Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,765,829 (C)5→4 intergenic (‑53/‑89) yfjM ← / → rnlA CP4‑57 prophage, uncharacterized protein/CP4‑57 prophage, RNase LS

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,765,8250C.100.0% 171.1 / NA 41intergenic (‑49/‑93)yfjM/rnlACP4‑57 prophage, uncharacterized protein/CP4‑57 prophage, RNase LS
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base . (19/22);  total (19/22)

TGTCTGCTGGCGAATAATTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGAAATGACAATCAGGAGTTACAAAAACTTAAATCTGGTCAGGGCA  >  NC_000913/2765689‑2765959
                                                                                                                                         |                                                                                                                                      
tGTCTGCTGGCGAATAATTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGcc                                                                                                                                       >  2:868003/1‑139 (MQ=255)
                              aGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCTATTCAGGTGGCTAAATTTAGCAAATCa                                                                                                        >  1:493760/1‑139 (MQ=255)
                                 tgctgcTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATa                                                                                                     <  1:374509/139‑1 (MQ=255)
                                 tgctgcTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATa                                                                                                     <  2:544764/139‑1 (MQ=255)
                                        tGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCa                                                                                              >  2:842779/1‑139 (MQ=255)
                                         ggTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAg                                                                                             <  1:868003/139‑1 (MQ=255)
                                           tGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAggg                                                                                           <  2:493760/139‑1 (MQ=255)
                                           tGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAggg                                                                                           <  1:909976/139‑1 (MQ=255)
                                                          gTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCCGGTAGACGATCCTGCTCTATAGTTGGAGTGCCCCTATTCAGGTGGCTAAATTTAGCAAAt                                                                                                          >  1:980903/1‑109 (MQ=255)
                                                          gTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCTATTCAGGTGGCTAAATTTAGCAAAt                                                                                                          <  2:980903/109‑1 (MQ=255)
                                                            tACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCTATTCAGGTGGCTAAATTTAGCAAAt                                                                                                          <  1:57694/107‑1 (MQ=255)
                                                            tACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCTATTCAGGTGGCTAAATTTAGCAAAt                                                                                                          >  2:57694/1‑107 (MQ=255)
                                                            tACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGt                                                                          <  1:385576/139‑1 (MQ=255)
                                                                gAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTcc                                                                      <  2:372912/139‑1 (MQ=255)
                                                                             tcatcCCCCATTTTGTGGACTGCCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTa                                                         <  1:842779/139‑1 (MQ=255)
                                                                              catcCCCCATTTTGTGGACTGCCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAggg                                                                                           >  2:435397/1‑104 (MQ=255)
                                                                              catcCCCCATTTTGTGGACTGCCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAggg                                                                                           <  1:435397/104‑1 (MQ=255)
                                                                              catcCCCCATTTTGTGGACTGCCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTAt                                                        >  2:684413/1‑139 (MQ=255)
                                                                               atcCCCCATTTTGTGGACTGCCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATaca                                                                                                   >  1:951666/1‑95 (MQ=255)
                                                                               atcCCCCATTTTGTGGACTGCCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATaca                                                                                                   <  2:951666/95‑1 (MQ=255)
                                                                                 cccccATTTTGTGGACTGCCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCTATTCAGGTGGCTAAATTTAg                                                                                                               >  2:729428/1‑81 (MQ=255)
                                                                                 cccccATTTTGTGGACTGCCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCTATTCAGGTGGCTAAATTTAg                                                                                                               <  1:729428/81‑1 (MQ=255)
                                                                                 cccccATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCa                                                                   >  2:547229/1‑124 (MQ=255)
                                                                                 cccccATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCa                                                                   <  1:547229/124‑1 (MQ=255)
                                                                                            tgGACTGCCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCTATTCAGGTGGCTAAATTTAg                                                                                                               <  2:113552/70‑1 (MQ=255)
                                                                                            tgGACTGCCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCTATTCAGGTGGCTAAATTTAg                                                                                                               >  1:113552/1‑70 (MQ=255)
                                                                                                 tgccccccccGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGAAATGACaa                                     >  1:320810/4‑139 (MQ=255)
                                                                                                 tgccccccccGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGAAATGACaa                                     >  1:851415/4‑139 (MQ=255)
                                                                                                   ccccccccGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGAAATGACAATc                                   >  2:589016/2‑139 (MQ=255)
                                                                                                                              taGTTGGAGTGCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAgggggg                                                                                        <  1:715962/59‑1 (MQ=255)
                                                                                                                              taGTTGGAGTGCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAgg                                                                                            >  1:760144/1‑55 (MQ=255)
                                                                                                                              taGTTGGAGTGCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAgg                                                                                            <  2:760144/55‑1 (MQ=255)
                                                                                                                              taGTTGGAGTGCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTcc                                                                      >  1:964557/1‑77 (MQ=255)
                                                                                                                              taGTTGGAGTGCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTcc                                                                      <  2:964557/77‑1 (MQ=255)
                                                                                                                              taGATGGAGTGCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAgggggg                                                                                        >  2:715962/1‑59 (MQ=255)
                                                                                                                               aGTTGGAGTGCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAg                                                                                             <  2:730442/53‑1 (MQ=255)
                                                                                                                               aGTTGGAGTGCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAg                                                                                             >  1:730442/1‑53 (MQ=255)
                                                                                                                                   ggAGTGCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATaca                                                                                                   >  2:411765/1‑43 (MQ=255)
                                                                                                                                   ggAGTGCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATaca                                                                                                   <  1:411765/43‑1 (MQ=255)
                                                                                                                                   ggAGTGCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGAAATGACAATCAGGAGTTACAAAAACTTAAATCTGGTc        >  1:947920/1‑134 (MQ=255)
                                                                                                                                   ggAGTGCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGAAATGACAATCAGGAGTTACAAAAACTTAAATCTGGTc        <  2:947920/134‑1 (MQ=255)
                                                                                                                                    gAGTGCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGAAATGACAATCAGGAGTTACAAAAACTTAAATCTGGTCAGGGCa  <  2:320810/139‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                      
TGTCTGCTGGCGAATAATTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGAAATGACAATCAGGAGTTACAAAAACTTAAATCTGGTCAGGGCA  >  NC_000913/2765689‑2765959

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: