Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,462,889 (C)9→8 intergenic (+243/‑123) fadL → / → yfdF long‑chain fatty acid outer membrane transporter/uncharacterized protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,462,8810C.100.0% 115.3 / NA 28intergenic (+235/‑131)fadL/yfdFlong‑chain fatty acid outer membrane transporter/uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base . (11/17);  total (11/17)

CAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTGTAACAAATCAATCAAAATGGAATAAA  >  NC_000913/2462746‑2463008
                                                                                                                                       |                                                                                                                               
cAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGGCAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCCGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTcccc                                                                                                                              <  1:432592/139‑1 (MQ=255)
  aaCAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGGCAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCCGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTcccccc                                                                                                                            <  1:377230/139‑1 (MQ=255)
  aaCAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGGCAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCCGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTcccccc                                                                                                                            <  2:11899/139‑1 (MQ=255)
          gTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGGCAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCCGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCTGGCTa                                                                                                                   <  2:22473/139‑1 (MQ=255)
           ttGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGGCAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCCGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCTGGCTAt                                                                                                                  >  1:938184/1‑139 (MQ=255)
                           tCAAATCAATAAGCGGTATCCACAGGCAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCCGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCTGGCTATtgtg                                                                                                              >  1:177865/1‑127 (MQ=255)
                           tCAAATCAATAAGCGGTATCCACAGGCAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCCGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCTGGCTATtgtg                                                                                                              <  2:177865/127‑1 (MQ=255)
                            cAAATCAATAAGCGGTATCCACAGGCAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCCGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCTGGCTATTGTGCGCTCATACACTc                                                                                                 <  2:721080/139‑1 (MQ=255)
                                          gTATCCACAGGCAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCCGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCTGGCTATTGTgcg                                                                                                            >  1:295896/1‑114 (MQ=255)
                                          gTATCCACAGGCAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCCGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCTGGCTATTGTgcg                                                                                                            <  2:295896/114‑1 (MQ=255)
                                            aTCCACAGGCAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCCGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGtt                                                                                 <  2:587196/139‑1 (MQ=255)
                                                  aggcAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCCGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCtaaat                                                                           >  1:1086423/4‑139 (MQ=255)
                                                    gcAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCCGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGAt                                                                                      >  1:346275/2‑126 (MQ=255)
                                                    gcAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCCGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGAt                                                                                      <  2:346275/125‑1 (MQ=255)
                                                     cAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCCGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGtt                                                                                 <  1:1015530/130‑1 (MQ=255)
                                                     cAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCCGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGtt                                                                                 >  2:1015530/1‑130 (MQ=255)
                                                         ccTGAAGGGAAAGGCATATTTTCAGGCGTTCCGCTCGTCCTTCTCAAAGATTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAg                                                                    <  2:680011/139‑1 (MQ=255)
                                                         ccTGAAGGGAAAGGCATATTTTCAGGCGTTCCGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAg                                                                    <  2:1086423/139‑1 (MQ=255)
                                                           tGAAGGGAAAGGCATATTTTCAGGCGTTCCGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTccccccc                                                                                                                           <  1:1057945/83‑1 (MQ=255)
                                                           tGAAGGGAAAGGCATATTTTCAGGCGTTCCGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTccccccc                                                                                                                           >  2:1057945/1‑83 (MQ=255)
                                                              atggAAAGGCATATTTTCAGGCGTTCCGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTcccccc                                                                                                                            <  2:704230/77‑1 (MQ=255)
                                                              aGGGAAAGGCATATTTTCAGGCGTTCCGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTcccccc                                                                                                                            >  1:704230/1‑79 (MQ=255)
                                                                   aaGGCATATTTTCAGGCGTTCCGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGa                                                          <  2:207054/139‑1 (MQ=255)
                                                                   aaGGCATATTTTCAGGCGTTCCGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGa                                                          <  1:439445/139‑1 (MQ=255)
                                                                   aaGGCATATTTTCAGGCGTTCCGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGa                                                          <  1:195452/139‑1 (MQ=255)
                                                                   aaGGCATATTTTCAGGCGTTCCGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGa                                                          <  2:938184/139‑1 (MQ=255)
                                                                      gCATATTTTCAGGCGTTCCGCTCGTCCTTCTTAAAGAGGTTATTTGTGTGAATTGACAGGATACTCCCCCCCCTGGCTATTGTGCGCTCAAACACTCAAAGTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAg                                                       <  2:987215/139‑1 (MQ=255)
                                                                                aGGCGTTCCGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCAt                                             >  1:858575/1‑139 (MQ=255)
                                                                                 ggCGTTCCGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCAtt                                            <  2:981921/139‑1 (MQ=255)
                                                                                     ttCCGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGt                                        >  1:745206/1‑139 (MQ=255)
                                                                                                           gTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCTGGCTATTGTGCGCTCa                                                                                                        >  2:210192/1‑53 (MQ=255)
                                                                                                           gTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCTGGCTATTGTGCGCTCa                                                                                                        <  1:210192/53‑1 (MQ=255)
                                                                                                                           ttCCAGGATACTCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTGTAACAAATCAATCAAAATGGAATaaa  >  1:933752/1‑139 (MQ=255)
                                                                                                                             ccAGGATACTCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCa                                              <  1:511168/93‑1 (MQ=255)
                                                                                                                             ccAGGATACTCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCa                                              >  2:511168/1‑93 (MQ=255)
                                                                                                                                       |                                                                                                                               
CAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTGTAACAAATCAATCAAAATGGAATAAA  >  NC_000913/2462746‑2463008

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: