Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,817,796 (T)6→5 coding (705/1353 nt) chbF ← phospho‑chitobiase, general 6‑phospho‑beta‑glucosidase activity

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,817,7910T.100.0% 246.3 / NA 58coding (710/1353 nt)chbFphospho‑chitobiase, general 6‑phospho‑beta‑glucosidase activity
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (28/30);  total (28/30)

GCTCGTGCGCCGCCTTTGTAGTATTCGCCCATTTCAATAGCCAGCATCTCTTTCTGCTTGAAGTAATACAGCAGATAAGAACATGGCAGCAGATTCAACGAACGAATTAAGCCCTCACTAAATGGCAGATCGAAAATATTTTTTACAGAGGACGCTTTTAACTGCCCTGACGCCACACCATCAAGCAATTCGGCAAAGCGCGACTTGCCATTTATCAGCACATCCTTAATGAACACCATATGGTTGAGGCCGAACAAATCGATAGATAAATC  >  NC_000913/1817653‑1817924
                                                                                                                                          |                                                                                                                                     
gcTCGTGCGCCGCCTTTGTAGTATTCGCCCATTTCAATAGCCAGCATCTCTTTCTGCTTGAAGTAATACAGCAGATAAGAACATGGCAGCGGATTCAACGAACGAATTAAGCCCTCACTAAATGGCAGATCGAAAatat                                                                                                                                       >  2:582453/1‑139 (MQ=255)
      gcgcCGCCTTTGTAGTATTCGCCCATTTCAATAGCCAGCATCTCTTTCTGCTTGAAGTAATACAGCAGATAAGAACATGGCAGCAGATTCAACGAACGAATTAAGCCCTCACTAAATGGCAGATCGAAAATAtttttac                                                                                                                                 >  2:712449/1‑137 (MQ=255)
           gccTTTGTAGTATTCGCCCATTTCAATAGCCAGCATCTCTTTCTGCTTGAAGTAATACAGCAGATAAGAACATGGCAGCGGATTCAACGAACGAATTAAGCCCTCACTAAATGGCAGATCGAAAATATTTTTACAGAgg                                                                                                                           <  1:582453/139‑1 (MQ=255)
             cTTTGTAGTATTCGCCCATTTCAATAGCCAGCATCTCTTTCTGCTTGAAGTAATACAGCAGATAAGAACATGGCAGCAGATTCAACGAACGAATTAAGCCCTCACTAAATGGCAGATCGAAAATATTTTTACAGAGGAc                                                                                                                         <  1:57281/139‑1 (MQ=255)
                       ttCGCCCATTTCAATAGCCAGCATCTCTTTCTGCTTGAAGTAATACAGCAGATAAGAACATGGCAGCAGATTCAACGAACGAATTAAGCCCTCACTAAATGGCAGATCGAAAATATTTTTACAGAGGACGCTTTTAACt                                                                                                               >  2:426403/1‑139 (MQ=255)
                         cGCCCATTTCAATAGCCAGCATCTCTTTCTGCTTGAAGTAATACAGCAGATAAGAACATGGCAGCAGATTCAACGAACGAATTAAGCCCTCACTAAATGGCAGATCGAAAatat                                                                                                                                       >  2:635805/1‑114 (MQ=255)
                         cGCCCATTTCAATAGCCAGCATCTCTTTCTGCTTGAAGTAATACAGCAGATAAGAACATGGCAGCAGATTCAACGAACGAATTAAGCCCTCACTAAATGGCAGATCGAAAatat                                                                                                                                       <  1:635805/114‑1 (MQ=255)
                          gCCCATTTCAATAGCCAGCATCTCTTTCTGCTTGAAGTAATACAGCAGATAAGAACATGGCAGCAGATTCAACGAACGAATTAAGCCCTCACTAAATGGCAGATCGAAAATATTTTTACAGAGGACGCTTTTAACTGcc                                                                                                            >  2:80028/1‑139 (MQ=255)
                            ccATTTCAATAGCCAGCATCTCTTTCTGCTTGAAGTAATACAGCAGATAAGAACATGGCAGCAGATTCAACGAACGAATTAAGCCCTCACTAAATGGCAGATCGAAAATATTTTTACAGAGGACGCTTTTAACTGCCCt                                                                                                          <  1:712449/139‑1 (MQ=255)
                                 tCAATAGCCAGCATCTCTTTCTGCTTGAAGTAATACAGCAGATAAGAACATGGCAGCAGATTCAACGAACGAATTAAGCCCTCACTAAATGGCAGATCGAAAATATTTTTACAGAGGACGCTTTTAACTGCACTGACGc                                                                                                     >  2:443007/1‑139 (MQ=255)
