Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,214,339 (C)6→5 coding (306/1686 nt) yehU ← inner membrane putative sensory kinase in two‑component system with YehT

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,214,3340C.100.0% 198.4 / NA 47coding (311/1686 nt)yehUinner membrane putative sensory kinase in two‑component system with YehT
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base . (25/22);  total (25/22)

GCGACGAACGTGACGGCACCGGCGGTAATGGGGTTAAAGACTTTATCAGTGCGCCCGCGGCGGATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGCCCCCCATCGAATATC‑‑‑GATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTATCGCACGGGTATTGGCAATAGAATCGTCAATGTGCAACCCAAAC  >  NC_000913/2214196‑2214468
                                                                                                                                          |                                                                                                                                         
gCGACGAACGTGACGGCACCGGCGGTAATGGGGTTAAAGACTTTATCAGTGCGCCCGCGGCGGATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGc                                                                                                                                           <  1:1097597/139‑1 (MQ=255)
       aCGTGACGGCACCGGCGGTAATGGGGTTAAAGACTTTATCAGTGCGCCCGCGGCGGATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGcccccatc                                                                                                                                    <  2:387840/139‑4 (MQ=255)
             cGGCACCGGCGGTAATGGGGTTAAAGACTTTATCAGTGCGCCCGCGGCGGATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGCCCCCATCGAatat                                                                                                                             <  1:843532/139‑1 (MQ=255)
                            tGGGGTTAAAGACTTTATCAGTGCGCCCGCGGCGGATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGCCCCCATCGAATATC‑‑‑GATGTAAGccgccg                                                                                                           <  1:621701/139‑1 (MQ=255)
                                        cTTTATCAGTGCGTCCGCGGCGGATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGccc                                                                                                                                         <  1:641043/101‑1 (MQ=255)
                                        cTTTATCAGTGCGCCCGCGGCGGATCAGGGTGCTGTGTACCAGGCTACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGccc                                                                                                                                         >  2:641043/1‑101 (MQ=255)
                                           tATCAGTGCGCCCGCGGCGGATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGCCCCCATCGAATATC‑‑‑GATGTAAGCCGCCGGTCAGCCCAACCAGc                                                                                            >  2:288538/1‑139 (MQ=255)
                                                           gcggATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGCCCCCATCGAATATC‑‑‑GATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACgaccggac                                                                            >  2:143991/1‑139 (MQ=255)
                                                                    gATGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGCCCCCATCGAATATC‑‑‑GATGTAAgc                                                                                                                <  1:295173/94‑1 (MQ=255)
                                                                    gATGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGCCCCCATCGAATATC‑‑‑GATGTAAgc                                                                                                                >  2:295173/1‑94 (MQ=255)
                                                                      tGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGCCCCCATCGAATATC‑‑‑GATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACgaccgga                                                                             <  2:564684/127‑1 (MQ=255)
                                                                      tGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGCCCCCATCGAATATC‑‑‑GATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACgaccgga                                                                             >  1:564684/1‑127 (MQ=255)
                                                                      tGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGCCCCCATCGAATATC‑‑‑GATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAg                                                                 <  1:288538/139‑1 (MQ=255)
                                                                         tgtgTACCAGGCCACCGAGTAATACTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGCCCCCATCGAATATC‑‑‑GATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAgccg                                                              >  2:1067062/1‑139 (MQ=255)
                                                                                 aGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGCCCCCATCGAATATC‑‑‑GATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTAc                                                      <  1:143991/139‑1 (MQ=255)
                                                                                 aGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGCCCCCATCGAATATC‑‑‑GATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTAc                                                      >  2:1087730/1‑139 (MQ=255)
                                                                                  ggCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGCCCCCATCGAATATC‑‑‑GATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTAcc                                                     >  2:43471/1‑139 (MQ=255)
                                                                                  ggCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGCCCCCATCGAATATC‑‑‑GATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTAcc                                                     >  1:650283/1‑139 (MQ=255)
                                                                                  ggCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGCCCCCATCGAATATC‑‑‑GATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTAcc                                                     >  1:388943/1‑139 (MQ=255)
                                                                                   gCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGCCCCCATCGAATATC‑‑‑GATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACcg                                                    >  2:946815/1‑139 (MQ=255)
                                                                                     cACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGCCCCCATCGAATATC‑‑‑GATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACcgcg                                                  >  2:821063/1‑139 (MQ=255)
                                                                                       ccGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGccc                                                                                                                                         <  2:133999/54‑1 (MQ=255)
                                                                                       ccGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGccc                                                                                                                                         >  1:133999/1‑54 (MQ=255)
                                                                                       ccGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGCCCCCATCGAATATC‑‑‑GATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACgaccgga                                                                             >  1:1053925/1‑110 (MQ=255)
                                                                                       ccGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGCCCCCATCGAATATC‑‑‑GATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACgaccgga                                                                             <  2:1053925/110‑1 (MQ=255)
                                                                                       ccGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGCCCCCATCGAATATC‑‑‑GATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGcc                                                >  1:604062/1‑139 (MQ=255)
                                                                                       ccGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGCCCCCATCGAATATC‑‑‑GATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGcc                                                <  1:43471/139‑1 (MQ=255)
                                                                                        cGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGCCCCCATCGAATATC‑‑‑GATGTa                                                                                                                   <  2:307997/71‑1 (MQ=255)
                                                                                        cGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGCCCCCATCGAATATC‑‑‑GATGTa                                                                                                                   >  1:307997/1‑71 (MQ=255)
                                                                                            tAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGCCCCCATCGAATATC‑‑‑GATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTATCg                                           <  1:1067062/139‑1 (MQ=255)
                                                                                             aaTCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGCCCCCATCGAATATC‑‑‑GATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAgccgcc                                                            <  2:669428/121‑1 (MQ=255)
                                                                                             aaTCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGCCCCCATCGAATATC‑‑‑GATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAgccgcc                                                            >  1:669428/1‑121 (MQ=255)
                                                                                              aTCCTTCAACGATGGTCGAGATCATGCAGCTTAACGCGGTCATGCCCCCATCGAATATC‑‑‑GATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTATCGCa                                         <  1:455739/139‑1 (MQ=255)
                                                                                              aTCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGCCCCCATCGAATATC‑‑‑GATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTATCGCa                                         <  2:243452/139‑1 (MQ=255)
                                                                                                   tCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGCCCCCATCGAATATC‑‑‑GATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTATCGCACGGGt                                    <  1:891981/139‑1 (MQ=255)
                                                                                                          tGGTCGAGATCATGCGACTTAACGCGGTCATGCCCCCATCGAATATC‑‑‑GATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTATCGCACGGGTATTGGCa                             >  1:787406/1‑139 (MQ=255)
                                                                                                          tGGTCGAGATCATGCAACTTAACGCGGTCATGCCCCCATCGAATATC‑‑‑GATGTAAGCCGCCgg                                                                                                          >  1:275197/1‑62 (MQ=255)
                                                                                                          tGGTCGAGATCATGCAACTTAACGCGGTCATGCCCCCATCGAATATC‑‑‑GATGTAAGCCGCCgg                                                                                                          <  2:275197/62‑1 (MQ=255)
                                                                                                          tGGTCGAGATCATGCAACTTAACGCGGTCATGCCCCCATCGAATATC‑‑‑GATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTATCGCACGGGTATTGGCa                             <  1:946815/139‑1 (MQ=255)
                                                                                                             tCGAGATCATGCAACTTAACGCGGTCATGCCCCCATCGAATATC‑‑‑GATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTATCGCACGGGTATTGGCAATa                          >  1:298395/1‑139 (MQ=255)
                                                                                                                agaTCATGCAACTTAACGCGGTCATGCCCCCATCGAATATC‑‑‑GATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTATCGCACGGGTATTGGCAATAGaa                       <  2:604062/139‑1 (MQ=255)
                                                                                                                 gaTCATGCAACTTAACGCGGTCATGCCCCCATCGAATATC‑‑‑GATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTATCGCACGGGTATTGGCAATAGAAt                      <  1:821063/139‑1 (MQ=255)
                                                                                                                      tGCAACTTAACGCGGTCATGCCCCCATCGAATATC‑‑‑GATGTAAGCCGCCGGTCAGCCCAAc                                                                                                >  2:186072/1‑60 (MQ=255)
                                                                                                                      tGCAACTTAACGCGGTCATGCCCCCATCGAATATC‑‑‑GATGTAAGCCGCCGGTCAGCCCAAc                                                                                                <  1:186072/60‑1 (MQ=255)
                                                                                                                      tGCAACTTAACGCGGTCATGCCCCCATCGAATATC‑‑‑GATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTATCGCACGGGTATTGGCAATAGAATCGTCa                 >  2:337482/1‑139 (MQ=255)
                                                                                                                      tGCAACTTAACGCGGTCATGCCCCCATCGAATATC‑‑‑GATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTATCGCACGGGTATTGGCAATAGAATCGTCa                 >  1:410322/1‑139 (MQ=255)
                                                                                                                       gCAACTTAACGCGGTCATGCCCCCATCGAATATCGATGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTATCGCa                                         <  2:931226/117‑1 (MQ=255)
                                                                                                                       gCAACTTAACGCGGTCATGCCCCCATCGAATATCGATGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTATCGCa                                         >  1:931226/1‑117 (MQ=255)
                                                                                                                           cTTAACGCGGTCATGCCCCCATCGAATATC‑‑‑GATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTATCGCACGGGTATTGGCAATAGAATCGTCAAtgtg            <  1:337482/139‑1 (MQ=255)
                                                                                                                           cTTAACGCGGTCATGCCCCCATCGAATATC‑‑‑GATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTATCGCACGGGTATTGGCAATAGAATCGTCAAtgtg            <  2:733299/139‑1 (MQ=255)
                                                                                                                              aaCGCGGTCATGCCCCCATCGAATATC‑‑‑GATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTATCGCACGGGTATTGGCAATAGAATCGTCAATGTGCaa         >  1:625512/1‑139 (MQ=255)
                                                                                                                              aaCGCGGTCATGCCCCCATCGAATATC‑‑‑GATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTATCGCACGGGTATTGGCAATAGAATCGTCAATGTGCaa         >  1:803147/1‑139 (MQ=255)
                                                                                                                                     tCATGCCCCCATCGAATATC‑‑‑GATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTATCGCACGGGTATTGGCAATAGAATCGTCAATGTGCAACCCAAAc  >  1:161912/1‑139 (MQ=255)
                                                                                                                                          |                                                                                                                                         
GCGACGAACGTGACGGCACCGGCGGTAATGGGGTTAAAGACTTTATCAGTGCGCCCGCGGCGGATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGCCCCCCATCGAATATC‑‑‑GATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTATCGCACGGGTATTGGCAATAGAATCGTCAATGTGCAACCCAAAC  >  NC_000913/2214196‑2214468

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: