Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,875,056 (C)6→5 coding (521/822 nt) yeaM ← putative DNA‑binding transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,875,0510C.100.0% 167.6 / NA 40coding (526/822 nt)yeaMputative DNA‑binding transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base . (19/21);  total (19/21)

CCTGAAGCGCCATAATCAGCTGTAACTGCTGACGCCACTGGCGAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGCCCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGAAACGGGCAGGTGCAGCTGTTGTTGTGGCTGCTGAGGGAGTTCATCAAACAGGACCTGAATTAATCT  >  NC_000913/1874925‑1875180
                                                                                                                              |                                                                                                                                 
ccTGAAGCGCCATAATCAGCTGTAACTGCTGACGCCACTGGCGAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGCCCCCACTCGACa                                                                                                                      >  1:577090/1‑139 (MQ=255)
ccTGAAGCGCCATAATCAGCTGTAACTGCTGACGCCACTGGCGAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGCCCCCACTCGACa                                                                                                                      <  1:966743/139‑1 (MQ=255)
       cgcCATAATCAGCTGTAACTGCTGACGCCACTGGCGAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGCCCCCACTCGACAGGCCCtt                                                                                                               <  1:940862/139‑1 (MQ=255)
          cATAATCAGCTGTAACTGCTGACGCCACTGGCGAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGCCCCCACTCGACAGGCCCTTTCg                                                                                                            >  2:684406/1‑139 (MQ=255)
            tAATCAGCTGTAACTGCTGACGCCACTGGCGAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGCCCCCACTCGACAGGCCCTTTCGcc                                                                                                          <  2:740424/139‑1 (MQ=255)
            tAATCAGCTGTAACTGCTGACGCCACTGGCGAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGCCCCCACTCGACAGGCCCTTTCGcc                                                                                                          <  2:528282/139‑1 (MQ=255)
                    tGTAACTGCTGACGCCACTAGCGAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATG‑‑CCCCACTCGACAGGCCCTTTCGCCATCATCTcc                                                                                                 >  2:389645/1‑139 (MQ=255)
                           gctgACGCCACTGGCGAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGCCCCCACTCGACAGGCCCTTTCGCca                                                                                                         >  1:691814/1‑125 (MQ=255)
                           gctgACGCCACTGGCGAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGCCCCCACTCGACAGGCCCTTTCGCca                                                                                                         <  2:691814/125‑1 (MQ=255)
                           gctgACGCCACTGGCGAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGCCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATc                                                                                           <  1:684406/139‑1 (MQ=255)
                             tgACGCCACTGGCGATAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGCCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGt                                                                                         >  2:743861/1‑139 (MQ=255)
                               aCGCCACTGGCGAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGCCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGc                                                                                       <  1:144055/139‑1 (MQ=255)
                                    aCTGGCGAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGCCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGAtt                                                                                  >  1:384187/1‑139 (MQ=255)
                                     cTGGCGAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGCCCCCACTCGACAGGCCCTTTCGCca                                                                                                         >  2:639060/1‑115 (MQ=255)
                                     cTGGCGAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGCCCCCACTCGACAGGCCCTTTCGCca                                                                                                         <  1:639060/115‑1 (MQ=255)
                                                    ccccGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGCCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGaaa                                                                  <  2:577090/139‑1 (MQ=255)
                                                        gTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGCCCCCACTCGACAGGCCCTTTCGCcatc                                                                                                       >  1:933664/1‑98 (MQ=255)
                                                        gTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGCCCCCACTCGACAGGCCCTTTCGCcatc                                                                                                       <  2:933664/98‑1 (MQ=255)
                                                                 aCTATCAGTCGGGCAAGATTGCATTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGCCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGAAACGGGCAGGTGCAg                                                     <  1:658523/139‑1 (MQ=255)
                                                                   tATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATG‑‑CCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGAAACGGGCAGGTGCAGCtg                                                  <  1:389645/139‑1 (MQ=255)
                                                                     tCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGCCCCCACTCGACAGGCCCTTTCGCcatc                                                                                                       <  1:507787/85‑1 (MQ=255)
                                                                     tCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGCCCCCACTCGACAGGCCCTTTCGCcatc                                                                                                       >  2:507787/1‑85 (MQ=255)
                                                                       aGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGCCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATgag                                                                        <  2:157048/114‑1 (MQ=255)
                                                                       aGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGCCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATgag                                                                        >  1:157048/1‑114 (MQ=255)
                                                                       aGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGCCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGAAACGGGCAGGTGc                                                       >  2:1087400/1‑131 (MQ=255)
                                                                       aGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGCCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGAAACGGGCAGGTGc                                                       <  1:1087400/131‑1 (MQ=255)
                                                                       aGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGCCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGAAACGGGCAGGTGCAGCtgttg                                               >  1:414762/1‑139 (MQ=255)
                                                                       aGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGCCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGAAACGGGCAGGTGCAGCtgttg                                               >  2:45571/1‑139 (MQ=255)
                                                                         tCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGCCCCCACTCGACAgg                                                                                                                    <  1:625184/68‑1 (MQ=255)
                                                                         tCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGCCCCCACTCGACAgg                                                                                                                    >  2:625184/1‑68 (MQ=255)
                                                                            ggCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGCCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGAAACGGGCAGGTGCAGCTGTTGTtgtg                                          <  1:45571/139‑1 (MQ=255)
                                                                             gCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGCCCCCACTCGACAGGCCCTTTCGCcatc                                                                                                       >  2:611877/1‑77 (MQ=255)
                                                                             gCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGCCCCCACTCGACAGGCCCTTTCGCcatc                                                                                                       <  1:611877/77‑1 (MQ=255)
                                                                                      cGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGc                                                                                                                                   <  2:162273/41‑1 (MQ=255)
                                                                                      cGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGc                                                                                                                                   >  1:162273/1‑41 (MQ=255)
                                                                                      cGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGCCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGaaa                                                                  <  2:230592/105‑1 (MQ=255)
                                                                                      cGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGCCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGaaa                                                                  >  1:230592/1‑105 (MQ=255)
                                                                                                 cGCAAAAAAGCCAGCCCATTGCCCCAATGCCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGAAACGGGCAGGTGCAGCTGTTGTTGTGGCTGCTGAGGGAGTTCATCaa                     >  1:1008830/1‑139 (MQ=255)
                                                                                                 cGCAAAAAAGCCAGCCCATTGCCCCAATGCCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGAAACGGGCAGGTGCAGCTGTTGTTGTGGCTGCTGAGGGAGTTCATCaa                     >  1:837905/1‑139 (MQ=255)
                                                                                                       aaaGCCAGCCCATTGCCCCAATGCCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGAAACGGGCAGGTGCAGCTGTTGTTGTGGCTGCTGAGGGAGTTCATCAAACAGGa               <  1:35420/139‑1 (MQ=255)
                                                                                                           ccagccCATTGCCCCAATGCCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGAAACGGGCAGGTGCAGCTGTTGTTGTGGCTGCTGAGGGAGTTCATCAAACAGGACCTg           >  2:394619/1‑139 (MQ=255)
                                                                                                                    tGCCCCAATGCCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGAAACGGGCAGGTGCAGCTGTTGTTGTGGCTGCTGAGGGAGTTCATCAAACAGGACCTGAATTAATCt  <  2:384187/139‑1 (MQ=255)
                                                                                                                              |                                                                                                                                 
CCTGAAGCGCCATAATCAGCTGTAACTGCTGACGCCACTGGCGAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGCCCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGAAACGGGCAGGTGCAGCTGTTGTTGTGGCTGCTGAGGGAGTTCATCAAACAGGACCTGAATTAATCT  >  NC_000913/1874925‑1875180

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: