Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,287,161 2 bp→AA noncoding (75‑76/171 nt)
coding (15‑16/90 nt)		 rttR ←
tpr ←		 rtT sRNA, processed from tyrT transcript
protamine‑like protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,287,1610GA100.0% 15.8 / NA 8noncoding (76/171 nt)
Q6* (CAA→TAA) 		rttR
tpr		rtT sRNA, processed from tyrT transcript
protamine‑like protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (3/5);  total (3/5)
*NC_0009131,287,1620GA100.0% 15.7 / NA 8noncoding (75/171 nt)
D5D (GAC→GAT) 		rttR
tpr		rtT sRNA, processed from tyrT transcript
protamine‑like protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (3/5);  total (3/5)

CAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGA  >  NC_000913/1287088‑1287282
                                                                         ||                                                                                                                        
cAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAATCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCa                                                          >  2:826450/1‑139 (MQ=255)
  gcgcTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAATCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAgg                                                        <  1:826450/139‑1 (MQ=255)
   cgcTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAATCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAggg                                                       <  2:187566/139‑1 (MQ=11)
                                ccTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAATCGAAGGTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGAGTCGTTGGGAAGTTCAGGGACTTTTGAAAGTgatggtggtgggggaag                          <  1:335284‑M2/139‑18 (MQ=255)
                                                     tgacgcTCGAGCCGAACCTTAATCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGa                                               <  1:764774/94‑1 (MQ=14)
                                                     tgacgcTCGAGCCGAACCTTAATCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGa                                               >  2:764774/4‑97 (MQ=14)
                                                                   aaCCTTAATCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTgatggtggtgggggaaggattactcagcgctgcgcgcttcg  >  1:443401‑M2/1‑87 (MQ=255)
                                                                   aaCCTTAATCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTgatggtggtgggggaaggattactcagcgctgcgcgcttcg  <  2:443401‑M2/128‑42 (MQ=255)
                                                                         ||                                                                                                                        
CAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGA  >  NC_000913/1287088‑1287282

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