                                  cAATAGCCAGCATCTCTTTCTGCTTGAAGTAATACAGCAGATAAGAACATGGCAGCAGATTCAACGAACGAATTAAGCCCTCACTAAATGGCAGATCGAAAATATTTTTACAGAGGACGCTTTTAACTGCCCTGACGcc                                                                                                    <  2:929669/139‑1 (MQ=255)
                                     tAGCCAGCATCTCTTTCTGCTTGAAGTAATACAGCAGATAAGAACATGGCAGCAGATTCAACGAACGAATTAAGCCCTCACTAAATGGCAGATCGAAAATATTTTTACAGAGGACGCTTTTAACTGCCCTGACGCcaca                                                                                                 <  1:426403/139‑1 (MQ=255)
                                          aGCATCTCTTTCTGCTTGAAGTAATACAGCAGATAAGAACATGGCAGCAGATTCAACGAACGAATTAAGCCCTCACTAAATGGCAGATCGAAAATATTTTTACAGAGGACGCTTTTAACTGCCCTGACGCCACACCATc                                                                                            >  2:68934/1‑139 (MQ=255)
                                          aGCATCTCTTTCTGCTTGAAGTAATACAGCAGATAAGAACATGGCAGCAGATTCAACGAACGAATTAAGCCCTCACTAAATGGCAGATCGAAAATATTTTTACAGAGGACGCTTTTAACTGCCCTGACGCCACACCATc                                                                                            >  1:960875/1‑139 (MQ=255)
                                             aTCTCTTTCTGCTTGAAGTAATACAGCAGATAAGAACATGGCAGCAGATTCAACGAACGAATTAAGCCCTCACTAAATGGCAGATCGAAAATATTTTTACAGAGGACGCTTTTAACTGccc                                                                                                           >  2:658475/1‑121 (MQ=255)
                                             aTCTCTTTCTGCTTGAAGTAATACAGCAGATAAGAACATGGCAGCAGATTCAACGAACGAATTAAGCCCTCACTAAATGGCAGATCGAAAATATTTTTACAGAGGACGCTTTTAACTGccc                                                                                                           <  1:658475/121‑1 (MQ=255)
                                                         ttGAAGTAATACAGCAGATAAGAACATGGCAGCAGATTCAACGAACGAATTAAGCCCTCACTAAATGGCAGATCGAAAATATTTTTACAGAGGACGCTTTTAACTGCCCTGACGCCACACCATCAAGCAATTCGGCaaa                                                                             >  1:549205/1‑139 (MQ=255)
                                                                      gcagATAAGAACATGGCAGCAGATTCAACGAACGAATTAAGCCCTCACTAAATGGCTGATCGAAAATATTTTTACAGAGGACGCTTTTAACTGCCCTGACGCCACACCATCAAg                                                                                         >  1:752298/1‑114 (MQ=255)
                                                                      gcagATAAGAACATGGCAGCAGATTCAACGAACGAATTAAGCCCTCACTAAATGGCAGATCGAAAATATTTTTACAGAGGACGCTTTTAACTGCCCTGACGCCACACCATCAAg                                                                                         <  2:752298/114‑1 (MQ=255)
                                                                       cagATAAGAACATGGCAGCAGATTCAACGAACGAATTAAGCCCTCACTAAATGGCAGATCGAAAATATTTTTACAGAGGa                                                                                                                          <  2:293261/80‑1 (MQ=255)
                                                                       cagATAAGAACATGGCAGCAGATTCAACGAACGAATTAAGCCCTCACTAAATGGCAGATCGAAAATATTTTTACAGAGGa                                                                                                                          >  1:293261/1‑80 (MQ=255)
                                                                       cagATAAGAACATGGCAGCAGATTCAACGAACGAATTAAGCCCTCACTAAATGGCAGATCGAAAATATTTTTACAGAGGACGCTTTTAACTGCCCTGACGCca                                                                                                   <  1:350208/103‑1 (MQ=255)
                                                                       cagATAAGAACATGGCAGCAGATTCAACGAACGAATTAAGCCCTCACTAAATGGCAGATCGAAAATATTTTTACAGAGGACGCTTTTAACTGCCCTGACGCca                                                                                                   >  2:350208/1‑103 (MQ=255)
                                                                         gATAAGAACATGGCAGCAGATTCAACGAACGAATTAAGCCCTCACTAAATGGCAGATCGAAAATATTTTTACAGAGGACGCTTTTAACTGCCCTGACGCCACACCATCAAGCAATTCGGCAAAGCGCGACTTGCCAttt                                                             >  1:416200/1‑139 (MQ=255)
                                                                          aTAAGAACATGGCAGCAGATTCAACGAACGAATTAAGCCCTCACTAAATGGCAGATCGAAAATATTTTTACAGAGGACGCTTTTAACTGCCCTGACGCCACACCATCAAGCAATTCGGCAAAGCGCGACTTGCCATTTa                                                            <  2:1025989/139‑1 (MQ=255)
                                                                          aTAAGAACATGACAGCAGATTCAACGAACGAATTAAGCCCTCACTAAATGGCAGATCGAAAATATTTTTACAGAGGACGCTTTTAACTGCCCTGACGCCACACCATCAAGCAATTCGGCAAAGCGCGACTTGCCATTTa                                                            >  1:191722/1‑139 (MQ=255)
                                                                           tAAGAACATGGCAGCAGATTCAACGAACGAATTAAGCCCTCACTAAATGGCAGATCGAAAATATTTTTACAGAGGACGCTTTTAACTGCCCTGACGCCACACCATCAAGCAATTCGGCAAAGCGCGACTTGCCATTTAt                                                           <  2:416200/139‑1 (MQ=255)
                                                                                aCATGGCAGCAGATTCAACGAACGAATTAAGCCCTCACTAAATGGCAGATCGAAAATATTTTTACAGAGGACGCTTTTAACTGCCCTGACGCCACACCATCAAGCAATTCGGCAAAGCGCGACTTGCCATTTATCAGca                                                      <  2:960875/139‑1 (MQ=255)
                                                                                aCATGGCAGCAGATTCAACGAACGAATTAAGCCCTCACTAAATGGCAGATCGAAAATATTTTTACAGAGGACGCTTTTAACTGCCCTGACGCCACACCATCAAGCAATTCGGCAAAGCGCGACTTGCCATTTATCAGca                                                      <  1:345518/139‑1 (MQ=255)
                                                                                   tGGCAGCAGATTCAACGAACGAATTAAGCCCTCACTAAATGGCAGATCGAAAATATTTTTACAGAGGACGCTTTTAACTGCCCTGACGCCACACCATCAAGCAATTCGGCAAAGCGCGACTTGCCATTTATCAGca                                                      <  1:975366/136‑1 (MQ=255)
                                                                                   tGGCAGCAGATTCAACGAACGAATTAAGCCCTCACTAAATGGCAGATCGAAAATATTTTTACAGAGGACGCTTTTAACTGCCCTGACGCCACACCATCAAGCAATTCGGCAAAGCGCGACTTGCCATTTATCAGca                                                      >  2:975366/1‑136 (MQ=255)
                                                                                    ggCAGCAGATTCAACGAACGAATTAAGCCCTCACTAAATGGCAGATCGAAAATATTTTTACAGAGGACGCTTTTAACTGCCCTGACGCcaca                                                                                                 >  1:905052/1‑92 (MQ=255)
                                                                                    ggCAGCAGATTCAACGAACGAATTAAGCCCTCACTAAATGGCAGATCGAAAATATTTTTACAGAGGACGCTTTTAACTGCCCTGACGCcaca                                                                                                 <  2:905052/92‑1 (MQ=255)
                                                                                    ggCAGCAGATTCAACGAACGAATTAAGCCCTCACTAAATGGCAGATCGAAAATATTTTTACAGAGGACGCTTTTAACTGCCCTGACGCCACACCATCAAGCAATTCGGCAAAGCGCGACTTGCCATTTATCAGCACATc                                                  >  1:250430/1‑139 (MQ=255)
                                                                                    ggCAGCAGATTCAACGAACGAATTAAGCCCTCACTAAATGGCAGATCGAAAATATTTTTACAGAGGACGCTTTTAACTGCCCTGACGCCACACCATCAAGCAATTCGGCAAAGCGCGACTTGCCATTTATCAGCACATc                                                  >  1:974081/1‑139 (MQ=255)
                                                                                         cagATTCAACGAACGAATTAAGCCCTCACTAAATGGCAGATCGAAAATATTTTTACAGAgg                                                                                                                           >  2:517857/1‑61 (MQ=255)
                                                                                         cagATTCAACGAACGAATTAAGCCCTCACTAAATGGCAGATCGAAAATATTTTTACAGAgg                                                                                                                           <  1:517857/61‑1 (MQ=255)
                                                                                            aTTCAACGAACGAATTAAGCCCTCACTAAATGGCAGATCGAAAATATTTTTACAGAGGACGCTTTTAACTGCCCTGACGCCACACCATCAAGCAATTCGGCAAAGCGCGACTTGCCATTTATCAGCACATCCTTAATGa                                          >  1:12098/1‑139 (MQ=255)
                                                                                             ttCAACGAACGAATTAAGCCCTCACTAAATGGCAGATCGAAAATATTTTTACAGAGGACGCTTTTAACTGCCCTGACGCCACACCATCAAGCAATTCGGCAAAGCGCGACTTGCCATTTATCAGCACATCCTTAATGaa                                         <  1:68934/139‑1 (MQ=255)
                                                                                             ttCAACGAACGAATTAAGCCCTCACTAAATGGCAGATCGAAAATATTTTTACAGAGGACGCTTTTAACTGCCCTGACGCCACACCATCAAGCAATTCGGCAAAGCGCGACTTGCCATTTATCAGCACATCCTTAATGaa                                         <  1:80028/139‑1 (MQ=255)
                                                                                                   gaacgaaTTAAGCCCTCACTAAATGGCAGATCGAAAATATTTTTACAGAGGACGCTTTTAACTGCCCTGACGCCACACCATCAAGCAATTCGGCAAAGCGCGACTTGCCATTTATCAGCACATCCTTAATGAACACCat                                   <  1:443007/139‑1 (MQ=255)
                                                                                                             aGCCCTCACTAAATGGCAGATCGAAAATATTTTTACAGAGGACGCTTTTAACTGCCCTGACGCCACACCATCAAGCAATTCGGCAAAGCGCGACTTGCCATTTATCAGCACATCCTTAATGAACACCATATGGTTGAgg                         <  2:549205/139‑1 (MQ=255)
                                                                                                                   cACTAAATGGCAGATCGAAAATATTTTTACAGAGGACGCTTTTAACTGCCCTGACGCCACACCATCAAGCAATTCGGCAAAGCGCGACTTGCCATTTATCAGCACATcc                                                 <  1:1015088/109‑1 (MQ=255)
                                                                                                                   cACTAAATGGCAGATCGAAAATATTTTTACAGAGGACGCTTTTAACTGCCCTGACGCCACACCATCAAGCAATTCGGCAAAGCGCGACTTGCCATTTATCAGCACATcc                                                 >  2:1015088/1‑109 (MQ=255)
                                                                                                                         aTGGCAGATCGAAAATATTTTTACAGAGGACGCTTTTAACTGCCCTGACGCCACACCATCAAGCAATTCGGCAAAGCGCGACTTGCCATTTATCAGCACATCCTTAATGAACACCATATGGTTGAGGCCGAACAAATCg             <  2:191722/139‑1 (MQ=255)
                                                                                                                          tGGCAGATCGAAAATATTTTTACAGAGGACGCTTTTAACTGCCCTGACGCCACACCATCAAGCAATTCGGCAAAGCGCGACTTGCCATTTATCAGcac                                                     <  2:283717/98‑1 (MQ=255)
                                                                                                                          tGGCAGATCGAAAATATTTTTACAGAGGACGCTTTTAACTGCCCTGACGCCACACCATCAAGCAATTCGGCAAAGCGCGACTTGCCATTTATCAGcac                                                     >  1:283717/1‑98 (MQ=255)
                                                                                                                          tGGCAGATCGAAAATATTTTTACAGAGGACGCTTTTAACTGCCCTGACGCCACACCATCAAGCAATTCGGCAAAGCGCGACTTGCCATTTATCAGCACATCCTTAATGAACACCATATGGTTGAGGCCGAACAAATCga            <  1:976196/139‑1 (MQ=255)
                                                                                                                              aGATCGAAAATATTTTTACAGAGGACGCTTTTAACTGCCCTGACGCCACACCATCAAGCAATTCGGCAAAGCGCGACTTGCCATTTATCAGCACATCCTTAATGAACACCATATGGTTGAGGCCGAACAAATCgataga        >  1:985054/1‑139 (MQ=255)
                                                                                                                                aTCGAAAATATTTTTACAGAGGACGCTTTTAACTGCCCTGACGCCACACCATCAAGCAATTCGGCAAAGCGCGACTTGCCATTTATCAGCACATcc                                                 >  2:44942/1‑96 (MQ=255)
                                                                                                                                aTCGAAAATATTTTTACAGAGGACGCTTTTAACTGCCCTGACGCCACACCATCAAGCAATTCGGCAAAGCGCGACTTGCCATTTATCAGCACATcc                                                 <  1:44942/96‑1 (MQ=255)
                                                                                                                                aTCGAAAATATTTTTACAGAGGACGCTTTTAACTGCCCTGACGCCACACCATCAAGCAATTCGGCAAAGCGCGACTTGCCATTTATCAGCACATCCTTAAt                                            >  1:162827/1‑101 (MQ=255)
                                                                                                                                aTCGAAAATATTTTTACAGAGGACGCTTTTAACTGCCCTGACGCCACACCATCAAGCAATTCGGCAAAGCGCGACTTGCCATTTATCAGCACATCCTTAAt                                            <  2:162827/101‑1 (MQ=255)
                                                                                                                                aTCGAAAATATTTTTACAGAGGACGCTTTTAACTGCCCTGACGCCACACCATCAAGCAATTCGGCAAAGCGCGACTTGCCATTTATCAGCACATCCTTAATGaa                                         <  1:696106/104‑1 (MQ=255)
                                                                                                                                aTCGAAAATATTTTTACAGAGGACGCTTTTAACTGCCCTGACGCCACACCATCAAGCAATTCGGCAAAGCGCGACTTGCCATTTATCAGCACATCCTTAATGaa                                         >  2:696106/1‑104 (MQ=255)
                                                                                                                                aTCGAAAATATTTTTACAGAGGACGCTTTTAACTGCCCTGACGCCACACCATCAAGCAATTCGGCAAAGCGCGACTTGCCATTTATCAGCACATCCTTAATGAACACCATATGGt                              <  1:64584/115‑1 (MQ=255)
                                                                                                                                aTCGAAAATATTTTTACAGAGGACGCTTTTAACTGCCCTGACGCCACACCATCAAGCAATTCGGCAAAGCGCGACTTGCCATTTATCAGCACATCCTTAATGAACACCATATGGt                              >  2:64584/1‑115 (MQ=255)
                                                                                                                                aTCGAAAATATTTTTACAGAGGACGCTTTTAACTGCCCTGACGCCACACCATCAAGCAATTCGGCAAAGCGCGACTTGCCATTTATCAGCACATCCTTAATGAACACCATATGGTTGAGGCCGAACAAATCgatagata      >  1:243980/1‑139 (MQ=255)
                                                                                                                                aTCGAAAATATTTTTACAGAGGACGCTTTTAACTGCCCTGACGCCACACCATCAAGCAATTCGGCAAAGCGCGACTTGCCATTTATCAGCACATCCTTAATGAACACCATATGGTTGAGGCCGAACAAATCgatagata      <  2:985054/139‑1 (MQ=255)
                                                                                                                                  cGAAAATATTTTTACAGAGGACGCTTTTAACTGCCCTGACGCCACACCATCAAGCAATTCGGCAAAGCGCGACTTGc                                                                  <  2:731644/77‑1 (MQ=255)
                                                                                                                                  cGAAAATATTTTTACAGAGGACGCTTTTAACTGCCCTGACGCCACACCATCAAGCAATTCGGCAAAGCGCGACTTGc                                                                  >  1:731644/1‑77 (MQ=255)
                                                                                                                                    aaaaTATTTTTACAGAGGACGCTTTTAACTGCCCTGACGCCACACCATCAAGCAATTCGGCAAAGCGCGACTTGCCATTTATCAGCACATCCTTAATGAACACCATATGGTTGAGGCCGAACAAATCGATAGATAAatc  >  1:647809/1‑139 (MQ=255)
                                                                                                                                          |                                                                                                                                     
GCTCGTGCGCCGCCTTTGTAGTATTCGCCCATTTCAATAGCCAGCATCTCTTTCTGCTTGAAGTAATACAGCAGATAAGAACATGGCAGCAGATTCAACGAACGAATTAAGCCCTCACTAAATGGCAGATCGAAAATATTTTTTACAGAGGACGCTTTTAACTGCCCTGACGCCACACCATCAAGCAATTCGGCAAAGCGCGACTTGCCATTTATCAGCACATCCTTAATGAACACCATATGGTTGAGGCCGAACAAATCGATAGATAAATC  >  NC_000913/1817653‑1817924

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: