTAAGCCATTGCGTATCCGGAGAGGTGcc    >  2:417986/1‑137 (MQ=255)
                                                                                                                            ttCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCTGTTGTTTATAGGGGTCATCAAA‑‑‑‑‑GAAATATTCTTCGAGTAAAGGGGCGATATCCGTCATCACAATTTCATTAAGCCATTGCGTATCCGGAGAGGTGcc    <  1:417986/137‑1 (MQ=255)
                                                                                                                            ttCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCTGTTGTTTATAGGGGTCATCAAA‑‑‑‑‑GAAATATTCTTCGAGTAAAGGGGCGATATCCGTCATCACAATTTCATTAAGCCATTGCGTATCCGGAGAGGTGCCAt  >  1:163061/1‑139 (MQ=255)
                                                                                                                                 |                                                                                                                                          
ACCCCAGGATATCAAGAAGATTGTTACCGGGTATGGCTTCAAGGTTTGCCTGCTTAATTTCCTGTAAATAACCCCATGCATAGGTAAGCATGTAATAGATATTACGGACAGGTATCACGGGCTGTTCCACTATGAGTCCCCTAATAATTTGTTGGTCCATTTCTGTTGTTTATAGGGGTCATCAAA‑‑‑‑‑GAAATATTCTTCGAGTAAAGGGGCGATATCCGTCATCACAATTTCATTAAGCCATTGCGTATCCGGAGAGGTGCCAT  >  NC_000913/4577829‑4578091

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: