Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,782,393 (C)5→4 coding (334/4581 nt) ypjA ← adhesin‑like autotransporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,782,3890C.98.1% 440.3 / 1.2 105coding (338/4581 nt)ypjAadhesin‑like autotransporter
Reads supporting (aligned to +/- strand):  ref base C (1/1);  new base . (51/52);  total (52/53)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.14e-01

Only 100 of 114 total aligned reads displayed.

TACCACCATCAATGAGTATTCCATTCGCACTCCCTCCCTCTACGCGTGAAGCACCGCCCTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATACCCCCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTATGACTTCCTATATCTTGATATCCACCAGATTCAATAAGACTTCCATTAGATACCCCGCCATGAA  >  NC_000913/2782252‑2782521
                                                                                                                                         |                                                                                                                                    
tACCACCATCAATGAGTATTCCATTCGCACTCCCTCCCTCTACGCGTGAAGCACCGCCCTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATAcc                                                                                                                                     <  2:1064271/139‑1 (MQ=255)
        tCAATGAGTATTCCATTCGCACTCCCTCCCTCTACGCGTGAAGCACCGCCCTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATACCCCGTCATg                                                                                                                            >  2:1112358/1‑139 (MQ=255)
        tCAATGAGTATTCCATTCGCACTCCCTCCCTCTACGCGTGAAGCACCGCCCTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATACCCCGTCATg                                                                                                                            >  2:773705/1‑139 (MQ=255)
        tCAATGAGTATTCCATTCGCACTCCCTCCCTCTACGCGTGAAGCACCGCCCTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATACCCCGTCATg                                                                                                                            >  2:805618/1‑139 (MQ=255)
        tCAATGAGTATTCCATTCGCACTCCCTCCCTCTACGCGTGAAGCACCGCCCTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATACCCCGTCATg                                                                                                                            >  2:207154/1‑139 (MQ=255)
            tGAGTATTCCATTCGCACTCCCTCCCTCTACGCGTGAAGCACCGCCCTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATACCCCGTCATGAAtt                                                                                                                        <  2:28971/139‑1 (MQ=255)
              aGTATTCCATTCGCACTCCCTCCCTCTACGCGTGAAGCACCGCCCTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATACCCCGTCATGAATTGa                                                                                                                      <  1:805618/139‑1 (MQ=255)
                tATTCCATTCGCACTCCCTCCCTCTACGCGTGAAGCACCGCCCTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATACCCCGTCATGAATTGACt                                                                                                                    >  1:853203/1‑139 (MQ=255)
                  ttCCATTCGCACTCCCTCCCTCTACGCGTGAAGCACCGCCCTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATACCCCGTCATGAATTGACTGt                                                                                                                  >  1:848626/1‑139 (MQ=255)
                   tCCATTCGCACTCCCTCCCTCTACGCGTGAAGCACCGCCCTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATACCCCGTCATGAATTGACTGTc                                                                                                                 >  1:833158/1‑139 (MQ=255)
                    ccATTCGCACTCCCTCCCTCTACGCGTGAAGCACCGCCCTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATACCCCGTCATAAATTGACTGTCt                                                                                                                >  1:237708/1‑139 (MQ=255)
                        tCGCACTCCCTCCCTCTACGCGTGAAGCACCGCCCTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATACCCCGTCATGAATTGACTGTCTGCCa                                                                                                            <  1:207154/139‑1 (MQ=255)
                          gCACTCCCTCCCTCTACGCGTGAAGCACCGCCCTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATACCCCGTCATGAATTGACTGTCTGCCAcc                                                                                                          <  1:1058423/139‑1 (MQ=255)
                          gCACTCCCTCCCTCTACGCGTGAAGCACCGCCCTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATACCCCGTCATGAATTGACTGTCTGCCAcc                                                                                                          >  2:718780/1‑139 (MQ=255)
                            aCTCCCTCCCTCTACGCGTGAAGCACCGCCCTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGCTTGTCGTACCTGTGGAAATACCCCGTCATGAATTGACTGTCTGCCACCGt                                                                                                        >  1:862797/1‑139 (MQ=255)
                               ccctccctCTACGCGTGAAGCACCGCCCTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATACCCCGTCATGAATTGACTGTCTGCCACCGTTaa                                                                                                     >  1:573074/1‑139 (MQ=255)
                               ccctccctCTACGCGTGAAGCACCGCCCTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATACCCCGTCATGAATTGACTGTCTGCCACCGTTaa                                                                                                     <  1:689751/139‑1 (MQ=255)
                               ccctccctCTACGCGTGAAGCACCGCCCTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATACCCCGTCATGAATTGACTGTCTGCCACCGTTaa                                                                                                     <  1:21125/139‑1 (MQ=255)
                                     ctctACGCGTGAAGCACCGCCCTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATACCCCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTg                                                                                               <  2:485698/139‑1 (MQ=255)
                                     ctctACGCGTGAAGCACCGCCCTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATACCCCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTg                                                                                               <  2:862797/139‑1 (MQ=255)
                                     ctctACGCGTGAAGCACCGCCCTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATACCCCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTg                                                                                               <  1:410056/139‑1 (MQ=255)
                                     ctctACGCGTGAAGCACCGCCCTTAATAGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATACCCCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTg                                                                                               <  2:640814/139‑1 (MQ=255)
                                          cgcgTGAAGCACCGCCCTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATAccccgt                                                                                                                                 >  2:584315/1‑99 (MQ=255)
                                          cgcgTGAAGCACCGCCCTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATAccccgt                                                                                                                                 <  1:584315/101‑3 (MQ=255)
                                          cgcgTGAAGCACCGCCCTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATACCCCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGtatta                                                                                          >  2:32453/1‑139 (MQ=255)
                                          cgcgTGAAGCACCGCCCTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATACCCCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGtatta                                                                                          >  2:1063369/1‑139 (MQ=255)
                                          cgcgTGAAGCACCGCCCTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATACCCCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGtatta                                                                                          >  1:167559/1‑139 (MQ=255)
                                              tGAAGCACCGCCCTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATAcccc                                                                                                                                   >  1:141869/1‑95 (MQ=255)
                                              tGAAGCACCGCCCTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATAcccc                                                                                                                                   <  2:141869/95‑1 (MQ=255)
                                                aaGCACCGCCCTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATACCCCCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGc                                                                                     >  1:883392/1‑139 (MQ=255)
                                                aaGCACCGCCCTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATACCCCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGcc                                                                                    <  2:573074/139‑1 (MQ=255)
                                                  gCACCGCCCTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATACCCCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTg                                                                                  <  1:1063369/139‑1 (MQ=255)
                                                    aCCGCCCTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATACCCCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGcc                                                                                >  2:1064385/1‑139 (MQ=255)
                                                     ccGCCCTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATACCCCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGccc                                                                               >  1:816680/1‑139 (MQ=255)
                                                        cccTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATACCCCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCcac                                                                            >  2:810796/1‑139 (MQ=255)
                                                        cccTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATACCCCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCca                                                                             >  1:500435/1‑138 (MQ=255)
                                                        cccTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATACCCCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCca                                                                             <  2:500435/138‑1 (MQ=255)
                                                                  tCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATACCCCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTa                                                                  <  2:237708/139‑1 (MQ=255)
                                                                    gTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATACCCCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTATg                                                                >  1:1011762/1‑139 (MQ=255)
                                                                     ttCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATACCCCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTATGa                                                               <  1:394364/139‑1 (MQ=255)
                                                                     ttCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATACCCCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTATGa                                                               >  1:374059/1‑139 (MQ=255)
                                                                      tCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATACCCCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTATGAc                                                              <  2:1011762/139‑1 (MQ=255)
                                                                      tCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATACCCCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTATGAc                                                              <  1:32453/139‑1 (MQ=255)
                                                                       ccATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATACCCCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTAt                                                                 <  1:610416/135‑1 (MQ=255)
                                                                       ccATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATACCCCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTATGa                                                               >  2:244478/1‑137 (MQ=255)
                                                                       ccATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATACCCCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTATGa                                                               <  1:244478/137‑1 (MQ=255)
                                                                          ttGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATACCCCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTATGACTTcc                                                          <  1:1064385/139‑1 (MQ=255)
                                                                             cTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATACCCCCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTATGACTTCCta                                                        <  2:883392/139‑1 (MQ=255)
                                                                             cTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATACCCCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTGTGACTTCCtat                                                       >  1:724917/1‑139 (MQ=255)
                                                                                aTACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATACCCCGTCATGAATTGACTGTCTGc                                                                                                              <  1:1011743/81‑1 (MQ=255)
                                                                                aTACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATACCCCGTCATGAATTGACTGTCTGc                                                                                                              >  2:1011743/1‑81 (MQ=255)
                                                                                aTACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATACCCCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTATGACTTCCTATATc                                                    >  1:446029/1‑139 (MQ=255)
                                                                                aTACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATACCCCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTATGACTTCCTATATc                                                    >  2:224577/1‑139 (MQ=255)
                                                                                 tACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATACCCCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTATGACTTCCTATATCt                                                   >  1:815783/1‑139 (MQ=255)
                                                                                     cccTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATACCCCGTCATGAATTGACTGTCTGCCACCGTTaa                                                                                                     <  2:496755/85‑1 (MQ=255)
                                                                                     cccTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATACCCCGTCATGAATTGACTGTCTGCCACCGTTaa                                                                                                     >  1:496755/1‑85 (MQ=255)
                                                                                     cccTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATACCCCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTAt                                                                 >  2:1092091/1‑121 (MQ=255)
                                                                                     cccTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATACCCCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTAt                                                                 <  1:1092091/121‑1 (MQ=255)
                                                                                      ccTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATACCCCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTATGACTTCCTATATCTTGata                                              <  2:833158/139‑1 (MQ=255)
                                                                                       cTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATAccccgtca                                                                                                                               <  2:1020385/58‑5 (MQ=255)
                                                                                       cTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATAccccgtca                                                                                                                               >  1:1020385/1‑54 (MQ=255)
                                                                                       cTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATACCCCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTATGACTTCCTATATCTTGatat                                             >  2:264212/1‑139 (MQ=255)
                                                                                               aCGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATACCCCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTATGACTTCCTATATCTTGATATCCACCAGa                                     <  1:446226/139‑1 (MQ=255)
                                                                                                cGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATACCCCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTATGACTTCCtata                                                      <  1:219670/121‑1 (MQ=255)
                                                                                                cGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATACCCCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTATGACTTCCtata                                                      >  2:219670/1‑121 (MQ=255)
                                                                                                cGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATACCCCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTATGACTTCCTATATCTTGATATCCACCAGAt                                    >  1:120839/1‑139 (MQ=255)
                                                                                                 gTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATAccccgt                                                                                                                                 >  1:161791/1‑44 (MQ=255)
                                                                                                 gTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATAccccgt                                                                                                                                 <  2:161791/46‑3 (MQ=255)
                                                                                                  tCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATACCCCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTATGACTTCCTATATCTTGATATCCACCAGATTc                                  <  2:120839/139‑1 (MQ=255)
                                                                                                    cTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATACCCCGTCATGAATTGACTGTCTGCCACCGTTaa                                                                                                     >  2:106517/1‑70 (MQ=255)
                                                                                                    cTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATACCCCGTCATGAATTGACTGTCTGCCACCGTTaa                                                                                                     <  1:106517/70‑1 (MQ=255)
                                                                                                    cTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATACCCCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTATGACTTCCTATATCTTGATATCCACCAGATTCaa                                >  1:583753/1‑139 (MQ=255)
                                                                                                      gATTGCCACTCTCGATTGTCGTACCTGTGGAAATACCCCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTATGACTTCCTATATCTTGATATCCACCAGa                                     >  1:907882/1‑132 (MQ=255)
                                                                                                       aTTGCCACTCTCGATTGTCGTACCTGTGGAAATACCCCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTATGACTTCCTATATCTTGATATCCACCAGa                                     <  1:172039/131‑1 (MQ=255)
                                                                                                       aTTGCCACTCTCGATTGTCGTACCTGTGGAAATACCCCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTATGACTTCCTATATCTTGATATCCACCAGa                                     >  2:172039/1‑131 (MQ=255)
                                                                                                          gCCACTCTCGATTGTCGTACCTGTGGAAATACCCCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTATGACTTCCTATATCTTGATATCCACCAGATTCAATAAGAc                          <  2:816680/139‑1 (MQ=255)
                                                                                                          gCCACTCTCGATTGTCGTACCTGTGGAAATACCCCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTATGACTTCCTATATCTTGATATCCACCAGATTCAATAAGAc                          <  1:810796/139‑1 (MQ=255)
                                                                                                          gCCACTCTCGATTGTCGTACCTGTGGAAATACCCCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTATGACTTCCTATATCTTGATATCCACCAGATTCAATAAGAc                          <  2:724917/139‑1 (MQ=255)
                                                                                                          gCCACTCTCGATTGTCGTACCTGTGGAAATACCCCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTATGACTTCCTATATCTTGATATCCACCAGATTCAATAAGAc                          <  1:224577/139‑1 (MQ=255)
                                                                                                          gCCACTCTCGATTGTCGTACCTGTGGAAATACCCCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTATGACTTCCTATATCTTGATATCCACCAGATTCAATAAGAc                          <  1:451253/139‑1 (MQ=255)
                                                                                                          gCCACTCTCGATTGTCGTACCTGTGGAAATACCCCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTATGACTTCCTATATCTTGATATCCACCAGATTCAATAAGAc                          <  1:635233/139‑1 (MQ=255)
                                                                                                          gCCACTCTCGATTGTCGTACCTGTGGAAATACCCCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTATGACTTCCTATATCTTGATATCCACCAGATTCAATAAGAc                          <  2:167559/139‑1 (MQ=255)
                                                                                                             aCTCTCGATTGTCGTACCTGTGGAAATACCCCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTATGACTTCCTATATCTTGATATCCACCAGATTCAATAAGACTTc                       >  1:788910/1‑139 (MQ=255)
                                                                                                                        tCGTACCTGTGGAAATACCCCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACaaa                                                                         >  2:768934/1‑78 (MQ=255)
                                                                                                                        tCGTACCTGTGGAAATACCCCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTATGACTTCCTATATCTTGATATCCACCAGATTCAATAAGACTTCCATTAGATAcc            <  1:718780/139‑1 (MQ=255)
                                                                                                                         cGTACCTGTGGAAATACCCCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTATGAc                                                              >  1:115190/1‑88 (MQ=255)
                                                                                                                           tACCTGTGGAAATACCCCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTATGACTTCCTATATCTTGATATCCACCAGATTCAATAAGACTTCCATTAGATACCCCg         >  1:65461/1‑139 (MQ=255)
                                                                                                                            aCCTGTGGAAATACCCCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTATGACTTCCTATATCTTGATATCCACCAGATTCAATAAGACTTCCATTAGATACCCCGc        <  1:264212/139‑1 (MQ=255)
                                                                                                                                 tgGAAATACCCCGTCATGAATTGACTGTCTGCCACCGTTAAt                                                                                                    >  1:550777/1‑42 (MQ=255)
                                                                                                                                 tgGAAATACCCCGTCATGAATTGACTGTCTGCCACCGTTAAt                                                                                                    <  2:550777/42‑1 (MQ=255)
                                                                                                                                 tgGAAATACCCCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACaaa                                                                         >  2:1004285/1‑69 (MQ=255)
                                                                                                                                 tgGAAATACCCCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACaaa                                                                         <  1:1004285/69‑1 (MQ=255)
                                                                                                                                  ggAAATACCCCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTATGAc                                                              >  2:71786/1‑79 (MQ=255)
                                                                                                                                  ggAAATACCCCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTATGAc                                                              <  1:71786/79‑1 (MQ=255)
                                                                                                                                  ggAAATACCCCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTATGACTTCCta                                                        <  2:206799/85‑1 (MQ=255)
                                                                                                                                  ggAAATACCCCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTATGACTTCCTATATCTTGATATccacc                                        >  2:849846/1‑101 (MQ=255)
                                                                                                                                  ggAAATACCCCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTATGACTTCCTATATCTTGATATCCACCAGATTCAATAAGACTTCCATTAGATACCCCGCCa      <  1:50544/135‑1 (MQ=255)
                                                                                                                                  ggAAATACCCCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTATGACTTCCTATATCTTGATATCCACCAGATTCAATAAGACTTCCATTAGATACCCCGCCa      >  2:50544/1‑135 (MQ=255)
                                                                                                                                  ggAAATACCCCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTATGACTTCCTATATCTTGATATCCACCAGATTCAATAAGACTTCCATTAGATACCCCGCCATGaa  >  2:659536/1‑139 (MQ=255)
                                                                                                                                   gAAATACCCCGTCATGAATTGACTGTCTGCCACCGTTaa                                                                                                     >  2:1087271/1‑39 (MQ=255)
                                                                                                                                         |                                                                                                                                    
TACCACCATCAATGAGTATTCCATTCGCACTCCCTCCCTCTACGCGTGAAGCACCGCCCTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATACCCCCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTATGACTTCCTATATCTTGATATCCACCAGATTCAATAAGACTTCCATTAGATACCCCGCCATGAA  >  NC_000913/2782252‑2782521

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: