Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,763,987 Δ1 bp coding (23/1488 nt) sufB ← component of SufBCD Fe‑S cluster assembly scaffold

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,763,9870G.100.0% 176.9 / NA 42coding (23/1488 nt)sufBcomponent of SufBCD Fe‑S cluster assembly scaffold
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base . (17/25);  total (17/25)

TCATTACGCTTCGCCGAAATTGCGCGCACCACCTCTTCGTTTATCCCCTTTGCCAGCTCATCGGTGGCTAACTGGGTGAAGAATCCTTCTTTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCAGTTGCTTCAGTATTACGAGACATAGTACCGCCTATACCCCAAAGCTTTCGCCACAGCCACATTCATTCTGGGCTTTAGGGTTGTGAAATTTGAATATCTGATTAAGTCCTTCACGA  >  NC_000913/1763855‑1764102
                                                                                                                                    |                                                                                                                   
tCATTACGCTTCGCCGAACTTGCGCGCACCACCTCTTCGTTTATCCCCTTTGCCAGCTCATCGGTGGCTAACTGGGTGAAGAATCCTTCTTTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCATTGCTTc                                                                                                              <  1:747849/139‑1 (MQ=255)
         ttCGCCGAAATTGCGCGCACCACCTCTTCGTTTATCCCCTTTGCCAGCTCATCGGTGGCTAACTGGGTGAAGAATCCTTCTTTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCATTGCTTCAGTATTa                                                                                                       <  2:818146/137‑1 (MQ=255)
         ttCGCCGAAATTGCGCGCACCACCTCTTCGTTTATCCCCTTTGCCAGCTCATCGGTGGCTAACTGGGTGAAGAATCCTTCTTTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCATTGCTTCAGTATTa                                                                                                       >  1:818146/1‑137 (MQ=255)
         ttCGCCGAAATTGCGCGCACCACCTCTTCGTTTATCCCCTTTGCCAGCTCATCGGTGGCTAACTGGGTGAAGAATCCTTCTTTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCATTGCTTCAGTATTACg                                                                                                     <  1:795843/139‑1 (MQ=255)
         ttCGCCGAAATTGCGCGCACCACCTCTTCGTTTATCCCCTTTGCCAGCTCATCGGTGGCTAACTGGGTGAAGAATCCTTCTTTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCATTGCTTCAGTATTACg                                                                                                     <  1:1031708/139‑1 (MQ=255)
                aaaTTGCGCGCACCACCTCTTCGTTTATCCCCTTTGCCAGCTCATCGGTGGCTAACTGGGTGAAGAATCCTTCTTTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCATTGCTTCAGTATTACGAGACATa                                                                                              <  2:332975/139‑1 (MQ=255)
                  aTTGCGCGCACCACCTCTTCGTTTATCCCCTTTGCCAGCTCATCGGTGGCTAACTGGGTGAAGAATCCTTCTTTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCATTGCTTCAGTATTACGAGACATAGt                                                                                            <  1:315952/139‑1 (MQ=255)
                   ttGGGCGCACCCCCTCTTCGTTTATCCCCTTTGCCAGCTCATCGGTGGCTAACTGGGTGAAGAATCCTTCTTTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCATTGCTTCAGTATTACGAGACATAGTa                                                                                           <  1:722106/139‑1 (MQ=255)
                   ttGCGCGCACCACCTCTTCGTTTATCCCCTTTGCCAGCTCATCGGTGGCTAACTGGGTGAAGAATCCTTCTTTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCATTGCTTCAGTATTACGAGACATAGTa                                                                                           <  2:574615/139‑1 (MQ=255)
                                 tctTCGTTTATCCCCTTTGCCAGCTCATCGGTGGCTAACTGGGTGAAGAATCCTTCTTTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCATTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCa                                                                             <  2:76298/139‑1 (MQ=255)
                                       tttATCCCCTTTGCCAGCTCATCGGTGGCTAACTGGGTGAAGAATCCTTCTTTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCATTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAAGCtt                                                                       >  1:591348/1‑139 (MQ=255)
                                          aTCCCCTTTGCCAGCTCATCGGTGGCTAACTGGGTGAAGAATCCTTCTTTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCATTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAAGCTTTCg                                                                    >  2:239147/1‑139 (MQ=255)
                                             cccTTTGCCAGCTCATCGGTGGCTAACTGGGTGAAGAATCCTTCTTTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCATTGCTTc                                                                                                              >  1:309563/1‑94 (MQ=255)
                                             cccTTTGCCAGCTCATCGGTGGCTAACTGGGTGAAGAATCCTTCTTTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCATTGCTTc                                                                                                              <  2:309563/94‑1 (MQ=255)
                                               cTTTGCCAGCTCATCGGTGGCTAACTGGGTGAAGAATCCTTCTTTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCATTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAAGCTTTCgccaca                                                               <  1:239147/139‑1 (MQ=255)
                                                tttGCCAGCTCATCGGTGGCTAACTGGGTGAAGAATCCTTCTTTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCATTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAAGCTTTCgcc                                                                  <  1:20368/135‑1 (MQ=255)
                                                tttGCCAGCTCATCGGTGGCTAACTGGGTGAAGAATCCTTCTTTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCATTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAAGCTTTCgcc                                                                  >  2:20368/1‑135 (MQ=255)
                                                     cAGCTCATCGGTGGCTAACTGGGTGAAGAATCCTTCTTTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCATTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAAGCTTTCgccacagccaca                                                         >  1:508920/1‑139 (MQ=255)
                                                        cTCATCGGTGGCTAACTGGGTGAAGAATCCTTCTTTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCATTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAAGCTTTCGCCACAGCCAcattc                                                      >  2:250299/1‑139 (MQ=255)
                                                                         gggTGAAGAATCCTTCTTTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCATTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAAGCTTTCGCCACAGCCACATTCATTCTGGGCTTTAGGGt                                     <  2:591348/139‑1 (MQ=255)
                                                                              aagaaTCCTTCTTTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCATTGCTTCAg                                                                                                            >  1:213517/1‑63 (MQ=255)
                                                                              aagaaTCCTTCTTTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCATTGCTTCAg                                                                                                            <  2:213517/63‑1 (MQ=255)
                                                                               agaaTCCTTCTTTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCATTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAAGCTTTCGCCACAGCCACATTCATTCTGGGCTTTAGGGTTGTGaa                               <  2:478972/139‑1 (MQ=255)
                                                                                     cttcttTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCATTGCTTCAGTa                                                                                                          <  2:937642/58‑1 (MQ=255)
                                                                                     cttcttTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCATTGCTTCAGTa                                                                                                          >  1:937642/1‑58 (MQ=255)
                                                                                        cttTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCATTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAAGCTTTCgccacagc                                                             >  1:256291/1‑100 (MQ=255)
                                                                                        cttTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCATTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAAGCTTTCgccacagc                                                             <  2:256291/100‑1 (MQ=255)
                                                                                              aaTTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCATTGCTTCAGTATTACgaga                                                                                                  <  1:133114/57‑1 (MQ=255)
                                                                                              aaTTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCATTGCTTCAGTATTACgaga                                                                                                  >  2:133114/1‑57 (MQ=255)
                                                                                               aTTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCATTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAAGCTTTCGCCACAGCCACattcattc                                                  <  1:707142/104‑1 (MQ=255)
                                                                                               aTTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCATTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAAGCTTTCGCCACAGCCACattcattc                                                  >  2:707142/1‑104 (MQ=255)
                                                                                               aTTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCATTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAAGCTTTCGCCACAGCCACATTCATTCTGGGCTTTAGGGTTGTGAAATTTGAATATCTGAtt               <  1:250299/139‑1 (MQ=255)
                                                                                                     cGGGCCGCCGGTCCAGGTTTTGACATCGTCATTGCt                                                                                                                >  2:433531/1‑36 (MQ=255)
                                                                                                     cGGGCCGCCGGTCCAGGTTTTGACATCGTCATTGCt                                                                                                                <  1:433531/36‑1 (MQ=255)
                                                                                                     cGGGCCGCCGGTCCAGGTTTTGACATCGTCATTGCTTCAGTATTACg                                                                                                     >  2:27944/1‑47 (MQ=255)
                                                                                                     cGGGCCGCCGGTCCAGGTTTTGACATCGTCATTGCTTCAGTATTACg                                                                                                     <  1:27944/47‑1 (MQ=255)
                                                                                                     cGGGCCGCCGGTCCAGGTTTTGACATCGTCATTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAAGCTTTCgccacagc                                                             <  2:894982/87‑1 (MQ=255)
                                                                                                     cGGGCCGCCGGTCCAGGTTTTGACATCGTCATTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAAGCTTTCgccacagc                                                             >  1:894982/1‑87 (MQ=255)
                                                                                                     cGGGCCGCCGGTCCAGGTTTTGACATCGTCATTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAAGCTTTCGCCACAGCCACATTCATTCTg                                                <  2:9965/100‑1 (MQ=255)
                                                                                                     cGGGCCGCCGGTCCAGGTTTTGACATCGTCATTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAAGCTTTCGCCACAGCCACATTCATTCTg                                                >  1:9965/1‑100 (MQ=255)
                                                                                                     cGGGCCGCCGGTCCAGGTTTTGACATCGTCATTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAAGCTTTCGCCACAGCCACATTCATTCTGGGCTTTAGGGTTGTGAAATTTGAATATCTGATTAAGTcc         >  1:270088/1‑139 (MQ=255)
                                                                                                            ccgGTCCAGGTTTTGACATCGTCATTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAAGCTTTCGCCACAGCCACATTCATTCTGGGCTTTAGGGTTGTGAAATTTGAATATCTGATTAAGTCCTTCacga  <  2:270088/139‑1 (MQ=255)
                                                                                                                                    |                                                                                                                   
TCATTACGCTTCGCCGAAATTGCGCGCACCACCTCTTCGTTTATCCCCTTTGCCAGCTCATCGGTGGCTAACTGGGTGAAGAATCCTTCTTTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCAGTTGCTTCAGTATTACGAGACATAGTACCGCCTATACCCCAAAGCTTTCGCCACAGCCACATTCATTCTGGGCTTTAGGGTTGTGAAATTTGAATATCTGATTAAGTCCTTCACGA  >  NC_000913/1763855‑1764102

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: