Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 281,662 Δ1 bp coding (322/1155 nt) yagA ← CP4‑6 prophage, putative DNA‑binding transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913281,6600G.100.0% 119.8 / NA 29coding (324/1155 nt)yagACP4‑6 prophage, putative DNA‑binding transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base . (14/15);  total (14/15)

TAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCGGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCGGGCATGGTGTGCCCCTGGTCCTCGAGCCAGCGCTTAATCTTGCGGGCTCCCCAGCGTTCATGACGGTCATGGGCCATACGCAGCAGGGCCGTGATGTCGTCAGATGAGCGGTTCGGGGAATGGTGCGG  >  NC_000913/281536‑281788
                                                                                                                            |                                                                                                                                
tAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCGGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCGGCATGGTGTGcccc                                                                                                                   <  1:874362/139‑1 (MQ=255)
             ccAGAGGCGGTTCGGCGCGTCGTGTTCGAACCGGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCGGCATGGTGTGCCCCTGGTCCTCGAGcc                                                                                                      >  2:676708/1‑139 (MQ=255)
              cAGAGGCGGTTCGGCGCGTCGTGTTCGAACCGGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCGGCATGGTGTGCCCCTGGTCCTCGAGCCa                                                                                                     <  1:314964/139‑1 (MQ=255)
                    cGGTTCGGCGCGTCGTGTTCGAACCGGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGAAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCGGCATGGTGTGCCCCTGGTCCTCGAGCCAGCGGtt                                                                                               >  2:48875/1‑139 (MQ=255)
                               gtcgtGTTCGAACCGGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCGGCATGGTGTGCCCCTGGTCCTCGAGCCAGCGCTTAATCTTGCggg                                                                                    <  1:51910/139‑1 (MQ=255)
                                tcgtGTTCGAACCGGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCGGCATGGTGTGCCCCTGGTCCTCGAGCCAGCGCTTAATCTTGCGGGc                                                                                   >  2:152027/1‑139 (MQ=255)
                                     ttCGAACCGGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCGGCATGGTGTGCCCCTGGTCCTCGAGCCAGCGCTTAATCTTGCGGGCTcccc                                                                              <  2:128707/139‑1 (MQ=255)
                                             ggCCCGTCGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTACGGACGGTGCTGAAGGCGGCATGGTGTGCCCCTGGTCCTCGAGCCAGCGCTTAATCTGGCGGGCTCCCCAGCGTTCa                                                                      >  2:727245/1‑139 (MQ=255)
                                               cccGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCGGCATGGTGTGcccc                                                                                                                   <  1:118728/92‑1 (MQ=255)
                                               cccGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCGGCATGGTGTGcccc                                                                                                                   >  2:118728/1‑92 (MQ=255)
                                               cccGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCGGCATGGTGTGCCCCTGGTCCTCGAGCCAGCGCTTAATCTTGCGGGCTCCCCAGCGTTCATg                                                                    >  1:525358/1‑139 (MQ=255)
                                                ccGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCGGCATGGTGTGCCCCTGGTCCTCGAGCCAGCGCTTAATCTTGCGGGCTCCCCAGCGTTCATGa                                                                   <  1:681491/139‑1 (MQ=255)
                                                                       gcgcCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCGGCATGGTGTGCCTCTGGTCCTCGAGCCAGCGCTTAATCTTGCGGGCTCCCCAGCGTTCATGACGGTCATGCGCCATACGcagcag                                            <  1:676708/139‑1 (MQ=255)
                                                                       gcgcCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCGGCATGGTGTGCCCCTGGTCCTCGAGCCAGCGCTTAATCTTGCGGGCTCCCCAGCGTTCATGa                                                                   <  2:384334/116‑1 (MQ=255)
                                                                       gcgcCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCGGCATGGTGTGCCCCTGGTCCTCGAGCCAGCGCTTAATCTTGCGGGCTCCCCAGCGTTCATGa                                                                   >  1:384334/1‑116 (MQ=255)
                                                                             ggCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCGGCATGGTGTGCCCCTGGTCCTCGAGcc                                                                                                      <  2:1021662/75‑1 (MQ=255)
                                                                             ggCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCGGCATGGTGTGCCCCTGGTCCTCGAGcc                                                                                                      >  1:1021662/1‑75 (MQ=255)
                                                                              gcagcaGGGCAAGACGGGCCATCAGGTTATGGACGGTGCTGAAGGCGGCATGGTGTGACCCTGGTCCTCGAGCCAGGGCTTAATCTTGCAGGCTCCCCAGCGTTCATGACGGTCATGGGCCATACGCCGCATGGcggag                                     <  2:173620/139‑5 (MQ=255)
                                                                              gcagcaGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCGGCATGGTGTGCCCCTGGTCCTCGAGCCAGCGCTTAATCTTGCGGGCTCCCCAGCGTTCATGACGGTCATGGGCCATACGCAGCAGGGCCGTg                                     <  1:152027/139‑1 (MQ=255)
                                                                               cagcagGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCGGCGTGGTGTGCCCCTGGTCCTCGAGCCAGCGCTTAATCtt                                                                                         <  1:905173/86‑1 (MQ=255)
                                                                               cagcagGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCGGCATGGTGTGCCCCTGGTCCTCGAGCCAGCGCTTAATCtt                                                                                         >  2:905173/1‑86 (MQ=255)
                                                                                            cGGGCCATCAGGTTATGGACGGTGCTGAAGGCGGCATGGTGTGCCCCTGGTCCTCGAGCCAGCGCTTAATCTTGCGGGCTCCCCAGCGTTCATGACGGTCATGGGCCATACGCAGCATGGCCGTGATGTCGTCAGATGa                       >  1:310630/1‑139 (MQ=255)
                                                                                               gCCATCAGGTTATGGACGGTGCTGAAGGCGGCATGGTGTGCCCCTGGTCCTCGAGCCAGCGCTTAATCTTGCGGGCTCCCCAGCGTTCATGACGGTCATGGGCCATACgcag                                               <  2:1103376/112‑1 (MQ=255)
                                                                                               gCCATCAGGTTATGGACGGTGCTGAAGGCGGCATGGTGTGCCCCTGGTCCTCGAGCCAGCGCTTAATCTTGCGGGCTCCCCAGCGTTCATGACGGTCATGGGCCATACgcag                                               >  1:1103376/1‑112 (MQ=255)
                                                                                                   tCAGGTTATGGACGGTGCTGAAGGCGGCATGGTGTGCCCCTGGTCCTCGAGCCAGCGCTTAATCTTGCGGGCTCCCCAGCGTTCATGACGGTCATGGGCCATACGCAGCAGGGCCGTGATGTCGTCAGATGAGCGGTTc                >  1:130085/1‑139 (MQ=255)
                                                                                                                 gTGCTGAAGGCGGCATGGTGTGCCCCTGGTCCTCGAGCCAGCGCTTAATCTTGCGGGCTCCCCAGCGTTCATGACGGTCATGGGCCATACGCAGCAGGGCCGTGATGTCGTCAGATGAGCGGTTCg               <  2:1056117/126‑1 (MQ=255)
                                                                                                                 gTGCTGAAGGCGGCATGGTGTGCCCCTGGTCCTCGAGCCAGCGCTTAATCTTGCGGGCTCCCCAGCGTTCATGACGGTCATGGGCCATACGCAGCAGGGCCGTGATGTCGTCAGATGAGCGGTTCg               >  1:1056117/1‑126 (MQ=255)
                                                                                                                 gTGCTGAAGGCGGCATGGTGTGCCCCTGGTCCTCGAGCCAGCGCTTAATCTTGCGGGCTCCCCAGCGTTCATGACGGTCATGGGCCATACGCAGCAGGGCCGTGATGTCGTCAGATGAGCGGTTCGGGGAATGGTGCgg  >  1:657/1‑139 (MQ=255)
                                                                                                                 gTGCTGAAGGCGGCATGGTGTGCCCCTGGTCCTCGAGCCAGCGCTTAATCTTGCGGGCTCCCCAGCGTTCATGACGGTCATGGGCCATACGCAGCAGGGCCGTGATGTCGTCAGATGAGCGGTTCGGGGAATGGTGCgg  <  2:525358/139‑1 (MQ=255)
                                                                                                                            |                                                                                                                                
TAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCGGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCGGGCATGGTGTGCCCCTGGTCCTCGAGCCAGCGCTTAATCTTGCGGGCTCCCCAGCGTTCATGACGGTCATGGGCCATACGCAGCAGGGCCGTGATGTCGTCAGATGAGCGGTTCGGGGAATGGTGCGG  >  NC_000913/281536‑281788

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: